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Protein

Arginase 1, mitochondrial

Gene

ARGAH1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the hydrolysis of L-arginine to urea and L-ornithine. The latter can be utilized in the urea cycle or as a precursor for the synthesis of both polyamines and proline (By similarity).By similarity

Catalytic activityi

L-arginine + H2O = L-ornithine + urea.By similarity

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Pathwayi: urea cycle

This protein is involved in step 1 of the subpathway that synthesizes L-ornithine and urea from L-arginine.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. Arginase 1, mitochondrial (ARGAH1), Arginase 2, mitochondrial (ARGAH2)
This subpathway is part of the pathway urea cycle, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-ornithine and urea from L-arginine, the pathway urea cycle and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi161 – 1611Manganese 1PROSITE-ProRule annotation
Metal bindingi185 – 1851Manganese 1PROSITE-ProRule annotation
Metal bindingi185 – 1851Manganese 2PROSITE-ProRule annotation
Metal bindingi187 – 1871Manganese 2PROSITE-ProRule annotation
Metal bindingi189 – 1891Manganese 1PROSITE-ProRule annotation
Binding sitei226 – 2261SubstrateBy similarity
Metal bindingi270 – 2701Manganese 1PROSITE-ProRule annotation
Metal bindingi270 – 2701Manganese 2PROSITE-ProRule annotation
Metal bindingi272 – 2721Manganese 2PROSITE-ProRule annotation
Binding sitei313 – 3131SubstrateBy similarity

GO - Molecular functioni

  • arginase activity Source: TAIR
  • cobalt ion binding Source: TAIR

GO - Biological processi

  • arginine catabolic process Source: TAIR
  • defense response to bacterium Source: TAIR
  • urea cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Arginine metabolism

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT4G08900-MONOMER.
MetaCyc:AT4G08900-MONOMER.
ReactomeiR-ATH-351143. Agmatine biosynthesis.
UniPathwayiUPA00158; UER00270.

Names & Taxonomyi

Protein namesi
Recommended name:
Arginase 1, mitochondrial (EC:3.5.3.1By similarity)
Alternative name(s):
Arginine amidohydrolase 1
Gene namesi
Name:ARGAH1
Ordered Locus Names:At4g08900
ORF Names:T3H13.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G08900.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Increased formation of lateral and adventitious roots and increased production of NO in roots.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2424MitochondrionSequence analysisAdd
BLAST
Chaini25 – 342318Arginase 1, mitochondrialPRO_0000173703Add
BLAST

Proteomic databases

PaxDbiP46637.
PRIDEiP46637.

Expressioni

Tissue specificityi

Expressed in vasculature of roots, root tips, cotyledons, leaves, cauline leaves, stems, sepals and pollen.2 Publications

Gene expression databases

GenevisibleiP46637. AT.

Interactioni

Protein-protein interaction databases

BioGridi11767. 2 interactions.
IntActiP46637. 1 interaction.
STRINGi3702.AT4G08900.1.

Structurei

3D structure databases

ProteinModelPortaliP46637.
SMRiP46637. Positions 66-336.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni187 – 1915Substrate bindingBy similarity
Regioni195 – 1973Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the arginase family.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2964. Eukaryota.
COG0010. LUCA.
HOGENOMiHOG000204320.
InParanoidiP46637.
KOiK01476.
OMAiVHIDAHF.
OrthoDBiEOG09360FUJ.
PhylomeDBiP46637.

Family and domain databases

Gene3Di3.40.800.10. 1 hit.
InterProiIPR006035. Ureohydrolase.
IPR023696. Ureohydrolase_domain.
IPR020855. Ureohydrolase_Mn_BS.
[Graphical view]
PANTHERiPTHR11358. PTHR11358. 1 hit.
PfamiPF00491. Arginase. 1 hit.
[Graphical view]
PIRSFiPIRSF036979. Arginase. 1 hit.
PROSITEiPS01053. ARGINASE_1. 1 hit.
PS51409. ARGINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46637-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRIIGRKGI NYIHRLNSAS FTSVSASSIE KGQNRVIDAS LTLIRERAKL
60 70 80 90 100
KGELVRLLGG AKASTSLLGV PLGHNSSFLQ GPAFAPPRIR EAIWCGSTNS
110 120 130 140 150
ATEEGKELKD PRVLTDVGDV PVQEIRDCGV DDDRLMNVIS ESVKLVMEEE
160 170 180 190 200
PLRPLVLGGD HSISYPVVRA VSEKLGGPVD ILHLDAHPDI YDCFEGNKYS
210 220 230 240 250
HASSFARIME GGYARRLLQV GIRSINQEGR EQGKRFGVEQ YEMRTFSKDR
260 270 280 290 300
PMLENLKLGE GVKGVYISID VDCLDPAFAP GVSHIEPGGL SFRDVLNILH
310 320 330 340
NLQADVVGAD VVEFNPQRDT VDGMTAMVAA KLVRELAAKI SK
Length:342
Mass (Da):37,345
Last modified:November 1, 1995 - v1
Checksum:iB656635BB51DCD3F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15019 mRNA. Translation: AAA85816.1.
AF128396 Genomic DNA. Translation: AAD17369.1.
AL161513 Genomic DNA. Translation: CAB78014.1.
CP002687 Genomic DNA. Translation: AEE82694.1.
AY052276 mRNA. Translation: AAK96469.1.
AY061914 mRNA. Translation: AAL31241.1.
PIRiF85089.
RefSeqiNP_192629.1. NM_116959.3.
UniGeneiAt.1554.

Genome annotation databases

EnsemblPlantsiAT4G08900.1; AT4G08900.1; AT4G08900.
GeneIDi826468.
GrameneiAT4G08900.1; AT4G08900.1; AT4G08900.
KEGGiath:AT4G08900.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15019 mRNA. Translation: AAA85816.1.
AF128396 Genomic DNA. Translation: AAD17369.1.
AL161513 Genomic DNA. Translation: CAB78014.1.
CP002687 Genomic DNA. Translation: AEE82694.1.
AY052276 mRNA. Translation: AAK96469.1.
AY061914 mRNA. Translation: AAL31241.1.
PIRiF85089.
RefSeqiNP_192629.1. NM_116959.3.
UniGeneiAt.1554.

3D structure databases

ProteinModelPortaliP46637.
SMRiP46637. Positions 66-336.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi11767. 2 interactions.
IntActiP46637. 1 interaction.
STRINGi3702.AT4G08900.1.

Proteomic databases

PaxDbiP46637.
PRIDEiP46637.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G08900.1; AT4G08900.1; AT4G08900.
GeneIDi826468.
GrameneiAT4G08900.1; AT4G08900.1; AT4G08900.
KEGGiath:AT4G08900.

Organism-specific databases

TAIRiAT4G08900.

Phylogenomic databases

eggNOGiKOG2964. Eukaryota.
COG0010. LUCA.
HOGENOMiHOG000204320.
InParanoidiP46637.
KOiK01476.
OMAiVHIDAHF.
OrthoDBiEOG09360FUJ.
PhylomeDBiP46637.

Enzyme and pathway databases

UniPathwayiUPA00158; UER00270.
BioCyciARA:AT4G08900-MONOMER.
MetaCyc:AT4G08900-MONOMER.
ReactomeiR-ATH-351143. Agmatine biosynthesis.

Miscellaneous databases

PROiP46637.

Gene expression databases

GenevisibleiP46637. AT.

Family and domain databases

Gene3Di3.40.800.10. 1 hit.
InterProiIPR006035. Ureohydrolase.
IPR023696. Ureohydrolase_domain.
IPR020855. Ureohydrolase_Mn_BS.
[Graphical view]
PANTHERiPTHR11358. PTHR11358. 1 hit.
PfamiPF00491. Arginase. 1 hit.
[Graphical view]
PIRSFiPIRSF036979. Arginase. 1 hit.
PROSITEiPS01053. ARGINASE_1. 1 hit.
PS51409. ARGINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARGI1_ARATH
AccessioniPrimary (citable) accession number: P46637
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.