P46634 (CP7A1_CRIGR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cholesterol 7-alpha-monooxygenase
EC=1.14.13.17

Alternative name(s):
CYPVII
Cholesterol 7-alpha-hydroxylase
Cytochrome P450 7A1

Gene names

Name:	CYP7A1
Synonyms:	CYP7

Organism

Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)

Taxonomic identifier

10029 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Cricetulus

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis By similarity.
Catalytic activity	Cholesterol + NADPH + O2 = 7-alpha-hydroxycholesterol + NADP+ + H2O.
Cofactor	Heme group By similarity.
Pathway	Lipid metabolism; bile acid biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Cholesterol metabolism Lipid metabolism Steroid metabolism
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	bile acid biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB cellular response to cholesterol Inferred from sequence or structural similarity. Source: UniProtKB cellular response to glucose stimulus Inferred from sequence or structural similarity. Source: UniProtKB cholesterol catabolic process Inferred from sequence or structural similarity. Source: UniProtKB cholesterol homeostasis Inferred from sequence or structural similarity. Source: UniProtKB regulation of bile acid biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	cholesterol 7-alpha-monooxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 504	504	Cholesterol 7-alpha-monooxygenase		PRO_0000051900
Sites
	Metal binding	444	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P46634 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: BD5E521D0C195257	FASTA	504	57,447
	10 20 30 40 50 60 MMTISLIWGI AMVVCCCIWV IFDRRRRKAG EPPLENGLIP YLGCALKFGS NPLEFLRANQ 70 80 90 100 110 120 RKHGHVFTCK LMGKYVHFIT NSLSYHKVLC HGKYFDWKKF HYTTSAKAFG HRSIDPNDGN 130 140 150 160 170 180 TTENINNTFT KTLQGDALHS LSEAMMQNLQ FVLRPPDLPK SKSDAWVTEG MYAFCYRVMF 190 200 210 220 230 240 EAGYLTLFGR DTSKPDTQRV LILNNLNSFK QFDQVFPALV AGLPIHLFKA AHKAREQLAE 250 260 270 280 290 300 GLKHENLSVR DQVSELIRLR MFLNDTLSTF DDMEKAKTHL AILWASQANT IPATFWSLFQ 310 320 330 340 350 360 MIRSPDALRA ASEEVNGALQ SAGQKLSSEG NAIYLDQIQL NNLPVLDSII KEALRLSSAS 370 380 390 400 410 420 LNIRTAKEDF TLHLEDGSYN IRKDDIIALY PQLMHLDPAI YPDPLTFKYD RYLDENKKAK 430 440 450 460 470 480 TSFYSNGNKL KYFYMPFGSG ATICPGRLFA VQEIKQFLIL MLSYFELELV ESHVKCPPLD 490 500 QSRAGLGILP PLNDIEFKYK LKHL

References

[1]	"Genomic cloning, sequencing, and analysis of the hamster cholesterol 7 alpha-hydroxylase gene (CYP7)." Crestani M., Galli G., Chiang J.Y. Arch. Biochem. Biophys. 306:451-460(1993) [PubMed: 8105753] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L04690 Genomic DNA. Translation: AAA03751.1.
PIR	S39399.
RefSeq	NP_001231330.1. NM_001244401.1.
3D structure databases
ProteinModelPortal	P46634.
SMR	P46634. Positions 25-503.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100689275.
Phylogenomic databases
HOVERGEN	HBG051100.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR024204. Cyt_P450_CYP7A1-type. IPR002403. Cyt_P450_E_grp-IV. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PIRSF	PIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
PRINTS	PR00465. EP450IV.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP7A1_CRIGR

Accession

Primary (citable) accession number: P46634

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program