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Protein

Ubiquitin-conjugating enzyme E2 4

Gene

ubc4

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Mediates ubiquitination of PEX5.PROSITE-ProRule annotation

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei85Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • positive regulation of mitotic metaphase/anaphase transition Source: PomBase
  • protein polyubiquitination Source: UniProtKB
  • regulation of protein glycosylation Source: PomBase
  • SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: PomBase
  • sterol regulatory element binding protein cleavage Source: PomBase
  • ubiquitin-dependent protein catabolic process Source: PomBase

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-SPO-8866654 E3 ubiquitin ligases ubiquitinate target proteins
R-SPO-9033241 Peroxisomal protein import
R-SPO-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 4 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 4
Ubiquitin carrier protein 4
Ubiquitin-protein ligase 4
Gene namesi
Name:ubc4
ORF Names:SPBC119.02
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC119.02
PomBaseiSPBC119.02 ubc4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825691 – 147Ubiquitin-conjugating enzyme E2 4Add BLAST147

Proteomic databases

MaxQBiP46595
PaxDbiP46595
PRIDEiP46595

PTM databases

iPTMnetiP46595

Interactioni

Protein-protein interaction databases

BioGridi276621, 17 interactors
IntActiP46595, 1 interactor
STRINGi4896.SPBC119.02.1

Structurei

Secondary structure

1147
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 14Combined sources13
Beta strandi20 – 26Combined sources7
Beta strandi29 – 38Combined sources10
Turni44 – 47Combined sources4
Beta strandi49 – 55Combined sources7
Turni58 – 62Combined sources5
Beta strandi66 – 69Combined sources4
Helixi87 – 89Combined sources3
Turni90 – 92Combined sources3
Helixi99 – 111Combined sources13
Helixi121 – 129Combined sources9
Helixi131 – 145Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4II2X-ray2.20C1-147[»]
ProteinModelPortaliP46595
SMRiP46595
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000233455
InParanoidiP46595
KOiK06689
OMAiNDLGRDP
OrthoDBiEOG092C50OL
PhylomeDBiP46595

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

P46595-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALKRINREL ADLGKDPPSS CSAGPVGDDL FHWQATIMGP ADSPYAGGVF
60 70 80 90 100
FLSIHFPTDY PFKPPKVNFT TRIYHPNINS NGSICLDILR DQWSPALTIS
110 120 130 140
KVLLSICSLL TDPNPDDPLV PEIAHVYKTD RSRYELSARE WTRKYAI
Length:147
Mass (Da):16,476
Last modified:November 1, 1995 - v1
Checksum:i3E8BADFF494A91EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L37384 mRNA No translation available.
CU329671 Genomic DNA Translation: CAA17917.1
PIRiT39300
RefSeqiNP_595283.1, NM_001021190.2

Genome annotation databases

EnsemblFungiiSPBC119.02.1; SPBC119.02.1:pep; SPBC119.02
GeneIDi2540083
KEGGispo:SPBC119.02

Similar proteinsi

Entry informationi

Entry nameiUBC4_SCHPO
AccessioniPrimary (citable) accession number: P46595
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 23, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  4. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health