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Protein

Nucleoporin NUP85

Gene

NUP85

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. NUP85 is involved in nuclear poly(A)+ RNA and pre-ribosome export, in GSP1 nuclear import, in NPC assembly and distribution, as well as in nuclear envelope organization.6 Publications

GO - Molecular functioni

  • structural constituent of nuclear pore Source: SGD

GO - Biological processi

  • mRNA export from nucleus Source: SGD
  • nuclear pore distribution Source: SGD
  • positive regulation of transcription, DNA-templated Source: SGD
  • protein import into nucleus Source: SGD
  • ribosomal large subunit export from nucleus Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31677-MONOMER.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoporin NUP85
Alternative name(s):
Nuclear pore protein NUP85
Gene namesi
Name:NUP85
Synonyms:RAT9
Ordered Locus Names:YJR042W
ORF Names:J1624
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR042W.
SGDiS000003803. NUP85.

Subcellular locationi

GO - Cellular componenti

  • nuclear membrane Source: UniProtKB-SubCell
  • nuclear pore Source: SGD
  • nuclear pore outer ring Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 744744Nucleoporin NUP85PRO_0000204885Add
BLAST

Proteomic databases

MaxQBiP46673.

PTM databases

iPTMnetiP46673.

Interactioni

Subunit structurei

The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEH1 and SEC1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. NUP85 is part of the heptameric 0.5 MDa autoassembling NUP84 NPC subcomplex (NUP84, NUP85, NUP120, NUP133, NUP145C, SEC13 and SEH1). NUP85 also interacts directly with the GLFG repeats of NUP116 and directly or indirectly with the mRNA transport factor MTR2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NUP120P357293EBI-12345,EBI-11713
NUP145P496873EBI-12345,EBI-11730
SEH1P530113EBI-12345,EBI-16940

Protein-protein interaction databases

BioGridi33793. 42 interactions.
DIPiDIP-5818N.
IntActiP46673. 16 interactions.
MINTiMINT-648452.

Structurei

Secondary structure

1
744
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi41 – 5111Combined sources
Beta strandi67 – 748Combined sources
Beta strandi77 – 826Combined sources
Beta strandi89 – 913Combined sources
Helixi102 – 11716Combined sources
Helixi119 – 1224Combined sources
Helixi135 – 16430Combined sources
Helixi170 – 18920Combined sources
Beta strandi190 – 1923Combined sources
Helixi197 – 2004Combined sources
Helixi201 – 21515Combined sources
Helixi221 – 2277Combined sources
Beta strandi237 – 2404Combined sources
Helixi243 – 25412Combined sources
Helixi258 – 2658Combined sources
Turni266 – 2716Combined sources
Helixi272 – 2765Combined sources
Helixi279 – 29214Combined sources
Helixi300 – 31819Combined sources
Helixi326 – 34015Combined sources
Helixi343 – 3486Combined sources
Helixi353 – 36311Combined sources
Helixi368 – 3703Combined sources
Helixi371 – 38111Combined sources
Beta strandi388 – 3903Combined sources
Helixi391 – 3988Combined sources
Helixi403 – 4053Combined sources
Helixi406 – 4127Combined sources
Helixi414 – 42714Combined sources
Beta strandi454 – 4596Combined sources
Helixi461 – 47414Combined sources
Turni479 – 4813Combined sources
Helixi482 – 49110Combined sources
Beta strandi493 – 4953Combined sources
Helixi497 – 50711Combined sources
Helixi508 – 5103Combined sources
Helixi516 – 52914Combined sources
Helixi532 – 54312Combined sources
Helixi545 – 5484Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3EWEX-ray3.50B/D1-564[»]
3F3FX-ray2.90C/D/G/H1-570[»]
3F3GX-ray3.75C/D/G/H1-570[»]
3F3PX-ray3.20C/D/G/H/K/L1-570[»]
4XMMX-ray7.38D44-744[»]
4XMNX-ray7.60D73-743[»]
ProteinModelPortaliP46673.
SMRiP46673. Positions 44-744.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP46673.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi646 – 6527Poly-Leu

Sequence similaritiesi

Belongs to the nucleoporin Nup85 family.Curated

Phylogenomic databases

HOGENOMiHOG000113893.
InParanoidiP46673.
KOiK14304.
OMAiTNDDIEW.
OrthoDBiEOG092C2XAD.

