Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

40S ribosomal protein S0-B

Gene

RPS0B

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel (PubMed:22096102). uS2 is required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits (PubMed:9973221, PubMed:14627813).1 Publication2 Publications

Miscellaneous

Present with 60900 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for uS2 in yeast.Curated
This protein appears to have acquired a second function as a laminin receptor specifically in the vertebrate lineage. This protein does not bind laminin.1 Publication

GO - Molecular functioni

  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • ribosomal small subunit assembly Source: SGD
  • rRNA export from nucleus Source: SGD

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein
Biological processRibosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-32204-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S0-B1 PublicationUniRule annotation
Alternative name(s):
Nucleic acid-binding protein NAB1B
Small ribosomal subunit protein uS2-B1 Publication
Gene namesi
Name:RPS0B1 PublicationUniRule annotation
Synonyms:NAB1B, NAB4, YST2
Ordered Locus Names:YLR048W
ORF Names:L2118
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR048W.
SGDiS000004038. RPS0B.

Subcellular locationi

GO - Cellular componenti

  • 90S preribosome Source: GO_Central
  • cytosolic small ribosomal subunit Source: SGD

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Reduction in growth rate, a decrease in free 40S subunits, an increase in the amount of free 60S subunits and a decrease in polysome size.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedUniRule annotation2 Publications
ChainiPRO_00001343712 – 25240S ribosomal protein S0-BAdd BLAST251

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineUniRule annotation2 Publications1

Post-translational modificationi

N-terminally acetylated by acetyltransferase NatA.2 Publications

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP46654.
PRIDEiP46654.

PTM databases

iPTMnetiP46654.

Interactioni

Subunit structurei

Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102).1 Publication1 Publication

Protein-protein interaction databases

BioGridi31323. 310 interactors.
IntActiP46654. 91 interactors.
MINTiMINT-2787456.
STRINGi4932.YLR048W.

Structurei

3D structure databases

ProteinModelPortaliP46654.
SMRiP46654.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the universal ribosomal protein uS2 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000232073.
InParanoidiP46654.
KOiK02998.
OMAiRWICFST.
OrthoDBiEOG092C4HTX.

Family and domain databases

CDDicd01425. RPS2. 1 hit.
HAMAPiMF_03015. Ribosomal_S2_euk. 1 hit.
InterProiView protein in InterPro
IPR001865. Ribosomal_S2.
IPR018130. Ribosomal_S2_CS.
IPR027498. Ribosomal_S2_euk.
IPR005707. Ribosomal_S2_euk/arc.
IPR023591. Ribosomal_S2_flav_dom.
PANTHERiPTHR11489. PTHR11489. 1 hit.
PfamiView protein in Pfam
PF00318. Ribosomal_S2. 2 hits.
PRINTSiPR00395. RIBOSOMALS2.
SUPFAMiSSF52313. SSF52313. 1 hit.
TIGRFAMsiTIGR01012. uS2_euk_arch. 1 hit.
PROSITEiView protein in PROSITE
PS00962. RIBOSOMAL_S2_1. 1 hit.
PS00963. RIBOSOMAL_S2_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46654-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLPATFDLT PEDAQLLLAA NTHLGARNVQ VHQEPYVFNA RPDGVHVINV
60 70 80 90 100
GKTWEKLVLA ARIIAAIPNP EDVVAISSRT YGQRAVLKFA AHTGATPIAG
110 120 130 140 150
RFTPGSFTNY ITRSFKEPRL VIVTDPRLDA QAIKEASYVN IPVIALTDLD
160 170 180 190 200
SPSEFVDVAI PCNNRGKHSI GLIWYLLARE VLRLRGALVD RTQPWSIMPD
210 220 230 240 250
LYFYRNPEEV EQVAEEAAAA EEGEEEEVKE EVTEGQAEAT EWAEENADNV

EW
Length:252
Mass (Da):27,962
Last modified:January 23, 2007 - v2
Checksum:iB9B7CDFB335EFAC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33756 Genomic DNA. Translation: AAC49276.1.
X94607 Genomic DNA. Translation: CAA64295.1.
Z73220 Genomic DNA. Translation: CAA97578.1.
BK006945 Genomic DNA. Translation: DAA09366.1.
PIRiS59364.
RefSeqiNP_013149.1. NM_001181935.1.

Genome annotation databases

EnsemblFungiiYLR048W; YLR048W; YLR048W.
GeneIDi850737.
KEGGisce:YLR048W.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRSSA2_YEAST
AccessioniPrimary (citable) accession number: P46654
Secondary accession number(s): D6VY50
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: July 5, 2017
This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Ribosomal proteins
    Ribosomal proteins families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names