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Protein

Homeobox protein knotted-1-like 1

Gene

KNAT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in meristem function, and may be involved in maintaining cells in an undifferentiated, meristematic state, and its expression disappears at the same time the shoot apex undergoes the transition from vegetative to reproductive development (PubMed:11934861). Positive regulator of LATERAL ORGAN BOUNDARIES (LOB) (PubMed:11934861). Probably binds to the DNA sequence 5'-TGAC-3' (PubMed:11934861). Able to traffic from the L1 to the L2/L3 layers of the meristem, presumably through plasmodesmata (PubMed:12900451).3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi300 – 36364Homeobox; TALE-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • cell fate commitment Source: TAIR
  • cell fate specification Source: TAIR
  • xylem and phloem pattern formation Source: TAIR
  • xylem development Source: TAIR
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein knotted-1-like 1
Alternative name(s):
Protein BREVIPEDICELLUS
Protein KNAT1
Gene namesi
Name:KNAT1
Synonyms:BP, BP1
Ordered Locus Names:At4g08150
ORF Names:F9M13.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G08150.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 398398Homeobox protein knotted-1-like 1PRO_0000048957Add
BLAST

Proteomic databases

PaxDbiP46639.
PRIDEiP46639.

Expressioni

Tissue specificityi

Expressed in the vegetative meristem.2 Publications

Inductioni

Negatively regulated by ASYMMETRIC LEAVES1 (AS1) and ASYMMETRIC LEAVES2 (AS2).

Gene expression databases

GenevisibleiP46639. AT.

Interactioni

Subunit structurei

May form heterodimeric complex with the TALE/BELL proteins BEL1, BLH2, BLH8/PNF and BLH9/PNY (PubMed:11701881, PubMed:12897247, PubMed:16741748, PubMed:17873098). Interacts with OFP1, OFP2, OFP4, OFP6 and OFP12 (PubMed:15781858). Interacts with CCT7 and CCT8 (PubMed:21868675). Interacts with KNATM-B (PubMed:18398054).7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BEL1Q388976EBI-530486,EBI-1153783
BLH9Q9LZM84EBI-530486,EBI-530473

Protein-protein interaction databases

BioGridi11664. 44 interactions.
IntActiP46639. 40 interactions.
STRINGi3702.AT4G08150.1.

Structurei

3D structure databases

ProteinModelPortaliP46639.
SMRiP46639. Positions 300-358.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini279 – 29921ELKPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi35 – 428Poly-Asn
Compositional biasi51 – 577Poly-Asn
Compositional biasi88 – 925Poly-Asn
Compositional biasi121 – 1277Poly-Asn
Compositional biasi301 – 3077Poly-Lys

