Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Arginase 1, mitochondrial

Gene

ARGAH1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of L-arginine to urea and L-ornithine. The latter can be utilized in the urea cycle or as a precursor for the synthesis of both polyamines and proline. Possesses agmatinase activity. Catalyzes the formation of putrescine from agmatine (PubMed:28716421).By similarity1 Publication

Catalytic activityi

L-arginine + H2O = L-ornithine + urea.By similarity
Agmatine + H2O = putrescine + urea.1 Publication

Cofactori

Mn2+PROSITE-ProRule annotationNote: Binds 2 manganese ions per subunit.PROSITE-ProRule annotation

Pathwayi: urea cycle

This protein is involved in step 1 of the subpathway that synthesizes L-ornithine and urea from L-arginine.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. Arginase 1, mitochondrial (ARGAH1), Arginase 2, chloroplastic/mitochondrial (ARGAH2)
This subpathway is part of the pathway urea cycle, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-ornithine and urea from L-arginine, the pathway urea cycle and in Nitrogen metabolism.

Pathwayi: putrescine biosynthesis via agmatine pathway

This protein is involved in step 1 of the subpathway that synthesizes putrescine from agmatine.1 Publication
Proteins known to be involved in this subpathway in this organism are:
  1. Arginase 1, mitochondrial (ARGAH1), Arginase 2, chloroplastic/mitochondrial (ARGAH2)
This subpathway is part of the pathway putrescine biosynthesis via agmatine pathway, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes putrescine from agmatine, the pathway putrescine biosynthesis via agmatine pathway and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi161Manganese 1PROSITE-ProRule annotation1
Metal bindingi185Manganese 1PROSITE-ProRule annotation1
Metal bindingi185Manganese 2PROSITE-ProRule annotation1
Metal bindingi187Manganese 2PROSITE-ProRule annotation1
Metal bindingi189Manganese 1PROSITE-ProRule annotation1
Binding sitei226SubstrateBy similarity1
Metal bindingi270Manganese 1PROSITE-ProRule annotation1
Metal bindingi270Manganese 2PROSITE-ProRule annotation1
Metal bindingi272Manganese 2PROSITE-ProRule annotation1
Binding sitei313SubstrateBy similarity1

GO - Molecular functioni

  • agmatinase activity Source: UniProtKB
  • arginase activity Source: TAIR
  • cobalt ion binding Source: TAIR

GO - Biological processi

  • arginine catabolic process Source: TAIR
  • defense response to bacterium Source: TAIR
  • putrescine biosynthetic process from arginine, using agmatinase Source: UniProtKB
  • urea cycle Source: UniProtKB-UniPathway

Keywordsi

Molecular functionHydrolase
Biological processArginine metabolism, Putrescine biosynthesis
LigandManganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT4G08900-MONOMER
MetaCyc:AT4G08900-MONOMER
ReactomeiR-ATH-351143 Agmatine biosynthesis
UniPathwayiUPA00158; UER00270
UPA00534; UER00287

Names & Taxonomyi

Protein namesi
Recommended name:
Arginase 1, mitochondrial (EC:3.5.3.1By similarity)
Alternative name(s):
Agmatinase ARGAH1Curated (EC:3.5.3.111 Publication)
Arginine amidohydrolase 1
Gene namesi
Name:ARGAH1
Ordered Locus Names:At4g08900
ORF Names:T3H13.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G08900
TAIRilocus:2138718 AT4G08900

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Increased formation of lateral and adventitious roots and increased production of NO in roots.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 22Mitochondrion1 PublicationAdd BLAST22
ChainiPRO_000017370323 – 342Arginase 1, mitochondrialAdd BLAST320

Proteomic databases

PaxDbiP46637

Expressioni

Tissue specificityi

Expressed in vasculature of roots, root tips, cotyledons, leaves, cauline leaves, stems, sepals and pollen.2 Publications

Gene expression databases

ExpressionAtlasiP46637 baseline and differential
GenevisibleiP46637 AT

Interactioni

Protein-protein interaction databases

BioGridi11767, 2 interactors
IntActiP46637, 1 interactor
STRINGi3702.AT4G08900.1

Structurei

3D structure databases

ProteinModelPortaliP46637
SMRiP46637
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni187 – 191Substrate bindingBy similarity5
Regioni195 – 197Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the arginase family.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2964 Eukaryota
COG0010 LUCA
HOGENOMiHOG000204320
InParanoidiP46637
KOiK01476
OMAiCIDAGFV
OrthoDBiEOG09360FUJ
PhylomeDBiP46637

Family and domain databases

InterProiView protein in InterPro
IPR006035 Ureohydrolase
IPR023696 Ureohydrolase_dom_sf
IPR020855 Ureohydrolase_Mn_BS
PfamiView protein in Pfam
PF00491 Arginase, 1 hit
PIRSFiPIRSF036979 Arginase, 1 hit
SUPFAMiSSF52768 SSF52768, 1 hit
PROSITEiView protein in PROSITE
PS01053 ARGINASE_1, 1 hit
PS51409 ARGINASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46637-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRIIGRKGI NYIHRLNSAS FTSVSASSIE KGQNRVIDAS LTLIRERAKL
60 70 80 90 100
KGELVRLLGG AKASTSLLGV PLGHNSSFLQ GPAFAPPRIR EAIWCGSTNS
110 120 130 140 150
ATEEGKELKD PRVLTDVGDV PVQEIRDCGV DDDRLMNVIS ESVKLVMEEE
160 170 180 190 200
PLRPLVLGGD HSISYPVVRA VSEKLGGPVD ILHLDAHPDI YDCFEGNKYS
210 220 230 240 250
HASSFARIME GGYARRLLQV GIRSINQEGR EQGKRFGVEQ YEMRTFSKDR
260 270 280 290 300
PMLENLKLGE GVKGVYISID VDCLDPAFAP GVSHIEPGGL SFRDVLNILH
310 320 330 340
NLQADVVGAD VVEFNPQRDT VDGMTAMVAA KLVRELAAKI SK
Length:342
Mass (Da):37,345
Last modified:November 1, 1995 - v1
Checksum:iB656635BB51DCD3F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15019 mRNA Translation: AAA85816.1
AF128396 Genomic DNA Translation: AAD17369.1
AL161513 Genomic DNA Translation: CAB78014.1
CP002687 Genomic DNA Translation: AEE82694.1
AY052276 mRNA Translation: AAK96469.1
AY061914 mRNA Translation: AAL31241.1
PIRiF85089
RefSeqiNP_192629.1, NM_116959.4
UniGeneiAt.1554

Genome annotation databases

EnsemblPlantsiAT4G08900.1; AT4G08900.1; AT4G08900
GeneIDi826468
GrameneiAT4G08900.1; AT4G08900.1; AT4G08900
KEGGiath:AT4G08900

Similar proteinsi

Entry informationi

Entry nameiARGI1_ARATH
AccessioniPrimary (citable) accession number: P46637
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 25, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health