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P46593

- HWP1_CANAL

UniProt

P46593 - HWP1_CANAL

Protein

Hyphal wall protein 1

Gene

HWP1

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 4 (19 Oct 2011)
      Previous versions | rss
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    Functioni

    Major hyphal cell wall protein which plays a role of adhesin and is required for mating, normal hyphal development, cell-to-cell adhesive functions necessary for biofilm integrity, attachment to host, and virulence. Promotes interactions with host and bacterial molecules, thus leading to effective colonization within polymicrobial communities. Plays a crucial role in gastrointestinal colonization, in mucosal symptomatic and asymptomatic infections, in vaginitis, as well as in lethal oroesophageal candidiasis, caused by the combined action of fungal virulence factors and host inflammatory responses when protective immunity is absent.16 Publications

    GO - Molecular functioni

    1. cell adhesion molecule binding Source: CGD

    GO - Biological processi

    1. adhesion of symbiont to host Source: CGD
    2. cell adhesion Source: CGD
    3. cell adhesion involved in biofilm formation Source: CGD
    4. cell adhesion involved in single-species biofilm formation in or on host organism Source: CGD
    5. cellular response to drug Source: CGD
    6. filamentous growth Source: CGD
    7. fungal-type cell wall organization Source: CGD
    8. growth of symbiont in host Source: CGD
    9. pathogenesis Source: CGD
    10. single organismal cell-cell adhesion Source: CGD
    11. single-species biofilm formation in or on host organism Source: CGD
    12. single-species biofilm formation on inanimate substrate Source: CGD

    Keywords - Biological processi

    Cell adhesion, Virulence

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hyphal wall protein 1
    Alternative name(s):
    Cell elongation protein 2
    Gene namesi
    Name:HWP1
    Synonyms:ECE2
    ORF Names:CaO19.1321, CaO19.8901
    OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
    Taxonomic identifieri237561 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida
    ProteomesiUP000000559: Unassembled WGS sequence

    Organism-specific databases

    CGDiCAL0003753. HWP1.

    Subcellular locationi

    Secretedcell wall. Membrane; Lipid-anchorGPI-anchor
    Note: Hyphal surface. Localizes to the a/a Portion of the conjugation bridge during mating.

    GO - Cellular componenti

    1. anchored component of plasma membrane Source: CGD
    2. cell surface Source: CGD
    3. extracellular region Source: CGD
    4. fungal-type cell wall Source: CGD
    5. hyphal cell wall Source: CGD
    6. mating projection Source: CGD

    Keywords - Cellular componenti

    Cell wall, Membrane, Secreted

    Pathology & Biotechi

    Disruption phenotypei

    Reduces the overall mating frequency in both liquid and solid media. Impairs stable attachments to human buccal epithelial cells and reduces capacity to cause systemic candidiasis in mice. Produces a thin biofilm that lacks much of the hyphal mass found in the wild type cell.6 Publications

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi613 – 6131G → D, N or S: Still allows localization to the cell surface. 1 Publication
    Mutagenesisi613 – 6131G → E, Q, R or T: Impairs cell surface localization and localizes exclusively to internal ER-like structures. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Chaini28 – 613586Hyphal wall protein 1PRO_0000084097Add
    BLAST
    Propeptidei614 – 63421Removed in mature formPRO_0000425596Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi241 – 2411N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi286 – 2861N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi601 – 6011N-linked (GlcNAc...)Sequence Analysis
    Lipidationi613 – 6131GPI-anchor amidated glycine1 Publication

    Post-translational modificationi

    The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.
    Serves as a substrate for mammalian transglutaminases which are necessary for cross-linking between HWP1 and host epithelial cells. Also predicted to be a substrate for cleavage by KEX2.
    N- and O-glycosylated.2 Publications

    Keywords - PTMi

    Glycoprotein, GPI-anchor, Lipoprotein

    Expressioni

    Developmental stagei

    Found in hyphal but not yeast forms.

