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P46633

- HS90A_CRIGR

UniProt

P46633 - HS90A_CRIGR

Protein

Heat shock protein HSP 90-alpha

Gene

HSP90AA1

Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function By similarity. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei51 – 511ATPBy similarity
    Binding sitei93 – 931ATPBy similarity
    Binding sitei112 – 1121ATPBy similarity
    Binding sitei138 – 1381ATP; via amide nitrogenBy similarity
    Binding sitei401 – 4011ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. nitric-oxide synthase regulator activity Source: UniProtKB
    3. TPR domain binding Source: UniProtKB

    GO - Biological processi

    1. positive regulation of nitric oxide biosynthetic process Source: UniProtKB
    2. protein folding Source: InterPro
    3. regulation of catalytic activity Source: GOC
    4. response to stress Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    Stress response

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Heat shock protein HSP 90-alpha
    Gene namesi
    Name:HSP90AA1
    Synonyms:HSP90A, HSPCA
    OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
    Taxonomic identifieri10029 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

    Subcellular locationi

    Cytoplasm By similarity. Melanosome By similarity. Cell membrane By similarity

    GO - Cellular componenti

    1. melanosome Source: UniProtKB-SubCell
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 733732Heat shock protein HSP 90-alphaPRO_0000062909Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei5 – 51Phosphothreonine; by PRKDCBy similarity
    Modified residuei7 – 71Phosphothreonine; by PRKDCBy similarity
    Modified residuei58 – 581N6-acetyllysineBy similarity
    Modified residuei84 – 841N6-acetyllysineBy similarity
    Modified residuei231 – 2311PhosphoserineBy similarity
    Modified residuei263 – 2631PhosphoserineBy similarity
    Modified residuei314 – 3141PhosphotyrosineBy similarity
    Modified residuei400 – 4001PhosphoserineBy similarity
    Modified residuei444 – 4441N6-acetyllysineBy similarity
    Modified residuei459 – 4591N6-acetyllysineBy similarity
    Modified residuei490 – 4901N6-acetyllysineBy similarity
    Modified residuei493 – 4931PhosphotyrosineBy similarity
    Modified residuei586 – 5861N6-acetyllysineBy similarity
    Modified residuei599 – 5991S-nitrosocysteineBy similarity

    Post-translational modificationi

    S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1.By similarity
    ISGylated.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein, S-nitrosylation, Ubl conjugation

    Proteomic databases

    PRIDEiP46633.

    Interactioni

    Subunit structurei

    Homodimer. Interacts with AHSA1, FNIP1, HSF1, SMYD3 and TOM34. Interacts with TERT; the interaction, together with PTGES3, is required for correct assembly and stabilization of the TERT holoenzyme complex. Interacts with CHORDC1 and DNAJC7. Interacts with STUB1 and UBE2N; may couple the chaperone and ubiquitination systems By similarity. Following LPS binding, may form a complex with CXCR4, GDF5 and HSPA8 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP46633.
    SMRiP46633. Positions 9-223, 294-697.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni683 – 73351Required for homodimerizationBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi729 – 7335TPR repeat-binding

    Domaini

    The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins like the co-chaperone STUB1.By similarity

    Sequence similaritiesi

    Belongs to the heat shock protein 90 family.Curated

    Phylogenomic databases

    HOVERGENiHBG007374.
    KOiK04079.

    Family and domain databases

    Gene3Di3.30.565.10. 2 hits.
    HAMAPiMF_00505. HSP90.
    InterProiIPR003594. HATPase_ATP-bd.
    IPR019805. Heat_shock_protein_90_CS.
    IPR001404. Hsp90_fam.
    IPR020575. Hsp90_N.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    [Graphical view]
    PANTHERiPTHR11528. PTHR11528. 1 hit.
    PfamiPF02518. HATPase_c. 1 hit.
    PF00183. HSP90. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002583. Hsp90. 1 hit.
    PRINTSiPR00775. HEATSHOCK90.
    SMARTiSM00387. HATPase_c. 1 hit.
    [Graphical view]
    SUPFAMiSSF54211. SSF54211. 1 hit.
    SSF55874. SSF55874. 1 hit.
    PROSITEiPS00298. HSP90. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P46633-1 [UniParc]FASTAAdd to Basket

