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Protein

Adenylosuccinate synthetase isozyme 2

Gene

Adss

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.1 Publication

Catalytic activityi

GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Enzyme regulationi

Inhibited competitively by AMP and IMP and non-competitively by fructose 1,6-bisphosphate.

Kineticsi

  1. KM=15 µM for GTP1 Publication
  2. KM=12 µM for IMP1 Publication
  3. KM=950 µM for L-aspartate1 Publication

    pH dependencei

    Optimum pH is 6.6-6.9.1 Publication

    Pathwayi: AMP biosynthesis via de novo pathway

    This protein is involved in step 1 of the subpathway that synthesizes AMP from IMP.UniRule annotation
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. Adenylosuccinate synthetase isozyme 1 (Adssl1), Adenylosuccinate synthetase isozyme 1 (Adssl1), Adenylosuccinate synthetase (Adss), Adenylosuccinate synthetase isozyme 2 (Adss), Adenylosuccinate synthetase isozyme 2 (Adss), Adenylosuccinate synthetase isozyme 1 (Adssl1)
    2. Adenylosuccinate lyase (Adsl), Adenylosuccinate lyase (Adsl), Adenylosuccinate lyase (Adsl)
    This subpathway is part of the pathway AMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from IMP, the pathway AMP biosynthesis via de novo pathway and in Purine metabolism.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei40 – 401Proton acceptorUniRule annotation
    Metal bindingi40 – 401MagnesiumUniRule annotation
    Binding sitei40 – 401SubstrateUniRule annotation
    Metal bindingi67 – 671Magnesium; via carbonyl oxygenUniRule annotation
    Active sitei68 – 681Proton donorUniRule annotation
    Binding sitei162 – 1621IMPUniRule annotation
    Binding sitei176 – 1761IMP; shared with dimeric partnerUniRule annotation
    Binding sitei255 – 2551IMPUniRule annotation
    Binding sitei270 – 2701IMPUniRule annotation
    Binding sitei334 – 3341IMPUniRule annotation
    Binding sitei336 – 3361GTPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi39 – 457GTPUniRule annotation
    Nucleotide bindingi67 – 693GTPUniRule annotation
    Nucleotide bindingi362 – 3643GTPUniRule annotation
    Nucleotide bindingi444 – 4474GTPUniRule annotation

    GO - Molecular functioni

    • adenylosuccinate synthase activity Source: MGI
    • GTP binding Source: UniProtKB-HAMAP
    • magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiR-MMU-73817. Purine ribonucleoside monophosphate biosynthesis.
    UniPathwayiUPA00075; UER00335.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenylosuccinate synthetase isozyme 2UniRule annotation (EC:6.3.4.4UniRule annotation)
    Short name:
    AMPSase 2UniRule annotation
    Short name:
    AdSS 2UniRule annotation
    Alternative name(s):
    Adenylosuccinate synthetase, acidic isozymeUniRule annotation
    Adenylosuccinate synthetase, liver isozymeUniRule annotation
    Short name:
    L-type adenylosuccinate synthetaseUniRule annotation
    IMP--aspartate ligase 2UniRule annotation
    Gene namesi
    Name:Adss
    Synonyms:Adss2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 1

    Organism-specific databases

    MGIiMGI:87948. Adss.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 456456Adenylosuccinate synthetase isozyme 2PRO_0000095131Add
    BLAST

    Proteomic databases

    EPDiP46664.
    MaxQBiP46664.
    PaxDbiP46664.
    PRIDEiP46664.

    2D gel databases

    REPRODUCTION-2DPAGEP46664.
    Q9CQL9.

    PTM databases

    iPTMnetiP46664.
    PhosphoSiteiP46664.

    Expressioni

    Gene expression databases

    BgeeiENSMUSG00000015961.
    CleanExiMM_ADSS.
    ExpressionAtlasiP46664. baseline and differential.
    GenevisibleiP46664. MM.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    IntActiP46664. 1 interaction.
    MINTiMINT-4124938.
    STRINGi10090.ENSMUSP00000016105.

    Structurei

    3D structure databases

    ProteinModelPortaliP46664.
    SMRiP46664. Positions 26-456.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni40 – 434IMP bindingUniRule annotation
    Regioni65 – 684IMP bindingUniRule annotation
    Regioni330 – 3367Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the adenylosuccinate synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiKOG1355. Eukaryota.
    COG0104. LUCA.
    GeneTreeiENSGT00390000015553.
    HOGENOMiHOG000260959.
    HOVERGENiHBG053768.
    InParanoidiP46664.
    KOiK01939.
    OMAiYAIEISG.
    OrthoDBiEOG091G1BIK.
    PhylomeDBiP46664.
    TreeFamiTF300486.

