P46616 (AA2AR_CAVPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenosine receptor A2a

Gene names

Name:

ADORA2A

Organism

Cavia porcellus (Guinea pig) [Reference proteome]

Taxonomic identifier

10141 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Subunit structure	Interacts (via cytoplasmic C-terminal domain) with USP4; the interaction is direct By similarity. May interact with DRD4 By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Domain	The cytoplasmic C-terminal domain is necessary for targeting the non-ubiquitinated form of this protein to the cell surface By similarity.
Post-translational modification	Ubiquitinated. Deubiquitinated by USP4; leading to stabilization and expression at the cell surface By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adenylate cyclase-activating G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: Compara eating behavior Inferred from electronic annotation. Source: Compara locomotory behavior Inferred from electronic annotation. Source: Compara negative regulation of alpha-beta T cell activation Inferred from electronic annotation. Source: Compara negative regulation of inflammatory response Inferred from electronic annotation. Source: Compara positive regulation of apoptotic signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of cAMP biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of synaptic transmission, GABAergic Inferred from electronic annotation. Source: Compara regulation of inhibitory postsynaptic membrane potential Inferred from electronic annotation. Source: Compara response to amphetamine Inferred from electronic annotation. Source: Compara synaptic transmission, dopaminergic Inferred from electronic annotation. Source: Compara
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW intracellular organelle part Inferred from electronic annotation. Source: Compara plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	G-protein coupled adenosine receptor activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 409	409	Adenosine receptor A2a		PRO_0000068997
Regions
	Topological domain	1 – 4	4	Extracellular By similarity
	Transmembrane	5 – 29	25	Helical; Name=1; By similarity
	Topological domain	30 – 39	10	Cytoplasmic By similarity
	Transmembrane	40 – 63	24	Helical; Name=2; By similarity
	Topological domain	64 – 74	11	Extracellular By similarity
	Transmembrane	75 – 97	23	Helical; Name=3; By similarity
	Topological domain	98 – 117	20	Cytoplasmic By similarity
	Transmembrane	118 – 140	23	Helical; Name=4; By similarity
	Topological domain	141 – 170	30	Extracellular By similarity
	Transmembrane	171 – 195	25	Helical; Name=5; By similarity
	Topological domain	196 – 231	36	Cytoplasmic By similarity
	Transmembrane	232 – 255	24	Helical; Name=6; By similarity
	Topological domain	256 – 263	8	Extracellular By similarity
	Transmembrane	264 – 287	24	Helical; Name=7; By similarity
	Topological domain	288 – 409	122	Cytoplasmic By similarity
	Region	165 – 174	10	Agonist binding By similarity
	Region	243 – 250	8	Agonist binding By similarity
	Region	261 – 271	11	Agonist binding By similarity
Amino acid modifications
	Glycosylation	142	1	N-linked (GlcNAc...) Potential
	Glycosylation	151	1	N-linked (GlcNAc...) Potential
	Disulfide bond	68 ↔ 156		By similarity
	Disulfide bond	71 ↔ 143		By similarity
	Disulfide bond	74 ↔ 163		By similarity
	Disulfide bond	256 ↔ 259		By similarity
Experimental info
	Sequence conflict	94	1	T → A Ref.2
	Sequence conflict	96	1	T → A Ref.2
	Sequence conflict	228	1	P → A in AAA18421. Ref.2
	Sequence conflict	243	1	C → W in AAA18421. Ref.2
	Sequence conflict	247	1	N → H in AAA18421. Ref.2
	Sequence conflict	260	1	D → G in AAA18421. Ref.2
	Sequence conflict	268 – 269	2	YL → SM in AAA18421. Ref.2
	Sequence conflict	349	1	L → P in AAA18421. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P46616 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: C4263E63B9000633	FASTA	409	44,943
	10 20 30 40 50 60 MSSSVYITVE LVIAVLAILG NVLVCWAVWI NSNLQNVTNY FVVSLAAADI AVGVLAIPFA 70 80 90 100 110 120 ITISTGFCAA CHGCLFFACF VLVLTQSSIF SLLTITIDRY IAIRIPLRYN GLVTCTRAKG 130 140 150 160 170 180 IIAICWVLSF AIGLTPMLGW NNCSQPKGDK NHSESCDEGQ VTCLFEDVVP MNYMVYYNFF 190 200 210 220 230 240 AFVLVPLLLM LGIYLRIFLA ARRQLKQMES QPLPGERTRS TLQKEVHPAK SLAIIVGLFA 250 260 270 280 290 300 LCCLPLNIIN CFTFFCPECD HAPPWLMYLT IILSHGNSVV NPLIYAYRIR EFRQTFRKII 310 320 330 340 350 360 RSHILRRREL FKAGGTSARA SAAHSPEGEQ VSLRLNGHPP GVWANGSALR PEQRPNGYVL 370 380 390 400 GLVSGRSAQR SHGDASLSDV ELLSHEHKGT CPESPSLEDP PAHGGAGVS

References

[1]	"Functional coupling of adenosine A2a receptor to inhibition of the mitogen-activated protein kinase cascade in Chinese hamster ovary cells." Hirano D., Ogasawara H., Kodama H., Waga I., Sakanaka C., Shimizu T., Nakamura M. Biochem. J. 316:81-86(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Hartley. Tissue: Leukocyte.
[2]	"Cloning and expression of the A2a adenosine receptor from guinea pig brain." Meng F., Xie G.X., Chalmers D., Morgan C., Watson S.J., Akil H. Neurochem. Res. 19:613-621(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Hartley. Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D63674 mRNA. Translation: BAA09825.1. U04201 mRNA. Translation: AAA18421.1.
PIR	I48095. S68247.
RefSeq	NP_001166204.1. NM_001172733.1.
3D structure databases
ProteinModelPortal	P46616.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100135473.
Organism-specific databases
CTD	135.
Phylogenomic databases
eggNOG	NOG287824.
HOGENOM	HOG000015770.
HOVERGEN	HBG106962.
InParanoid	P46616.
OrthoDB	EOG44XJHH.
Family and domain databases
InterPro	IPR001513. Adeno_A2A_rcpt. IPR001634. Adenosn_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00553. ADENOSINA2AR. PR00424. ADENOSINER. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P46616.
ChEMBL	CHEMBL2605.

Entry information

Entry name

AA2AR_CAVPO

Accession

Primary (citable) accession number: P46616

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program