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P46654 (RSSA2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
40S ribosomal protein S0-B
Alternative name(s):
Nucleic acid-binding protein NAB1B
Gene names
Name:RPS0B
Synonyms:NAB1B, NAB4, YST2
Ordered Locus Names:YLR048W
ORF Names:L2118
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length252 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Ref.8 Ref.12

Subunit structure

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.7 Ref.8

Subcellular location

Cytoplasm Ref.10 Ref.12.

Post-translational modification

N-terminally acetylated by acetyltransferase NatA. Ref.4 Ref.9

Disruption phenotype

Reduction in growth rate, a decrease in free 40S subunits, an increase in the amount of free 60S subunits and a decrease in polysome size. Ref.12

Miscellaneous

Present with 60900 molecules/cell in log phase SD medium.

There are 2 genes for S0 in yeast.

This protein appears to have acquired a second function as a laminin receptor specifically in the vertebrate lineage. This protein does not bind laminin.

Sequence similarities

Belongs to the ribosomal protein S2P family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.4 Ref.9
Chain2 – 25225140S ribosomal protein S0-B HAMAP-Rule MF_03015
PRO_0000134371

Amino acid modifications

Modified residue21N-acetylserine Ref.4 Ref.9

Sequences

Sequence LengthMass (Da)Tools
P46654 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: B9B7CDFB335EFAC3

FASTA25227,962
        10         20         30         40         50         60 
MSLPATFDLT PEDAQLLLAA NTHLGARNVQ VHQEPYVFNA RPDGVHVINV GKTWEKLVLA 

        70         80         90        100        110        120 
ARIIAAIPNP EDVVAISSRT YGQRAVLKFA AHTGATPIAG RFTPGSFTNY ITRSFKEPRL 

       130        140        150        160        170        180 
VIVTDPRLDA QAIKEASYVN IPVIALTDLD SPSEFVDVAI PCNNRGKHSI GLIWYLLARE 

       190        200        210        220        230        240 
VLRLRGALVD RTQPWSIMPD LYFYRNPEEV EQVAEEAAAA EEGEEEEVKE EVTEGQAEAT 

       250 
EWAEENADNV EW 

« Hide

References

« Hide 'large scale' references
[1]"Yeast proteins related to the p40/laminin receptor precursor are essential components of the 40 S ribosomal subunit."
Demianova M.A., Formosa T.G., Ellis S.R.
J. Biol. Chem. 271:11383-11391(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]Bienvenut W.V., Peters C.
Submitted (MAY-2005) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 2-27; 63-79 AND 89-134, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY.
[5]"Metabolic and regulatory changes associated with growth of Saccharomyces cerevisiae in 1.4 M NaCl. Evidence for osmotic induction of glycerol dissimilation via the dihydroxyacetone pathway."
Norbeck J., Blomberg A.
J. Biol. Chem. 272:5544-5554(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 53-56; 102-111 AND 135-146.
Strain: ATCC 44827 / SKQ2N.
[6]"The 67-kDa laminin receptor originated from a ribosomal protein that acquired a dual function during evolution."
Ardini E., Pesole G., Tagliabue E., Magnifico A., Castronovo V., Sobel M.E., Colnaghi M.I., Menard S.
Mol. Biol. Evol. 15:1017-1025(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE EVOLUTION.
[7]"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
Planta R.J., Mager W.H.
Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE, SUBUNIT.
[8]"Yeast proteins related to the p40/laminin receptor precursor are required for 20S ribosomal RNA processing and the maturation of 40S ribosomal subunits."
Ford C.L., Randal-Whitis L., Ellis S.R.
Cancer Res. 59:704-710(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT.
[9]"The action of N-terminal acetyltransferases on yeast ribosomal proteins."
Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
[10]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[11]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[12]"Ribosomal proteins Rps0 and Rps21 of Saccharomyces cerevisiae have overlapping functions in the maturation of the 3' end of 18S rRNA."
Tabb-Massey A., Caffrey J.M., Logsden P., Taylor S., Trent J.O., Ellis S.R.
Nucleic Acids Res. 31:6798-6805(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U33756 Genomic DNA. Translation: AAC49276.1.
X94607 Genomic DNA. Translation: CAA64295.1.
Z73220 Genomic DNA. Translation: CAA97578.1.
BK006945 Genomic DNA. Translation: DAA09366.1.
PIRS59364.
RefSeqNP_013149.1. NM_001181935.1.

3D structure databases

ProteinModelPortalP46654.
SMRP46654. Positions 2-207.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31323. 102 interactions.
IntActP46654. 89 interactions.
MINTMINT-2787456.
STRING4932.YLR048W.

Proteomic databases

MaxQBP46654.
PaxDbP46654.
PeptideAtlasP46654.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR048W; YLR048W; YLR048W.
GeneID850737.
KEGGsce:YLR048W.

Organism-specific databases

SGDS000004038. RPS0B.

Phylogenomic databases

eggNOGCOG0052.
GeneTreeENSGT00390000015036.
HOGENOMHOG000232073.
KOK02998.
OMAESAYANV.
OrthoDBEOG741ZD0.

Enzyme and pathway databases

BioCycYEAST:G3O-32204-MONOMER.

Gene expression databases

GenevestigatorP46654.

Family and domain databases

HAMAPMF_03015. Ribosomal_S2_euk.
InterProIPR001865. Ribosomal_S2.
IPR018130. Ribosomal_S2_CS.
IPR027498. Ribosomal_S2_euk.
IPR005707. Ribosomal_S2_euk/arc.
IPR023591. Ribosomal_S2_flav_dom.
[Graphical view]
PANTHERPTHR11489. PTHR11489. 1 hit.
PfamPF00318. Ribosomal_S2. 2 hits.
[Graphical view]
PRINTSPR00395. RIBOSOMALS2.
SUPFAMSSF52313. SSF52313. 1 hit.
TIGRFAMsTIGR01012. Sa_S2_E_A. 1 hit.
PROSITEPS00962. RIBOSOMAL_S2_1. 1 hit.
PS00963. RIBOSOMAL_S2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio966843.

Entry information

Entry nameRSSA2_YEAST
AccessionPrimary (citable) accession number: P46654
Secondary accession number(s): D6VY50
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries