P46551 (CDK12_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase 12
EC=2.7.11.22
EC=2.7.11.23

Alternative name(s):
Cell division cycle 2-related protein kinase 7
Cell division protein kinase 12

Gene names

Name:	cdtl-7
ORF Names:	B0285.1/B0285.2

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	730 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cyclin-dependent kinase which displays CTD kinase activity: hyperphosphorylates the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit, thereby acting as a key regulator of transcription elongation By similarity.
Catalytic activity	ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate. ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Phosphorylation at Thr-321 or Tyr-322 inactivates the enzyme By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: GOC
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 730	730	Cyclin-dependent kinase 12		PRO_0000085716
Regions
	Domain	313 – 605	293	Protein kinase
	Nucleotide binding	317 – 325	9	ATP By similarity
	Compositional bias	12 – 121	110	Arg-rich
	Compositional bias	100 – 170	71	Ser-rich
	Compositional bias	189 – 275	87	Pro-rich
	Compositional bias	677 – 687	11	His-rich
Sites
	Active site	444	1	Proton acceptor By similarity
	Binding site	340	1	ATP By similarity
Amino acid modifications
	Modified residue	321	1	Phosphothreonine By similarity
	Modified residue	322	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P46551 [UniParc]. Last modified December 6, 2002. Version 4. Checksum: E347661352F39A0B	FASTA	730	82,429
	10 20 30 40 50 60 MEISPGSSTH ERDRKGSYGH RERTRSHSGS PSRFYSKDKR GSSRQGVRPR DRDSKDSISP 70 80 90 100 110 120 QYKQRNWSRG GGGGGRDRGR NDFSYRKKGK DYNKRRDKRS RSRSRHRSPK RSGSSKKSKR 130 140 150 160 170 180 RNSSGSSSSD LMDTSLMSEL KKHGDYGSSS KSKKKSRKRR KHSSSSSSSS GEAMDLPVSS 190 200 210 220 230 240 NGMNVTAIPP PPSFNINPFQ PMFSQPPPPP LPPNSQFMTP PPRPPPAPFS IPPPSVDIHF 250 260 270 280 290 300 AATASFSLSS IPPPPPQTDG GASSSKRQDP LPMPPDSKRI ATRPVITTRR GHATNRPSDS 310 320 330 340 350 360 DSWYKTNLTH YTMLDQIGEG TYGQVYKAVN NLTGEQVALK RVRLENEKEG FPITAIREIK 370 380 390 400 410 420 ILRQLHHKNI VRLMDIVIDD ISMDELKRTR ANFYLVFEYV DHDLIGLLES KELVDFNKDQ 430 440 450 460 470 480 ICSLFKQLLE GLAYIHNTGF LHRDIKCSNI LVNNKGELKI ADLGLARLWE KESRLYTNRV 490 500 510 520 530 540 ITLWYRPPEL LLGDERYGPA IDVWSTGCML GELFTRKPLF NGNNEFGQLE LISKVCGSPN 550 560 570 580 590 600 VDNWPELTEL VGWNTFRMKR TYQRRIREEF EHIMPREAVD LLDKMLTLNP EKRISAKEAL 610 620 630 640 650 660 NHPWIRSLEH TTVQPLKLPQ HQDCHEMWSK KQKKSARLGR QAEGSSGSGH SIRATSHPRA 670 680 690 700 710 720 PTQPSTTTTK SNGSSNHHHH HHHSHHHASS LPPSGGHAPP PPPPPTQASS TSHNNHQPVP 730 QSQYQSVFFK

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z34533 Genomic DNA. Translation: CAA84302.3.
PIR	T18689. T18697.
RefSeq	NP_001254916.1. NM_001267987.1.
3D structure databases
ProteinModelPortal	P46551.
SMR	P46551. Positions 280-708.
ModBase	Search...
Protein-protein interaction databases
STRING	6239.B0285.1.
Proteomic databases
PaxDb	P46551.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	B0285.1a; B0285.1a; B0285.1.
GeneID	175559.
KEGG	cel:CELE_B0285.1.
Organism-specific databases
CTD	175559.
WormBase	B0285.1a; CE31401; WBGene00007135; cdtl-7.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00690000102025.
HOGENOM	HOG000021386.
InParanoid	P46551.
Gene expression databases
ArrayExpress	P46551.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	888674.

Entry information

Entry name

CDK12_CAEEL

Accession

Primary (citable) accession number: P46551
Secondary accession number(s): P46552

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	December 6, 2002
Last modified:	May 1, 2013
This is version 110 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project