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P46654

- RSSA2_YEAST

UniProt

P46654 - RSSA2_YEAST

Protein

40S ribosomal protein S0-B

Gene

RPS0B

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits.2 PublicationsUniRule annotation

    GO - Molecular functioni

    1. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD
    2. endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
    3. endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
    4. ribosomal small subunit assembly Source: SGD
    5. rRNA export from nucleus Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Keywords - Biological processi

    Ribosome biogenesis, rRNA processing

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32204-MONOMER.
    ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    40S ribosomal protein S0-BUniRule annotation
    Alternative name(s):
    Nucleic acid-binding protein NAB1B
    Gene namesi
    Name:RPS0BUniRule annotation
    Synonyms:NAB1B, NAB4, YST2
    Ordered Locus Names:YLR048W
    ORF Names:L2118
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    SGDiS000004038. RPS0B.

    Subcellular locationi

    Cytoplasm 2 PublicationsUniRule annotation

    GO - Cellular componenti

    1. cytosolic small ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Reduction in growth rate, a decrease in free 40S subunits, an increase in the amount of free 60S subunits and a decrease in polysome size.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 PublicationsUniRule annotation
    Chaini2 – 25225140S ribosomal protein S0-BPRO_0000134371Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine2 PublicationsUniRule annotation

    Post-translational modificationi

    N-terminally acetylated by acetyltransferase NatA.2 Publications

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP46654.
    PaxDbiP46654.
    PeptideAtlasiP46654.

    Expressioni

    Gene expression databases

    GenevestigatoriP46654.

    Interactioni

    Subunit structurei

    Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).2 Publications

    Protein-protein interaction databases

    BioGridi31323. 102 interactions.
    IntActiP46654. 89 interactions.
    MINTiMINT-2787456.
    STRINGi4932.YLR048W.

    Structurei

    3D structure databases

    ProteinModelPortaliP46654.
    SMRiP46654. Positions 2-207.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein S2P family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0052.
    GeneTreeiENSGT00390000015036.
    HOGENOMiHOG000232073.
    KOiK02998.
    OMAiESAYANV.
    OrthoDBiEOG741ZD0.

    Family and domain databases

    HAMAPiMF_03015. Ribosomal_S2_euk.
    InterProiIPR001865. Ribosomal_S2.
    IPR018130. Ribosomal_S2_CS.
    IPR027498. Ribosomal_S2_euk.
    IPR005707. Ribosomal_S2_euk/arc.
    IPR023591. Ribosomal_S2_flav_dom.
    [Graphical view]
    PANTHERiPTHR11489. PTHR11489. 1 hit.
    PfamiPF00318. Ribosomal_S2. 2 hits.
    [Graphical view]
    PRINTSiPR00395. RIBOSOMALS2.
    SUPFAMiSSF52313. SSF52313. 1 hit.
    TIGRFAMsiTIGR01012. Sa_S2_E_A. 1 hit.
    PROSITEiPS00962. RIBOSOMAL_S2_1. 1 hit.
    PS00963. RIBOSOMAL_S2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P46654-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLPATFDLT PEDAQLLLAA NTHLGARNVQ VHQEPYVFNA RPDGVHVINV    50
    GKTWEKLVLA ARIIAAIPNP EDVVAISSRT YGQRAVLKFA AHTGATPIAG 100
    RFTPGSFTNY ITRSFKEPRL VIVTDPRLDA QAIKEASYVN IPVIALTDLD 150
    SPSEFVDVAI PCNNRGKHSI GLIWYLLARE VLRLRGALVD RTQPWSIMPD 200
    LYFYRNPEEV EQVAEEAAAA EEGEEEEVKE EVTEGQAEAT EWAEENADNV 250
    EW 252
    Length:252
    Mass (Da):27,962
    Last modified:January 23, 2007 - v2
    Checksum:iB9B7CDFB335EFAC3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U33756 Genomic DNA. Translation: AAC49276.1.
    X94607 Genomic DNA. Translation: CAA64295.1.
    Z73220 Genomic DNA. Translation: CAA97578.1.
    BK006945 Genomic DNA. Translation: DAA09366.1.
    PIRiS59364.
    RefSeqiNP_013149.1. NM_001181935.1.

