Reviewed, UniProtKB/Swiss-Prot P46573 (APK1B_ARATH)

Names and origin

Protein names

Recommended name:
    Protein kinase APK1B, chloroplastic
    EC=2.7.11.1

Gene names

Name:	APK1B
Ordered Locus Names:	At2g28930
ORF Names:	T9I4.1

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Possible bi-functional kinase. In vitro, it exhibits serine/threonine activity. In vivo, can phosphorylate tyrosine residues of limited substrates By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Plastid › chloroplast Potential.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P46573-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 59	59	Chloroplast Potential
	Chain	60 – 412	353	Protein kinase APK1B, chloroplastic		PRO_0000024303
Regions
	Domain	69 – 356	288	Protein kinase
	Nucleotide binding	75 – 83	9	ATP By similarity
Sites
	Active site	204	1	Proton acceptor By similarity
	Binding site	107	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: EB1CA0B1A626A5DA	FASTA	412	45,746
	10 20 30 40 50 60 MGICLSAQIK AVSPGASPKY MSSEANDSLG SKSSSVSIRT NPRTEGEILQ SPNLKSFTFA 70 80 90 100 110 120 ELKAATRNFR PDSVLGEGGF GSVFKGWIDE QTLTASKPGT GVVIAVKKLN QDGWQGHQEW 130 140 150 160 170 180 LAEVNYLGQF SHPNLVKLIG YCLEDEHRLL VYEFMPRGSL ENHLFRRGSY FQPLSWTLRL 190 200 210 220 230 240 KVALGAAKGL AFLHNAETSV IYRDFKTSNI LLDSEYNAKL SDFGLAKDGP TGDKSHVSTR 250 260 270 280 290 300 IMGTYGYAAP EYLATGHLTT KSDVYSYGVV LLEVLSGRRA VDKNRPPGEQ KLVEWARPLL 310 320 330 340 350 360 ANKRKLFRVI DNRLQDQYSM EEACKVATLA LRCLTFEIKL RPNMNEVVSH LEHIQTLNEA 370 380 390 400 410 GGRNIDMVQR RMRRRSDSVA INQKPNAGFA RQTAVGVIAT AYPRPSDSPL FV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Novel protein kinase of Arabidopsis thaliana (APK1) that phosphorylates tyrosine, serine and threonine." Hirayama T., Oka A. Plant Mol. Biol. 20:653-662(1992) [PubMed: 1450380] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 143-346. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC005315 Genomic DNA. Translation: AAC33221.1. D10152 mRNA. Translation: BAA20968.1.
IPI	IPI00655414.
PIR	T02725.
RefSeq	NP_001031439.1.
UniGene	At.46050
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P46573.
Genome annotation databases
GeneID	817442.
GenomeReviews	Gene locus AT2G28930 in contig CT485783_GR.
KEGG	ath:AT2G28930.
Organism-specific databases
GeneFarm	1774. 129.
TAIR	At2g28930.
Enzyme and pathway databases
BRENDA	2.7.10.2. 302. 2.7.11.1. 302.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

APK1B_ARATH

Accession

Primary (citable) accession number: P46573
Secondary accession number(s): Q9SLH5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	December 1, 2000
Last modified:	June 16, 2009
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)