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Protein

Aspartate aminotransferase 3, chloroplastic

Gene

ASP3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Amino acid aminotransferase important for the metabolism of amino acids and Krebs-cycle related organic acids. No activity with D-Asp or D-Ala as amino donors. In plants, it is involved in nitrogen metabolism and in aspects of carbon and energy metabolism.2 Publications

Catalytic activityi

L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.2 Publications

Cofactori

Kineticsi

  1. KM=2.5 mM for L-aspartate1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei81 – 811Aspartate; via amide nitrogenBy similarity
    Binding sitei178 – 1781AspartateBy similarity
    Binding sitei231 – 2311AspartateBy similarity
    Binding sitei423 – 4231AspartateBy similarity

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    ReactomeiR-ATH-70263. Gluconeogenesis.
    R-ATH-70614. Amino acid synthesis and interconversion (transamination).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aspartate aminotransferase 3, chloroplastic (EC:2.6.1.1)
    Alternative name(s):
    Protein YELLOW-LEAF-SPECIFIC GENE 4
    Transaminase A
    Gene namesi
    Name:ASP3
    Synonyms:YLS4
    Ordered Locus Names:At5g11520
    ORF Names:F15N18.110
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 5

    Organism-specific databases

    TAIRiAT5G11520.

    Subcellular locationi

    • Plastidchloroplast 1 Publication

    • Note: accumulates in stromules, which are membrane-bound protrusions of the plastid envelope containing soluble stroma. Stromules are often found connecting plastids within a cell.

    GO - Cellular componenti

    • chloroplast Source: UniProtKB-SubCell
    • membrane Source: TAIR
    • peroxisome Source: TAIR
    • plastid Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4343ChloroplastSequence analysisAdd
    BLAST
    Chaini44 – 449406Aspartate aminotransferase 3, chloroplasticPRO_0000001210Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei295 – 2951N6-(pyridoxal phosphate)lysineBy similarity

    Proteomic databases

    PaxDbiP46644.
    PRIDEiP46644.

    Expressioni

    Tissue specificityi

    Expressed in roots, cauline leaves, flowers, hypocotyl epidermis and root hair cells.2 Publications

    Developmental stagei

    Up-regulated in leaves during natural senescence.1 Publication

    Inductioni

    By ethylene and dark.1 Publication

    Gene expression databases

    GenevisibleiP46644. AT.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    GO - Molecular functioni

    Protein-protein interaction databases

    BioGridi16302. 2 interactions.
    IntActiP46644. 1 interaction.
    STRINGi3702.AT5G11520.1.

    Structurei

    3D structure databases

    ProteinModelPortaliP46644.
    SMRiP46644. Positions 47-444.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiKOG1411. Eukaryota.
    COG1448. LUCA.
    HOGENOMiHOG000185898.
    InParanoidiP46644.
    KOiK14454.
    OMAiGIYSMPP.
    OrthoDBiEOG09360C83.
    PhylomeDBiP46644.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    InterProiIPR004839. Aminotransferase_I/II.
    IPR000796. Asp_trans.
    IPR004838. NHTrfase_class1_PyrdxlP-BS.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    [Graphical view]
    PANTHERiPTHR11879. PTHR11879. 1 hit.
    PfamiPF00155. Aminotran_1_2. 1 hit.
    [Graphical view]
    PRINTSiPR00799. TRANSAMINASE.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiPS00105. AA_TRANSFER_CLASS_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P46644-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKTTHFSSSS SSDRRIGALL RHLNSGSDSD NLSSLYASPT SGGTGGSVFS
    60 70 80 90 100
    HLVQAPEDPI LGVTVAYNKD PSPVKLNLGV GAYRTEEGKP LVLNVVRKAE
    110 120 130 140 150
    QQLINDRTRI KEYLPIVGLV EFNKLSAKLI LGADSPAIRE NRITTVECLS
    160 170 180 190 200
    GTGSLRVGGE FLAKHYHQKT IYITQPTWGN HPKIFTLAGL TVKTYRYYDP
    210 220 230 240 250
    ATRGLNFQGL LEDLGAAAPG SIVLLHACAH NPTGVDPTIQ QWEQIRKLMR
    260 270 280 290 300
    SKGLMPFFDS AYQGFASGSL DTDAKPIRMF VADGGECLVA QSYAKNMGLY
    310 320 330 340 350
    GERVGALSIV CKSADVAGRV ESQLKLVIRP MYSSPPIHGA SIVAVILRDK
    360 370 380 390 400
    NLFNEWTLEL KAMADRIISM RKQLFEALRT RGTPGDWSHI IKQIGMFTFT
    410 420 430 440
    GLNPAQVSFM TKEYHIYMTS DGRISMAGLS SKTVPHLADA IHAVVTKAV
    Length:449
    Mass (Da):48,954
    Last modified:November 1, 1995 - v1
    Checksum:i8668EA40367C6A58
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U15034 mRNA. Translation: AAA79371.1.
    AL163815 Genomic DNA. Translation: CAB87712.1.
    CP002688 Genomic DNA. Translation: AED91691.1.
    AY050765 mRNA. Translation: AAK92700.1.
    AY079310 mRNA. Translation: AAL85041.1.
    AB047807 mRNA. Translation: BAB32884.1.
    PIRiT48511.
    RefSeqiNP_196713.1. NM_121190.2.
    UniGeneiAt.21687.

