P46579 (SYWM_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan--tRNA ligase, mitochondrial
EC=6.1.1.2

Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS

Gene names

Name:	wars-2
Synonyms:	wrs-2
ORF Names:	C34E10.4

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.
Sequence caution	The sequence CCD66656.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tryptophanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular_component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tryptophan-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 360		Tryptophan--tRNA ligase, mitochondrial		PRO_0000136746
Regions
	Motif	39 – 47	9	"HIGH" region
	Motif	229 – 233	5	"KMSKS" region
Sites
	Binding site	232	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P46579 [UniParc]. Last modified January 25, 2012. Version 3. Checksum: E73230D906FA1E7A	FASTA	360	40,474
	10 20 30 40 50 60 MIFSGKFTSH LLNYGFKPNN LRLLSTSTHP TIYFTGIQPT GIPHLGNFFG SIEPWTELQN 70 80 90 100 110 120 SVDKNILMML SVVDQHAISL GPLPANELRQ NTHQMTASLI ACGVDPNRTL LFRQSDVPQI 130 140 150 160 170 180 AQISWILGSL QTTSKLARLP QYKEKKERFK KGDIPVGLLT YPLLQAADVL TFKATTVPVG 190 200 210 220 230 240 EDQSQHLNLL GGLAYAFNKT YETEIFPIPK QLTRESHARI RSLREPEKKM SKSSGGPRSR 250 260 270 280 290 300 IEITDSRSTI IEKCQKAQSD NAGKVTYDKE NRLAVSNLLD LYSAVTKTQT SEIDFSNWTT 310 320 330 340 350 360 LDLKMNLAEA VDKRLAPIRQ KFEELQNTGE VDKVLTENGE KAREIAEKNL EEIRRTIGFL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"C. elegans mitochondrial aminoacyl-tRNA synthetases." Ohtsuki T. Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO080774 Genomic DNA. Translation: CCD66648.1. FO080774 Genomic DNA. Translation: CCD66656.1. Sequence problems. AB096634 mRNA. Translation: BAC76734.1.
PIR	T15761.
RefSeq	NP_001021200.2. NM_001026029.3. NP_001021201.1. NM_001026030.3.
UniGene	Cel.23360.
3D structure databases
ProteinModelPortal	P46579.
SMR	P46579. Positions 34-359.
ModBase	Search...
Protein-protein interaction databases
IntAct	P46579. 1 interaction.
STRING	6239.C34E10.4a.
Proteomic databases
PaxDb	P46579.
PRIDE	P46579.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	C34E10.4b; C34E10.4b; C34E10.4.
GeneID	175719.
KEGG	cel:CELE_C34E10.4.
UCSC	C34E10.4a. c. elegans.
Organism-specific databases
CTD	175719.
WormBase	C34E10.4a; CE29032; WBGene00006946; wars-2. C34E10.4b; CE37710; WBGene00006946; wars-2.
Phylogenomic databases
eggNOG	COG5574.
GeneTree	ENSGT00510000047425.
HOGENOM	HOG000022014.
InParanoid	P46579.
OMA	KFAPIKS.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002306. Trp-tRNA-ligase. [Graphical view]
PANTHER	PTHR10055. PTHR10055. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01039. TRNASYNTHTRP.
TIGRFAMs	TIGR00233. trpS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	889350.

Entry information

Entry name

SYWM_CAEEL

Accession

Primary (citable) accession number: P46579
Secondary accession number(s): Q5TYM1, Q7Z259

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 25, 2012
Last modified:	May 1, 2013
This is version 102 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project