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P46654

- RSSA2_YEAST

UniProt

P46654 - RSSA2_YEAST

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Protein

40S ribosomal protein S0-B

Gene
RPS0B, NAB1B, NAB4, YST2, YLR048W, L2118
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits.2 Publications

GO - Molecular functioni

  1. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. cytoplasmic translation Source: SGD
  2. endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  3. endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  4. ribosomal small subunit assembly Source: SGD
  5. rRNA export from nucleus Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-32204-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S0-B
Alternative name(s):
Nucleic acid-binding protein NAB1B
Gene namesi
Name:RPS0B
Synonyms:NAB1B, NAB4, YST2
Ordered Locus Names:YLR048W
ORF Names:L2118
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

SGDiS000004038. RPS0B.

Subcellular locationi

Cytoplasm 2 Publications

GO - Cellular componenti

  1. cytosolic small ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Reduction in growth rate, a decrease in free 40S subunits, an increase in the amount of free 60S subunits and a decrease in polysome size.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 25225140S ribosomal protein S0-BUniRule annotationPRO_0000134371Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine2 Publications

Post-translational modificationi

N-terminally acetylated by acetyltransferase NatA.2 Publications

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP46654.
PaxDbiP46654.
PeptideAtlasiP46654.

Expressioni

Gene expression databases

GenevestigatoriP46654.

Interactioni

Subunit structurei

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).2 Publications

Protein-protein interaction databases

BioGridi31323. 102 interactions.
IntActiP46654. 89 interactions.
MINTiMINT-2787456.
STRINGi4932.YLR048W.

Structurei

3D structure databases

ProteinModelPortaliP46654.
SMRiP46654. Positions 2-207.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0052.
GeneTreeiENSGT00390000015036.
HOGENOMiHOG000232073.
KOiK02998.
OMAiESAYANV.
OrthoDBiEOG741ZD0.

Family and domain databases

HAMAPiMF_03015. Ribosomal_S2_euk.
InterProiIPR001865. Ribosomal_S2.
IPR018130. Ribosomal_S2_CS.
IPR027498. Ribosomal_S2_euk.
IPR005707. Ribosomal_S2_euk/arc.
IPR023591. Ribosomal_S2_flav_dom.
[Graphical view]
PANTHERiPTHR11489. PTHR11489. 1 hit.
PfamiPF00318. Ribosomal_S2. 2 hits.
[Graphical view]
PRINTSiPR00395. RIBOSOMALS2.
SUPFAMiSSF52313. SSF52313. 1 hit.
TIGRFAMsiTIGR01012. Sa_S2_E_A. 1 hit.
PROSITEiPS00962. RIBOSOMAL_S2_1. 1 hit.
PS00963. RIBOSOMAL_S2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46654-1 [UniParc]FASTAAdd to Basket

« Hide

MSLPATFDLT PEDAQLLLAA NTHLGARNVQ VHQEPYVFNA RPDGVHVINV    50
GKTWEKLVLA ARIIAAIPNP EDVVAISSRT YGQRAVLKFA AHTGATPIAG 100
RFTPGSFTNY ITRSFKEPRL VIVTDPRLDA QAIKEASYVN IPVIALTDLD 150
SPSEFVDVAI PCNNRGKHSI GLIWYLLARE VLRLRGALVD RTQPWSIMPD 200
LYFYRNPEEV EQVAEEAAAA EEGEEEEVKE EVTEGQAEAT EWAEENADNV 250
EW 252
Length:252
Mass (Da):27,962
Last modified:January 23, 2007 - v2
Checksum:iB9B7CDFB335EFAC3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U33756 Genomic DNA. Translation: AAC49276.1.
X94607 Genomic DNA. Translation: CAA64295.1.
Z73220 Genomic DNA. Translation: CAA97578.1.
BK006945 Genomic DNA. Translation: DAA09366.1.
PIRiS59364.
RefSeqiNP_013149.1. NM_001181935.1.

Genome annotation databases

EnsemblFungiiYLR048W; YLR048W; YLR048W.
GeneIDi850737.
KEGGisce:YLR048W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U33756 Genomic DNA. Translation: AAC49276.1 .
X94607 Genomic DNA. Translation: CAA64295.1 .
Z73220 Genomic DNA. Translation: CAA97578.1 .
BK006945 Genomic DNA. Translation: DAA09366.1 .
PIRi S59364.
RefSeqi NP_013149.1. NM_001181935.1.