    Inductioni

    The promoter contains a 368 bp region, the HWP1 control region (HCR), which is target of transcription factors and is critical for activation under hypha-inducing conditions. Expression is positively regulated by BCR1, EFG1, FLO8, GPA2, GPR1, MSS11, RBF1, and RFG1; and negatively regulated by NRG1 and TUP1. Transcription is induced in an adhesion-dependent manner and in presence of human serum or hemoglobin. Expression is down-regulated by hypoxic conditions, allicine, ethylenediaminetetra-acetic acid (EDTA), farnesol, linalool, riccardin D, purpurin, filastatin, phorbasin H, Ocimum sanctum essential oil (OSEO), as well as many substances belonging to chemical classes such as methyl aryl-oxazoline carboxylates, dihydrobenzo-d-isoxazolones, and thiazolo-4,5-e-benzoisoxazoles. Moreover, the competitors Saccharomyces boulardii or Streptococcus mutans secrete respectively capric acid and trans-2-decenoic acid (SDSF) which also suppress HWP1 expression.35 Publications

    Interactioni

    Protein-protein interaction databases

    STRINGi5476.CAL0003753.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati46 – 58131; approximateAdd
    BLAST
    Repeati59 – 69112; approximateAdd
    BLAST
    Repeati70 – 81123; approximateAdd
    BLAST
    Repeati82 – 91104
    Repeati92 – 101105
    Repeati102 – 111106
    Repeati112 – 121107
    Repeati122 – 131108
    Repeati132 – 141109
    Repeati142 – 1511010
    Repeati152 – 1611011
    Repeati162 – 1711012
    Repeati172 – 179813; truncated
    Repeati180 – 187814; truncated

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni46 – 18714214 X 10 AA tandem repeats of [EVIQ]-P-[CDT]-D-[YNW]-P-[PQ]-[QI]-[QP]-[QDN]Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi53 – 586Poly-Gln
    Compositional biasi65 – 695Poly-Gln
    Compositional biasi204 – 2074Poly-Thr
    Compositional biasi208 – 22013Poly-SerAdd
    BLAST
    Compositional biasi291 – 2944Poly-Thr
    Compositional biasi304 – 3074Poly-Thr
    Compositional biasi398 – 4014Poly-Val

    Sequence similaritiesi

    Belongs to the HWP1 family.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG12793.
    OrthoDBiEOG718KPF.

    Family and domain databases

    InterProiIPR025928. Flocculin_t3_rpt.
    [Graphical view]
    PfamiPF13928. Flocculin_t3. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P46593-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRLSTAQLIA IAYYMLSIGA TVPQVDGQGE TEEALIQKRS YDYYQEPCDD    50
    YPQQQQQQEP CDYPQQQQQE EPCDYPQQQP QEPCDYPQQP QEPCDYPQQP 100
    QEPCDYPQQP QEPCDNPPQP DVPCDNPPQP DVPCDNPPQP DIPCDNPPQP 150
    DIPCDNPPQP DQPDDNPPIP NIPTDWIPNI PTDWIPDIPE KPTTPATTPN 200
    IPATTTTSES SSSSSSSSSS TTPKTSASTT PESSVPATTP NTSVPTTSSE 250
    STTPATSPES SVPVTSGSSI LATTSESSSA PATTPNTSVP TTTTEAKSSS 300
    TPLTTTTEHD TTVVTVTSCS NSVCTESEVT TGVIVITSKD TIYTTYCPLT 350
    ETTPVSTAPA TETPTGTVST STEQSTTVIT VTSCSESSCT ESEVTTGVVV 400
    VTSEETVYTT FCPLTENTPG TDSTPEASIP PMETIPAGSE SSMPAGETSP 450
    AVPKSDVPAT ESAPVPEMTP AGSQPSIPAG ETSPAVPKSD VPATESAPAP 500
    EMTPAGTETK PAAPKSSAPA TEPSPVAPGT ESAPAGPGAS SSPKSSVLAS 550
    ETSPIAPGAE TAPAGSSGAI TIPESSAVVS TTEGAIPTTL ESVPLMQPSA 600
    NYSSVAPIST FEGAGNNMRL TFGAAIIGIA AFLI 634
    Length:634
    Mass (Da):65,342
    Last modified:October 19, 2011 - v4
    Checksum:i05B7ACBD44D7ADF5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti141 – 1411D → DVPCDNPPQPD in AAC49209. (PubMed:8626424)Curated
    Sequence conflicti296 – 2961A → T in AAC96368. (PubMed:9639315)Curated
    Sequence conflicti441 – 4411S → P in AAB64014. (PubMed:10464197)Curated
    Sequence conflicti492 – 4921P → S in AAB64014. (PubMed:10464197)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U64206 Genomic DNA. Translation: AAC96368.1.
    AF001978 Genomic DNA. Translation: AAB64014.1.
    AACQ01000139 Genomic DNA. Translation: EAK93836.1.
    AACQ01000138 Genomic DNA. Translation: EAK93868.1.
    U29369 mRNA. Translation: AAC49209.1.
    RefSeqiXP_709961.1. XM_704869.1.
    XP_712998.1. XM_707905.1.

