40S ribosomal protein S0-B - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P46654 (RSSA2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 114. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
40S ribosomal protein S0-B

Alternative name(s):
Nucleic acid-binding protein NAB1B

Gene names

Name:	RPS0B
Synonyms:	NAB1B, NAB4, YST2
Ordered Locus Names:	YLR048W
ORF Names:	L2118

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	252 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Ref.8 Ref.12
Subunit structure	Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.7 Ref.8
Subcellular location	Cytoplasm Ref.10 Ref.12.
Post-translational modification	N-terminally acetylated by acetyltransferase NatA. HAMAP-Rule MF_03015
Disruption phenotype	Reduction in growth rate, a decrease in free 40S subunits, an increase in the amount of free 60S subunits and a decrease in polysome size. Ref.12
Miscellaneous	Present with 60900 molecules/cell in log phase SD medium. HAMAP-Rule MF_03015 There are 2 genes for S0 in yeast. HAMAP-Rule MF_03015 This protein appears to have acquired a second function as a laminin receptor specifically in the vertebrate lineage. This protein does not bind laminin. HAMAP-Rule MF_03015
Sequence similarities	Belongs to the ribosomal protein S2P family.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Inferred from mutant phenotype Ref.1. Source: SGD endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype Ref.12. Source: SGD endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype Ref.12. Source: SGD rRNA export from nucleus Inferred from genetic interaction PubMed 16246728. Source: SGD ribosomal small subunit assembly Inferred from mutant phenotype Ref.1. Source: SGD
Cellular_component	90S preribosome Inferred from Biological aspect of Ancestor. Source: RefGenome cytosolic small ribosomal subunit Inferred from direct assay Ref.1. Source: SGD
Molecular_function	structural constituent of ribosome Inferred from direct assay Ref.1. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.4 Ref.9

Chain

2 – 252

251

40S ribosomal protein S0-B HAMAP-Rule MF_03015

PRO_0000134371

Amino acid modifications

Modified residue

N-acetylserine Ref.4 Ref.9

Modified residue

103

Phosphothreonine By similarity

Modified residue

112

Phosphothreonine Ref.13

Sequences

Sequence

Length

Mass (Da)

Tools

P46654 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: B9B7CDFB335EFAC3
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FASTA

252

27,962

        10         20         30         40         50         60 
MSLPATFDLT PEDAQLLLAA NTHLGARNVQ VHQEPYVFNA RPDGVHVINV GKTWEKLVLA 

        70         80         90        100        110        120 
ARIIAAIPNP EDVVAISSRT YGQRAVLKFA AHTGATPIAG RFTPGSFTNY ITRSFKEPRL 

       130        140        150        160        170        180 
VIVTDPRLDA QAIKEASYVN IPVIALTDLD SPSEFVDVAI PCNNRGKHSI GLIWYLLARE 

       190        200        210        220        230        240 
VLRLRGALVD RTQPWSIMPD LYFYRNPEEV EQVAEEAAAA EEGEEEEVKE EVTEGQAEAT 

       250 
EWAEENADNV EW

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast proteins related to the p40/laminin receptor precursor are essential components of the 40 S ribosomal subunit." Demianova M.A., Formosa T.G., Ellis S.R. J. Biol. Chem. 271:11383-11391(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. Hoheisel J.D. Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	Bienvenut W.V., Peters C. Submitted (MAY-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-27; 63-79 AND 89-134, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, MASS SPECTROMETRY.
[5]	"Metabolic and regulatory changes associated with growth of Saccharomyces cerevisiae in 1.4 M NaCl. Evidence for osmotic induction of glycerol dissimilation via the dihydroxyacetone pathway." Norbeck J., Blomberg A. J. Biol. Chem. 272:5544-5554(1997) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 53-56; 102-111 AND 135-146. Strain: ATCC 44827 / SKQ2N.
[6]	"The 67-kDa laminin receptor originated from a ribosomal protein that acquired a dual function during evolution." Ardini E., Pesole G., Tagliabue E., Magnifico A., Castronovo V., Sobel M.E., Colnaghi M.I., Menard S. Mol. Biol. Evol. 15:1017-1025(1998) [PubMed] [Europe PMC] [Abstract] Cited for: GENE EVOLUTION.
[7]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[8]	"Yeast proteins related to the p40/laminin receptor precursor are required for 20S ribosomal RNA processing and the maturation of 40S ribosomal subunits." Ford C.L., Randal-Whitis L., Ellis S.R. Cancer Res. 59:704-710(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT.
[9]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
[10]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[11]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[12]	"Ribosomal proteins Rps0 and Rps21 of Saccharomyces cerevisiae have overlapping functions in the maturation of the 3' end of 18S rRNA." Tabb-Massey A., Caffrey J.M., Logsden P., Taylor S., Trent J.O., Ellis S.R. Nucleic Acids Res. 31:6798-6805(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[13]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-112, MASS SPECTROMETRY. Strain: YAL6B.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U33756 Genomic DNA. Translation: AAC49276.1. X94607 Genomic DNA. Translation: CAA64295.1. Z73220 Genomic DNA. Translation: CAA97578.1. BK006945 Genomic DNA. Translation: DAA09366.1.
PIR	S59364.
RefSeq	NP_013149.1. NM_001181935.1.
3D structure databases
ProteinModelPortal	P46654.
SMR	P46654. Positions 2-207.
ModBase	Search...
Protein-protein interaction databases
IntAct	P46654. 86 interactions.
MINT	MINT-2787456.
STRING	4932.YLR048W.
Proteomic databases
PaxDb	P46654.
PeptideAtlas	P46654.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR048W; YLR048W; YLR048W.
GeneID	850737.
KEGG	sce:YLR048W.
Organism-specific databases
SGD	S000004038. RPS0B.
Phylogenomic databases
eggNOG	COG0052.
GeneTree	ENSGT00390000015036.
HOGENOM	HOG000232073.
KO	K02998.
OMA	FKEPRVI.
OrthoDB	EOG4KH646.
Enzyme and pathway databases
BioCyc	YEAST:G3O-32204-MONOMER.
Gene expression databases
Genevestigator	P46654.
GermOnline	YLR048W. Saccharomyces cerevisiae.
Family and domain databases
HAMAP	MF_03015. Ribosomal_S2_euk.
InterPro	IPR001865. Ribosomal_S2. IPR018130. Ribosomal_S2_CS. IPR027498. Ribosomal_S2_euk. IPR005707. Ribosomal_S2_euk/arc. IPR023591. Ribosomal_S2_flav_dom. [Graphical view]
PANTHER	PTHR11489. PTHR11489. 1 hit.
Pfam	PF00318. Ribosomal_S2. 2 hits. [Graphical view]
PRINTS	PR00395. RIBOSOMALS2.
SUPFAM	SSF52313. Ribosomal_S2. 1 hit.
TIGRFAMs	TIGR01012. Sa_S2_E_A. 1 hit.
PROSITE	PS00962. RIBOSOMAL_S2_1. 1 hit. PS00963. RIBOSOMAL_S2_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	966843.

Entry information

Entry name

RSSA2_YEAST

Accession

Primary (citable) accession number: P46654
Secondary accession number(s): D6VY50

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 114 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Ribosomal proteins

Ribosomal proteins families and list of entries

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents