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Protein

Choline kinase B2

Gene

ckb-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the first step in phosphatidylcholine biosynthesis. Phosphorylates choline.1 Publication

Catalytic activityi

ATP + choline = ADP + phosphocholine.1 Publication

Cofactori

Mg2+1 PublicationNote: Less efficient with Mn2+.1 Publication

Kineticsi

  1. KM=13 mM for choline (at 37 degrees Celsius and pH 10)1 Publication
  2. KM=0.72 mM for ATP (at 37 degrees Celsius and pH 10)1 Publication
  1. Vmax=5.4 µmol/min/mg enzyme (at 37 degrees Celsius and pH 10)1 Publication

pH dependencei

Optimum pH is 10.1 Publication

Pathway:iphosphatidylcholine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes phosphocholine from choline.1 Publication
Proteins known to be involved in this subpathway in this organism are:
  1. Choline kinase A2 (cka-2), Choline kinase B2 (ckb-2)
This subpathway is part of the pathway phosphatidylcholine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphocholine from choline, the pathway phosphatidylcholine biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • choline kinase activity Source: WormBase

GO - Biological processi

  • CDP-choline pathway Source: WormBase
  • endoplasmic reticulum unfolded protein response Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_287639. Synthesis of PC.
REACT_293935. Synthesis of PE.
UniPathwayiUPA00753; UER00737.

Names & Taxonomyi

Protein namesi
Recommended name:
Choline kinase B21 Publication (EC:2.7.1.321 Publication)
Gene namesi
Name:ckb-2
ORF Names:B0285.9
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiB0285.9; CE33591; WBGene00000512; ckb-2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 369369Choline kinase B2PRO_0000206233Add
BLAST

Proteomic databases

PaxDbiP46559.
PRIDEiP46559.

Interactioni

Protein-protein interaction databases

BioGridi40801. 1 interaction.
STRINGi6239.B0285.9.

Structurei

3D structure databases

ProteinModelPortaliP46559.
SMRiP46559. Positions 29-356.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the choline/ethanolamine kinase family.Curated

Phylogenomic databases

eggNOGiCOG0510.
GeneTreeiENSGT00530000062991.
HOGENOMiHOG000015981.
InParanoidiP46559.
KOiK14156.
OMAiNIRIFCE.
OrthoDBiEOG7W41GK.
PhylomeDBiP46559.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.

Sequencei

Sequence statusi: Complete.

P46559-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAIEKFFTE KSPDSEQVLL KVIELGIDFL GGEWKNVDKS QVNVSRVHGG
60 70 80 90 100
QSNHMFHVTS STSATPYLLR IHRQPPSQVF TDTVNLAIFS ERGLGPKLYG
110 120 130 140 150
FFEGGRMEEF LPSKTFDVND VLVPENSRKI GAIFPLYHSI NVPVSKSRRC
160 170 180 190 200
VHLMREWLNG YESLGGGDYE ILPTTVNYSD HPKSVSIKDL NHEIDNFEKW
210 220 230 240 250
STEIFEHTLV FSHNDLASTN ILELNSTKEL VLIDWEFGTY NWRGFDLAMH
260 270 280 290 300
LSETAIDYRV PFPPGIKMNG DLIDNPPNIQ IFCEAYVEAD KKLKNRSPSD
310 320 330 340 350
PTAEVKALIQ ECQFFWPLTN LFWALSAMKH SLLKFENGVD LDVQARDRLA
360
VYFHLKPRSQ KIYEELSKK
Length:369
Mass (Da):42,264
Last modified:March 15, 2004 - v2
Checksum:i24384022ED1458B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY158691 mRNA. Translation: AAN41643.1.
Z34533 Genomic DNA. Translation: CAA84301.2.
PIRiT18696.
RefSeqiNP_497880.2. NM_065479.6.
UniGeneiCel.20657.

Genome annotation databases

EnsemblMetazoaiB0285.9; B0285.9; WBGene00000512.
GeneIDi175565.
KEGGicel:CELE_B0285.9.
UCSCiB0285.9. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY158691 mRNA. Translation: AAN41643.1.
Z34533 Genomic DNA. Translation: CAA84301.2.
PIRiT18696.
RefSeqiNP_497880.2. NM_065479.6.
UniGeneiCel.20657.

3D structure databases

ProteinModelPortaliP46559.
SMRiP46559. Positions 29-356.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi40801. 1 interaction.
STRINGi6239.B0285.9.

Proteomic databases

PaxDbiP46559.
PRIDEiP46559.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiB0285.9; B0285.9; WBGene00000512.
GeneIDi175565.
KEGGicel:CELE_B0285.9.
UCSCiB0285.9. c. elegans.

Organism-specific databases

CTDi175565.
WormBaseiB0285.9; CE33591; WBGene00000512; ckb-2.

Phylogenomic databases

eggNOGiCOG0510.
GeneTreeiENSGT00530000062991.
HOGENOMiHOG000015981.
InParanoidiP46559.
KOiK14156.
OMAiNIRIFCE.
OrthoDBiEOG7W41GK.
PhylomeDBiP46559.

Enzyme and pathway databases

UniPathwayiUPA00753; UER00737.
ReactomeiREACT_287639. Synthesis of PC.
REACT_293935. Synthesis of PE.

Miscellaneous databases

NextBioi888698.
PROiP46559.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Multiple isoforms of choline kinase from Caenorhabditis elegans: cloning, expression, purification, and characterization."
    Gee P., Kent C.
    Biochim. Biophys. Acta 1648:33-42(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, PATHWAY.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiCKB2_CAEEL
AccessioniPrimary (citable) accession number: P46559
Secondary accession number(s): Q8IS23
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: March 15, 2004
Last modified: April 29, 2015
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.