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Protein

Alpha-internexin

Gene

Ina

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • intermediate filament cytoskeleton organization Source: MGI
  • neurofilament cytoskeleton organization Source: MGI
  • substantia nigra development Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-internexin
Short name:
Alpha-Inx
Alternative name(s):
66 kDa neurofilament protein
Short name:
NF-66
Short name:
Neurofilament-66
Gene namesi
Name:Ina
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:96568. Ina.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic ribonucleoprotein granule Source: MGI
  • extracellular space Source: MGI
  • intermediate filament cytoskeleton Source: MGI
  • myelin sheath Source: UniProtKB
  • neurofilament Source: MGI
  • nuclear membrane Source: MGI
  • nucleoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Intermediate filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000637841 – 501Alpha-internexinAdd BLAST501

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei72PhosphoserineCombined sources1
Modified residuei219PhosphoserineCombined sources1
Modified residuei290N6-acetyllysineBy similarity1
Modified residuei335PhosphoserineCombined sources1
Modified residuei498PhosphoserineBy similarity1

Post-translational modificationi

O-glycosylated.1 Publication

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP46660.
PaxDbiP46660.
PeptideAtlasiP46660.
PRIDEiP46660.

2D gel databases

UCD-2DPAGEP46660.

PTM databases

iPTMnetiP46660.
PhosphoSitePlusiP46660.
SwissPalmiP46660.

Expressioni

Gene expression databases

BgeeiENSMUSG00000034336.
GenevisibleiP46660. MM.

Interactioni

Protein-protein interaction databases

BioGridi230487. 5 interactors.
IntActiP46660. 9 interactors.
MINTiMINT-136763.
STRINGi10090.ENSMUSP00000041347.

Structurei

3D structure databases

ProteinModelPortaliP46660.
SMRiP46660.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 87HeadAdd BLAST87
Regioni88 – 408RodAdd BLAST321
Regioni88 – 129Coil 1AAdd BLAST42
Regioni130 – 142Linker 1Add BLAST13
Regioni143 – 238Coil 1BAdd BLAST96
Regioni239 – 262Linker 2Add BLAST24
Regioni263 – 408Coil 2Add BLAST146
Regioni409 – 501TailAdd BLAST93

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi449 – 456Poly-Glu8

Sequence similaritiesi

Belongs to the intermediate filament family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IGME. Eukaryota.
ENOG410XPTM. LUCA.
GeneTreeiENSGT00830000128228.
HOGENOMiHOG000230977.
HOVERGENiHBG013015.
InParanoidiP46660.
KOiK07608.
OMAiASSYRKV.
OrthoDBiEOG091G12MK.
TreeFamiTF330122.

Family and domain databases

InterProiIPR027703. Alpha-Inx.
IPR001664. IF.
IPR006821. Intermed_filament_DNA-bd.
IPR018039. Intermediate_filament_CS.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PTHR23239:SF132. PTHR23239:SF132. 1 hit.
PfamiPF00038. Filament. 1 hit.
PF04732. Filament_head. 1 hit.
[Graphical view]
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46660-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFGSEHYLC SASSYRKVFG DSSRLSARLS GPGGSGSFRS QSLSRSNVAS
60 70 80 90 100
TAACSSASSL GLGLAYRRLP ASDGLDLSQA AARTNEYKII RTNEKEQLQG
110 120 130 140 150
LNDRFAVFIE KVHQLETQNR ALEAELAALR QRHAEPSRVG ELFQRELREL
160 170 180 190 200
RAQLEEASSA RAQALLERDG LAEEVQRLRA RCEEESRGRE GAERALKAQQ
210 220 230 240 250
RDVDGATLAR LDLEKKVESL LDELAFVRQV HDEEVAELLA TLQASSQAAA
260 270 280 290 300
EVDVAVAKPD LTSALREIRA QYESLAAKNL QSAEEWYKSK FANLNEQAAR
310 320 330 340 350
STEAIRASRE EIHEYRRQLQ ARTIEIEGLR GANESLERQI LELEERHSAE
360 370 380 390 400
VAGYQDSIGQ LESDLRNTKS EMARHLREYQ DLLNVKMALD IEIAAYRKLL
410 420 430 440 450
EGEETRFSTG GLSISGLNPL PNPSYLLPPR ILSSTASKVS SAGLSLKKEE
460 470 480 490 500
EEEEEEASKE VSKKTSKVGE GFEETLGEAV ISTKKTGKSA TEESTSSSQK

