Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P46551

- CDK12_CAEEL

UniProt

P46551 - CDK12_CAEEL

Protein

Cyclin-dependent kinase 12

Gene

cdk-12

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 4 (06 Dec 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Cyclin-dependent kinase which displays CTD kinase activity: hyperphosphorylates 'Ser-2' in the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit, thereby acting as a key regulator of transcription elongation. Required for normal reproduction.1 Publication

    Catalytic activityi

    ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Phosphorylation at Thr-321 or Tyr-322 inactivates the enzyme.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei340 – 3401ATPPROSITE-ProRule annotation
    Active sitei444 – 4441Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi317 – 3259ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
    3. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    SignaLinkiC8JQQ9.
    C8JQR0.
    P46551.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase 12 (EC:2.7.11.22, EC:2.7.11.23)
    Alternative name(s):
    Cell division cycle 2-related protein kinase 7
    Cell division protein kinase 12
    Gene namesi
    Name:cdk-12
    Synonyms:cdtl-7
    ORF Names:B0285.1/B0285.2
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome III

    Organism-specific databases

    WormBaseiB0285.1a; CE31401; WBGene00007135; cdk-12.
    B0285.1b; CE44061; WBGene00007135; cdk-12.
    B0285.1c; CE44020; WBGene00007135; cdk-12.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Phosphorylation levels of 'Ser-2' of the RNA polymerase II C-terminal domain is undetectable in primordial germ cells and reduced by 60% in embryonic somatic nuclei. RNAi causes in sterility.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 730730Cyclin-dependent kinase 12PRO_0000085716Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei321 – 3211PhosphothreonineBy similarity
    Modified residuei322 – 3221PhosphotyrosineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP46551.

    Interactioni

    Protein-protein interaction databases

    BioGridi40795. 11 interactions.
    STRINGi6239.B0285.1.

    Structurei

    3D structure databases

    ProteinModelPortaliP46551.
    SMRiP46551. Positions 261-708.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini313 – 605293Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi12 – 121110Arg-richAdd
    BLAST
    Compositional biasi100 – 17071Ser-richAdd
    BLAST
    Compositional biasi189 – 27587Pro-richAdd
    BLAST
    Compositional biasi677 – 68711His-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00740000114964.
    HOGENOMiHOG000021386.
    InParanoidiP46551.
    KOiK08819.
    OMAiDSWYKTN.
    OrthoDBiEOG76DTSM.
    PhylomeDBiP46551.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform a (identifier: P46551-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEISPGSSTH ERDRKGSYGH RERTRSHSGS PSRFYSKDKR GSSRQGVRPR    50
    DRDSKDSISP QYKQRNWSRG GGGGGRDRGR NDFSYRKKGK DYNKRRDKRS 100
    RSRSRHRSPK RSGSSKKSKR RNSSGSSSSD LMDTSLMSEL KKHGDYGSSS 150
    KSKKKSRKRR KHSSSSSSSS GEAMDLPVSS NGMNVTAIPP PPSFNINPFQ 200
    PMFSQPPPPP LPPNSQFMTP PPRPPPAPFS IPPPSVDIHF AATASFSLSS 250
    IPPPPPQTDG GASSSKRQDP LPMPPDSKRI ATRPVITTRR GHATNRPSDS 300
    DSWYKTNLTH YTMLDQIGEG TYGQVYKAVN NLTGEQVALK RVRLENEKEG 350
    FPITAIREIK ILRQLHHKNI VRLMDIVIDD ISMDELKRTR ANFYLVFEYV 400
    DHDLIGLLES KELVDFNKDQ ICSLFKQLLE GLAYIHNTGF LHRDIKCSNI 450
    LVNNKGELKI ADLGLARLWE KESRLYTNRV ITLWYRPPEL LLGDERYGPA 500
    IDVWSTGCML GELFTRKPLF NGNNEFGQLE LISKVCGSPN VDNWPELTEL 550
    VGWNTFRMKR TYQRRIREEF EHIMPREAVD LLDKMLTLNP EKRISAKEAL 600
    NHPWIRSLEH TTVQPLKLPQ HQDCHEMWSK KQKKSARLGR QAEGSSGSGH 650
    SIRATSHPRA PTQPSTTTTK SNGSSNHHHH HHHSHHHASS LPPSGGHAPP 700
    PPPPPTQASS TSHNNHQPVP QSQYQSVFFK 730
    Length:730
    Mass (Da):82,429
    Last modified:December 6, 2002 - v4
    Checksum:iE347661352F39A0B
    GO
    Isoform c (identifier: P46551-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         660-660: A → AA

