Reviewed, UniProtKB/Swiss-Prot P46643 (AAT1_ARATH)

Names and origin

Protein names

Recommended name:
    Aspartate aminotransferase, mitochondrial
    EC=2.6.1.1
Alternative name(s):
    Transaminase A

Gene names

Name:	ASP1
Ordered Locus Names:	At2g30970
ORF Names:	F7F1.18

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Important for the metabolism of amino acids and Krebs-cycle related organic acids. In plants, it is involved in nitrogen metabolism and in aspects of carbon and energy metabolism.
Catalytic activity	L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.
Cofactor	Pyridoxal phosphate.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion matrix. Ref.4
Miscellaneous	In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.
Sequence similarities	Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro response to cadmium ion Inferred from expression pattern. Source: TAIR
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-aspartate:2-oxoglutarate aminotransferase activity Inferred from direct assay. Source: TAIR pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 28	28	Mitochondrion Potential
	Chain	29 – 430	402	Aspartate aminotransferase, mitochondrial		PRO_0000001209
Amino acid modifications
	Modified residue	277	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P46643-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: A6525B958B0249EF	FASTA	430	47,758
	10 20 30 40 50 60 MALAMMIRNA ASKRGMTPIS GHFGGLRSMS SWWKSVEPAP KDPILGVTEA FLADPSPEKV 70 80 90 100 110 120 NVGVGAYRDD NGKPVVLECV REAEKRLAGS TFMEYLPMGG SAKMVDLTLK LAYGDNSEFI 130 140 150 160 170 180 KDKRIAAVQT LSGTGACRLF ADFQKRFSPG SQIYIPVPTW SNHHNIWKDA QVPQKTYHYY 190 200 210 220 230 240 HPETKGLDFS ALMDDVKNAP EGSFFLLHAC AHNPTGVDPT EEQWREISQL FKAKKHFAFF 250 260 270 280 290 300 DMAYQGFASG DPARDAKSIR IFLEDGHHIG ISQSYAKNMG LYGQRVGCLS VLCEDPKQAV 310 320 330 340 350 360 AVKSQLQQLA RPMYSNPPLH GAQLVSTILE DPELKSLWLK EVKVMADRII GMRTTLRESL 370 380 390 400 410 420 EKLGSPLSWE HVTKQIGMFC YSGLTPEQVD RLTSEYHIYM TRNGRISMAG VTTGNVGYLA 430 NAIHEVTKSS

References

[1]	"The aspartate aminotransferase gene family of Arabidopsis encodes isoenzymes localized to three distinct subcellular compartments." Schultz C.J., Coruzzi G.M. Plant J. 7:61-75(1995) [PubMed: 7894512] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Leaf.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins." Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H. Plant Cell 16:241-256(2004) [PubMed: 14671022] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U15026 mRNA. Translation: AAA79369.1. AC004669 Genomic DNA. Translation: AAC20731.1. AY059912 mRNA. Translation: AAL24394.1. AY128806 mRNA. Translation: AAM91206.1.
IPI	IPI00539251.
PIR	H84714.
RefSeq	NP_001118421.1. NP_180654.1.
UniGene	At.513
3D structure databases
HSSP	HSSP built from PDB template 7AAT based on UniProtKB P00508.
ModBase	Search...
Protein-protein interaction databases
STRING	P46643.
Proteomic databases
PRIDE	P46643.
Genome annotation databases
GeneID	817648.
GenomeReviews	Gene locus AT2G30970 in contig CT485783_GR.
KEGG	ath:AT2G30970.
NMPDR	fig|3702.1.peg.10156.
Organism-specific databases
GeneFarm	4377.
TAIR	At2g30970.
Phylogenomic databases
OMA	IKILIRP.
Enzyme and pathway databases
BRENDA	2.6.1.1. 302.
Gene expression databases
ArrayExpress	P46643.
Genevestigator	P46643.
GermOnline	AT2G30970. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR004839. Aminotransferase_I/II. IPR000796. Asp_trans. IPR004838. NHTrfase_class1_PyrdxlP-BS. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11879. Asp_trans. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
PRINTS	PR00799. TRANSAMINASE.
PROSITE	PS00105. AA_TRANSFER_CLASS_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AAT1_ARATH

Accession

Primary (citable) accession number: P46643

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 3, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)