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P46614

- KPYK_CANAL

UniProt

P46614 - KPYK_CANAL

Protein

Pyruvate kinase

Gene

CDC19

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 3 (19 Oct 2011)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.By similarity
    Potassium.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei53 – 531SubstrateBy similarity
    Metal bindingi55 – 551PotassiumBy similarity
    Metal bindingi57 – 571PotassiumBy similarity
    Metal bindingi88 – 881PotassiumBy similarity
    Metal bindingi89 – 891Potassium; via carbonyl oxygenBy similarity
    Sitei244 – 2441Transition state stabilizerBy similarity
    Metal bindingi246 – 2461MagnesiumSequence Analysis
    Binding sitei269 – 2691Substrate; via amide nitrogenBy similarity
    Metal bindingi270 – 2701MagnesiumBy similarity
    Binding sitei270 – 2701Substrate; via amide nitrogenBy similarity
    Binding sitei302 – 3021SubstrateBy similarity
    Binding sitei341 – 3411ADPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: CGD

    GO - Biological processi

    1. cellular response to starvation Source: CGD
    2. filamentous growth Source: CGD
    3. filamentous growth of a population of unicellular organisms in response to biotic stimulus Source: CGD
    4. filamentous growth of a population of unicellular organisms in response to starvation Source: CGD
    5. glycolytic process Source: CGD
    6. induction by symbiont of host defense response Source: CGD

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Pyruvate

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase (EC:2.7.1.40)
    Short name:
    PK
    Gene namesi
    Name:CDC19
    Synonyms:PYK1
    ORF Names:CaO19.11059, CaO19.3575
    OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
    Taxonomic identifieri237561 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida
    ProteomesiUP000000559: Unassembled WGS sequence

    Organism-specific databases

    CGDiCAL0005977. CDC19.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cell surface Source: CGD
    2. cytoplasm Source: UniProtKB-SubCell
    3. hyphal cell wall Source: CGD
    4. plasma membrane Source: CGD
    5. yeast-form cell wall Source: CGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 504504Pyruvate kinasePRO_0000112109Add
    BLAST

    2D gel databases

    COMPLUYEAST-2DPAGEP46614.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi5476.CAL0005977.

    Structurei

    3D structure databases

    ProteinModelPortaliP46614.
    SMRiP46614. Positions 22-501.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0469.
    KOiK00873.
    OrthoDBiEOG7M6DJC.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P46614-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSHSSLSWLS NFNVETVPSK YLRRSSIIGT IGPKTNNVDV LVKLRKAGLN    50
    VVRMNFSHGS YEYHQSVIDN ARKSEEVYKG RPLAIALDTK GPEIRTGTTI 100
    GDKDYPIPPN HEMIFTTDDA YKTKCDDKVM YIDYKNITKV IAPGKIIYVD 150
    DGVLSFEVIS VDDEQTLKVR SLNAGKISSH KGVNLPGTDV DLPALSEKDI 200
    ADIKFGVKNK VHMIFASFIR TANDVLEIRK VLGEEGKDIQ IISKIENQQG 250
    VNNFDEILEV TDGVMVARGD LGIEIPAPQV FVVQKQLIAK CNLAAKPVIC 300
    ATQMLESMTY NPRPTRAEVS DVGNAILDGA DCVMLSGETA KGNYPVEAVS 350
    MMHNTCLTAE KAIAYPQLFN ELRSLAKKPT ATTETCAVAA VSAAYEQDAK 400
    AIVVLSTSGL SARLVSKYKP DVPILMVTRN ERAAKFSHLY RGVYPFIYDK 450
    PSIENWQEDV ENRLRWAVSE AVELGIISKG DSIVTVQGWT RGSGHSNTVR 500
    IVQA 504
    Length:504
    Mass (Da):55,445
    Last modified:October 19, 2011 - v3
    Checksum:i38518DDA54735113
    GO

    Sequence cautioni

    The sequence S65775 differs from that shown. Reason: Frameshift at several positions.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti94 – 941I → T in S65775. (PubMed:8406815)Curated
    Sequence conflicti98 – 981T → H in S65775. (PubMed:8406815)Curated
    Sequence conflicti106 – 1061P → T in S65775. (PubMed:8406815)Curated
    Sequence conflicti131 – 1311Y → I in S65775. (PubMed:8406815)Curated
    Sequence conflicti164 – 1641E → Q in S65775. (PubMed:8406815)Curated
    Sequence conflicti176 – 1761K → M in S65775. (PubMed:8406815)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AACQ01000094 Genomic DNA. Translation: EAK95894.1.
    AACQ01000093 Genomic DNA. Translation: EAK95958.1.
    S65775 mRNA. No translation available.
    RefSeqiXP_714934.1. XM_709841.1.
    XP_714997.1. XM_709904.1.

    Genome annotation databases

    GeneIDi3643409.
    3643438.
    KEGGical:CaO19.11059.
    cal:CaO19.3575.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AACQ01000094 Genomic DNA. Translation: EAK95894.1 .
    AACQ01000093 Genomic DNA. Translation: EAK95958.1 .
    S65775 mRNA. No translation available.
    RefSeqi XP_714934.1. XM_709841.1.
    XP_714997.1. XM_709904.1.

    3D structure databases

    ProteinModelPortali P46614.
    SMRi P46614. Positions 22-501.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5476.CAL0005977.

    2D gel databases

    COMPLUYEAST-2DPAGE P46614.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3643409.
    3643438.
    KEGGi cal:CaO19.11059.
    cal:CaO19.3575.

    Organism-specific databases

    CGDi CAL0005977. CDC19.

    Phylogenomic databases

    eggNOGi COG0469.
    KOi K00873.
    OrthoDBi EOG7M6DJC.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SC5314 / ATCC MYA-2876.
    2. "Glycolytic enzymes of Candida albicans are nonubiquitous immunogens during candidiasis."
      Swoboda R.K., Bertram G., Hollander H., Greenspan D., Greenspan J.S., Gow N.A., Gooday G.W., Brown A.J.
      Infect. Immun. 61:4263-4271(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-181.
    3. "Cross-species identification of novel Candida albicans immunogenic proteins by combination of two-dimensional polyacrylamide gel electrophoresis and mass spectrometry."
      Pardo M., Ward M., Pitarch A., Sanchez M., Nombela C., Blackstock W., Gil C.
      Electrophoresis 21:2651-2659(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 221-229 AND 404-409, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: SC5314 / ATCC MYA-2876.
    4. "Proteomics-based identification of novel Candida albicans antigens for diagnosis of systemic candidiasis in patients with underlying hematological malignancies."
      Pitarch A., Abian J., Carrascal M., Sanchez M., Nombela C., Gil C.
      Proteomics 4:3084-3106(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 404-409, SUBCELLULAR LOCATION, ANTIGENICITY.
      Strain: SC5314 / ATCC MYA-2876.
      Tissue: Protoplast.

    Entry informationi

    Entry nameiKPYK_CANAL
    AccessioniPrimary (citable) accession number: P46614
    Secondary accession number(s): Q59ZE4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: October 19, 2011
    Last modified: October 1, 2014
    This is version 92 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Has antigenic properties.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Candida albicans
      Candida albicans: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3