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Protein

Alpha-internexin

Gene

Ina

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • intermediate filament cytoskeleton organization Source: MGI
  • neurofilament cytoskeleton organization Source: MGI
  • substantia nigra development Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-internexin
Short name:
Alpha-Inx
Alternative name(s):
66 kDa neurofilament protein
Short name:
NF-66
Short name:
Neurofilament-66
Gene namesi
Name:Ina
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:96568. Ina.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic ribonucleoprotein granule Source: MGI
  • extracellular space Source: MGI
  • intermediate filament cytoskeleton Source: MGI
  • myelin sheath Source: UniProtKB
  • neurofilament Source: MGI
  • nuclear membrane Source: MGI
  • nucleoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Intermediate filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 501501Alpha-internexinPRO_0000063784Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei72 – 721PhosphoserineCombined sources
Modified residuei219 – 2191PhosphoserineCombined sources
Modified residuei290 – 2901N6-acetyllysineBy similarity
Modified residuei335 – 3351PhosphoserineCombined sources
Modified residuei498 – 4981PhosphoserineBy similarity

Post-translational modificationi

O-glycosylated.1 Publication

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP46660.
MaxQBiP46660.
PaxDbiP46660.
PRIDEiP46660.

2D gel databases

UCD-2DPAGEP46660.

PTM databases

iPTMnetiP46660.
PhosphoSiteiP46660.
SwissPalmiP46660.

Expressioni

Gene expression databases

BgeeiP46660.
GenevisibleiP46660. MM.

Interactioni

Protein-protein interaction databases

BioGridi230487. 5 interactions.
IntActiP46660. 9 interactions.
MINTiMINT-136763.
STRINGi10090.ENSMUSP00000041347.

Structurei

3D structure databases

ProteinModelPortaliP46660.
SMRiP46660. Positions 91-231, 259-329, 333-402.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 8787HeadAdd
BLAST
Regioni88 – 408321RodAdd
BLAST
Regioni88 – 12942Coil 1AAdd
BLAST
Regioni130 – 14213Linker 1Add
BLAST
Regioni143 – 23896Coil 1BAdd
BLAST
Regioni239 – 26224Linker 2Add
BLAST
Regioni263 – 408146Coil 2Add
BLAST
Regioni409 – 50193TailAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi449 – 4568Poly-Glu

Sequence similaritiesi

Belongs to the intermediate filament family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IGME. Eukaryota.
ENOG410XPTM. LUCA.
GeneTreeiENSGT00830000128228.
HOGENOMiHOG000230977.
HOVERGENiHBG013015.
InParanoidiP46660.
KOiK07608.
OMAiASSYRKV.
OrthoDBiEOG7FV3Q8.
TreeFamiTF330122.

Family and domain databases

InterProiIPR027703. Alpha-Inx.
IPR001664. IF.
IPR006821. Intermed_filament_DNA-bd.
IPR018039. Intermediate_filament_CS.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PTHR23239:SF132. PTHR23239:SF132. 1 hit.
PfamiPF00038. Filament. 1 hit.
PF04732. Filament_head. 1 hit.
[Graphical view]
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46660-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFGSEHYLC SASSYRKVFG DSSRLSARLS GPGGSGSFRS QSLSRSNVAS
60 70 80 90 100
TAACSSASSL GLGLAYRRLP ASDGLDLSQA AARTNEYKII RTNEKEQLQG
110 120 130 140 150
LNDRFAVFIE KVHQLETQNR ALEAELAALR QRHAEPSRVG ELFQRELREL
160 170 180 190 200
RAQLEEASSA RAQALLERDG LAEEVQRLRA RCEEESRGRE GAERALKAQQ
210 220 230 240 250
RDVDGATLAR LDLEKKVESL LDELAFVRQV HDEEVAELLA TLQASSQAAA
260 270 280 290 300
EVDVAVAKPD LTSALREIRA QYESLAAKNL QSAEEWYKSK FANLNEQAAR
310 320 330 340 350
STEAIRASRE EIHEYRRQLQ ARTIEIEGLR GANESLERQI LELEERHSAE
360 370 380 390 400
VAGYQDSIGQ LESDLRNTKS EMARHLREYQ DLLNVKMALD IEIAAYRKLL
410 420 430 440 450
EGEETRFSTG GLSISGLNPL PNPSYLLPPR ILSSTASKVS SAGLSLKKEE
460 470 480 490 500
EEEEEEASKE VSKKTSKVGE GFEETLGEAV ISTKKTGKSA TEESTSSSQK

