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P46614 (KPYK_CANAL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate kinase

Short name=PK
EC=2.7.1.40
Gene names
Name:CDC19
Synonyms:PYK1
ORF Names:CaO19.11059, CaO19.3575
OrganismCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]
Taxonomic identifier237561 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida

Protein attributes

Sequence length504 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium By similarity.

Potassium By similarity.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer By similarity.

Subcellular location

Cytoplasm Ref.4.

Miscellaneous

Has antigenic properties.

Sequence similarities

Belongs to the pyruvate kinase family.

Sequence caution

The sequence S65775 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
Pyruvate
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to starvation

Inferred from mutant phenotype PubMed 12773383. Source: CGD

filamentous growth

Inferred from mutant phenotype PubMed 12773383. Source: CGD

filamentous growth of a population of unicellular organisms in response to biotic stimulus

Inferred from mutant phenotype PubMed 12773383. Source: CGD

filamentous growth of a population of unicellular organisms in response to starvation

Inferred from mutant phenotype PubMed 12773383. Source: CGD

glycolysis

Inferred from sequence or structural similarity Ref.2. Source: CGD

induction by symbiont of host defense response

Inferred from direct assay PubMed 11681208. Source: CGD

   Cellular_componentcell surface

Inferred from direct assay PubMed 12782322. Source: CGD

cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

hyphal cell wall

Inferred from direct assay PubMed 1383159. Source: CGD

plasma membrane

Inferred from direct assay PubMed 19824013. Source: CGD

yeast-form cell wall

Inferred from direct assay PubMed 12782322. Source: CGD

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

potassium ion binding

Inferred from electronic annotation. Source: InterPro

pyruvate kinase activity

Inferred from sequence or structural similarity Ref.2. Source: CGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 504504Pyruvate kinase
PRO_0000112109

Sites

Metal binding551Potassium By similarity
Metal binding571Potassium By similarity
Metal binding881Potassium By similarity
Metal binding891Potassium; via carbonyl oxygen By similarity
Metal binding2461Magnesium Potential
Metal binding2701Magnesium By similarity
Binding site531Substrate By similarity
Binding site2691Substrate; via amide nitrogen By similarity
Binding site2701Substrate; via amide nitrogen By similarity
Binding site3021Substrate By similarity
Binding site3411ADP Potential
Site2441Transition state stabilizer By similarity

Experimental info

Sequence conflict941I → T in S65775. Ref.2
Sequence conflict981T → H in S65775. Ref.2
Sequence conflict1061P → T in S65775. Ref.2
Sequence conflict1311Y → I in S65775. Ref.2
Sequence conflict1641E → Q in S65775. Ref.2
Sequence conflict1761K → M in S65775. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P46614 [UniParc].

Last modified October 19, 2011. Version 3.
Checksum: 38518DDA54735113

FASTA50455,445
        10         20         30         40         50         60 
MSHSSLSWLS NFNVETVPSK YLRRSSIIGT IGPKTNNVDV LVKLRKAGLN VVRMNFSHGS 

        70         80         90        100        110        120 
YEYHQSVIDN ARKSEEVYKG RPLAIALDTK GPEIRTGTTI GDKDYPIPPN HEMIFTTDDA 

       130        140        150        160        170        180 
YKTKCDDKVM YIDYKNITKV IAPGKIIYVD DGVLSFEVIS VDDEQTLKVR SLNAGKISSH 

       190        200        210        220        230        240 
KGVNLPGTDV DLPALSEKDI ADIKFGVKNK VHMIFASFIR TANDVLEIRK VLGEEGKDIQ 

       250        260        270        280        290        300 
IISKIENQQG VNNFDEILEV TDGVMVARGD LGIEIPAPQV FVVQKQLIAK CNLAAKPVIC 

       310        320        330        340        350        360 
ATQMLESMTY NPRPTRAEVS DVGNAILDGA DCVMLSGETA KGNYPVEAVS MMHNTCLTAE 

       370        380        390        400        410        420 
KAIAYPQLFN ELRSLAKKPT ATTETCAVAA VSAAYEQDAK AIVVLSTSGL SARLVSKYKP 

       430        440        450        460        470        480 
DVPILMVTRN ERAAKFSHLY RGVYPFIYDK PSIENWQEDV ENRLRWAVSE AVELGIISKG 

       490        500 
DSIVTVQGWT RGSGHSNTVR IVQA 

« Hide

References

« Hide 'large scale' references
[1]"The diploid genome sequence of Candida albicans."
Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S.
Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SC5314 / ATCC MYA-2876.
[2]"Glycolytic enzymes of Candida albicans are nonubiquitous immunogens during candidiasis."
Swoboda R.K., Bertram G., Hollander H., Greenspan D., Greenspan J.S., Gow N.A., Gooday G.W., Brown A.J.
Infect. Immun. 61:4263-4271(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-181.
[3]"Cross-species identification of novel Candida albicans immunogenic proteins by combination of two-dimensional polyacrylamide gel electrophoresis and mass spectrometry."
Pardo M., Ward M., Pitarch A., Sanchez M., Nombela C., Blackstock W., Gil C.
Electrophoresis 21:2651-2659(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 221-229 AND 404-409, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: SC5314 / ATCC MYA-2876.
[4]"Proteomics-based identification of novel Candida albicans antigens for diagnosis of systemic candidiasis in patients with underlying hematological malignancies."
Pitarch A., Abian J., Carrascal M., Sanchez M., Nombela C., Gil C.
Proteomics 4:3084-3106(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 404-409, SUBCELLULAR LOCATION, ANTIGENICITY.
Strain: SC5314 / ATCC MYA-2876.
Tissue: Protoplast.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AACQ01000094 Genomic DNA. Translation: EAK95894.1.
AACQ01000093 Genomic DNA. Translation: EAK95958.1.
S65775 mRNA. No translation available.
RefSeqXP_714934.1. XM_709841.1.
XP_714997.1. XM_709904.1.

3D structure databases

ProteinModelPortalP46614.
SMRP46614. Positions 22-501.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5476.CAL0005977.

2D gel databases

COMPLUYEAST-2DPAGEP46614.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3643409.
3643438.
KEGGcal:CaO19.11059.
cal:CaO19.3575.

Organism-specific databases

CGDCAL0005977. CDC19.

Phylogenomic databases

eggNOGCOG0469.
KOK00873.
OrthoDBEOG7M6DJC.

Enzyme and pathway databases

UniPathwayUPA00109; UER00188.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKPYK_CANAL
AccessionPrimary (citable) accession number: P46614
Secondary accession number(s): Q59ZE4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 19, 2011
Last modified: April 16, 2014
This is version 90 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Candida albicans

Candida albicans: entries and gene names