    Show »
    Length:731
    Mass (Da):82,500
    Checksum:i540325C857BB7735
    GO
    Isoform b (identifier: P46551-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         659-659: R → RVFEA

    Show »
    Length:734
    Mass (Da):82,875
    Checksum:i9D5EF81F17FDA31E
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei659 – 6591R → RVFEA in isoform b. CuratedVSP_056771
    Alternative sequencei660 – 6601A → AA in isoform c. CuratedVSP_056772

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z34533 Genomic DNA. Translation: CAA84302.3.
    Z34533 Genomic DNA. Translation: CBB15978.1.
    Z34533 Genomic DNA. Translation: CBB15981.1.
    PIRiT18689.
    T18697.
    RefSeqiNP_001254914.1. NM_001267985.1.
    NP_001254915.1. NM_001267986.1.
    NP_001254916.1. NM_001267987.1.
    UniGeneiCel.7463.

    Genome annotation databases

    EnsemblMetazoaiB0285.1a; B0285.1a; WBGene00007135.
    B0285.1b; B0285.1b; WBGene00007135.
    B0285.1c; B0285.1c; WBGene00007135.
    GeneIDi175559.
    KEGGicel:CELE_B0285.1.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z34533 Genomic DNA. Translation: CAA84302.3 .
    Z34533 Genomic DNA. Translation: CBB15978.1 .
    Z34533 Genomic DNA. Translation: CBB15981.1 .
    PIRi T18689.
    T18697.
    RefSeqi NP_001254914.1. NM_001267985.1.
    NP_001254915.1. NM_001267986.1.
    NP_001254916.1. NM_001267987.1.
    UniGenei Cel.7463.

    3D structure databases

    ProteinModelPortali P46551.
    SMRi P46551. Positions 261-708.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 40795. 11 interactions.
    STRINGi 6239.B0285.1.

    Proteomic databases

    PaxDbi P46551.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai B0285.1a ; B0285.1a ; WBGene00007135 .
    B0285.1b ; B0285.1b ; WBGene00007135 .
    B0285.1c ; B0285.1c ; WBGene00007135 .
    GeneIDi 175559.
    KEGGi cel:CELE_B0285.1.

    Organism-specific databases

    CTDi 175559.
    WormBasei B0285.1a ; CE31401 ; WBGene00007135 ; cdk-12.
    B0285.1b ; CE44061 ; WBGene00007135 ; cdk-12.
    B0285.1c ; CE44020 ; WBGene00007135 ; cdk-12.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00740000114964.
    HOGENOMi HOG000021386.
    InParanoidi P46551.
    KOi K08819.
    OMAi DSWYKTN.
    OrthoDBi EOG76DTSM.
    PhylomeDBi P46551.

    Enzyme and pathway databases

    SignaLinki C8JQQ9.
    C8JQR0.
    P46551.

    Miscellaneous databases

    NextBioi 888674.
    PROi P46551.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.
    2. "Phosphorylation of RNA polymerase II is independent of P-TEFb in the C. elegans germline."
      Bowman E.A., Bowman C.R., Ahn J.H., Kelly W.G.
      Development 140:3703-3713(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiCDK12_CAEEL
    AccessioniPrimary (citable) accession number: P46551
    Secondary accession number(s): C8JQQ9, C8JQR0, P46552
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: December 6, 2002
    Last modified: October 1, 2014
    This is version 120 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3