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Protein

Cyclin-dependent kinase 12

Gene

cdk-12

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cyclin-dependent kinase which displays CTD kinase activity: hyperphosphorylates 'Ser-2' in the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit, thereby acting as a key regulator of transcription elongation. Required for normal reproduction.1 Publication

Catalytic activityi

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.1 Publication
ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei340 – 3401ATPPROSITE-ProRule annotationBy similarity
Active sitei444 – 4441Proton acceptorPROSITE-ProRule annotationBy similarity
Binding sitei625 – 6251ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi317 – 3259ATPPROSITE-ProRule annotationBy similarity
Nucleotide bindingi398 – 4036ATPBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiC8JQQ9.
C8JQR0.
P46551.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 12 (EC:2.7.11.22, EC:2.7.11.23)
Alternative name(s):
Cell division cycle 2-related protein kinase 7
Cell division protein kinase 12
Gene namesi
Name:cdk-12
Synonyms:cdtl-7
ORF Names:B0285.1/B0285.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiB0285.1a; CE31401; WBGene00007135; cdk-12.
B0285.1b; CE44061; WBGene00007135; cdk-12.
B0285.1c; CE44020; WBGene00007135; cdk-12.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

  • chromosome Source: WormBase
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Phosphorylation of 'Ser-2' of the RNA polymerase II C-terminal domain is undetectable in primordial germ cells and reduced by 60% in embryonic somatic nuclei. RNAi treatment causes sterility.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 730730Cyclin-dependent kinase 12PRO_0000085716Add
BLAST

Proteomic databases

PaxDbiP46551.

Interactioni

Protein-protein interaction databases

BioGridi40795. 11 interactions.
STRINGi6239.B0285.1b.

Structurei

3D structure databases

ProteinModelPortaliP46551.
SMRiP46551. Positions 280-708.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini313 – 605293Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi12 – 121110Arg-richAdd
BLAST
Compositional biasi100 – 17071Ser-richAdd
BLAST
Compositional biasi189 – 27587Pro-richAdd
BLAST
Compositional biasi677 – 68711His-richAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00800000124088.
HOGENOMiHOG000021386.
InParanoidiP46551.
KOiK08819.
OMAiYKTNLTH.
OrthoDBiEOG76DTSM.
PhylomeDBiP46551.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: P46551-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEISPGSSTH ERDRKGSYGH RERTRSHSGS PSRFYSKDKR GSSRQGVRPR
60 70 80 90 100
DRDSKDSISP QYKQRNWSRG GGGGGRDRGR NDFSYRKKGK DYNKRRDKRS
110 120 130 140 150
RSRSRHRSPK RSGSSKKSKR RNSSGSSSSD LMDTSLMSEL KKHGDYGSSS
160 170 180 190 200
KSKKKSRKRR KHSSSSSSSS GEAMDLPVSS NGMNVTAIPP PPSFNINPFQ
210 220 230 240 250
PMFSQPPPPP LPPNSQFMTP PPRPPPAPFS IPPPSVDIHF AATASFSLSS
260 270 280 290 300
IPPPPPQTDG GASSSKRQDP LPMPPDSKRI ATRPVITTRR GHATNRPSDS
310 320 330 340 350
DSWYKTNLTH YTMLDQIGEG TYGQVYKAVN NLTGEQVALK RVRLENEKEG
360 370 380 390 400
FPITAIREIK ILRQLHHKNI VRLMDIVIDD ISMDELKRTR ANFYLVFEYV
410 420 430 440 450
DHDLIGLLES KELVDFNKDQ ICSLFKQLLE GLAYIHNTGF LHRDIKCSNI
460 470 480 490 500
LVNNKGELKI ADLGLARLWE KESRLYTNRV ITLWYRPPEL LLGDERYGPA
510 520 530 540 550
IDVWSTGCML GELFTRKPLF NGNNEFGQLE LISKVCGSPN VDNWPELTEL
560 570 580 590 600
VGWNTFRMKR TYQRRIREEF EHIMPREAVD LLDKMLTLNP EKRISAKEAL
610 620 630 640 650
NHPWIRSLEH TTVQPLKLPQ HQDCHEMWSK KQKKSARLGR QAEGSSGSGH
660 670 680 690 700
SIRATSHPRA PTQPSTTTTK SNGSSNHHHH HHHSHHHASS LPPSGGHAPP
710 720 730
PPPPPTQASS TSHNNHQPVP QSQYQSVFFK
Length:730
Mass (Da):82,429
Last modified:December 6, 2002 - v4
Checksum:iE347661352F39A0B
GO
Isoform c (identifier: P46551-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     660-660: A → AA

Show »
Length:731
Mass (Da):82,500
Checksum:i540325C857BB7735
GO
Isoform b (identifier: P46551-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     659-659: R → RVFEA

Show »
Length:734
Mass (Da):82,875
Checksum:i9D5EF81F17FDA31E
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei659 – 6591R → RVFEA in isoform b. CuratedVSP_056771
Alternative sequencei660 – 6601A → AA in isoform c. CuratedVSP_056772

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z34533 Genomic DNA. Translation: CAA84302.3.
Z34533 Genomic DNA. Translation: CBB15978.1.
Z34533 Genomic DNA. Translation: CBB15981.1.
PIRiT18689.
T18697.
RefSeqiNP_001254914.1. NM_001267985.1. [P46551-3]
NP_001254915.1. NM_001267986.1. [P46551-2]
NP_001254916.1. NM_001267987.1. [P46551-1]
UniGeneiCel.7463.

Genome annotation databases

EnsemblMetazoaiB0285.1a; B0285.1a; WBGene00007135. [P46551-1]
GeneIDi175559.
KEGGicel:CELE_B0285.1.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z34533 Genomic DNA. Translation: CAA84302.3.
Z34533 Genomic DNA. Translation: CBB15978.1.
Z34533 Genomic DNA. Translation: CBB15981.1.
PIRiT18689.
T18697.
RefSeqiNP_001254914.1. NM_001267985.1. [P46551-3]
NP_001254915.1. NM_001267986.1. [P46551-2]
NP_001254916.1. NM_001267987.1. [P46551-1]
UniGeneiCel.7463.

3D structure databases

ProteinModelPortaliP46551.
SMRiP46551. Positions 280-708.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi40795. 11 interactions.
STRINGi6239.B0285.1b.

Proteomic databases

PaxDbiP46551.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiB0285.1a; B0285.1a; WBGene00007135. [P46551-1]
GeneIDi175559.
KEGGicel:CELE_B0285.1.

Organism-specific databases

CTDi175559.
WormBaseiB0285.1a; CE31401; WBGene00007135; cdk-12.
B0285.1b; CE44061; WBGene00007135; cdk-12.
B0285.1c; CE44020; WBGene00007135; cdk-12.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00800000124088.
HOGENOMiHOG000021386.
InParanoidiP46551.
KOiK08819.
OMAiYKTNLTH.
OrthoDBiEOG76DTSM.
PhylomeDBiP46551.

Enzyme and pathway databases

SignaLinkiC8JQQ9.
C8JQR0.
P46551.

Miscellaneous databases

NextBioi888674.
PROiP46551.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "Phosphorylation of RNA polymerase II is independent of P-TEFb in the C. elegans germline."
    Bowman E.A., Bowman C.R., Ahn J.H., Kelly W.G.
    Development 140:3703-3713(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiCDK12_CAEEL
AccessioniPrimary (citable) accession number: P46551
Secondary accession number(s): C8JQQ9, C8JQR0, P46552
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: December 6, 2002
Last modified: July 22, 2015
This is version 127 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.