Sequence similaritiesi

Belongs to the TALE/KNOX homeobox family.PROSITE-ProRule annotation
Contains 1 ELK domain.PROSITE-ProRule annotation
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0773. Eukaryota.
ENOG410XPMQ. LUCA.
HOGENOMiHOG000272741.
InParanoidiP46639.
OMAiRENDPPE.
PhylomeDBiP46639.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR005539. ELK_dom.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR008422. Homeobox_KN_domain.
IPR009057. Homeodomain-like.
IPR005540. KNOX1.
IPR005541. KNOX2.
[Graphical view]
PfamiPF03789. ELK. 1 hit.
PF05920. Homeobox_KN. 1 hit.
PF03790. KNOX1. 1 hit.
PF03791. KNOX2. 1 hit.
[Graphical view]
SMARTiSM01188. ELK. 1 hit.
SM00389. HOX. 1 hit.
SM01255. KNOX1. 1 hit.
SM01256. KNOX2. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS51213. ELK. 1 hit.
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46639-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEYQHDNST TPQRVSFLYS PISSSNKNDN TSDTNNNNNN NNSSNYGPGY
60 70 80 90 100
NNTNNNNHHH QHMLFPHMSS LLPQTTENCF RSDHDQPNNN NNPSVKSEAS
110 120 130 140 150
SSRINHYSML MRAIHNTQEA NNNNNDNVSD VEAMKAKIIA HPHYSTLLQA
160 170 180 190 200
YLDCQKIGAP PDVVDRITAA RQDFEARQQR STPSVSASSR DPELDQFMEA
210 220 230 240 250
YCDMLVKYRE ELTRPIQEAM EFIRRIESQL SMLCQSPIHI LNNPDGKSDN
260 270 280 290 300
MGSSDEEQEN NSGGETELPE IDPRAEDREL KNHLLKKYSG YLSSLKQELS
310 320 330 340 350
KKKKKGKLPK EARQKLLTWW ELHYKWPYPS ESEKVALAES TGLDQKQINN
360 370 380 390
WFINQRKRHW KPSEDMQFMV MDGLQHPHHA ALYMDGHYMG DGPYRLGP
Length:398
Mass (Da):45,836
Last modified:November 1, 1995 - v1
Checksum:i815A22786589A316
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti10 – 101T → S in AAM03027 (PubMed:11917137).Curated
Sequence conflicti10 – 101T → S in AAM03026 (PubMed:11917137).Curated
Sequence conflicti77 – 771E → D in AAM03026 (PubMed:11917137).Curated
Sequence conflicti92 – 921N → NN in AAM03027 (PubMed:11917137).Curated
Sequence conflicti92 – 921N → NN in AAM03026 (PubMed:11917137).Curated
Sequence conflicti125 – 1251N → NN in AAM03027 (PubMed:11917137).Curated
Sequence conflicti125 – 1251N → NN in AAM03026 (PubMed:11917137).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14174 Genomic DNA. Translation: AAA67881.1.
AF482994 mRNA. Translation: AAM03026.1.
AF482995 mRNA. Translation: AAM03027.1.
AC006267 Genomic DNA. Translation: AAD27897.1.
AL161510 Genomic DNA. Translation: CAB81151.1.
CP002687 Genomic DNA. Translation: AEE82597.1.
AY080834 mRNA. Translation: AAL87309.1.
AY113982 mRNA. Translation: AAM45030.1.
PIRiD85080.
RefSeqiNP_192555.1. NM_116884.3.
UniGeneiAt.28060.
At.72028.

Genome annotation databases

EnsemblPlantsiAT4G08150.1; AT4G08150.1; AT4G08150.
GeneIDi826364.
GrameneiAT4G08150.1; AT4G08150.1; AT4G08150.
KEGGiath:AT4G08150.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14174 Genomic DNA. Translation: AAA67881.1.
AF482994 mRNA. Translation: AAM03026.1.
AF482995 mRNA. Translation: AAM03027.1.
AC006267 Genomic DNA. Translation: AAD27897.1.
AL161510 Genomic DNA. Translation: CAB81151.1.
CP002687 Genomic DNA. Translation: AEE82597.1.
AY080834 mRNA. Translation: AAL87309.1.
AY113982 mRNA. Translation: AAM45030.1.
PIRiD85080.
RefSeqiNP_192555.1. NM_116884.3.
UniGeneiAt.28060.
At.72028.

3D structure databases

ProteinModelPortaliP46639.
SMRiP46639. Positions 300-358.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi11664. 44 interactions.
IntActiP46639. 40 interactions.
STRINGi3702.AT4G08150.1.

Proteomic databases

PaxDbiP46639.
PRIDEiP46639.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G08150.1; AT4G08150.1; AT4G08150.
GeneIDi826364.
GrameneiAT4G08150.1; AT4G08150.1; AT4G08150.
KEGGiath:AT4G08150.

Organism-specific databases

TAIRiAT4G08150.

Phylogenomic databases

eggNOGiKOG0773. Eukaryota.
ENOG410XPMQ. LUCA.
HOGENOMiHOG000272741.
InParanoidiP46639.
OMAiRENDPPE.
PhylomeDBiP46639.

Miscellaneous databases

PROiP46639.

