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Protein

Arginase 1, mitochondrial

Gene

ARGAH1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of L-arginine to urea and L-ornithine. The latter can be utilized in the urea cycle or as a precursor for the synthesis of both polyamines and proline (By similarity).By similarity

Catalytic activityi

L-arginine + H2O = L-ornithine + urea.By similarity

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Pathwayi: urea cycle

This protein is involved in step 1 of the subpathway that synthesizes L-ornithine and urea from L-arginine.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. Arginase 1, mitochondrial (ARGAH1), Arginase 2, mitochondrial (ARGAH2)
This subpathway is part of the pathway urea cycle, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-ornithine and urea from L-arginine, the pathway urea cycle and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi161Manganese 1PROSITE-ProRule annotation1
Metal bindingi185Manganese 1PROSITE-ProRule annotation1
Metal bindingi185Manganese 2PROSITE-ProRule annotation1
Metal bindingi187Manganese 2PROSITE-ProRule annotation1
Metal bindingi189Manganese 1PROSITE-ProRule annotation1
Binding sitei226SubstrateBy similarity1
Metal bindingi270Manganese 1PROSITE-ProRule annotation1
Metal bindingi270Manganese 2PROSITE-ProRule annotation1
Metal bindingi272Manganese 2PROSITE-ProRule annotation1
Binding sitei313SubstrateBy similarity1

GO - Molecular functioni

  • arginase activity Source: TAIR
  • cobalt ion binding Source: TAIR

GO - Biological processi

  • arginine catabolic process Source: TAIR
  • defense response to bacterium Source: TAIR
  • urea cycle Source: UniProtKB-UniPathway

Keywordsi

Molecular functionHydrolase
Biological processArginine metabolism
LigandManganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT4G08900-MONOMER.
MetaCyc:AT4G08900-MONOMER.
ReactomeiR-ATH-351143. Agmatine biosynthesis.
UniPathwayiUPA00158; UER00270.

Names & Taxonomyi

Protein namesi
Recommended name:
Arginase 1, mitochondrial (EC:3.5.3.1By similarity)
Alternative name(s):
Arginine amidohydrolase 1
Gene namesi
Name:ARGAH1
Ordered Locus Names:At4g08900
ORF Names:T3H13.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G08900.
TAIRilocus:2138718. AT4G08900.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Increased formation of lateral and adventitious roots and increased production of NO in roots.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 22Mitochondrion1 PublicationAdd BLAST22
ChainiPRO_000017370323 – 342Arginase 1, mitochondrialAdd BLAST320

Proteomic databases

PaxDbiP46637.

Expressioni

Tissue specificityi

Expressed in vasculature of roots, root tips, cotyledons, leaves, cauline leaves, stems, sepals and pollen.2 Publications

Gene expression databases

ExpressionAtlasiP46637. baseline and differential.
GenevisibleiP46637. AT.

Interactioni

Protein-protein interaction databases

BioGridi11767. 2 interactors.
IntActiP46637. 1 interactor.
STRINGi3702.AT4G08900.1.

Structurei

3D structure databases

ProteinModelPortaliP46637.
SMRiP46637.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni187 – 191Substrate bindingBy similarity5
Regioni195 – 197Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the arginase family.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2964. Eukaryota.
COG0010. LUCA.
HOGENOMiHOG000204320.
InParanoidiP46637.
KOiK01476.
OMAiCIDAGFV.
OrthoDBiEOG09360FUJ.
PhylomeDBiP46637.

Family and domain databases

Gene3Di3.40.800.10. 1 hit.
InterProiView protein in InterPro
IPR006035. Ureohydrolase.
IPR023696. Ureohydrolase_domain.
IPR020855. Ureohydrolase_Mn_BS.
PANTHERiPTHR11358. PTHR11358. 1 hit.
PfamiView protein in Pfam
PF00491. Arginase. 1 hit.
PIRSFiPIRSF036979. Arginase. 1 hit.
SUPFAMiSSF52768. SSF52768. 1 hit.
PROSITEiView protein in PROSITE
PS01053. ARGINASE_1. 1 hit.
PS51409. ARGINASE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46637-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRIIGRKGI NYIHRLNSAS FTSVSASSIE KGQNRVIDAS LTLIRERAKL
60 70 80 90 100
KGELVRLLGG AKASTSLLGV PLGHNSSFLQ GPAFAPPRIR EAIWCGSTNS
110 120 130 140 150
ATEEGKELKD PRVLTDVGDV PVQEIRDCGV DDDRLMNVIS ESVKLVMEEE
160 170 180 190 200
PLRPLVLGGD HSISYPVVRA VSEKLGGPVD ILHLDAHPDI YDCFEGNKYS
210 220 230 240 250
HASSFARIME GGYARRLLQV GIRSINQEGR EQGKRFGVEQ YEMRTFSKDR
260 270 280 290 300
PMLENLKLGE GVKGVYISID VDCLDPAFAP GVSHIEPGGL SFRDVLNILH
310 320 330 340
NLQADVVGAD VVEFNPQRDT VDGMTAMVAA KLVRELAAKI SK
Length:342
Mass (Da):37,345
Last modified:November 1, 1995 - v1
Checksum:iB656635BB51DCD3F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15019 mRNA. Translation: AAA85816.1.
AF128396 Genomic DNA. Translation: AAD17369.1.
AL161513 Genomic DNA. Translation: CAB78014.1.
CP002687 Genomic DNA. Translation: AEE82694.1.
AY052276 mRNA. Translation: AAK96469.1.
AY061914 mRNA. Translation: AAL31241.1.
PIRiF85089.
RefSeqiNP_192629.1. NM_116959.4.
UniGeneiAt.1554.

Genome annotation databases

EnsemblPlantsiAT4G08900.1; AT4G08900.1; AT4G08900.
GeneIDi826468.
GrameneiAT4G08900.1; AT4G08900.1; AT4G08900.
KEGGiath:AT4G08900.

Similar proteinsi

Entry informationi

Entry nameiARGI1_ARATH
AccessioniPrimary (citable) accession number: P46637
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 25, 2017
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families