Family and domain databases

InterProiIPR011502. Nucleoporin_Nup85.
[Graphical view]
PfamiPF07575. Nucleopor_Nup85. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46673-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIDDSNRLL MDVDQFDFLD DGTAQLSNNK TDEEEQLYKR DPVSGAILVP
60 70 80 90 100
MTVNDQPIEK NGDKMPLKFK LGPLSYQNMA FITAKDKYKL YPVRIPRLDT
110 120 130 140 150
SKEFSAYVSG LFEIYRDLGD DRVFNVPTIG VVNSNFAKEH NATVNLAMEA
160 170 180 190 200
ILNELEVFIG RVKDQDGRVN RFYELEESLT VLNCLRTMYF ILDGQDVEEN
210 220 230 240 250
RSEFIESLLN WINRSDGEPD EEYIEQVFSV KDSTAGKKVF ETQYFWKLLN
260 270 280 290 300
QLVLRGLLSQ AIGCIERSDL LPYLSDTCAV SFDAVSDSIE LLKQYPKDSS
310 320 330 340 350
STFREWKNLV LKLSQAFGSS ATDISGELRD YIEDFLLVIG GNQRKILQYS
360 370 380 390 400
RTWYESFCGF LLYYIPSLEL SAEYLQMSLE ANVVDITNDW EQPCVDIISG
410 420 430 440 450
KIHSILPVME SLDSCTAAFT AMICEAKGLI ENIFEGEKNS DDYSNEDNEM
460 470 480 490 500
LEDLFSYRNG MASYMLNSFA FELCSLGDKE LWPVAIGLIA LSATGTRSAK
510 520 530 540 550
KMVIAELLPH YPFVTNDDIE WMLSICVEWR LPEIAKEIYT TLGNQMLSAH
560 570 580 590 600
NIIESIANFS RAGKYELVKS YSWLLFEASC MEGQKLDDPV LNAIVSKNSP
610 620 630 640 650
AEDDVIIPQD ILDCVVTNSM RQTLAPYAVL SQFYELRDRE DWGQALRLLL
660 670 680 690 700
LLIEFPYLPK HYLVLLVAKF LYPIFLLDDK KLMDEDSVAT VIEVIETKWD
710 720 730 740
DADEKSSNLY ETIIEADKSL PSSMATLLKN LRKKLNFKLC QAFM
Length:744
Mass (Da):84,898
Last modified:November 1, 1995 - v1
Checksum:i4C0A4AD3DB57A023
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti101 – 1011S → T in AAB48943 (PubMed:8816998).Curated
Sequence conflicti471 – 4711F → L in AAB48943 (PubMed:8816998).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36469 Genomic DNA. Translation: AAB48943.1.
X90995 Genomic DNA. Translation: CAA62481.1.
L36344 Genomic DNA. Translation: AAA88744.1.
Z49542 Genomic DNA. Translation: CAA89569.1.
BK006943 Genomic DNA. Translation: DAA08829.1.
PIRiS57061.
RefSeqiNP_012576.1. NM_001181700.1.

Genome annotation databases

EnsemblFungiiYJR042W; YJR042W; YJR042W.
GeneIDi853499.
KEGGisce:YJR042W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36469 Genomic DNA. Translation: AAB48943.1.
X90995 Genomic DNA. Translation: CAA62481.1.
L36344 Genomic DNA. Translation: AAA88744.1.
Z49542 Genomic DNA. Translation: CAA89569.1.
BK006943 Genomic DNA. Translation: DAA08829.1.
PIRiS57061.
RefSeqiNP_012576.1. NM_001181700.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3EWEX-ray3.50B/D1-564[»]
3F3FX-ray2.90C/D/G/H1-570[»]
3F3GX-ray3.75C/D/G/H1-570[»]
3F3PX-ray3.20C/D/G/H/K/L1-570[»]
4XMMX-ray7.38D44-744[»]
4XMNX-ray7.60D73-743[»]
ProteinModelPortaliP46673.
SMRiP46673. Positions 44-744.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33793. 42 interactions.
DIPiDIP-5818N.
IntActiP46673. 16 interactions.
MINTiMINT-648452.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

PTM databases

iPTMnetiP46673.

Proteomic databases

MaxQBiP46673.

Protocols and materials databases

DNASUi853499.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR042W; YJR042W; YJR042W.
GeneIDi853499.
KEGGisce:YJR042W.

Organism-specific databases

EuPathDBiFungiDB:YJR042W.
SGDiS000003803. NUP85.

Phylogenomic databases

HOGENOMiHOG000113893.
InParanoidiP46673.
KOiK14304.
OMAiTNDDIEW.
OrthoDBiEOG092C2XAD.

Enzyme and pathway databases

BioCyciYEAST:G3O-31677-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP46673.
PROiP46673.

Family and domain databases

InterProiIPR011502. Nucleoporin_Nup85.
[Graphical view]
PfamiPF07575. Nucleopor_Nup85. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUP85_YEAST
AccessioniPrimary (citable) accession number: P46673
Secondary accession number(s): D6VWL3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7920 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.