    Genome annotation databases

    GeneIDi3645372.
    3645386.
    KEGGical:CaO19.1321.
    cal:CaO19.8901.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U64206 Genomic DNA. Translation: AAC96368.1 .
    AF001978 Genomic DNA. Translation: AAB64014.1 .
    AACQ01000139 Genomic DNA. Translation: EAK93836.1 .
    AACQ01000138 Genomic DNA. Translation: EAK93868.1 .
    U29369 mRNA. Translation: AAC49209.1 .
    RefSeqi XP_709961.1. XM_704869.1.
    XP_712998.1. XM_707905.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5476.CAL0003753.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3645372.
    3645386.
    KEGGi cal:CaO19.1321.
    cal:CaO19.8901.

    Organism-specific databases

    CGDi CAL0003753. HWP1.

    Phylogenomic databases

    eggNOGi NOG12793.
    OrthoDBi EOG718KPF.

    Family and domain databases

    InterProi IPR025928. Flocculin_t3_rpt.
    [Graphical view ]
    Pfami PF13928. Flocculin_t3. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genetic organization and sequence analysis of the hypha-specific cell wall protein gene HWP1 of Candida albicans."
      Staab J.F., Sundstrom P.
      Yeast 14:681-686(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: SC5314 / ATCC MYA-2876.
    2. "HWP1 functions in the morphological development of Candida albicans downstream of EFG1, TUP1, and RBF1."
      Sharkey L.L., McNemar M.D., Saporito-Irwin S.M., Sypherd P.S., Fonzi W.A.
      J. Bacteriol. 181:5273-5279(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION.
      Strain: SC5314 / ATCC MYA-2876.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SC5314 / ATCC MYA-2876.
    4. "Developmental expression of a tandemly repeated, proline- and glutamine-rich amino acid motif on hyphal surfaces on Candida albicans."
      Staab J.F., Ferrer C.A., Sundstrom P.
      J. Biol. Chem. 271:6298-6305(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-225, INDUCTION, SUBCELLULAR LOCATION.
      Strain: SC5314 / ATCC MYA-2876.
    5. "Adhesive and mammalian transglutaminase substrate properties of Candida albicans Hwp1."
      Staab J.F., Bradway S.D., Fidel P.L., Sundstrom P.
      Science 283:1535-1538(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    6. "TUP1, CPH1 and EFG1 make independent contributions to filamentation in Candida albicans."
      Braun B.R., Johnson A.D.
      Genetics 155:57-67(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    7. "Identification and characterization of TUP1-regulated genes in Candida albicans."
      Braun B.R., Head W.S., Wang M.X., Johnson A.D.
      Genetics 156:31-44(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    8. "Reduced virulence of HWP1-deficient mutants of Candida albicans and their interactions with host cells."
      Tsuchimori N., Sharkey L.L., Fonzi W.A., French S.W., Edwards J.E. Jr., Filler S.G.
      Infect. Immun. 68:1997-2002(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    9. "Efg1, a morphogenetic regulator in Candida albicans, is a sequence-specific DNA binding protein."
      Leng P., Lee P.R., Wu H., Brown A.J.
      J. Bacteriol. 183:4090-4093(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    10. "Rfg1, a protein related to the Saccharomyces cerevisiae hypoxic regulator Rox1, controls filamentous growth and virulence in Candida albicans."
      Kadosh D., Johnson A.D.
      Mol. Cell. Biol. 21:2496-2505(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    11. "Transcript profiling in Candida albicans reveals new cellular functions for the transcriptional repressors CaTup1, CaMig1 and CaNrg1."
      Murad A.M., d'Enfert C., Gaillardin C., Tournu H., Tekaia F., Talibi D., Marechal D., Marchais V., Cottin J., Brown A.J.
      Mol. Microbiol. 42:981-993(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    12. "Inhibition of Candida albicans biofilm formation by farnesol, a quorum-sensing molecule."
      Ramage G., Saville S.P., Wickes B.L., Lopez-Ribot J.L.
      Appl. Environ. Microbiol. 68:5459-5463(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    13. "Reevaluation of the role of HWP1 in systemic candidiasis by use of Candida albicans strains with selectable marker URA3 targeted to the ENO1 locus."
      Sundstrom P., Cutler J.E., Staab J.F.
      Infect. Immun. 70:3281-3283(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    14. "Inactivation of Kex2p diminishes the virulence of Candida albicans."
      Newport G., Kuo A., Flattery A., Gill C., Blake J.J., Kurtz M.B., Abruzzo G.K., Agabian N.
