P46547 (PIP_AERSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline iminopeptidase
Short name=PIP
EC=3.4.11.5

Alternative name(s):
Prolyl aminopeptidase
Short name=PAP

Gene names

Name:	pip
Synonyms:	pap

Organism

Aeromonas sobria

Taxonomic identifier

646 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Higher activity toward long peptides. Acts on hydroxyproline beta-naphthylamide with almost as high an activity as on proline beta-naphthylamide.
Catalytic activity	Release of N-terminal proline from a peptide.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the peptidase S33 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 425	424	Proline iminopeptidase		PRO_0000080834
Sites
	Active site	146	1	Nucleophile By similarity
	Active site	351	1	By similarity
	Active site	404	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P46547 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 774F14CC3F797F28	FASTA	425	48,406
	10 20 30 40 50 60 MSSPLHYVLD GIHCEPHFFT VPLDHQQPDD EETITLFGRT LCRKDRLDDE LPWLLYLQGG 70 80 90 100 110 120 PGFGAPRPSA NGGWIKRALQ EFRVLLLDQR GTGHSTPIHA ELLAHLNPRQ QADYLSHFRA 130 140 150 160 170 180 DSIVRDAELI REQLSPDHPW SLLGQSFGGF CSLTYLSLFP DSLHEVYLTG GVAPIGRSAD 190 200 210 220 230 240 EVYRATYQRV ADKNRAFFAR FPHAQAIANR LATHLQRHDV RLPNGQRLTV EQLQQQGLDL 250 260 270 280 290 300 GASGAFEELY YLLEDAFIGE KLNPAFLYQV QAMQPFNTNP VFAILHELIY CEGAASHWAA 310 320 330 340 350 360 ERVRGEFPAL AWAQGKDFAF TGEMIFPWMF EQFRELIPLK EAAHLLAEKA DWGPLYDPVQ 370 380 390 400 410 420 LARNKVPVAC AVYAEDMYVE FDYSRETLKG LSNSRAWITN EYEHNGLRVD GEQILDRLIR LNRDC

References

[1]

"Isolation and characterization of the prolyl aminopeptidase gene (pap) from Aeromonas sobria: comparison with the Bacillus coagulans enzyme." Kitazono A., Kitano A., Tsuru D., Yoshimoto T. J. Biochem. 116:818-825(1994) [PubMed: 7883756] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D30714 Genomic DNA. Translation: BAA06380.1.
PIR	JC4184.
3D structure databases
ProteinModelPortal	P46547.
ModBase	Search...
Protein family/group databases
MEROPS	S33.008.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000073. AB_hydrolase_1. IPR002410. Peptidase_S33. [Graphical view]
Pfam	PF00561. Abhydrolase_1. 1 hit. [Graphical view]
PRINTS	PR00793. PROAMNOPTASE.
ProtoNet	Search...

Entry information

Entry name

PIP_AERSO

Accession

Primary (citable) accession number: P46547

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 62 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program