3D structure databases

ProteinModelPortali P46654.
SMRi P46654. Positions 2-207.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31323. 102 interactions.
IntActi P46654. 89 interactions.
MINTi MINT-2787456.
STRINGi 4932.YLR048W.

Proteomic databases

MaxQBi P46654.
PaxDbi P46654.
PeptideAtlasi P46654.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLR048W ; YLR048W ; YLR048W .
GeneIDi 850737.
KEGGi sce:YLR048W.

Organism-specific databases

SGDi S000004038. RPS0B.

Phylogenomic databases

eggNOGi COG0052.
GeneTreei ENSGT00390000015036.
HOGENOMi HOG000232073.
KOi K02998.
OMAi ESAYANV.
OrthoDBi EOG741ZD0.

Enzyme and pathway databases

BioCyci YEAST:G3O-32204-MONOMER.
Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.

Miscellaneous databases

NextBioi 966843.

Gene expression databases

Genevestigatori P46654.

Family and domain databases

HAMAPi MF_03015. Ribosomal_S2_euk.
InterProi IPR001865. Ribosomal_S2.
IPR018130. Ribosomal_S2_CS.
IPR027498. Ribosomal_S2_euk.
IPR005707. Ribosomal_S2_euk/arc.
IPR023591. Ribosomal_S2_flav_dom.
[Graphical view ]
PANTHERi PTHR11489. PTHR11489. 1 hit.
Pfami PF00318. Ribosomal_S2. 2 hits.
[Graphical view ]
PRINTSi PR00395. RIBOSOMALS2.
SUPFAMi SSF52313. SSF52313. 1 hit.
TIGRFAMsi TIGR01012. Sa_S2_E_A. 1 hit.
PROSITEi PS00962. RIBOSOMAL_S2_1. 1 hit.
PS00963. RIBOSOMAL_S2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Yeast proteins related to the p40/laminin receptor precursor are essential components of the 40 S ribosomal subunit."
    Demianova M.A., Formosa T.G., Ellis S.R.
    J. Biol. Chem. 271:11383-11391(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Bienvenut W.V., Peters C.
    Submitted (MAY-2005) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-27; 63-79 AND 89-134, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY.
  5. "Metabolic and regulatory changes associated with growth of Saccharomyces cerevisiae in 1.4 M NaCl. Evidence for osmotic induction of glycerol dissimilation via the dihydroxyacetone pathway."
    Norbeck J., Blomberg A.
    J. Biol. Chem. 272:5544-5554(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 53-56; 102-111 AND 135-146.
    Strain: ATCC 44827 / SKQ2N.
  6. "The 67-kDa laminin receptor originated from a ribosomal protein that acquired a dual function during evolution."
    Ardini E., Pesole G., Tagliabue E., Magnifico A., Castronovo V., Sobel M.E., Colnaghi M.I., Menard S.
    Mol. Biol. Evol. 15:1017-1025(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE EVOLUTION.
  7. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  8. "Yeast proteins related to the p40/laminin receptor precursor are required for 20S ribosomal RNA processing and the maturation of 40S ribosomal subunits."
    Ford C.L., Randal-Whitis L., Ellis S.R.
    Cancer Res. 59:704-710(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  9. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
    Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
    J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
  10. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  12. "Ribosomal proteins Rps0 and Rps21 of Saccharomyces cerevisiae have overlapping functions in the maturation of the 3' end of 18S rRNA."
    Tabb-Massey A., Caffrey J.M., Logsden P., Taylor S., Trent J.O., Ellis S.R.
    Nucleic Acids Res. 31:6798-6805(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiRSSA2_YEAST
AccessioniPrimary (citable) accession number: P46654
Secondary accession number(s): D6VY50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 60900 molecules/cell in log phase SD medium.
There are 2 genes for S0 in yeast.
This protein appears to have acquired a second function as a laminin receptor specifically in the vertebrate lineage. This protein does not bind laminin.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Ribosomal proteins
    Ribosomal proteins families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3

Similar proteinsi