      J. Biol. Chem. 278:1713-1720(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PREDICTION AS A SUBSTRATE FOR KEX2 CLEAVAGE.
    15. "Essential role of the Candida albicans transglutaminase substrate, hyphal wall protein 1, in lethal oroesophageal candidiasis in immunodeficient mice."
      Sundstrom P., Balish E., Allen C.M.
      J. Infect. Dis. 185:521-530(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    16. "The adhesin Hwp1 and the first daughter cell localize to the a/a portion of the conjugation bridge during Candida albicans mating."
      Daniels K.J., Lockhart S.R., Staab J.F., Sundstrom P., Soll D.R.
      Mol. Biol. Cell 14:4920-4930(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    17. "Use of green fluorescent protein fusions to analyse the N- and C-terminal signal peptides of GPI-anchored cell wall proteins in Candida albicans."
      Mao Y., Zhang Z., Wong B.
      Mol. Microbiol. 50:1617-1628(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: GPI-ANCHOR AT GLY-613, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-613.
    18. "Genome-wide identification of fungal GPI proteins."
      De Groot P.W., Hellingwerf K.J., Klis F.M.
      Yeast 20:781-796(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PREDICTION OF GPI-ANCHOR.
    19. "Gpr1, a putative G-protein-coupled receptor, regulates morphogenesis and hypha formation in the pathogenic fungus Candida albicans."
      Miwa T., Takagi Y., Shinozaki M., Yun C.W., Schell W.A., Perfect J.R., Kumagai H., Tamaki H.
      Eukaryot. Cell 3:919-931(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    20. "Expression of transglutaminase substrate activity on Candida albicans germ tubes through a coiled, disulfide-bonded N-terminal domain of Hwp1 requires C-terminal glycosylphosphatidylinositol modification."
      Staab J.F., Bahn Y.S., Tai C.H., Cook P.F., Sundstrom P.
      J. Biol. Chem. 279:40737-40747(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, GLYCOSYLATION, GPI-ANCHOR, CELL WALL ANCHORAGE.
    21. "Regulation of cell-surface genes and biofilm formation by the C. albicans transcription factor Bcr1p."
      Nobile C.J., Mitchell A.P.
      Curr. Biol. 15:1150-1155(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    22. "Function of Candida albicans adhesin Hwp1 in biofilm formation."
      Nobile C.J., Nett J.E., Andes D.R., Mitchell A.P.
      Eukaryot. Cell 5:1604-1610(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    23. "An in vitro assay to study the transcriptional response during adherence of Candida albicans to different human epithelia."
      Sohn K., Senyurek I., Fertey J., Konigsdorfer A., Joffroy C., Hauser N., Zelt G., Brunner H., Rupp S.
      FEMS Yeast Res. 6:1085-1093(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    24. "Candida albicans HWP1 gene expression and host antibody responses in colonization and disease."
      Naglik J.R., Fostira F., Ruprai J., Staab J.F., Challacombe S.J., Sundstrom P.
      J. Med. Microbiol. 55:1323-1327(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.
    25. "Expression of firefly luciferase in Candida albicans and its use in the selection of stable transformants."
      Doyle T.C., Nawotka K.A., Purchio A.F., Akin A.R., Francis K.P., Contag P.R.
      Microb. Pathog. 40:69-81(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    26. "The Flo8 transcription factor is essential for hyphal development and virulence in Candida albicans."
      Cao F., Lane S., Raniga P.P., Lu Y., Zhou Z., Ramon K., Chen J., Liu H.
      Mol. Biol. Cell 17:295-307(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    27. "Critical role of Bcr1-dependent adhesins in C. albicans biofilm formation in vitro and in vivo."
      Nobile C.J., Andes D.R., Nett J.E., Smith F.J., Yue F., Phan Q.T., Edwards J.E., Filler S.G., Mitchell A.P.
      PLoS Pathog. 2:E63-E63(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    28. "Diagnosis of invasive candidiasis by enzyme-linked immunosorbent assay using the N-terminal fragment of Candida albicans hyphal wall protein 1."
      Lain A., Elguezabal N., Brena S., Garcia-Ruiz J.C., Del Palacio A., Moragues M.D., Ponton J.
      BMC Microbiol. 7:35-35(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: DIAGNOSTIC TOOL.
    29. "A 368-base-pair cis-acting HWP1 promoter region, HCR, of Candida albicans confers hypha-specific gene regulation and binds architectural transcription factors Nhp6 and Gcf1p."
      Kim S., Wolyniak M.J., Staab J.F., Sundstrom P.