    Genome annotation databases

    EnsemblFungiiYLR048W; YLR048W; YLR048W.
    GeneIDi850737.
    KEGGisce:YLR048W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U33756 Genomic DNA. Translation: AAC49276.1 .
    X94607 Genomic DNA. Translation: CAA64295.1 .
    Z73220 Genomic DNA. Translation: CAA97578.1 .
    BK006945 Genomic DNA. Translation: DAA09366.1 .
    PIRi S59364.
    RefSeqi NP_013149.1. NM_001181935.1.

    3D structure databases

    ProteinModelPortali P46654.
    SMRi P46654. Positions 2-207.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31323. 102 interactions.
    IntActi P46654. 89 interactions.
    MINTi MINT-2787456.
    STRINGi 4932.YLR048W.

    Proteomic databases

    MaxQBi P46654.
    PaxDbi P46654.
    PeptideAtlasi P46654.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR048W ; YLR048W ; YLR048W .
    GeneIDi 850737.
    KEGGi sce:YLR048W.

    Organism-specific databases

    SGDi S000004038. RPS0B.

    Phylogenomic databases

    eggNOGi COG0052.
    GeneTreei ENSGT00390000015036.
    HOGENOMi HOG000232073.
    KOi K02998.
    OMAi ESAYANV.
    OrthoDBi EOG741ZD0.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32204-MONOMER.
    Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Miscellaneous databases

    NextBioi 966843.

    Gene expression databases

    Genevestigatori P46654.

    Family and domain databases

    HAMAPi MF_03015. Ribosomal_S2_euk.
    InterProi IPR001865. Ribosomal_S2.
    IPR018130. Ribosomal_S2_CS.
    IPR027498. Ribosomal_S2_euk.
    IPR005707. Ribosomal_S2_euk/arc.
    IPR023591. Ribosomal_S2_flav_dom.
    [Graphical view ]
    PANTHERi PTHR11489. PTHR11489. 1 hit.
    Pfami PF00318. Ribosomal_S2. 2 hits.
    [Graphical view ]
    PRINTSi PR00395. RIBOSOMALS2.
    SUPFAMi SSF52313. SSF52313. 1 hit.
    TIGRFAMsi TIGR01012. Sa_S2_E_A. 1 hit.
    PROSITEi PS00962. RIBOSOMAL_S2_1. 1 hit.
    PS00963. RIBOSOMAL_S2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Yeast proteins related to the p40/laminin receptor precursor are essential components of the 40 S ribosomal subunit."
      Demianova M.A., Formosa T.G., Ellis S.R.
      J. Biol. Chem. 271:11383-11391(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Bienvenut W.V., Peters C.
      Submitted (MAY-2005) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 2-27; 63-79 AND 89-134, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY.
    5. "Metabolic and regulatory changes associated with growth of Saccharomyces cerevisiae in 1.4 M NaCl. Evidence for osmotic induction of glycerol dissimilation via the dihydroxyacetone pathway."
      Norbeck J., Blomberg A.
      J. Biol. Chem. 272:5544-5554(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 53-56; 102-111 AND 135-146.
      Strain: ATCC 44827 / SKQ2N.
    6. "The 67-kDa laminin receptor originated from a ribosomal protein that acquired a dual function during evolution."
      Ardini E., Pesole G., Tagliabue E., Magnifico A., Castronovo V., Sobel M.E., Colnaghi M.I., Menard S.
      Mol. Biol. Evol. 15:1017-1025(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE EVOLUTION.
    7. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
      Planta R.J., Mager W.H.
      Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.
    8. "Yeast proteins related to the p40/laminin receptor precursor are required for 20S ribosomal RNA processing and the maturation of 40S ribosomal subunits."
      Ford C.L., Randal-Whitis L., Ellis S.R.
      Cancer Res. 59:704-710(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT.
    9. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
      Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
      J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
    10. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    12. "Ribosomal proteins Rps0 and Rps21 of Saccharomyces cerevisiae have overlapping functions in the maturation of the 3' end of 18S rRNA."
      Tabb-Massey A., Caffrey J.M., Logsden P., Taylor S., Trent J.O., Ellis S.R.
      Nucleic Acids Res. 31:6798-6805(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiRSSA2_YEAST
    AccessioniPrimary (citable) accession number: P46654
    Secondary accession number(s): D6VY50
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 124 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 60900 molecules/cell in log phase SD medium.1 Publication
    There are 2 genes for S0 in yeast.
    This protein appears to have acquired a second function as a laminin receptor specifically in the vertebrate lineage. This protein does not bind laminin.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Ribosomal proteins
      Ribosomal proteins families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3