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    MPEETQTQDQ PMEEEEVETF AFQAEIAQLM SLIINTFYSN KEIFLRELIS    50
    NSSDALDKIR YESLTDPSKL DSGKELHINI IPNKQDRTLT IVDTGIGMTK 100
    ADLINNLGTI AKSGTKAFME ALQAGADISM IGQFGVGFYT AYLVAEKVTV 150
    ITKHNDDEQY AWESSAGGSF TVRTDTGEPM GRGTKVILHL KEDQTEYMEE 200
    RRIKEIVKKH SQFIGYPITL FVEKERDKEV SDDEAEEKED KEEEKEKEEK 250
    GIDDKPEIED VGSDEEEEEK KDGDKKKKKK IKEKYIDQEE LNKTKPIWTR 300
    NPDDITNEEY GEFYKSLTND WEEHLAVKHF SVEGQLEFRA LLFVPRRAPF 350
    DLFENRKKKN NIKLYVRRVF IMDNCEELFP EYLNFIRGVV DSEDLPLNIS 400
    REILQQSKIL KVIRKNLVRK CLELFHELAE DKENYKKFYE QFSKNIKLGI 450
    HEDSQNRKKL SELLRYYTSA SGDEMVSLKD YCTRMKENQK HIYFITGETK 500
    DQVANSAFVE RLRKHGLEVI YMIEPIDEYC VQQLKEFEGK TLVSVTKEGL 550
    ELPEDEEEKK KQEEKKTKFE NLCKIMKDIL EKKVEKVVVS NRLVTSPCCI 600
    VTSTYGWTAN MERIIKAQAL RDNSTMGYMA AKKHLEINPD HSIIETLRQK 650
    AEADKNDKSV KDLVILLYET ALLSSGFSLE DPQTHANRIY RMIKLGLGID 700
    EDDPTVDDTS AAVTEEMPPL EGDDDTSRME EVD 733
    Length:733
    Mass (Da):84,849
    Last modified:January 23, 2007 - v2
    Checksum:i95EECAFD0E410057
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L33676 mRNA. Translation: AAA36992.1.
    RefSeqiNP_001233750.1. NM_001246821.1.

    Genome annotation databases

    GeneIDi100689397.
    KEGGicge:100689397.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L33676 mRNA. Translation: AAA36992.1 .
    RefSeqi NP_001233750.1. NM_001246821.1.

    3D structure databases

    ProteinModelPortali P46633.
    SMRi P46633. Positions 9-223, 294-697.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P46633.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100689397.
    KEGGi cge:100689397.

    Organism-specific databases

    CTDi 3320.

    Phylogenomic databases

    HOVERGENi HBG007374.
    KOi K04079.

    Family and domain databases

    Gene3Di 3.30.565.10. 2 hits.
    HAMAPi MF_00505. HSP90.
    InterProi IPR003594. HATPase_ATP-bd.
    IPR019805. Heat_shock_protein_90_CS.
    IPR001404. Hsp90_fam.
    IPR020575. Hsp90_N.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    [Graphical view ]
    PANTHERi PTHR11528. PTHR11528. 1 hit.
    Pfami PF02518. HATPase_c. 1 hit.
    PF00183. HSP90. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002583. Hsp90. 1 hit.
    PRINTSi PR00775. HEATSHOCK90.
    SMARTi SM00387. HATPase_c. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54211. SSF54211. 1 hit.
    SSF55874. SSF55874. 1 hit.
    PROSITEi PS00298. HSP90. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of a cDNA clone encoding HSP90A from Chinese hamster cells."
      Chen M.-S.M.C., Laszlo A.
      Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiHS90A_CRIGR
    AccessioniPrimary (citable) accession number: P46633
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 96 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3