Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Adrenodoxin, mitochondrial

Gene

Fdx1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential for the synthesis of various steroid hormones, participates in the reduction of mitochondrial cytochrome P450 for steroidogenesis. Transfers electrons from adrenodoxin reductase to CYP11A1, a cytochrome P450 that catalyzes cholesterol side-chain cleavage (By similarity).By similarity

Cofactori

[2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi110 – 1101Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
Metal bindingi116 – 1161Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
Metal bindingi119 – 1191Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
Metal bindingi156 – 1561Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB
  2. electron carrier activity Source: UniProtKB
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cholesterol metabolic process Source: UniProtKB
  2. hormone biosynthetic process Source: UniProtKB
  3. oxidation-reduction process Source: UniProtKB-KW
  4. steroid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cholesterol metabolism, Electron transport, Lipid metabolism, Steroid metabolism, Steroidogenesis, Sterol metabolism, Transport

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_196619. Electron transport from NADPH to Ferredoxin.
REACT_199053. Mitochondrial iron-sulfur cluster biogenesis.
REACT_223561. Pregnenolone biosynthesis.
REACT_227038. Endogenous sterols.

Names & Taxonomyi

Protein namesi
Recommended name:
Adrenodoxin, mitochondrial
Alternative name(s):
Adrenal ferredoxin
Ferredoxin-1
Gene namesi
Name:Fdx1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:103224. Fdx1.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-SubCell
  2. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6464MitochondrionBy similarityAdd
BLAST
Chaini65 – 188124Adrenodoxin, mitochondrialPRO_0000000989Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei70 – 701N6-acetyllysine; alternate1 Publication
Modified residuei70 – 701N6-succinyllysine; alternate1 Publication
Modified residuei162 – 1621N6-succinyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP46656.
PaxDbiP46656.
PRIDEiP46656.

PTM databases

PhosphoSiteiP46656.

Expressioni

Gene expression databases

BgeeiP46656.
CleanExiMM_FDX1.
ExpressionAtlasiP46656. baseline and differential.
GenevestigatoriP46656.

Interactioni

Subunit structurei

Interacts with CYP11A1.By similarity

Protein-protein interaction databases

MINTiMINT-1861080.

Structurei

3D structure databases

ProteinModelPortaliP46656.
SMRiP46656. Positions 65-172.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini69 – 1751072Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the adrenodoxin/putidaredoxin family.Curated
Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0633.
HOGENOMiHOG000244518.
HOVERGENiHBG000092.
InParanoidiP46656.
OMAiDHIFEKL.
OrthoDBiEOG708W1D.
PhylomeDBiP46656.
TreeFamiTF319845.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR001055. Adrenodoxin.
IPR018298. Adrenodoxin_Fe-S_BS.
IPR012675. Beta-grasp_dom.
[Graphical view]
PfamiPF00111. Fer2. 1 hit.
[Graphical view]
PRINTSiPR00355. ADRENODOXIN.
SUPFAMiSSF54292. SSF54292. 1 hit.
PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
PS00814. ADX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46656-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAAPGARLL RAACASVPFR GLDRCRLLVC GTGAGTAISP WTPSPRLHAE
60 70 80 90 100
AGPGRPLSVS ARARSSSEDK ITVHFKNRDG ETLTTKGKIG DSLLDVVIEN
110 120 130 140 150
NLDIDGFGAC EGTLACSTCH LIFEDHIYEK LDAITDEEND MLDLAFGLTD
160 170 180
RSRLGCQVCL TKAMDNMTVR VPEAVADVRQ SVDMSKNS
Length:188
Mass (Da):20,123
Last modified:November 1, 1995 - v1
Checksum:i1118DE79BC8BDF48
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29123 mRNA. Translation: AAA74303.1.
D43689 mRNA. Translation: BAA07786.1.
D43690 mRNA. Translation: BAA07787.1.
CCDSiCCDS23178.1.
PIRiS53524.
RefSeqiNP_001288657.1. NM_001301728.1.
NP_032022.1. NM_007996.2.
UniGeneiMm.1061.
Mm.440190.

Genome annotation databases

EnsembliENSMUST00000034552; ENSMUSP00000034552; ENSMUSG00000032051.
GeneIDi14148.
KEGGimmu:14148.
UCSCiuc009plo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29123 mRNA. Translation: AAA74303.1.
D43689 mRNA. Translation: BAA07786.1.
D43690 mRNA. Translation: BAA07787.1.
CCDSiCCDS23178.1.
PIRiS53524.
RefSeqiNP_001288657.1. NM_001301728.1.
NP_032022.1. NM_007996.2.
UniGeneiMm.1061.
Mm.440190.

3D structure databases

ProteinModelPortaliP46656.
SMRiP46656. Positions 65-172.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1861080.

PTM databases

PhosphoSiteiP46656.

Proteomic databases

MaxQBiP46656.
PaxDbiP46656.
PRIDEiP46656.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034552; ENSMUSP00000034552; ENSMUSG00000032051.
GeneIDi14148.
KEGGimmu:14148.
UCSCiuc009plo.1. mouse.

Organism-specific databases

CTDi2230.
MGIiMGI:103224. Fdx1.

Phylogenomic databases

eggNOGiCOG0633.
HOGENOMiHOG000244518.
HOVERGENiHBG000092.
InParanoidiP46656.
OMAiDHIFEKL.
OrthoDBiEOG708W1D.
PhylomeDBiP46656.
TreeFamiTF319845.

Enzyme and pathway databases

ReactomeiREACT_196619. Electron transport from NADPH to Ferredoxin.
REACT_199053. Mitochondrial iron-sulfur cluster biogenesis.
REACT_223561. Pregnenolone biosynthesis.
REACT_227038. Endogenous sterols.

Miscellaneous databases

NextBioi285254.
PROiP46656.
SOURCEiSearch...

Gene expression databases

BgeeiP46656.
CleanExiMM_FDX1.
ExpressionAtlasiP46656. baseline and differential.
GenevestigatoriP46656.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR001055. Adrenodoxin.
IPR018298. Adrenodoxin_Fe-S_BS.
IPR012675. Beta-grasp_dom.
[Graphical view]
PfamiPF00111. Fer2. 1 hit.
[Graphical view]
PRINTSiPR00355. ADRENODOXIN.
SUPFAMiSSF54292. SSF54292. 1 hit.
PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
PS00814. ADX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Adrenal gland.
  2. "Mouse cytochrome P-450 linked ferredoxin: its cDNA cloning and inducibility by dibutyryladenosine 3',5'-cyclic monophosphate and forskolin."
    Itoh S., Iemura O., Yamada E., Yoshimura T., Tsujikawa K., Kohama Y., Mimura T.
    Biochim. Biophys. Acta 1263:173-175(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Kidney.
  3. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-70 AND LYS-162, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  4. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-70, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiADX_MOUSE
AccessioniPrimary (citable) accession number: P46656
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 7, 2015
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.