Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pyruvate kinase

Gene

CDC19

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Has antigenic properties.

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • K+By similarity

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (TDH3)
  2. Phosphoglycerate kinase (PGK1)
  3. Phosphoglycerate mutase (GPM2), Phosphoglycerate mutase (GPM1)
  4. Enolase 1 (ENO1)
  5. Pyruvate kinase (CDC19)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei53SubstrateBy similarity1
Metal bindingi55PotassiumBy similarity1
Metal bindingi57PotassiumBy similarity1
Metal bindingi88PotassiumBy similarity1
Metal bindingi89Potassium; via carbonyl oxygenBy similarity1
Sitei244Transition state stabilizerBy similarity1
Metal bindingi246MagnesiumSequence analysis1
Binding sitei269Substrate; via amide nitrogenBy similarity1
Metal bindingi270MagnesiumBy similarity1
Binding sitei270Substrate; via amide nitrogenBy similarity1
Binding sitei302SubstrateBy similarity1
Binding sitei341ADPSequence analysis1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: UniProtKB-KW
  • magnesium ion binding Source: InterPro
  • potassium ion binding Source: InterPro
  • pyruvate kinase activity Source: CGD

GO - Biological processi

  • cellular response to starvation Source: CGD
  • filamentous growth Source: CGD
  • filamentous growth of a population of unicellular organisms in response to biotic stimulus Source: CGD
  • filamentous growth of a population of unicellular organisms in response to starvation Source: CGD
  • glycolytic process Source: CGD
  • induction by symbiont of host defense response Source: CGD

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:CDC19
Synonyms:PYK1
Ordered Locus Names:CAALFM_C205460WA
ORF Names:CaO19.11059, CaO19.3575
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
Proteomesi
  • UP000000559 Componenti: Chromosome 2

Organism-specific databases

CGDiCAL0000192883 CDC19

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001121091 – 504Pyruvate kinaseAdd BLAST504

Proteomic databases

PRIDEiP46614

2D gel databases

COMPLUYEAST-2DPAGEiP46614

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP46614
SMRiP46614
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

InParanoidiP46614
KOiK00873
OMAiKHEAIEQ
OrthoDBiEOG092C1RXZ

Family and domain databases

CDDicd00288 Pyruvate_Kinase, 1 hit
Gene3Di2.40.33.10, 1 hit
3.40.1380.20, 2 hits
InterProiView protein in InterPro
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR018209 Pyrv_Knase_AS
IPR015793 Pyrv_Knase_brl
IPR015795 Pyrv_Knase_C
IPR036918 Pyrv_Knase_C_sf
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00224 PK, 1 hit
PF02887 PK_C, 1 hit
PRINTSiPR01050 PYRUVTKNASE
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 2 hits
SSF52935 SSF52935, 1 hit
TIGRFAMsiTIGR01064 pyruv_kin, 1 hit
PROSITEiView protein in PROSITE
PS00110 PYRUVATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

P46614-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHSSLSWLS NFNVETVPSK YLRRSSIIGT IGPKTNNVDV LVKLRKAGLN
60 70 80 90 100
VVRMNFSHGS YEYHQSVIDN ARKSEEVYKG RPLAIALDTK GPEIRTGTTI
110 120 130 140 150
GDKDYPIPPN HEMIFTTDDA YKTKCDDKVM YIDYKNITKV IAPGKIIYVD
160 170 180 190 200
DGVLSFEVIS VDDEQTLKVR SLNAGKISSH KGVNLPGTDV DLPALSEKDI
210 220 230 240 250
ADIKFGVKNK VHMIFASFIR TANDVLEIRK VLGEEGKDIQ IISKIENQQG
260 270 280 290 300
VNNFDEILEV TDGVMVARGD LGIEIPAPQV FVVQKQLIAK CNLAAKPVIC
310 320 330 340 350
ATQMLESMTY NPRPTRAEVS DVGNAILDGA DCVMLSGETA KGNYPVEAVS
360 370 380 390 400
MMHNTCLTAE KAIAYPQLFN ELRSLAKKPT ATTETCAVAA VSAAYEQDAK
410 420 430 440 450
AIVVLSTSGL SARLVSKYKP DVPILMVTRN ERAAKFSHLY RGVYPFIYDK
460 470 480 490 500
PSIENWQEDV ENRLRWAVSE AVELGIISKG DSIVTVQGWT RGSGHSNTVR

IVQA
Length:504
Mass (Da):55,445
Last modified:October 19, 2011 - v3
Checksum:i38518DDA54735113
GO

Sequence cautioni

The sequence S65775 differs from that shown. Reason: Frameshift at several positions.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti94I → T in S65775 (PubMed:8406815).Curated1
Sequence conflicti98T → H in S65775 (PubMed:8406815).Curated1
Sequence conflicti106P → T in S65775 (PubMed:8406815).Curated1
Sequence conflicti131Y → I in S65775 (PubMed:8406815).Curated1
Sequence conflicti164E → Q in S65775 (PubMed:8406815).Curated1
Sequence conflicti176K → M in S65775 (PubMed:8406815).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP017624 Genomic DNA Translation: AOW27559.1
S65775 mRNA No translation available.
RefSeqiXP_714934.1, XM_709841.1

Genome annotation databases

EnsemblFungiiAOW27559; AOW27559; CAALFM_C205460WA
GeneIDi3643438
KEGGical:CAALFM_C205460WA

Similar proteinsi

Entry informationi

Entry nameiKPYK_CANAL
AccessioniPrimary (citable) accession number: P46614
Secondary accession number(s): A0A1D8PHF3, Q59ZE4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 19, 2011
Last modified: March 28, 2018
This is version 112 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health