      Eukaryot. Cell 6:693-709(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    30. "State of differentiation defines buccal epithelial cell affinity for cross-linking to Candida albicans Hwp1."
      Ponniah G., Rollenhagen C., Bahn Y.S., Staab J.F., Sundstrom P.
      J. Oral Pathol. Med. 36:456-467(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBSTRATE FOR HOST TRANSGLUTAMINASES.
    31. "Hemoglobin is an effective inducer of hyphal differentiation in Candida albicans."
      Pendrak M.L., Roberts D.D.
      Med. Mycol. 45:61-71(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    32. "Inhibition on Candida albicans biofilm formation using divalent cation chelators (EDTA)."
      Ramage G., Wickes B.L., Lopez-Ribot J.L.
      Mycopathologia 164:301-306(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    33. "Candida albicans Tup1 is involved in farnesol-mediated inhibition of filamentous-growth induction."
      Kebaara B.W., Langford M.L., Navarathna D.H., Dumitru R., Nickerson K.W., Atkin A.L.
      Eukaryot. Cell 7:980-987(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    34. "C-terminal signals regulate targeting of glycosylphosphatidylinositol-anchored proteins to the cell wall or plasma membrane in Candida albicans."
      Mao Y., Zhang Z., Gast C., Wong B.
      Eukaryot. Cell 7:1906-1915(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, GPI-ANCHOR.
    35. "Expression of ALS1, HWP1 and SAP4 genes in Candida albicans strains isolated from women with vaginitis."
      Nas T., Kalkanci A., Fidan I., Hizel K., Bolat S., Yolbakan S., Yilmaz E., Ozkan S., Kustimur S.
      Folia Microbiol. (Praha) 53:179-183(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.
    36. "Functional analysis of Candida albicans GPI-anchored proteins: roles in cell wall integrity and caspofungin sensitivity."
      Plaine A., Walker L., Da Costa G., Mora-Montes H.M., McKinnon A., Gow N.A., Gaillardin C., Munro C.A., Richard M.L.
      Fungal Genet. Biol. 45:1404-1414(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    37. "Beta-1,2 oligomannose adhesin epitopes are widely distributed over the different families of Candida albicans cell wall mannoproteins and are associated through both N- and O-glycosylation processes."
      Fradin C., Slomianny M.C., Mille C., Masset A., Robert R., Sendid B., Ernst J.F., Michalski J.C., Poulain D.
      Infect. Immun. 76:4509-4517(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION.
    38. "Hypoxic conditions and iron restriction affect the cell-wall proteome of Candida albicans grown under vagina-simulative conditions."
      Sosinska G.J., de Groot P.W., Teixeira de Mattos M.J., Dekker H.L., de Koster C.G., Hellingwerf K.J., Klis F.M.
      Microbiology 154:510-520(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INDUCTION.
    39. "Synthetic glycopeptide vaccines combining beta-mannan and peptide epitopes induce protection against candidiasis."
      Xin H., Dziadek S., Bundle D.R., Cutler J.E.
      Proc. Natl. Acad. Sci. U.S.A. 105:13526-13531(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRODUCTION OF VACCINES.
    40. "The inhibitory effect of a macrocyclic bisbibenzyl riccardin D on the biofilms of Candida albicans."
      Cheng A., Sun L., Wu X., Lou H.
      Biol. Pharm. Bull. 32:1417-1421(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    41. "Mss11, a transcriptional activator, is required for hyphal development in Candida albicans."
      Su C., Li Y., Lu Y., Chen J.
      Eukaryot. Cell 8:1780-1791(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    42. "Hwp1 and related adhesins contribute to both mating and biofilm formation in Candida albicans."
      Ene I.V., Bennett R.J.
      Eukaryot. Cell 8:1909-1913(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    43. "Trifluoromethanesulfonic acid-based proteomic analysis of cell wall and secreted proteins of the ascomycetous fungi Neurospora crassa and Candida albicans."
      Maddi A., Bowman S.M., Free S.J.
      Fungal Genet. Biol. 46:768-781(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, INDUCTION.
    44. "Interaction of Candida albicans biofilms with antifungals: transcriptional response and binding of antifungals to beta-glucans."
      Vediyappan G., Rossignol T., d'Enfert C.
      Antimicrob. Agents Chemother. 54:2096-2111(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    45. "Heterologous expression of Candida albicans cell wall-associated adhesins in Saccharomyces cerevisiae Reveals differential specificities in adherence and biofilm formation and in binding oral Streptococcus gordonii."
