Reviewed, UniProtKB/Swiss-Prot P46599 (STE7_CANAL)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase STE7 homolog
    EC=2.7.12.2

Gene names

Name:	STE7
Synonyms:	HST7

Organism

Candida albicans (Yeast)

Taxonomic identifier

5476 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	589 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 589	589	Serine/threonine-protein kinase STE7 homolog		PRO_0000086687
Regions
	Domain	249 – 565	317	Protein kinase
	Nucleotide binding	255 – 263	9	ATP By similarity
Sites
	Active site	374	1	Proton acceptor By similarity
	Binding site	278	1	ATP By similarity
Amino acid modifications
	Modified residue	402	1	Phosphoserine By similarity
	Modified residue	408	1	Phosphothreonine By similarity
Experimental info
	Sequence conflict	83	1	T → Q in AAC49733. Ref.2
	Sequence conflict	241	1	S → A in AAC49733. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P46599-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 53125E4BA5BA870B	FASTA	589	65,207
	10 20 30 40 50 60 MTRTTRIDTQ EATKHKDLPP VPSPLSLSSN PNPECLMESK SLGRKNFKKL SLDASPVKST 70 80 90 100 110 120 SGSLRSSDMM SIKEPTSLRQ KRTRPPPILH LPTASSSATS TPTSNITGSS SASSIQFAQK 130 140 150 160 170 180 SPGSGVIVSQ TLSRPSSAGG IPSSGYSSLN VNQSNRNVDP DNVVSTDMIL NQISNLDLTS 190 200 210 220 230 240 MNHHRQHYQN SHHHLPTTNR KRQTVISSIS PTKSSAASSP LEPQIQSLPA SSQSPIATTS 250 260 270 280 290 300 SLKLNNKDLL TLKQLGSGNS GSVSKILHIP TQKTMAKKII HIDSKSVIQT QIIRELRILH 310 320 330 340 350 360 ECHSPYIIEF YGACLNNNNT IVICMEYCNC GSLDKILPLC ENKQFPTFVL KKLSFAILSG 370 380 390 400 410 420 LTYLYTTHKI IHRDIKPNNV LMTHKGEFKL CDFGVSRELT NSLAMADTFV GTSMYMSPER 430 440 450 460 470 480 IQGLDYGVKS DVWSTGLMLI ELASGVPVWS EDDNNNDDDE DDEDDAYVRQ GSIAAERNGQ 490 500 510 520 530 540 NSPSRSRKNK QKGNGYNSYN GPEGILDLLQ RIVNEDAPTL TNKINPVTKL PYDKYLCQFI 550 560 570 580 DLCLIKDDSV RKTPWQLLED KEHFFKGVEE GVYDKEHKSW AKKIRKCKV

References

[1]	"Constitutive activation of the Saccharomyces cerevisiae mating response pathway by a MAP kinase kinase from Candida albicans." Clark K.L., Feldmann P.J., Dignard D., Larocque R., Brown A.J., Lee M.G., Thomas D.Y., Whiteway M. Mol. Gen. Genet. 249:609-621(1995) [PubMed: 8544826] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: WO-1.
[2]	"A novel MAP-kinase kinase from Candida albicans." Singh P., Ghosh S., Datta A. Gene 190:99-104(1997) [PubMed: 9185854] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 11651 / B792 / 171D.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L19195 Genomic DNA. Translation: AAB59338.1. U72980 Genomic DNA. Translation: AAC49733.1.
PIR	S60154. S60159.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.12.2. 1124.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

STE7_CANAL

Accession

Primary (citable) accession number: P46599
Secondary accession number(s): P78596

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)