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P46614

- KPYK_CANAL

UniProt

P46614 - KPYK_CANAL

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Protein

Pyruvate kinase

Gene

CDC19

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Magnesium.By similarity
Potassium.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei53 – 531SubstrateBy similarity
Metal bindingi55 – 551PotassiumBy similarity
Metal bindingi57 – 571PotassiumBy similarity
Metal bindingi88 – 881PotassiumBy similarity
Metal bindingi89 – 891Potassium; via carbonyl oxygenBy similarity
Sitei244 – 2441Transition state stabilizerBy similarity
Metal bindingi246 – 2461MagnesiumSequence Analysis
Binding sitei269 – 2691Substrate; via amide nitrogenBy similarity
Metal bindingi270 – 2701MagnesiumBy similarity
Binding sitei270 – 2701Substrate; via amide nitrogenBy similarity
Binding sitei302 – 3021SubstrateBy similarity
Binding sitei341 – 3411ADPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: CGD

GO - Biological processi

  1. cellular response to starvation Source: CGD
  2. filamentous growth Source: CGD
  3. filamentous growth of a population of unicellular organisms in response to biotic stimulus Source: CGD
  4. filamentous growth of a population of unicellular organisms in response to starvation Source: CGD
  5. glycolytic process Source: CGD
  6. induction by symbiont of host defense response Source: CGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:CDC19
Synonyms:PYK1
ORF Names:CaO19.11059, CaO19.3575
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
ProteomesiUP000000559: Unassembled WGS sequence

Organism-specific databases

CGDiCAL0005977. CDC19.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cell surface Source: CGD
  2. cytoplasm Source: UniProtKB-KW
  3. hyphal cell wall Source: CGD
  4. plasma membrane Source: CGD
  5. yeast-form cell wall Source: CGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 504504Pyruvate kinasePRO_0000112109Add
BLAST

2D gel databases

COMPLUYEAST-2DPAGEP46614.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi5476.CAL0005977.

Structurei

3D structure databases

ProteinModelPortaliP46614.
SMRiP46614. Positions 22-501.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiCOG0469.
InParanoidiP46614.
KOiK00873.
OrthoDBiEOG7M6DJC.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46614-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSHSSLSWLS NFNVETVPSK YLRRSSIIGT IGPKTNNVDV LVKLRKAGLN
60 70 80 90 100
VVRMNFSHGS YEYHQSVIDN ARKSEEVYKG RPLAIALDTK GPEIRTGTTI
110 120 130 140 150
GDKDYPIPPN HEMIFTTDDA YKTKCDDKVM YIDYKNITKV IAPGKIIYVD
160 170 180 190 200
DGVLSFEVIS VDDEQTLKVR SLNAGKISSH KGVNLPGTDV DLPALSEKDI
210 220 230 240 250
ADIKFGVKNK VHMIFASFIR TANDVLEIRK VLGEEGKDIQ IISKIENQQG
260 270 280 290 300
VNNFDEILEV TDGVMVARGD LGIEIPAPQV FVVQKQLIAK CNLAAKPVIC
310 320 330 340 350
ATQMLESMTY NPRPTRAEVS DVGNAILDGA DCVMLSGETA KGNYPVEAVS
360 370 380 390 400
MMHNTCLTAE KAIAYPQLFN ELRSLAKKPT ATTETCAVAA VSAAYEQDAK
410 420 430 440 450
AIVVLSTSGL SARLVSKYKP DVPILMVTRN ERAAKFSHLY RGVYPFIYDK
460 470 480 490 500
PSIENWQEDV ENRLRWAVSE AVELGIISKG DSIVTVQGWT RGSGHSNTVR

IVQA
Length:504
Mass (Da):55,445
Last modified:October 19, 2011 - v3
Checksum:i38518DDA54735113
GO

Sequence cautioni

The sequence S65775 differs from that shown. Reason: Frameshift at several positions.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti94 – 941I → T in S65775. (PubMed:8406815)Curated
Sequence conflicti98 – 981T → H in S65775. (PubMed:8406815)Curated
Sequence conflicti106 – 1061P → T in S65775. (PubMed:8406815)Curated
Sequence conflicti131 – 1311Y → I in S65775. (PubMed:8406815)Curated
Sequence conflicti164 – 1641E → Q in S65775. (PubMed:8406815)Curated
Sequence conflicti176 – 1761K → M in S65775. (PubMed:8406815)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AACQ01000094 Genomic DNA. Translation: EAK95894.1.
AACQ01000093 Genomic DNA. Translation: EAK95958.1.
S65775 mRNA. No translation available.
RefSeqiXP_714934.1. XM_709841.1.
XP_714997.1. XM_709904.1.

Genome annotation databases

GeneIDi3643409.
3643438.
KEGGical:CaO19.11059.
cal:CaO19.3575.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AACQ01000094 Genomic DNA. Translation: EAK95894.1 .
AACQ01000093 Genomic DNA. Translation: EAK95958.1 .
S65775 mRNA. No translation available.
RefSeqi XP_714934.1. XM_709841.1.
XP_714997.1. XM_709904.1.

3D structure databases

ProteinModelPortali P46614.
SMRi P46614. Positions 22-501.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5476.CAL0005977.

2D gel databases

COMPLUYEAST-2DPAGE P46614.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3643409.
3643438.
KEGGi cal:CaO19.11059.
cal:CaO19.3575.

Organism-specific databases

CGDi CAL0005977. CDC19.

Phylogenomic databases

eggNOGi COG0469.
InParanoidi P46614.
KOi K00873.
OrthoDBi EOG7M6DJC.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SC5314 / ATCC MYA-2876.
  2. "Glycolytic enzymes of Candida albicans are nonubiquitous immunogens during candidiasis."
    Swoboda R.K., Bertram G., Hollander H., Greenspan D., Greenspan J.S., Gow N.A., Gooday G.W., Brown A.J.
    Infect. Immun. 61:4263-4271(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-181.
  3. "Cross-species identification of novel Candida albicans immunogenic proteins by combination of two-dimensional polyacrylamide gel electrophoresis and mass spectrometry."
    Pardo M., Ward M., Pitarch A., Sanchez M., Nombela C., Blackstock W., Gil C.
    Electrophoresis 21:2651-2659(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 221-229 AND 404-409, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: SC5314 / ATCC MYA-2876.
  4. "Proteomics-based identification of novel Candida albicans antigens for diagnosis of systemic candidiasis in patients with underlying hematological malignancies."
    Pitarch A., Abian J., Carrascal M., Sanchez M., Nombela C., Gil C.
    Proteomics 4:3084-3106(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 404-409, SUBCELLULAR LOCATION, ANTIGENICITY.
    Strain: SC5314 / ATCC MYA-2876.
    Tissue: Protoplast.

Entry informationi

Entry nameiKPYK_CANAL
AccessioniPrimary (citable) accession number: P46614
Secondary accession number(s): Q59ZE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 19, 2011
Last modified: October 29, 2014
This is version 93 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Has antigenic properties.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3