M
Length:501
Mass (Da):55,383
Last modified:January 22, 2014 - v3
Checksum:iB3DEF649DEB29C3B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 281R → P in AAB37740 (PubMed:8734438).Curated
Sequence conflicti34 – 341G → A in AAB37740 (PubMed:8734438).Curated
Sequence conflicti141 – 1411E → Q in AAB37740 (PubMed:8734438).Curated
Sequence conflicti147 – 1471L → R in AAB37740 (PubMed:8734438).Curated
Sequence conflicti163 – 1631Q → E in AAA62617 (PubMed:7959004).Curated
Sequence conflicti297 – 2971Q → L in AAB37740 (PubMed:8734438).Curated
Sequence conflicti301 – 3011S → T in AAB37740 (PubMed:8734438).Curated
Sequence conflicti318 – 3192QL → HV in AAB37740 (PubMed:8734438).Curated
Sequence conflicti347 – 3471H → D in AAA62617 (PubMed:7959004).Curated
Sequence conflicti383 – 3831L → F in AAA62617 (PubMed:7959004).Curated
Sequence conflicti388 – 3881A → G in AAA62617 (PubMed:7959004).Curated
Sequence conflicti403 – 4031E → N in AAB37740 (PubMed:8734438).Curated
Sequence conflicti442 – 4421A → T in AAA62617 (PubMed:7959004).Curated
Sequence conflicti442 – 4421A → T in AAB37740 (PubMed:8734438).Curated
Sequence conflicti443 – 4431G → R in AAA62617 (PubMed:7959004).Curated
Sequence conflicti450 – 4501E → D in AAA62617 (PubMed:7959004).Curated
Sequence conflicti450 – 4501E → D in AAB37740 (PubMed:8734438).Curated
Sequence conflicti455 – 4551E → EEEE in AAA62617 (PubMed:7959004).Curated
Sequence conflicti456 – 4561E → D in AAA62617 (PubMed:7959004).Curated
Sequence conflicti456 – 4561E → D in AAB37740 (PubMed:8734438).Curated
Sequence conflicti489 – 4891S → L in AAB37740 (PubMed:8734438).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27220 Genomic DNA. Translation: AAA62617.1.
L36390 mRNA. Translation: AAB37740.1.
BC018383 mRNA. Translation: AAH18383.1.
CCDSiCCDS38013.1.
PIRiI53868.
RefSeqiNP_666212.3. NM_146100.4.
UniGeneiMm.276251.

Genome annotation databases

EnsembliENSMUST00000037636; ENSMUSP00000041347; ENSMUSG00000034336.
GeneIDi226180.
KEGGimmu:226180.
UCSCiuc008huk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27220 Genomic DNA. Translation: AAA62617.1.
L36390 mRNA. Translation: AAB37740.1.
BC018383 mRNA. Translation: AAH18383.1.
CCDSiCCDS38013.1.
PIRiI53868.
RefSeqiNP_666212.3. NM_146100.4.
UniGeneiMm.276251.

3D structure databases

ProteinModelPortaliP46660.
SMRiP46660. Positions 91-231, 259-329, 333-402.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230487. 5 interactions.
IntActiP46660. 9 interactions.
MINTiMINT-136763.
STRINGi10090.ENSMUSP00000041347.

PTM databases

iPTMnetiP46660.
PhosphoSiteiP46660.
SwissPalmiP46660.

2D gel databases

UCD-2DPAGEP46660.

Proteomic databases

EPDiP46660.
MaxQBiP46660.
PaxDbiP46660.
PRIDEiP46660.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037636; ENSMUSP00000041347; ENSMUSG00000034336.
GeneIDi226180.
KEGGimmu:226180.
UCSCiuc008huk.1. mouse.

Organism-specific databases

CTDi9118.
MGIiMGI:96568. Ina.

Phylogenomic databases

eggNOGiENOG410IGME. Eukaryota.
ENOG410XPTM. LUCA.
GeneTreeiENSGT00830000128228.
HOGENOMiHOG000230977.
HOVERGENiHBG013015.
InParanoidiP46660.
KOiK07608.
OMAiASSYRKV.
OrthoDBiEOG7FV3Q8.
TreeFamiTF330122.

Miscellaneous databases

ChiTaRSiIna. mouse.
PROiP46660.
SOURCEiSearch...

Gene expression databases

BgeeiP46660.
GenevisibleiP46660. MM.

Family and domain databases

InterProiIPR027703. Alpha-Inx.
IPR001664. IF.
IPR006821. Intermed_filament_DNA-bd.
IPR018039. Intermediate_filament_CS.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PTHR23239:SF132. PTHR23239:SF132. 1 hit.
PfamiPF00038. Filament. 1 hit.
PF04732. Filament_head. 1 hit.
[Graphical view]
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the mouse gene encoding the neuronal intermediate filament protein alpha-internexin."
    Chien C.-L., Liem R.K.H.
    Gene 149:289-292(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: C57BL/6 X CBA.
    Tissue: Spleen.
  2. "The 66-kDa neurofilament protein (NF-66): sequence analysis and evolution."
    Chan S.O., Chiu F.C.
    Neurochem. Res. 21:449-455(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  4. Lubec G., Klug S., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 17-24; 29-39; 46-83; 92-130; 139-145; 152-177; 202-210; 216-228; 270-288; 291-300; 310-316; 323-366; 378-430; 431-438 AND 471-487, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  5. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-72 AND SER-335, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  6. "O-linked N-acetylglucosamine proteomics of postsynaptic density preparations using lectin weak affinity chromatography and mass spectrometry."
    Vosseller K., Trinidad J.C., Chalkley R.J., Specht C.G., Thalhammer A., Lynn A.J., Snedecor J.O., Guan S., Medzihradszky K.F., Maltby D.A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:923-934(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-219, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiAINX_MOUSE
AccessioniPrimary (citable) accession number: P46660
Secondary accession number(s): Q61958, Q8VCW5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 22, 2014
Last modified: June 8, 2016
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.