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Reviewed, UniProtKB/Swiss-Prot P46644 (AAT3_ARATH)

Last modified November 3, 2009. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Aspartate aminotransferase, chloroplastic
    EC=2.6.1.1
Alternative name(s):
    Transaminase A
Gene names
Name: ASP3
Ordered Locus Names: At5g11520
ORF Names: F15N18_110
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length449 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Important for the metabolism of amino acids and Krebs-cycle related organic acids. In plants, it is involved in nitrogen metabolism and in aspects of carbon and energy metabolism.

Catalytic activity

L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.

Cofactor

Pyridoxal phosphate.

Subunit structure

Homodimer By similarity.

Subcellular location

Plastid › chloroplast Potential.

Miscellaneous

In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.

Sequence similarities

Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4343Chloroplast Potential
Chain44 – 449406Aspartate aminotransferase, chloroplastic
PRO_0000001210

Amino acid modifications

Modified residue2951N6-(pyridoxal phosphate)lysine By similarity

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Sequences

Sequence LengthMass (Da)Tools
P46644-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 8668EA40367C6A58

FASTA44948,954
        10         20         30         40         50         60 
MKTTHFSSSS SSDRRIGALL RHLNSGSDSD NLSSLYASPT SGGTGGSVFS HLVQAPEDPI 

        70         80         90        100        110        120 
LGVTVAYNKD PSPVKLNLGV GAYRTEEGKP LVLNVVRKAE QQLINDRTRI KEYLPIVGLV 

       130        140        150        160        170        180 
EFNKLSAKLI LGADSPAIRE NRITTVECLS GTGSLRVGGE FLAKHYHQKT IYITQPTWGN 

       190        200        210        220        230        240 
HPKIFTLAGL TVKTYRYYDP ATRGLNFQGL LEDLGAAAPG SIVLLHACAH NPTGVDPTIQ 

       250        260        270        280        290        300 
QWEQIRKLMR SKGLMPFFDS AYQGFASGSL DTDAKPIRMF VADGGECLVA QSYAKNMGLY 

       310        320        330        340        350        360 
GERVGALSIV CKSADVAGRV ESQLKLVIRP MYSSPPIHGA SIVAVILRDK NLFNEWTLEL 

       370        380        390        400        410        420 
KAMADRIISM RKQLFEALRT RGTPGDWSHI IKQIGMFTFT GLNPAQVSFM TKEYHIYMTS 

       430        440 
DGRISMAGLS SKTVPHLADA IHAVVTKAV

« Hide

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References

« Hide 'large scale' references
[1]"The aspartate aminotransferase gene family of Arabidopsis encodes isoenzymes localized to three distinct subcellular compartments."
Schultz C.J., Coruzzi G.M.
Plant J. 7:61-75(1995) [PubMed: 7894512] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
Tissue: Leaf.
[2]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed: 11130714] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
+Additional computationally mapped references.

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Cross-references

Sequence databases

U15034 mRNA. Translation: AAA79371.1.
AL163815 Genomic DNA. Translation: CAB87712.1.
AY050765 mRNA. Translation: AAK92700.1.
AY079310 mRNA. Translation: AAL85041.1.
IPIIPI00532023.
PIRT48511.
RefSeqNP_196713.1.
UniGeneAt.21687

3D structure databases

HSSPHSSP built from PDB template 7AAT based on UniProtKB P00508.
ModBaseSearch...

Protein-protein interaction databases

STRINGP46644.

Proteomic databases

PRIDEP46644.
ProMEXP46644.

Genome annotation databases

GeneID831024.
GenomeReviewsGene locus AT5G11520 in contig BA000015_GR.
KEGGath:AT5G11520.
NMPDRfig|3702.1.peg.23290.

Organism-specific databases

TAIRAt5g11520.

Phylogenomic databases

OMAIFGADSP.

Enzyme and pathway databases

BRENDA2.6.1.1. 302.

Gene expression databases

ArrayExpressP46644.
GenevestigatorP46644.
GermOnlineAT5G11520. Arabidopsis thaliana.

Family and domain databases

InterProIPR004839. Aminotransferase_I/II.
IPR000796. Asp_trans.
IPR004838. NHTrfase_class1_PyrdxlP-BS.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
Gene3DG3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHERPTHR11879. Asp_trans. 1 hit.
PfamPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
PRINTSPR00799. TRANSAMINASE.
PROSITEPS00105. AA_TRANSFER_CLASS_1. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameAAT3_ARATH
AccessionPrimary (citable) accession number: P46644
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 3, 2009
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents