P46614 (KPYK_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyruvate kinase
Short name=PK
EC=2.7.1.40

Gene names

Name:	CDC19
Synonyms:	PYK1
ORF Names:	CaO19.11059, CaO19.3575

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida › Candida albicans

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium By similarity. Potassium By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Ref.4.
Miscellaneous	Has antigenic properties.
Sequence similarities	Belongs to the pyruvate kinase family.
Sequence caution	The sequence S65775 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Pyruvate
Molecular function	Kinase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to biotic stimulus Inferred from mutant phenotype PubMed 12773383. Source: CGD cellular response to starvation Inferred from mutant phenotype PubMed 12773383. Source: CGD filamentous growth of a population of unicellular organisms in response to biotic stimulus Inferred from mutant phenotype PubMed 12773383. Source: CGD filamentous growth of a population of unicellular organisms in response to starvation Inferred from mutant phenotype PubMed 12773383. Source: CGD glycolysis Inferred from sequence or structural similarity Ref.2. Source: CGD induction by symbiont of host defense response Inferred from direct assay PubMed 11681208. Source: CGD
Cellular_component	cell surface Inferred from direct assay PubMed 12782322. Source: CGD cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell hyphal cell wall Inferred from direct assay PubMed 1383159. Source: CGD plasma membrane Inferred from direct assay PubMed 19824013. Source: CGD yeast-form cell wall Inferred from direct assay PubMed 12782322. Source: CGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from sequence or structural similarity Ref.2. Source: CGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 504	504	Pyruvate kinase		PRO_0000112109
Sites
	Metal binding	55	1	Potassium By similarity
	Metal binding	57	1	Potassium By similarity
	Metal binding	88	1	Potassium By similarity
	Metal binding	89	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	246	1	Magnesium Potential
	Metal binding	270	1	Magnesium By similarity
	Binding site	53	1	Substrate By similarity
	Binding site	269	1	Substrate; via amide nitrogen By similarity
	Binding site	270	1	Substrate; via amide nitrogen By similarity
	Binding site	302	1	Substrate By similarity
	Binding site	341	1	ADP Potential
	Site	244	1	Transition state stabilizer By similarity
Experimental info
	Sequence conflict	94	1	I → T in S65775. Ref.2
	Sequence conflict	98	1	T → H in S65775. Ref.2
	Sequence conflict	106	1	P → T in S65775. Ref.2
	Sequence conflict	131	1	Y → I in S65775. Ref.2
	Sequence conflict	164	1	E → Q in S65775. Ref.2
	Sequence conflict	176	1	K → M in S65775. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P46614 [UniParc]. Last modified October 19, 2011. Version 3. Checksum: 38518DDA54735113	FASTA	504	55,445
	10 20 30 40 50 60 MSHSSLSWLS NFNVETVPSK YLRRSSIIGT IGPKTNNVDV LVKLRKAGLN VVRMNFSHGS 70 80 90 100 110 120 YEYHQSVIDN ARKSEEVYKG RPLAIALDTK GPEIRTGTTI GDKDYPIPPN HEMIFTTDDA 130 140 150 160 170 180 YKTKCDDKVM YIDYKNITKV IAPGKIIYVD DGVLSFEVIS VDDEQTLKVR SLNAGKISSH 190 200 210 220 230 240 KGVNLPGTDV DLPALSEKDI ADIKFGVKNK VHMIFASFIR TANDVLEIRK VLGEEGKDIQ 250 260 270 280 290 300 IISKIENQQG VNNFDEILEV TDGVMVARGD LGIEIPAPQV FVVQKQLIAK CNLAAKPVIC 310 320 330 340 350 360 ATQMLESMTY NPRPTRAEVS DVGNAILDGA DCVMLSGETA KGNYPVEAVS MMHNTCLTAE 370 380 390 400 410 420 KAIAYPQLFN ELRSLAKKPT ATTETCAVAA VSAAYEQDAK AIVVLSTSGL SARLVSKYKP 430 440 450 460 470 480 DVPILMVTRN ERAAKFSHLY RGVYPFIYDK PSIENWQEDV ENRLRWAVSE AVELGIISKG 490 500 DSIVTVQGWT RGSGHSNTVR IVQA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.
[2]	"Glycolytic enzymes of Candida albicans are nonubiquitous immunogens during candidiasis." Swoboda R.K., Bertram G., Hollander H., Greenspan D., Greenspan J.S., Gow N.A., Gooday G.W., Brown A.J. Infect. Immun. 61:4263-4271(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-181.
[3]	"Cross-species identification of novel Candida albicans immunogenic proteins by combination of two-dimensional polyacrylamide gel electrophoresis and mass spectrometry." Pardo M., Ward M., Pitarch A., Sanchez M., Nombela C., Blackstock W., Gil C. Electrophoresis 21:2651-2659(2000) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 221-229 AND 404-409, MASS SPECTROMETRY. Strain: SC5314 / ATCC MYA-2876.
[4]	"Proteomics-based identification of novel Candida albicans antigens for diagnosis of systemic candidiasis in patients with underlying hematological malignancies." Pitarch A., Abian J., Carrascal M., Sanchez M., Nombela C., Gil C. Proteomics 4:3084-3106(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 404-409, SUBCELLULAR LOCATION, ANTIGENICITY. Strain: SC5314 / ATCC MYA-2876. Tissue: Protoplast.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACQ01000094 Genomic DNA. Translation: EAK95894.1. AACQ01000093 Genomic DNA. Translation: EAK95958.1. S65775 mRNA. No translation available.
RefSeq	XP_714934.1. XM_709841.1. XP_714997.1. XM_709904.1.
3D structure databases
ProteinModelPortal	P46614.
SMR	P46614. Positions 22-501.
ModBase	Search...
Protein-protein interaction databases
STRING	5476.CAL0005977.
2D gel databases
COMPLUYEAST-2DPAGE	P46614.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3643409. 3643438.
KEGG	cal:CaO19.11059. cal:CaO19.3575.
Organism-specific databases
CGD	CAL0005977. CDC19.
Phylogenomic databases
eggNOG	COG0469.
KO	K00873.
Enzyme and pathway databases
UniPathway	UPA00109; UER00188.
Family and domain databases
Gene3D	2.40.33.10. 1 hit. 3.20.20.60. 2 hits. 3.40.1380.20. 1 hit.
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase. IPR011037. Pyrv_Knase-like_insert_dom. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. IPR015795. Pyrv_Knase_C. IPR015806. Pyrv_Knase_insert_dom. [Graphical view]
PANTHER	PTHR11817. PTHR11817. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
SUPFAM	SSF50800. PK_B_barrel_like. 1 hit. SSF52935. Pyruvate_kinase. 1 hit. SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYK_CANAL

Accession

Primary (citable) accession number: P46614
Secondary accession number(s): Q59ZE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	October 19, 2011
Last modified:	April 3, 2013
This is version 85 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program