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Protein

Aspartate aminotransferase 3, chloroplastic

Gene

ASP3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Amino acid aminotransferase important for the metabolism of amino acids and Krebs-cycle related organic acids. No activity with D-Asp or D-Ala as amino donors. In plants, it is involved in nitrogen metabolism and in aspects of carbon and energy metabolism.2 Publications

Miscellaneous

In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.

Catalytic activityi

L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.2 Publications

Cofactori

Kineticsi

  1. KM=2.5 mM for L-aspartate1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei81Aspartate; via amide nitrogenBy similarity1
    Binding sitei178AspartateBy similarity1
    Binding sitei231AspartateBy similarity1
    Binding sitei423AspartateBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionAminotransferase, Transferase
    LigandPyridoxal phosphate

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aspartate aminotransferase 3, chloroplastic (EC:2.6.1.1)
    Alternative name(s):
    Protein YELLOW-LEAF-SPECIFIC GENE 4
    Transaminase A
    Gene namesi
    Name:ASP3
    Synonyms:YLS4
    Ordered Locus Names:At5g11520
    ORF Names:F15N18.110
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 5

    Organism-specific databases

    AraportiAT5G11520.
    TAIRilocus:2144226. AT5G11520.

    Subcellular locationi

    • Plastidchloroplast 1 Publication

    • Note: accumulates in stromules, which are membrane-bound protrusions of the plastid envelope containing soluble stroma. Stromules are often found connecting plastids within a cell.

    GO - Cellular componenti

    • chloroplast Source: UniProtKB-SubCell
    • membrane Source: TAIR
    • peroxisome Source: TAIR
    • plastid Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transit peptidei1 – 43ChloroplastSequence analysisAdd BLAST43
    ChainiPRO_000000121044 – 449Aspartate aminotransferase 3, chloroplasticAdd BLAST406

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei295N6-(pyridoxal phosphate)lysineBy similarity1

    Proteomic databases

    PaxDbiP46644.
    PRIDEiP46644.

    Expressioni

    Tissue specificityi

    Expressed in roots, cauline leaves, flowers, hypocotyl epidermis and root hair cells.2 Publications

    Developmental stagei

    Up-regulated in leaves during natural senescence.1 Publication

    Inductioni

    By ethylene and dark.1 Publication

    Gene expression databases

    ExpressionAtlasiP46644. baseline and differential.
    GenevisibleiP46644. AT.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    BioGridi16302. 2 interactors.
    IntActiP46644. 1 interactor.
    STRINGi3702.AT5G11520.1.

    Structurei

    3D structure databases

    ProteinModelPortaliP46644.
    SMRiP46644.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiKOG1411. Eukaryota.
    COG1448. LUCA.
    HOGENOMiHOG000185898.
    InParanoidiP46644.
    KOiK14454.
    OMAiRVGACTI.
    OrthoDBiEOG09360C83.
    PhylomeDBiP46644.

    Family and domain databases

    Gene3Di3.90.1150.10. 2 hits.
    InterProiView protein in InterPro
    IPR004839. Aminotransferase_I/II.
    IPR000796. Asp_trans.
    IPR004838. NHTrfase_class1_PyrdxlP-BS.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015422. PyrdxlP-dep_Trfase_sub2.
    PANTHERiPTHR11879. PTHR11879. 1 hit.
    PfamiView protein in Pfam
    PF00155. Aminotran_1_2. 1 hit.
    PRINTSiPR00799. TRANSAMINASE.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiView protein in PROSITE
    PS00105. AA_TRANSFER_CLASS_1. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P46644-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKTTHFSSSS SSDRRIGALL RHLNSGSDSD NLSSLYASPT SGGTGGSVFS
    60 70 80 90 100
    HLVQAPEDPI LGVTVAYNKD PSPVKLNLGV GAYRTEEGKP LVLNVVRKAE
    110 120 130 140 150
    QQLINDRTRI KEYLPIVGLV EFNKLSAKLI LGADSPAIRE NRITTVECLS
    160 170 180 190 200
    GTGSLRVGGE FLAKHYHQKT IYITQPTWGN HPKIFTLAGL TVKTYRYYDP
    210 220 230 240 250
    ATRGLNFQGL LEDLGAAAPG SIVLLHACAH NPTGVDPTIQ QWEQIRKLMR
    260 270 280 290 300
    SKGLMPFFDS AYQGFASGSL DTDAKPIRMF VADGGECLVA QSYAKNMGLY
    310 320 330 340 350
    GERVGALSIV CKSADVAGRV ESQLKLVIRP MYSSPPIHGA SIVAVILRDK
    360 370 380 390 400
    NLFNEWTLEL KAMADRIISM RKQLFEALRT RGTPGDWSHI IKQIGMFTFT
    410 420 430 440
    GLNPAQVSFM TKEYHIYMTS DGRISMAGLS SKTVPHLADA IHAVVTKAV
    Length:449
    Mass (Da):48,954
    Last modified:November 1, 1995 - v1
    Checksum:i8668EA40367C6A58
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U15034 mRNA. Translation: AAA79371.1.
    AL163815 Genomic DNA. Translation: CAB87712.1.
    CP002688 Genomic DNA. Translation: AED91691.1.
    AY050765 mRNA. Translation: AAK92700.1.
    AY079310 mRNA. Translation: AAL85041.1.
    AB047807 mRNA. Translation: BAB32884.1.
    PIRiT48511.
    RefSeqiNP_196713.1. NM_121190.3.
    UniGeneiAt.21687.

    Genome annotation databases

    EnsemblPlantsiAT5G11520.1; AT5G11520.1; AT5G11520.
    GeneIDi831024.
    GrameneiAT5G11520.1; AT5G11520.1; AT5G11520.
    KEGGiath:AT5G11520.

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

    Entry informationi

    Entry nameiAAT3_ARATH
    AccessioniPrimary (citable) accession number: P46644
    Secondary accession number(s): Q9CAZ5
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: June 7, 2017
    This is version 139 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families