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Protein

Ubiquitin-conjugating enzyme E2 4

Gene

ubc4

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Mediates ubiquitination of PEX5.PROSITE-ProRule annotation

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei85Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • positive regulation of mitotic metaphase/anaphase transition Source: PomBase
  • protein localization to Golgi apparatus Source: PomBase
  • protein polyubiquitination Source: UniProtKB
  • protein processing Source: PomBase
  • protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: PomBase
  • regulation of protein glycosylation Source: PomBase
  • SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: PomBase
  • sterol regulatory element binding protein cleavage Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 4 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 4
Ubiquitin carrier protein 4
Ubiquitin-protein ligase 4
Gene namesi
Name:ubc4
ORF Names:SPBC119.02
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC119.02.
PomBaseiSPBC119.02. ubc4.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • nuclear SCF ubiquitin ligase complex Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825691 – 147Ubiquitin-conjugating enzyme E2 4Add BLAST147

Proteomic databases

MaxQBiP46595.
PRIDEiP46595.

Interactioni

Protein-protein interaction databases

BioGridi276621. 17 interactors.
IntActiP46595. 1 interactor.
MINTiMINT-4690340.

Structurei

Secondary structure

1147
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 14Combined sources13
Beta strandi20 – 26Combined sources7
Beta strandi29 – 38Combined sources10
Turni44 – 47Combined sources4
Beta strandi49 – 55Combined sources7
Turni58 – 62Combined sources5
Beta strandi66 – 69Combined sources4
Helixi87 – 89Combined sources3
Turni90 – 92Combined sources3
Helixi99 – 111Combined sources13
Helixi121 – 129Combined sources9
Helixi131 – 145Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4II2X-ray2.20C1-147[»]
ProteinModelPortaliP46595.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000233455.
InParanoidiP46595.
KOiK06689.
OMAiLYKTDRT.
OrthoDBiEOG092C50OL.
PhylomeDBiP46595.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46595-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALKRINREL ADLGKDPPSS CSAGPVGDDL FHWQATIMGP ADSPYAGGVF
60 70 80 90 100
FLSIHFPTDY PFKPPKVNFT TRIYHPNINS NGSICLDILR DQWSPALTIS
110 120 130 140
KVLLSICSLL TDPNPDDPLV PEIAHVYKTD RSRYELSARE WTRKYAI
Length:147
Mass (Da):16,476
Last modified:November 1, 1995 - v1
Checksum:i3E8BADFF494A91EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L37384 mRNA. No translation available.
CU329671 Genomic DNA. Translation: CAA17917.1.
PIRiT39300.
RefSeqiNP_595283.1. NM_001021190.2.

Genome annotation databases

EnsemblFungiiSPBC119.02.1; SPBC119.02.1:pep; SPBC119.02.
GeneIDi2540083.
KEGGispo:SPBC119.02.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L37384 mRNA. No translation available.
CU329671 Genomic DNA. Translation: CAA17917.1.
PIRiT39300.
RefSeqiNP_595283.1. NM_001021190.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4II2X-ray2.20C1-147[»]
ProteinModelPortaliP46595.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276621. 17 interactors.
IntActiP46595. 1 interactor.
MINTiMINT-4690340.

Proteomic databases

MaxQBiP46595.
PRIDEiP46595.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC119.02.1; SPBC119.02.1:pep; SPBC119.02.
GeneIDi2540083.
KEGGispo:SPBC119.02.

Organism-specific databases

EuPathDBiFungiDB:SPBC119.02.
PomBaseiSPBC119.02. ubc4.

Phylogenomic databases

HOGENOMiHOG000233455.
InParanoidiP46595.
KOiK06689.
OMAiLYKTDRT.
OrthoDBiEOG092C50OL.
PhylomeDBiP46595.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-SPO-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.

Miscellaneous databases

PROiP46595.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBC4_SCHPO
AccessioniPrimary (citable) accession number: P46595
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.