Nucleoporin NUP85 - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P46673 (NUP85_YEAST)

Last modified November 24, 2009. Version 82. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Nucleoporin NUP85
Alternative name(s):
Nuclear pore protein NUP85

Gene names

Name:	NUP85
Synonyms:	RAT9
Ordered Locus Names:	YJR042W
ORF Names:	J1624

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	744 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. NUP85 is involved in nuclear poly(A)+ RNA and pre-ribosome export, in GSP1 nuclear import, in NPC assembly and distribution, as well as in nuclear envelope organization. Ref.1 Ref.5 Ref.7 Ref.8 Ref.9 Ref.10
Subunit structure	The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEH1 and SEC1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. NUP85 is part of the heptameric 0.5 MDa autoassembling NUP84 NPC subcomplex (NUP84, NUP85, NUP120, NUP133, NUP145C, SEC13 and SEH1). NUP85 also interacts directly with the GLFG repeats of NUP116 and directly or indirectly with the mRNA transport factor MTR2. Ref.5 Ref.8 Ref.6
Subcellular location	Nucleus › nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. Note: Symmetric distribution.
Miscellaneous	Present with 7920 molecules/cell in log phase SD medium. Ref.11
Sequence similarities	Belongs to the nucleoporin Nup85 family.

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Ontologies

Keywords
Biological process	Protein transport Translocation Transport mRNA transport
Cellular component	Membrane Nuclear pore complex Nucleus
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	NLS-bearing substrate import into nucleus Traceable author statement. Source: SGD mRNA export from nucleus Inferred from mutant phenotype. Source: SGD mRNA-binding (hnRNP) protein import into nucleus Traceable author statement. Source: SGD nuclear envelope organization Inferred from mutant phenotype. Source: SGD nuclear pore organization Traceable author statement. Source: SGD protein export from nucleus Traceable author statement. Source: SGD rRNA export from nucleus Traceable author statement. Source: SGD ribosomal protein import into nucleus Traceable author statement. Source: SGD snRNA export from nucleus Traceable author statement. Source: SGD snRNP protein import into nucleus Traceable author statement. Source: SGD tRNA export from nucleus Traceable author statement. Source: SGD transmembrane transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Nup107-160 complex Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct structural molecule activity Traceable author statement. Source: SGD
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
HSP12	P22943	1	EBI-12345,EBI-8548
MBF1	O14467	1	EBI-12345,EBI-2043025
NUP116	Q02630	1	EBI-12345,EBI-11703
NUP133	P36161	1	EBI-12345,EBI-11722
NUP145	P49687	1	EBI-12345,EBI-11730
NUP2	P32499	1	EBI-12345,EBI-12401
NUP82	P40368	1	EBI-12345,EBI-12331
RTN1	Q04947	1	EBI-12345,EBI-38020

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 744

744

Nucleoporin NUP85

PRO_0000204885

Regions

Compositional bias

646 – 652

Poly-Leu

Experimental info

Sequence conflict

101

S → T in AAB48943. Ref.1

Sequence conflict

471

F → L in AAB48943. Ref.1

Secondary structure

744

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P46673-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 4C0A4AD3DB57A023
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FASTA

744

84,898

        10         20         30         40         50         60 
MTIDDSNRLL MDVDQFDFLD DGTAQLSNNK TDEEEQLYKR DPVSGAILVP MTVNDQPIEK 

        70         80         90        100        110        120 
NGDKMPLKFK LGPLSYQNMA FITAKDKYKL YPVRIPRLDT SKEFSAYVSG LFEIYRDLGD 

       130        140        150        160        170        180 
DRVFNVPTIG VVNSNFAKEH NATVNLAMEA ILNELEVFIG RVKDQDGRVN RFYELEESLT 

       190        200        210        220        230        240 
VLNCLRTMYF ILDGQDVEEN RSEFIESLLN WINRSDGEPD EEYIEQVFSV KDSTAGKKVF 

       250        260        270        280        290        300 
ETQYFWKLLN QLVLRGLLSQ AIGCIERSDL LPYLSDTCAV SFDAVSDSIE LLKQYPKDSS 

       310        320        330        340        350        360 
STFREWKNLV LKLSQAFGSS ATDISGELRD YIEDFLLVIG GNQRKILQYS RTWYESFCGF 

       370        380        390        400        410        420 
LLYYIPSLEL SAEYLQMSLE ANVVDITNDW EQPCVDIISG KIHSILPVME SLDSCTAAFT 

       430        440        450        460        470        480 
AMICEAKGLI ENIFEGEKNS DDYSNEDNEM LEDLFSYRNG MASYMLNSFA FELCSLGDKE 

       490        500        510        520        530        540 
LWPVAIGLIA LSATGTRSAK KMVIAELLPH YPFVTNDDIE WMLSICVEWR LPEIAKEIYT 

       550        560        570        580        590        600 
TLGNQMLSAH NIIESIANFS RAGKYELVKS YSWLLFEASC MEGQKLDDPV LNAIVSKNSP 

       610        620        630        640        650        660 
AEDDVIIPQD ILDCVVTNSM RQTLAPYAVL SQFYELRDRE DWGQALRLLL LLIEFPYLPK 

       670        680        690        700        710        720 
HYLVLLVAKF LYPIFLLDDK KLMDEDSVAT VIEVIETKWD DADEKSSNLY ETIIEADKSL 

