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Protein

Nucleoporin NUP85

Gene

NUP85

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. NUP85 is involved in nuclear poly(A)+ RNA and pre-ribosome export, in GSP1 nuclear import, in NPC assembly and distribution, as well as in nuclear envelope organization.6 Publications

GO - Molecular functioni

  • structural constituent of nuclear pore Source: SGD

GO - Biological processi

  • mRNA export from nucleus Source: SGD
  • nuclear pore distribution Source: SGD
  • positive regulation of transcription, DNA-templated Source: SGD
  • protein import into nucleus Source: SGD
  • ribosomal large subunit export from nucleus Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31677-MONOMER.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoporin NUP85
Alternative name(s):
Nuclear pore protein NUP85
Gene namesi
Name:NUP85
Synonyms:RAT9
Ordered Locus Names:YJR042W
ORF Names:J1624
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR042W.
SGDiS000003803. NUP85.

Subcellular locationi

GO - Cellular componenti

  • nuclear membrane Source: UniProtKB-SubCell
  • nuclear pore Source: SGD
  • nuclear pore outer ring Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002048851 – 744Nucleoporin NUP85Add BLAST744

Proteomic databases

MaxQBiP46673.
PRIDEiP46673.

PTM databases

iPTMnetiP46673.

Interactioni

Subunit structurei

The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEH1 and SEC1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. NUP85 is part of the heptameric 0.5 MDa autoassembling NUP84 NPC subcomplex (NUP84, NUP85, NUP120, NUP133, NUP145C, SEC13 and SEH1). NUP85 also interacts directly with the GLFG repeats of NUP116 and directly or indirectly with the mRNA transport factor MTR2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NUP100Q026294EBI-12345,EBI-11698
NUP116Q026303EBI-12345,EBI-11703
NUP120P3572910EBI-12345,EBI-11713
NUP133Q8WUM05EBI-12345,EBI-295695From a different organism.
NUP145P496878EBI-12345,EBI-11730
SEH1P530114EBI-12345,EBI-16940

Protein-protein interaction databases

BioGridi33793. 42 interactors.
DIPiDIP-5818N.
IntActiP46673. 17 interactors.
MINTiMINT-648452.

Structurei

Secondary structure

1744
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi41 – 51Combined sources11
Beta strandi67 – 74Combined sources8
Beta strandi77 – 82Combined sources6
Beta strandi89 – 91Combined sources3
Helixi102 – 117Combined sources16
Helixi119 – 122Combined sources4
Helixi135 – 164Combined sources30
Helixi170 – 189Combined sources20
Beta strandi190 – 192Combined sources3
Helixi197 – 200Combined sources4
Helixi201 – 215Combined sources15
Helixi221 – 227Combined sources7
Beta strandi237 – 240Combined sources4
Helixi243 – 254Combined sources12
Helixi258 – 265Combined sources8
Turni266 – 271Combined sources6
Helixi272 – 276Combined sources5
Helixi279 – 292Combined sources14
Helixi300 – 318Combined sources19
Helixi326 – 340Combined sources15
Helixi343 – 348Combined sources6
Helixi353 – 363Combined sources11
Helixi368 – 370Combined sources3
Helixi371 – 381Combined sources11
Beta strandi388 – 390Combined sources3
Helixi391 – 398Combined sources8
Helixi403 – 405Combined sources3
Helixi406 – 412Combined sources7
Helixi414 – 427Combined sources14
Beta strandi454 – 459Combined sources6
Helixi461 – 474Combined sources14
Turni479 – 481Combined sources3
Helixi482 – 491Combined sources10
Beta strandi493 – 495Combined sources3
Helixi497 – 507Combined sources11
Helixi508 – 510Combined sources3
Helixi516 – 529Combined sources14
Helixi532 – 543Combined sources12
Helixi545 – 548Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3EWEX-ray3.50B/D1-564[»]
3F3FX-ray2.90C/D/G/H1-570[»]
3F3GX-ray3.75C/D/G/H1-570[»]
3F3PX-ray3.20C/D/G/H/K/L1-570[»]
4XMMX-ray7.38D44-744[»]
4XMNX-ray7.60D73-743[»]
ProteinModelPortaliP46673.
SMRiP46673.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP46673.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi646 – 652Poly-Leu7

Sequence similaritiesi

Belongs to the nucleoporin Nup85 family.Curated

Phylogenomic databases

HOGENOMiHOG000113893.
InParanoidiP46673.
KOiK14304.
OMAiTNDDIEW.
OrthoDBiEOG092C2XAD.

Family and domain databases

InterProiIPR011502. Nucleoporin_Nup85.
[Graphical view]
PfamiPF07575. Nucleopor_Nup85. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46673-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIDDSNRLL MDVDQFDFLD DGTAQLSNNK TDEEEQLYKR DPVSGAILVP
60 70 80 90 100
MTVNDQPIEK NGDKMPLKFK LGPLSYQNMA FITAKDKYKL YPVRIPRLDT
110 120 130 140 150
SKEFSAYVSG LFEIYRDLGD DRVFNVPTIG VVNSNFAKEH NATVNLAMEA
160 170 180 190 200
ILNELEVFIG RVKDQDGRVN RFYELEESLT VLNCLRTMYF ILDGQDVEEN
210 220 230 240 250
RSEFIESLLN WINRSDGEPD EEYIEQVFSV KDSTAGKKVF ETQYFWKLLN
260 270 280 290 300
QLVLRGLLSQ AIGCIERSDL LPYLSDTCAV SFDAVSDSIE LLKQYPKDSS
310 320 330 340 350
STFREWKNLV LKLSQAFGSS ATDISGELRD YIEDFLLVIG GNQRKILQYS
360 370 380 390 400
RTWYESFCGF LLYYIPSLEL SAEYLQMSLE ANVVDITNDW EQPCVDIISG
410 420 430 440 450
KIHSILPVME SLDSCTAAFT AMICEAKGLI ENIFEGEKNS DDYSNEDNEM
460 470 480 490 500
LEDLFSYRNG MASYMLNSFA FELCSLGDKE LWPVAIGLIA LSATGTRSAK
510 520 530 540 550
KMVIAELLPH YPFVTNDDIE WMLSICVEWR LPEIAKEIYT TLGNQMLSAH
560 570 580 590 600
NIIESIANFS RAGKYELVKS YSWLLFEASC MEGQKLDDPV LNAIVSKNSP
610 620 630 640 650
AEDDVIIPQD ILDCVVTNSM RQTLAPYAVL SQFYELRDRE DWGQALRLLL
660 670 680 690 700
LLIEFPYLPK HYLVLLVAKF LYPIFLLDDK KLMDEDSVAT VIEVIETKWD
710 720 730 740
DADEKSSNLY ETIIEADKSL PSSMATLLKN LRKKLNFKLC QAFM
Length:744
Mass (Da):84,898
Last modified:November 1, 1995 - v1
Checksum:i4C0A4AD3DB57A023
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti101S → T in AAB48943 (PubMed:8816998).Curated1
Sequence conflicti471F → L in AAB48943 (PubMed:8816998).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36469 Genomic DNA. Translation: AAB48943.1.
X90995 Genomic DNA. Translation: CAA62481.1.
L36344 Genomic DNA. Translation: AAA88744.1.
Z49542 Genomic DNA. Translation: CAA89569.1.
BK006943 Genomic DNA. Translation: DAA08829.1.
PIRiS57061.
RefSeqiNP_012576.1. NM_001181700.1.

Genome annotation databases

EnsemblFungiiYJR042W; YJR042W; YJR042W.
GeneIDi853499.
KEGGisce:YJR042W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36469 Genomic DNA. Translation: AAB48943.1.
X90995 Genomic DNA. Translation: CAA62481.1.
L36344 Genomic DNA. Translation: AAA88744.1.
Z49542 Genomic DNA. Translation: CAA89569.1.
BK006943 Genomic DNA. Translation: DAA08829.1.
PIRiS57061.
RefSeqiNP_012576.1. NM_001181700.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3EWEX-ray3.50B/D1-564[»]
3F3FX-ray2.90C/D/G/H1-570[»]
3F3GX-ray3.75C/D/G/H1-570[»]
3F3PX-ray3.20C/D/G/H/K/L1-570[»]
4XMMX-ray7.38D44-744[»]
4XMNX-ray7.60D73-743[»]
ProteinModelPortaliP46673.
SMRiP46673.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33793. 42 interactors.
DIPiDIP-5818N.
IntActiP46673. 17 interactors.
MINTiMINT-648452.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

PTM databases

iPTMnetiP46673.

Proteomic databases

MaxQBiP46673.
PRIDEiP46673.

Protocols and materials databases

DNASUi853499.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR042W; YJR042W; YJR042W.
GeneIDi853499.
KEGGisce:YJR042W.

Organism-specific databases

EuPathDBiFungiDB:YJR042W.
SGDiS000003803. NUP85.

Phylogenomic databases

HOGENOMiHOG000113893.
InParanoidiP46673.
KOiK14304.
OMAiTNDDIEW.
OrthoDBiEOG092C2XAD.

Enzyme and pathway databases

BioCyciYEAST:G3O-31677-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP46673.
PROiP46673.

Family and domain databases

InterProiIPR011502. Nucleoporin_Nup85.
[Graphical view]
PfamiPF07575. Nucleopor_Nup85. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUP85_YEAST
AccessioniPrimary (citable) accession number: P46673
Secondary accession number(s): D6VWL3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7920 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.