Gene expression databases

GenevisibleiP46639. AT.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR005539. ELK_dom.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR008422. Homeobox_KN_domain.
IPR009057. Homeodomain-like.
IPR005540. KNOX1.
IPR005541. KNOX2.
[Graphical view]
PfamiPF03789. ELK. 1 hit.
PF05920. Homeobox_KN. 1 hit.
PF03790. KNOX1. 1 hit.
PF03791. KNOX2. 1 hit.
[Graphical view]
SMARTiSM01188. ELK. 1 hit.
SM00389. HOX. 1 hit.
SM01255. KNOX1. 1 hit.
SM01256. KNOX2. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS51213. ELK. 1 hit.
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A knotted1-like homeobox gene in Arabidopsis is expressed in the vegetative meristem and dramatically alters leaf morphology when overexpressed in transgenic plants."
    Lincoln C., Long J., Yamaguchi J., Serikawa K.A., Hake S.
    Plant Cell 6:1859-1876(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, FUNCTION.
    Strain: cv. Columbia.
  2. "The homeobox gene BREVIPEDICELLUS is a key regulator of inflorescence architecture in Arabidopsis."
    Venglat S.P., Dumonceaux T., Rozwadowski K., Parnell L., Babic V., Keller W., Martienssen R., Selvaraj G., Datla R.
    Proc. Natl. Acad. Sci. U.S.A. 99:4730-4735(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Landsberg erecta and cv. RLD.
  3. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "The Arabidopsis BELL1 and KNOX TALE homeodomain proteins interact through a domain conserved between plants and animals."
    Bellaoui M., Pidkowich M.S., Samach A., Kushalappa K., Kohalmi S.E., Modrusan Z., Crosby W.L., Haughn G.W.
    Plant Cell 13:2455-2470(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BEL1.
  7. "ASYMMETRIC LEAVES1 reveals knox gene redundancy in Arabidopsis."
    Byrne M.E., Simorowski J., Martienssen R.A.
    Development 129:1957-1965(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, REGULATION.
  8. "Developmental regulation and significance of KNOX protein trafficking in Arabidopsis."
    Kim J.Y., Yuan Z., Jackson D.
    Development 130:4351-4362(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  9. "The interaction of two homeobox genes, BREVIPEDICELLUS and PENNYWISE, regulates internode patterning in the Arabidopsis inflorescence."
    Smith H.M.S., Hake S.
    Plant Cell 15:1717-1727(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BLH9/PNY.
  10. "A central role of Arabidopsis thaliana ovate family proteins in networking and subcellular localization of 3-aa loop extension homeodomain proteins."
    Hackbusch J., Richter K., Muller J., Salamini F., Uhrig J.F.
    Proc. Natl. Acad. Sci. U.S.A. 102:4908-4912(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH OFP1; OFP2; OFP4; OFP6 AND OFP12.
  11. "Arabidopsis inflorescence architecture requires the activities of KNOX-BELL homeodomain heterodimers."
    Kanrar S., Onguka O., Smith H.M.S.
    Planta 224:1163-1173(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BLH8/PNF AND BLH9/PNY.
  12. "The Arabidopsis BEL1-LIKE HOMEODOMAIN proteins SAW1 and SAW2 act redundantly to regulate KNOX expression spatially in leaf margins."
    Kumar R., Kushalappa K., Godt D., Pidkowich M.S., Pastorelli S., Hepworth S.R., Haughn G.W.
    Plant Cell 19:2719-2735(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BEL1 AND BLH2.
  13. "KNOX lost the OX: the Arabidopsis KNATM gene defines a novel class of KNOX transcriptional regulators missing the homeodomain."
    Magnani E., Hake S.
    Plant Cell 20:875-887(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KNATM.
  14. "Chaperonins facilitate KNOTTED1 cell-to-cell trafficking and stem cell function."
    Xu X.M., Wang J., Xuan Z., Goldshmidt A., Borrill P.G., Hariharan N., Kim J.Y., Jackson D.
    Science 333:1141-1144(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CCT7 AND CCT8.

Entry informationi

Entry nameiKNAT1_ARATH
AccessioniPrimary (citable) accession number: P46639
Secondary accession number(s): Q8S3L9, Q8S3M0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 11, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.