       730        740 
PSSMATLLKN LRKKLNFKLC QAFM

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Pleiotropic nuclear defects associated with a conditional allele of the novel nucleoporin Rat9p/Nup85p." Goldstein A.L., Snay C.A., Heath C.V., Cole C.N. Mol. Biol. Cell 7:917-934(1996) [PubMed: 8816998] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN NUCLEAR MRNA EXPORT; NPC ASSEMBLY AND DISTRIBUTION. Strain: ATCC 90840 / EAY235 / FY23.
[2]	"A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores." Siniossoglou S., Wimmer C., Rieger M., Doye V., Tekotte H., Weise C., Emig S., Segref A., Hurt E.C. Cell 84:265-275(1996) [PubMed: 8565072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 31-39; 123-138 AND 708-718, NUCLEAR ENVELOPE ORGANIZATION.
[3]	"Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA genes and 14 new open reading frames including a gene most probably belonging to the family of ubiquitin-protein ligases." Huang M.-E., Chuat J.-C., Galibert F. Yeast 11:775-781(1995) [PubMed: 7668047] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	"Nuclear mRNA export requires complex formation between Mex67p and Mtr2p at the nuclear pores." Santos-Rosa H., Moreno H., Simos G., Segref A., Fahrenkrog B., Pante N., Hurt E.C. Mol. Cell. Biol. 18:6826-6838(1998) [PubMed: 9774696] [Abstract] Cited for: FUNCTION, INTERACTION WITH MTR2.
[6]	"The yeast nuclear pore complex: composition, architecture, and transport mechanism." Rout M.P., Aitchison J.D., Suprapto A., Hjertaas K., Zhao Y., Chait B.T. J. Cell Biol. 148:635-651(2000) [PubMed: 10684247] [Abstract] Cited for: CHARACTERIZATION, NPC SUBUNIT LOCATION.
[7]	"Factors affecting nuclear export of the 60S ribosomal subunit in vivo." Stage-Zimmermann T., Schmidt U., Silver P.A. Mol. Biol. Cell 11:3777-3789(2000) [PubMed: 11071906] [Abstract] Cited for: FUNCTION, PRE-RIBOSOME EXPORT.
[8]	"Deciphering networks of protein interactions at the nuclear pore complex." Allen N.P., Patel S.S., Huang L., Chalkley R.J., Burlingame A., Lutzmann M., Hurt E.C., Rexach M. Mol. Cell. Proteomics 1:930-946(2002) [PubMed: 12543930] [Abstract] Cited for: FUNCTION, INTERACTION WITH NUP116 GLFG REPEATS.
[9]	"Modular self-assembly of a Y-shaped multiprotein complex from seven nucleoporins." Lutzmann M., Kunze R., Buerer A., Aebi U., Hurt E.C. EMBO J. 21:387-397(2002) [PubMed: 11823431] [Abstract] Cited for: FUNCTION, NUP84 NPC SUBCOMPLEX ASSEMBLY/STRUCTURE.
[10]	"Nuclear accumulation of the small GTPase Gsp1p depends on nucleoporins Nup133p, Rat2p/Nup120p, Nup85p, Nic96p, and the acetyl-CoA carboxylase Acc1p." Gao H., Sumanaweera N., Bailer S.M., Stochaj U. J. Biol. Chem. 278:25331-25340(2003) [PubMed: 12730220] [Abstract] Cited for: FUNCTION, NUCLEAR GSP1 IMPORT.
[11]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[12]	"Peering through the pore: nuclear pore complex structure, assembly, and function." Suntharalingam M., Wente S.R. Dev. Cell 4:775-789(2003) [PubMed: 12791264] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

U36469 Genomic DNA. Translation: AAB48943.1.
X90995 Genomic DNA. Translation: CAA62481.1.
L36344 Genomic DNA. Translation: AAA88744.1.
Z49542 Genomic DNA. Translation: CAA89569.1.

PIR

S57061.

RefSeq

NP_012576.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3EWE	X-ray	3.50	B/D	1-564	[»]
3F3F	X-ray	2.90	C/D/G/H	1-570	[»]
3F3G	X-ray	3.75	C/D/G/H	1-570	[»]
3F3P	X-ray	3.20	C/D/G/H/K/L	1-570	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:5818N.

IntAct

P46673. 24 interactions.

STRING

P46673.

Protein family/group databases

TCDB

9.A.14.1.1. nuclear pore complex (NPC) family.

Proteomic databases

PeptideAtlas

P46673.

PRIDE

P46673.

Genome annotation databases

Ensembl

YJR042W; YJR042W; YJR042W; Saccharomyces cerevisiae. [Genome view]

GeneID

853499.

KEGG

sce:YJR042W.

NMPDR

fig|4932.3.peg.3550.

Organism-specific databases

CYGD

YJR042w.

SGD

S000003803. NUP85.

Phylogenomic databases

HOGENOM

P46673.

OMA

TNDDIEW

OrthoDB

EOG9ZCVMV

Gene expression databases

ArrayExpress

P46673.

Genevestigator

P46673.

GermOnline

YJR042W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR011502. Nucleoporin_Nup85.
[Graphical view]

Pfam

PF07575. Nucleopor_Nup85. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

974140.

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Entry information

Entry name

NUP85_YEAST

Accession

Primary (citable) accession number: P46673

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 24, 2009
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents