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Protein

tRNA-aminoacylation cofactor ARC1

Gene

ARC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to tRNA and functions as a cofactor for the methionyl-tRNA synthetase (MetRS) and glutamyl-tRNA synthetase (GluRS). Forms a complex with MetRS and GluRS and increases their affinity for cognate tRNAs due to the presence of a tRNA binding domain in its middle and C-terminal part. Binds specifically G4 quadruplex nucleic acid structures (these are four-stranded right-handed helices, stabilized by guanine base quartets). Also required for cytoplasmic confinement of the synthetases and tRNA.4 Publications

GO - Molecular functioni

  • enzyme activator activity Source: SGD
  • phosphatidylinositol-3,5-bisphosphate binding Source: SGD
  • phosphatidylinositol-3-phosphate binding Source: SGD
  • tRNA binding Source: SGD

GO - Biological processi

  • positive regulation of ligase activity Source: SGD
  • tRNA aminoacylation for protein translation Source: SGD
Complete GO annotation...

Keywords - Ligandi

RNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30604-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-aminoacylation cofactor ARC1
Alternative name(s):
Acyl-RNA-complex protein 1
GU4 nucleic-binding protein 1
Short name:
G4p1 protein
P42
tRNA-interacting factor ARC1
Gene namesi
Name:ARC1
Synonyms:G4P1
Ordered Locus Names:YGL105W
ORF Names:G3085
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL105W.
SGDiS000003073. ARC1.

Subcellular locationi

GO - Cellular componenti

  • aminoacyl-tRNA synthetase multienzyme complex Source: GO_Central
  • cytoplasm Source: SGD
  • cytoplasmic stress granule Source: SGD
  • methionyl glutamyl tRNA synthetase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi26A → R: Abolishes interaction with MES1. 1 Publication1
Mutagenesisi100R → A: Abolishes interaction with GUS1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000874091 – 376tRNA-aminoacylation cofactor ARC1Add BLAST376

Proteomic databases

MaxQBiP46672.
PRIDEiP46672.
TopDownProteomicsiP46672.

Interactioni

Subunit structurei

Component of a yeast aminoacyl-tRNA synthase (aaRS) complex formed by methionyl-tRNA synthase MES1, glutamyl-tRNA synthase GUS1 and the tRNA aminoacylation cofactor ARC1 in a stoichiometric complex. Interacts (via N-ter) with MES1 (via N-ter) and GUS1 (via N-ter). Can also form a stable binary complex with either MES1 or GUS1 that is functional in terms of aminoacylation.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GUS1P466557EBI-7224,EBI-18665
MES1P009587EBI-7224,EBI-18762

Protein-protein interaction databases

BioGridi33145. 183 interactors.
DIPiDIP-2210N.
IntActiP46672. 7 interactors.
MINTiMINT-705320.

Structurei

Secondary structure

1376
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 9Combined sources7
Helixi12 – 15Combined sources4
Helixi22 – 36Combined sources15
Helixi41 – 43Combined sources3
Helixi44 – 53Combined sources10
Helixi65 – 83Combined sources19
Helixi88 – 94Combined sources7
Helixi96 – 108Combined sources13
Turni113 – 115Combined sources3
Helixi206 – 208Combined sources3
Beta strandi211 – 221Combined sources11
Beta strandi229 – 234Combined sources6
Beta strandi242 – 247Combined sources6
Turni249 – 251Combined sources3
Helixi254 – 257Combined sources4
Beta strandi261 – 265Combined sources5
Beta strandi271 – 273Combined sources3
Beta strandi276 – 278Combined sources3
Beta strandi284 – 286Combined sources3
Beta strandi291 – 293Combined sources3
Beta strandi305 – 308Combined sources4
Helixi322 – 324Combined sources3
Helixi326 – 330Combined sources5
Helixi331 – 333Combined sources3
Beta strandi334 – 336Combined sources3
Beta strandi341 – 345Combined sources5
Beta strandi354 – 359Combined sources6
Beta strandi374 – 376Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HQTX-ray1.90A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T1-122[»]
2HRKX-ray2.05B1-122[»]
2HSMX-ray3.00B1-122[»]
2HSNX-ray2.20B1-122[»]
4R1JX-ray1.40A201-376[»]
ProteinModelPortaliP46672.
SMRiP46672.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP46672.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini205 – 307tRNA-bindingPROSITE-ProRule annotationAdd BLAST103

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni22 – 46Interaction with methionyl-tRNA synthetase MES1Add BLAST25
Regioni52 – 61Interaction with glutamyl-tRNA synthetase GUS110
Regioni91 – 121Interaction with glutamyl-tRNA synthetase GUS1Add BLAST31

Sequence similaritiesi

Contains 1 tRNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00790000123080.
HOGENOMiHOG000167473.
InParanoidiP46672.
KOiK15437.
OMAiVIDFRVG.
OrthoDBiEOG092C3R7L.

Family and domain databases

InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR012340. NA-bd_OB-fold.
IPR002547. tRNA-bd_dom.
[Graphical view]
PfamiPF01588. tRNA_bind. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF50249. SSF50249. 1 hit.
PROSITEiPS50886. TRBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46672-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDLVTKFES LIISKYPVSF TKEQSAQAAQ WESVLKSGQI QPHLDQLNLV
60 70 80 90 100
LRDNTFIVST LYPTSTDVHV FEVALPLIKD LVASSKDVKS TYTTYRHILR
110 120 130 140 150
WIDYMQNLLE VSSTDKLEIN HDLDLPHEVI EKKKKAPAGG AADAAAKADE
160 170 180 190 200
DVSKKAKKQD HPRGKPDEET LKKLREEAKA KKAAKKAANA KQQQEQQNKA
210 220 230 240 250
PEKPKPSAID FRVGFIQKAI KHPDADSLYV STIDVGDEEG PRTVCSGLVK
260 270 280 290 300
HFPLDAMQER YVVVVCNLKP VNMRGIKSTA MVLCGSNDDK VEFVEPPKDS
310 320 330 340 350
KAGDKVFFEG FGDEAPMKQL NPKKKIWEHL QPHFTTNDGL EVIFKDEEEK
360 370
DHPVRKLTNA KGESFKVASI ANAQVR
Length:376
Mass (Da):42,084
Last modified:October 1, 1996 - v2
Checksum:iB0CD64AD564900C7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti137 – 149PAGGA…AAKAD → LRVALLMLQQGS in AAC49072 (PubMed:7657649).CuratedAdd BLAST13
Sequence conflicti181 – 182KK → LL in AAC49072 (PubMed:7657649).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31348 Genomic DNA. Translation: AAC49072.1.
X97644 Genomic DNA. Translation: CAA66247.1.
Z72627 Genomic DNA. Translation: CAA96812.1.
X95481 Genomic DNA. Translation: CAA64750.1.
AY558498 Genomic DNA. Translation: AAS56824.1.
BK006941 Genomic DNA. Translation: DAA08002.1.
PIRiS64113.
RefSeqiNP_011410.1. NM_001180970.1.

Genome annotation databases

EnsemblFungiiYGL105W; YGL105W; YGL105W.
GeneIDi852773.
KEGGisce:YGL105W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31348 Genomic DNA. Translation: AAC49072.1.
X97644 Genomic DNA. Translation: CAA66247.1.
Z72627 Genomic DNA. Translation: CAA96812.1.
X95481 Genomic DNA. Translation: CAA64750.1.
AY558498 Genomic DNA. Translation: AAS56824.1.
BK006941 Genomic DNA. Translation: DAA08002.1.
PIRiS64113.
RefSeqiNP_011410.1. NM_001180970.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HQTX-ray1.90A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T1-122[»]
2HRKX-ray2.05B1-122[»]
2HSMX-ray3.00B1-122[»]
2HSNX-ray2.20B1-122[»]
4R1JX-ray1.40A201-376[»]
ProteinModelPortaliP46672.
SMRiP46672.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33145. 183 interactors.
DIPiDIP-2210N.
IntActiP46672. 7 interactors.
MINTiMINT-705320.

Proteomic databases

MaxQBiP46672.
PRIDEiP46672.
TopDownProteomicsiP46672.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL105W; YGL105W; YGL105W.
GeneIDi852773.
KEGGisce:YGL105W.

Organism-specific databases

EuPathDBiFungiDB:YGL105W.
SGDiS000003073. ARC1.

Phylogenomic databases

GeneTreeiENSGT00790000123080.
HOGENOMiHOG000167473.
InParanoidiP46672.
KOiK15437.
OMAiVIDFRVG.
OrthoDBiEOG092C3R7L.

Enzyme and pathway databases

BioCyciYEAST:G3O-30604-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP46672.
PROiP46672.

Family and domain databases

InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR012340. NA-bd_OB-fold.
IPR002547. tRNA-bd_dom.
[Graphical view]
PfamiPF01588. tRNA_bind. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF50249. SSF50249. 1 hit.
PROSITEiPS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARC1_YEAST
AccessioniPrimary (citable) accession number: P46672
Secondary accession number(s): D6VU41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 154 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 57700 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.