      Nobbs A.H., Vickerman M.M., Jenkinson H.F.
      Eukaryot. Cell 9:1622-1634(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    46. "Streptococcus mutans inhibits Candida albicans hyphal formation by the fatty acid signaling molecule trans-2-decenoic acid (SDSF)."
      Vilchez R., Lemme A., Ballhausen B., Thiel V., Schulz S., Jansen R., Sztajer H., Wagner-Dobler I.
      ChemBioChem 11:1552-1562(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    47. "Capric acid secreted by S. boulardii inhibits C. albicans filamentous growth, adhesion and biofilm formation."
      Murzyn A., Krasowska A., Stefanowicz P., Dziadkowiec D., Lukaszewicz M.
      PLoS ONE 5:E12050-E12050(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    48. "Mass spectrometric quantification of the adaptations in the wall proteome of Candida albicans in response to ambient pH."
      Sosinska G.J., de Koning L.J., de Groot P.W., Manders E.M., Dekker H.L., Hellingwerf K.J., de Koster C.G., Klis F.M.
      Microbiology 157:136-146(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INDUCTION.
    49. "Comparison between allicin and fluconazole in Candida albicans biofilm inhibition and in suppression of HWP1 gene expression."
      Khodavandi A., Harmal N.S., Alizadeh F., Scully O.J., Sidik S.M., Othman F., Sekawi Z., Ng K.P., Chong P.P.
      Phytomedicine 19:56-63(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    50. "Role of Bcr1-activated genes Hwp1 and Hyr1 in Candida albicans oral mucosal biofilms and neutrophil evasion."
      Dwivedi P., Thompson A., Xie Z., Kashleva H., Ganguly S., Mitchell A.P., Dongari-Bagtzoglou A.
      PLoS ONE 6:E16218-E16218(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    51. "Purpurin suppresses Candida albicans biofilm formation and hyphal development."
      Tsang P.W., Bandara H.M., Fong W.P.
      PLoS ONE 7:E50866-E50866(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    52. "Inhibition of yeast-to-hypha transition in Candida albicans by phorbasin H isolated from Phorbas sp."
      Lee S.H., Jeon J.E., Ahn C.H., Chung S.C., Shin J., Oh K.B.
      Appl. Microbiol. Biotechnol. 97:3141-3148(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    53. "Identification of inhibitors of yeast-to-hyphae transition in Candida albicans by a reporter screening assay."
      Heintz-Buschart A., Eickhoff H., Hohn E., Bilitewski U.
      J. Biotechnol. 164:137-142(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    54. "The inhibitory activity of linalool against the filamentous growth and biofilm formation in Candida albicans."
      Hsu C.C., Lai W.L., Chuang K.C., Lee M.H., Tsai Y.C.
      Med. Mycol. 51:473-482(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    55. "Ocimum sanctum essential oil inhibits virulence attributes in Candida albicans."
      Khan A., Ahmad A., Xess I., Khan L.A., Manzoor N.
      Phytomedicine 21:448-452(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    56. "Human serum promotes Candida albicans biofilm growth and virulence gene expression on silicone biomaterial."
      Samaranayake Y.H., Cheung B.P., Yau J.Y., Yeung S.K., Samaranayake L.P.
      PLoS ONE 8:E62902-E62902(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    57. "Niche-specific requirement for hyphal wall protein 1 in virulence of Candida albicans."
      Staab J.F., Datta K., Rhee P.
      PLoS ONE 8:E80842-E80842(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    58. "Chemical screening identifies filastatin, a small molecule inhibitor of Candida albicans adhesion, morphogenesis, and pathogenesis."
      Fazly A., Jain C., Dehner A.C., Issi L., Lilly E.A., Ali A., Cao H., Fidel P.L. Jr., Rao R.P., Kaufman P.D.
      Proc. Natl. Acad. Sci. U.S.A. 110:13594-13599(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.

    Entry informationi

    Entry nameiHWP1_CANAL
    AccessioniPrimary (citable) accession number: P46593
    Secondary accession number(s): O13424, P87019, Q59TL7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: October 19, 2011
    Last modified: October 1, 2014
    This is version 57 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    As a major cell surface protein expressed during infection, HWP1 detection can be used for diagnosis of invasive candidiasis. HWP1 epitopes can also be used to develop vaccines for protection against candidiasis.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Candida albicans
      Candida albicans: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3