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Protein

Protein kinase APK1B, chloroplastic

Gene

APK1B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Possible bi-functional kinase. In vitro, it exhibits serine/threonine activity. In vivo, can phosphorylate tyrosine residues of limited substrates (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei107 – 1071ATPPROSITE-ProRule annotation
Active sitei204 – 2041Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi75 – 839ATPPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G28930-MONOMER.
ARA:GQT-1640-MONOMER.
ARA:GQT-2470-MONOMER.
BRENDAi2.7.10.2. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase APK1B, chloroplastic (EC:2.7.11.1)
Gene namesi
Name:APK1B
Ordered Locus Names:At2g28930
ORF Names:T9I4.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G28930.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5959ChloroplastSequence analysisAdd
BLAST
Chaini60 – 412353Protein kinase APK1B, chloroplasticPRO_0000024303Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei238 – 2381PhosphoserineBy similarity
Modified residuei239 – 2391PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP46573.
PRIDEiP46573.

PTM databases

iPTMnetiP46573.

Expressioni

Gene expression databases

ExpressionAtlasiP46573. baseline and differential.
GenevisibleiP46573. AT.

Interactioni

Protein-protein interaction databases

BioGridi2792. 1 interaction.
STRINGi3702.AT2G28930.1.

Structurei

3D structure databases

ProteinModelPortaliP46573.
SMRiP46573. Positions 30-352.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini69 – 356288Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1187. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000116550.
InParanoidiP46573.
PhylomeDBiP46573.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P46573-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGICLSAQIK AVSPGASPKY MSSEANDSLG SKSSSVSIRT NPRTEGEILQ
60 70 80 90 100
SPNLKSFTFA ELKAATRNFR PDSVLGEGGF GSVFKGWIDE QTLTASKPGT
110 120 130 140 150
GVVIAVKKLN QDGWQGHQEW LAEVNYLGQF SHPNLVKLIG YCLEDEHRLL
160 170 180 190 200
VYEFMPRGSL ENHLFRRGSY FQPLSWTLRL KVALGAAKGL AFLHNAETSV
210 220 230 240 250
IYRDFKTSNI LLDSEYNAKL SDFGLAKDGP TGDKSHVSTR IMGTYGYAAP
260 270 280 290 300
EYLATGHLTT KSDVYSYGVV LLEVLSGRRA VDKNRPPGEQ KLVEWARPLL
310 320 330 340 350
ANKRKLFRVI DNRLQDQYSM EEACKVATLA LRCLTFEIKL RPNMNEVVSH
360 370 380 390 400
LEHIQTLNEA GGRNIDMVQR RMRRRSDSVA INQKPNAGFA RQTAVGVIAT
410
AYPRPSDSPL FV
Length:412
Mass (Da):45,746
Last modified:December 1, 2000 - v2
Checksum:iEB1CA0B1A626A5DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005315 Genomic DNA. Translation: AAC33221.1.
AC005727 Genomic DNA. Translation: AAM15075.1.
CP002685 Genomic DNA. Translation: AEC08190.1.
D10152 mRNA. Translation: BAA20968.1.
PIRiT02725.
RefSeqiNP_001031439.1. NM_001036362.2. [P46573-1]
UniGeneiAt.46050.

Genome annotation databases

EnsemblPlantsiAT2G28930.2; AT2G28930.2; AT2G28930. [P46573-1]
GeneIDi817442.
KEGGiath:AT2G28930.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005315 Genomic DNA. Translation: AAC33221.1.
AC005727 Genomic DNA. Translation: AAM15075.1.
CP002685 Genomic DNA. Translation: AEC08190.1.
D10152 mRNA. Translation: BAA20968.1.
PIRiT02725.
RefSeqiNP_001031439.1. NM_001036362.2. [P46573-1]
UniGeneiAt.46050.

3D structure databases

ProteinModelPortaliP46573.
SMRiP46573. Positions 30-352.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi2792. 1 interaction.
STRINGi3702.AT2G28930.1.

PTM databases

iPTMnetiP46573.

Proteomic databases

PaxDbiP46573.
PRIDEiP46573.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G28930.2; AT2G28930.2; AT2G28930. [P46573-1]
GeneIDi817442.
KEGGiath:AT2G28930.

Organism-specific databases

TAIRiAT2G28930.

Phylogenomic databases

eggNOGiKOG1187. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000116550.
InParanoidiP46573.
PhylomeDBiP46573.

Enzyme and pathway databases

BioCyciARA:AT2G28930-MONOMER.
ARA:GQT-1640-MONOMER.
ARA:GQT-2470-MONOMER.
BRENDAi2.7.10.2. 399.

Miscellaneous databases

PROiP46573.

Gene expression databases

ExpressionAtlasiP46573. baseline and differential.
GenevisibleiP46573. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAPK1B_ARATH
AccessioniPrimary (citable) accession number: P46573
Secondary accession number(s): Q7GAG3, Q9SLH5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: December 1, 2000
Last modified: February 17, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.