    Genome annotation databases

    EnsemblPlantsiAT5G11520.1; AT5G11520.1; AT5G11520.
    GeneIDi831024.
    GrameneiAT5G11520.1; AT5G11520.1; AT5G11520.
    KEGGiath:AT5G11520.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U15034 mRNA. Translation: AAA79371.1.
    AL163815 Genomic DNA. Translation: CAB87712.1.
    CP002688 Genomic DNA. Translation: AED91691.1.
    AY050765 mRNA. Translation: AAK92700.1.
    AY079310 mRNA. Translation: AAL85041.1.
    AB047807 mRNA. Translation: BAB32884.1.
    PIRiT48511.
    RefSeqiNP_196713.1. NM_121190.2.
    UniGeneiAt.21687.

    3D structure databases

    ProteinModelPortaliP46644.
    SMRiP46644. Positions 47-444.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi16302. 2 interactions.
    IntActiP46644. 1 interaction.
    STRINGi3702.AT5G11520.1.

    Proteomic databases

    PaxDbiP46644.
    PRIDEiP46644.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT5G11520.1; AT5G11520.1; AT5G11520.
    GeneIDi831024.
    GrameneiAT5G11520.1; AT5G11520.1; AT5G11520.
    KEGGiath:AT5G11520.

    Organism-specific databases

    TAIRiAT5G11520.

    Phylogenomic databases

    eggNOGiKOG1411. Eukaryota.
    COG1448. LUCA.
    HOGENOMiHOG000185898.
    InParanoidiP46644.
    KOiK14454.
    OMAiGIYSMPP.
    OrthoDBiEOG09360C83.
    PhylomeDBiP46644.

    Enzyme and pathway databases

    ReactomeiR-ATH-70263. Gluconeogenesis.
    R-ATH-70614. Amino acid synthesis and interconversion (transamination).

    Miscellaneous databases

    PROiP46644.

    Gene expression databases

    GenevisibleiP46644. AT.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    InterProiIPR004839. Aminotransferase_I/II.
    IPR000796. Asp_trans.
    IPR004838. NHTrfase_class1_PyrdxlP-BS.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    [Graphical view]
    PANTHERiPTHR11879. PTHR11879. 1 hit.
    PfamiPF00155. Aminotran_1_2. 1 hit.
    [Graphical view]
    PRINTSiPR00799. TRANSAMINASE.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiPS00105. AA_TRANSFER_CLASS_1. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiAAT3_ARATH
    AccessioniPrimary (citable) accession number: P46644
    Secondary accession number(s): Q9CAZ5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: September 7, 2016
    This is version 133 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.