    Family and domain databases

    HAMAPiMF_00011. Adenylosucc_synth. 1 hit.
    MF_03127. Adenylosucc_synth_vert_acid. 1 hit.
    InterProiIPR018220. Adenylosuccin_syn_GTP-bd.
    IPR033128. Adenylosuccin_syn_Lys_AS.
    IPR001114. Adenylosuccinate_synthetase.
    IPR027529. AdSS_2_vert.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR11846. PTHR11846. 1 hit.
    PfamiPF00709. Adenylsucc_synt. 1 hit.
    [Graphical view]
    SMARTiSM00788. Adenylsucc_synt. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00184. purA. 1 hit.
    PROSITEiPS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
    PS00513. ADENYLOSUCCIN_SYN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P46664-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSISESSPAA TSLPNGDCGR PRARSGGNRV TVVLGAQWGD EGKGKVVDLL
    60 70 80 90 100
    AQDADIVCRC QGGNNAGHTV VVDSVEYDFH LLPSGIINPN VTAFIGNGVV
    110 120 130 140 150
    IHLPGLFEEA EKNVQKGKGL DGWEKRLIIS DRAHIVFDFH QAADGIQEQQ
    160 170 180 190 200
    RQEQAGKNLG TTKKGIGPVY SSKAARSGLR MCDLVSDFDG FSERFKVLAN
    210 220 230 240 250
    QYKSIYPTLE IDIEGELQQL KGYMERIKPM VKDGVYFLYE ALHGPPKKIL
    260 270 280 290 300
    VEGANAALLD IDFGTYPFVT SSNCTVGGVC TGLGMPPQNV GEVYGVVKAY
    310 320 330 340 350
    TTRVGIGAFP TEQDNEIGEL LQTRGREFGV TTGRKRRCGW LDLVSLKYAH
    360 370 380 390 400
    MINGFTALAL TKLDILDMFT EIKVGVAYKL DGETIPHFPA NQEVLNKVEV
    410 420 430 440 450
    QYKTLPGWNT DISNARTFKE LPVNAQNYVR FIEDELQIPV KWIGVGKSRE

    SMIQLF
    Length:456
    Mass (Da):50,021
    Last modified:March 7, 2006 - v2
    Checksum:i7825B758BC6CDA38
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti167 – 1671G → R in AAA19727 (PubMed:8308018).Curated
    Sequence conflicti199 – 1991A → T in AAA19727 (PubMed:8308018).Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L24554 mRNA. Translation: AAA19727.1.
    AK004877 mRNA. Translation: BAB23635.1.
    AK010263 mRNA. Translation: BAB26805.1.
    AK028060 mRNA. Translation: BAC25730.1.
    AK148420 mRNA. Translation: BAE28543.1.
    AK170279 mRNA. Translation: BAE41682.1.
    CCDSiCCDS15555.1.
    PIRiA53162.
    RefSeqiNP_031448.2. NM_007422.3.
    UniGeneiMm.338021.

    Genome annotation databases

    EnsembliENSMUST00000016105; ENSMUSP00000016105; ENSMUSG00000015961.
    GeneIDi11566.
    KEGGimmu:11566.
    UCSCiuc007dut.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L24554 mRNA. Translation: AAA19727.1.
    AK004877 mRNA. Translation: BAB23635.1.
    AK010263 mRNA. Translation: BAB26805.1.
    AK028060 mRNA. Translation: BAC25730.1.
    AK148420 mRNA. Translation: BAE28543.1.
    AK170279 mRNA. Translation: BAE41682.1.
    CCDSiCCDS15555.1.
    PIRiA53162.
    RefSeqiNP_031448.2. NM_007422.3.
    UniGeneiMm.338021.

    3D structure databases

    ProteinModelPortaliP46664.
    SMRiP46664. Positions 26-456.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    IntActiP46664. 1 interaction.
    MINTiMINT-4124938.
    STRINGi10090.ENSMUSP00000016105.

    PTM databases

    iPTMnetiP46664.
    PhosphoSiteiP46664.

    2D gel databases

    REPRODUCTION-2DPAGEP46664.
    Q9CQL9.

    Proteomic databases

    EPDiP46664.
    MaxQBiP46664.
    PaxDbiP46664.
    PRIDEiP46664.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000016105; ENSMUSP00000016105; ENSMUSG00000015961.
    GeneIDi11566.
    KEGGimmu:11566.
    UCSCiuc007dut.1. mouse.

    Organism-specific databases

    CTDi159.
    MGIiMGI:87948. Adss.

    Phylogenomic databases

    eggNOGiKOG1355. Eukaryota.
    COG0104. LUCA.
    GeneTreeiENSGT00390000015553.
    HOGENOMiHOG000260959.
    HOVERGENiHBG053768.
    InParanoidiP46664.
    KOiK01939.
    OMAiYAIEISG.
    OrthoDBiEOG091G1BIK.
    PhylomeDBiP46664.
    TreeFamiTF300486.

    Enzyme and pathway databases

    UniPathwayiUPA00075; UER00335.
    ReactomeiR-MMU-73817. Purine ribonucleoside monophosphate biosynthesis.

    Miscellaneous databases

    PROiP46664.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSMUSG00000015961.
    CleanExiMM_ADSS.
    ExpressionAtlasiP46664. baseline and differential.
    GenevisibleiP46664. MM.

    Family and domain databases

    HAMAPiMF_00011. Adenylosucc_synth. 1 hit.
    MF_03127. Adenylosucc_synth_vert_acid. 1 hit.
    InterProiIPR018220. Adenylosuccin_syn_GTP-bd.
    IPR033128. Adenylosuccin_syn_Lys_AS.
    IPR001114. Adenylosuccinate_synthetase.
    IPR027529. AdSS_2_vert.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR11846. PTHR11846. 1 hit.
    PfamiPF00709. Adenylsucc_synt. 1 hit.
    [Graphical view]
    SMARTiSM00788. Adenylsucc_synt. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00184. purA. 1 hit.
    PROSITEiPS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
    PS00513. ADENYLOSUCCIN_SYN_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPURA2_MOUSE
    AccessioniPrimary (citable) accession number: P46664
    Secondary accession number(s): Q9CQL9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: March 7, 2006
    Last modified: September 7, 2016
    This is version 144 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.