M
Length:501
Mass (Da):55,383
Last modified:January 22, 2014 - v3
Checksum:iB3DEF649DEB29C3B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28R → P in AAB37740 (PubMed:8734438).Curated1
Sequence conflicti34G → A in AAB37740 (PubMed:8734438).Curated1
Sequence conflicti141E → Q in AAB37740 (PubMed:8734438).Curated1
Sequence conflicti147L → R in AAB37740 (PubMed:8734438).Curated1
Sequence conflicti163Q → E in AAA62617 (PubMed:7959004).Curated1
Sequence conflicti297Q → L in AAB37740 (PubMed:8734438).Curated1
Sequence conflicti301S → T in AAB37740 (PubMed:8734438).Curated1
Sequence conflicti318 – 319QL → HV in AAB37740 (PubMed:8734438).Curated2
Sequence conflicti347H → D in AAA62617 (PubMed:7959004).Curated1
Sequence conflicti383L → F in AAA62617 (PubMed:7959004).Curated1
Sequence conflicti388A → G in AAA62617 (PubMed:7959004).Curated1
Sequence conflicti403E → N in AAB37740 (PubMed:8734438).Curated1
Sequence conflicti442A → T in AAA62617 (PubMed:7959004).Curated1
Sequence conflicti442A → T in AAB37740 (PubMed:8734438).Curated1
Sequence conflicti443G → R in AAA62617 (PubMed:7959004).Curated1
Sequence conflicti450E → D in AAA62617 (PubMed:7959004).Curated1
Sequence conflicti450E → D in AAB37740 (PubMed:8734438).Curated1
Sequence conflicti455E → EEEE in AAA62617 (PubMed:7959004).Curated1
Sequence conflicti456E → D in AAA62617 (PubMed:7959004).Curated1
Sequence conflicti456E → D in AAB37740 (PubMed:8734438).Curated1
Sequence conflicti489S → L in AAB37740 (PubMed:8734438).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27220 Genomic DNA. Translation: AAA62617.1.
L36390 mRNA. Translation: AAB37740.1.
BC018383 mRNA. Translation: AAH18383.1.
CCDSiCCDS38013.1.
PIRiI53868.
RefSeqiNP_666212.3. NM_146100.4.
UniGeneiMm.276251.

Genome annotation databases

EnsembliENSMUST00000037636; ENSMUSP00000041347; ENSMUSG00000034336.
GeneIDi226180.
KEGGimmu:226180.
UCSCiuc008huk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27220 Genomic DNA. Translation: AAA62617.1.
L36390 mRNA. Translation: AAB37740.1.
BC018383 mRNA. Translation: AAH18383.1.
CCDSiCCDS38013.1.
PIRiI53868.
RefSeqiNP_666212.3. NM_146100.4.
UniGeneiMm.276251.

3D structure databases

ProteinModelPortaliP46660.
SMRiP46660.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230487. 5 interactors.
IntActiP46660. 9 interactors.
MINTiMINT-136763.
STRINGi10090.ENSMUSP00000041347.

PTM databases

iPTMnetiP46660.
PhosphoSitePlusiP46660.
SwissPalmiP46660.

2D gel databases

UCD-2DPAGEP46660.

Proteomic databases

MaxQBiP46660.
PaxDbiP46660.
PeptideAtlasiP46660.
PRIDEiP46660.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037636; ENSMUSP00000041347; ENSMUSG00000034336.
GeneIDi226180.
KEGGimmu:226180.
UCSCiuc008huk.1. mouse.

Organism-specific databases

CTDi9118.
MGIiMGI:96568. Ina.

Phylogenomic databases

eggNOGiENOG410IGME. Eukaryota.
ENOG410XPTM. LUCA.
GeneTreeiENSGT00830000128228.
HOGENOMiHOG000230977.
HOVERGENiHBG013015.
InParanoidiP46660.
KOiK07608.
OMAiASSYRKV.
OrthoDBiEOG091G12MK.
TreeFamiTF330122.

Miscellaneous databases

ChiTaRSiIna. mouse.
PROiP46660.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034336.
GenevisibleiP46660. MM.

Family and domain databases

InterProiIPR027703. Alpha-Inx.
IPR001664. IF.
IPR006821. Intermed_filament_DNA-bd.
IPR018039. Intermediate_filament_CS.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PTHR23239:SF132. PTHR23239:SF132. 1 hit.
PfamiPF00038. Filament. 1 hit.
PF04732. Filament_head. 1 hit.
[Graphical view]
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAINX_MOUSE
AccessioniPrimary (citable) accession number: P46660
Secondary accession number(s): Q61958, Q8VCW5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 22, 2014
Last modified: November 2, 2016
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.