Reviewed, UniProtKB/Swiss-Prot P46563 (ALF2_CAEEL)

Names and origin

Protein names

Recommended name:
    Fructose-bisphosphate aldolase 2
    EC=4.1.2.13
Alternative name(s):
    Aldolase CE-2
      Short name=CE2

Gene names

ORF Names:

F01F1.12

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Developmental stage	Restricted to the embryo and early larval stages.
Sequence similarities	Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase glycolysis Inferred from electronic annotation. Source: UniProtKB-KW locomotion Inferred from mutant phenotype. Source: WormBase oviposition Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase
Molecular function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: EC identical protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-314454,EBI-314454
	P91503	1	EBI-314454,EBI-318873
	Q9TZA7	1	EBI-314454,EBI-317106

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 366	366	Fructose-bisphosphate aldolase 2		PRO_0000216929
Sites
	Active site	191	1	Proton acceptor By similarity
	Active site	233	1	Schiff-base intermediate with dihydroxyacetone-P By similarity
	Binding site	60	1	Substrate By similarity
	Binding site	150	1	Substrate By similarity
	Site	366	1	Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate

Sequences

	Sequence		Length	Mass (Da)
	P46563-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: D6BD9D1469E69834	FASTA	366	38,846
	10 20 30 40 50 60 MATVGGAFKD SLTQAQKDEL HQIALKIVQD GKGILAADES TGTIGKRLDA INLENNETNR 70 80 90 100 110 120 QKYRQLLFTT PNLNQHISGV ILYEETFHQS TDKGEKFTDL LIKQGIVPGI KLDLGVVPLA 130 140 150 160 170 180 GTIGEGTTQG LDKLAERAAA FKKGGCGFAK WRCVLNIGTH TPSHLGMLEN ANVLARYASI 190 200 210 220 230 240 CQANGLVPIV EPEVLCDGEH DLARAQKVTE QVLAFVYKAL ADHHVYLEGT LLKPNMVTPG 250 260 270 280 290 300 QSSASKASHE AIGLATVTAL RRGVPAAVPG ITFLSGGQSE LDATANLNAI NSVQLGKPWK 310 320 330 340 350 360 LTFSYGRALQ ASVLKAWGGK DENIAAAQKT LLHRSKANGD ASLGKYAGED AAGAAAESLF VAKHSY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Caenorhabditis elegans has two isozymic forms, CE-1 and CE-2, of fructose-1,6-bisphosphate aldolase which are encoded by different genes." Inoue T., Yatsuki H., Kusakabe T., Joh K., Hori K. Arch. Biochem. Biophys. 339:226-234(1997) [PubMed: 9056253] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D83739 mRNA. Translation: BAA12092.1. U13070 Genomic DNA. Translation: AAC46646.1.
PIR	T15951.
RefSeq	NP_001021240.1.
UniGene	Cel.18124
3D structure databases
HSSP	HSSP built from PDB template 1FBA based on UniProtKB P07764.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:24398N.
IntAct	P46563. 8 interactions.
STRING	P46563.
Proteomic databases
PRIDE	P46563.
Genome annotation databases
Ensembl	F01F1.12a; F01F1.12a; F01F1.12; Caenorhabditis elegans. [Genome view]
GeneID	175827.
KEGG	cel:F01F1.12.
UCSC	F01F1.12a. c. elegans.
Organism-specific databases
CTD	175827.
WormBase	WBGene00017166. F01F1.12.
WormPep	F01F1.12. CE01225. [WorfDB]
Phylogenomic databases
OMA	ASICQAN.
Enzyme and pathway databases
BRENDA	4.1.2.13. 672.
Gene expression databases
ArrayExpress	P46563.
Family and domain databases
InterPro	IPR000741. Aldolase_I. IPR013785. Aldolase_TIM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11627. Aldolase_I. 1 hit.
Pfam	PF00274. Glycolytic. 1 hit. [Graphical view]
ProDom	PD001128. Aldolase_I. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00158. ALDOLASE_CLASS_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	889828.

Entry information

Entry name

ALF2_CAEEL

Accession

Primary (citable) accession number: P46563

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 3, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project