Reviewed, UniProtKB/Swiss-Prot P46594 (HAL21_CANAL)

Names and origin

Protein names

Recommended name:
    3'(2'),5'-bisphosphate nucleotidase 1
    EC=3.1.3.7
Alternative name(s):
    3'(2'),5-bisphosphonucleoside 3'(2')-phosphohydrolase 1
    DPNPase 1
    Halotolerance protein HAL21

Gene names

Name:	HAL21
Synonyms:	MET222
ORF Names:	CaO19.99, CaO19.7746

Organism

Candida albicans (Yeast)

Taxonomic identifier

5476 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'- phosphate (PAP) to AMP. Regulates the flux of sulfur in the sulfur-activation pathway by converting PAPS to APS. Involved in salt tolerance By similarity.
Catalytic activity	Adenosine 3',5'-bisphosphate + H2O = adenosine 5'-phosphate + phosphate.
Cofactor	Magnesium By similarity.
Sequence similarities	Belongs to the inositol monophosphatase family.
Sequence caution	The sequence EAK95236.1 differs from that shown. Reason: Frameshift at position 377.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW sulfur metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	3'(2'),5'-bisphosphate nucleotidase activity Inferred from electronic annotation. Source: EC inositol or phosphatidylinositol phosphatase activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	3'(2'),5'-bisphosphate nucleotidase 1		PRO_0000142538
Sites
	Metal binding	77	1	Magnesium 1 By similarity
	Metal binding	141	1	Magnesium 1 By similarity
	Metal binding	141	1	Magnesium 2 By similarity
	Metal binding	143	1	Magnesium 1; via carbonyl oxygen By similarity
	Metal binding	144	1	Magnesium 2 By similarity
	Metal binding	302	1	Magnesium 2 By similarity
Natural variations
	Natural variant	213	1	T → A in allele CaO19.7746.
	Natural variant	260 – 261	2	PT → SA in allele CaO19.7746.
	Natural variant	323	1	A → S in allele CaO19.7746 and in strain: WO-1.
	Natural variant	334	1	N → D in allele CaO19.7746.
	Natural variant	347	1	D → N in allele CaO19.7746.
	Natural variant	356	1	I → V in allele CaO19.7746.
	Natural variant	358	1	K → N in allele CaO19.7746.
	Natural variant	363	1	R → K in allele CaO19.7746.

Sequences

	Sequence		Length	Mass (Da)
	P46594-1 [UniParc]. Last modified July 11, 2006. Version 2. Checksum: 0EC8BF70CAD10E19	FASTA	364	39,310
	10 20 30 40 50 60 MSHTTHPYQK ELEVATLAVK RASLLTKQLS DSIVQTARSG TLTKDDKSPV TIGDFASQAI 70 80 90 100 110 120 INHAIKLNFP SDEIVGEEDS QELQENSSLA DQVLSLIIKI QQETSVYNDV VGTLTDKNKV 130 140 150 160 170 180 FQSIDYGNSQ GGSKGRFWAL DPIDGTKGFL RGDQFAVCLA LIEDGKVVLG VIGCPNLSEN 190 200 210 220 230 240 IVSNEEHSGV VGGLYSAVKG VGSFYSELFK EGTEPLSQQK PIKMQNHTNP SQLKVVEGVE 250 260 270 280 290 300 KGHSSHSTQA EIKAKLGFDP TTVAKQTVNL DSQVKYCVLA SGQADIYLRL PVSDTYREKI 310 320 330 340 350 360 WDHAAGNILI YESGGQVGDV TGAPLNFGNG RTLNSKGVIA ANKEIFDKVI DAVTEIRKSS TPRV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed: 15123810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314.
[2]	"CAN1, a gene encoding a permease for basic amino acids in Candida albicans." Sychorova H., Souciet J.-L. Yeast 10:1647-1651(1994) [PubMed: 7725800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 260-364. Strain: WO-1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AACQ01000106 Genomic DNA. Translation: EAK95236.1. Sequence problems. AACQ01000112 Genomic DNA. Translation: EAK94936.1. Different initiation. X76689 Genomic DNA. No translation available.
RefSeq	XP_714033.1. XP_714308.1.
3D structure databases
HSSP	HSSP built from PDB template 2HHM based on UniProtKB P29218.
ModBase	Search...
Genome annotation databases
GeneID	3644045. 3644303.
KEGG	cal:CaO19.7746. cal:CaO19.99.
Enzyme and pathway databases
BRENDA	3.1.3.7. 1124.
Family and domain databases
InterPro	IPR006239. Bisphos_HAL2. IPR000760. Inositol_P. [Graphical view]
PANTHER	PTHR20854. Inositol_P. 1 hit.
Pfam	PF00459. Inositol_P. 1 hit. [Graphical view]
PRINTS	PR00378. INOSPHPHTASE.
TIGRFAMs	TIGR01330. bisphos_HAL2. 1 hit.
PROSITE	PS00629. IMP_1. 1 hit. PS00630. IMP_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HAL21_CANAL

Accession

Primary (citable) accession number: P46594
Secondary accession number(s): Q59WV3, Q59XQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	July 11, 2006
Last modified:	June 16, 2009
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)