P46542 (PIP_LACDL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline iminopeptidase
Short name=PIP
EC=3.4.11.5

Alternative name(s):
Prolyl aminopeptidase
Short name=PAP

Gene names

Name:	pip
Synonyms:	pepI

Organism

Lactobacillus delbrueckii subsp. lactis

Taxonomic identifier

29397 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Releases the N-terminal proline from various substrates. Cleaves Pro-betaNA (L-prolyl-beta-naphthylamide) effectively. Ref.1
Catalytic activity	Release of N-terminal proline from a peptide. Ref.1
Enzyme regulation	Inhibited by 3,4-DCI, but no significant effect on enzyme activity by pepstatin A, E-64, 1,10-phenanthroline or EDTA. Ref.1
Subunit structure	Homotrimer By similarity.
Subcellular location	Cell envelope Potential.
Sequence similarities	Belongs to the peptidase S33 family.

Ontologies

Keywords
Molecular function	Aminopeptidase Hydrolase Protease
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro envelope Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	Proline iminopeptidase		PRO_0000080837
Sites
	Active site	106	1	Nucleophile By similarity
	Active site	245	1	By similarity
	Active site	272	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P46542 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: A5E369D34F1620F3	FASTA	294	32,879
	10 20 30 40 50 60 MQITEKYLPF GNWQTYCRIV GEATDRAPLL LLHGGPGSSH NYFEVLDQVA EKSGRQVIMY 70 80 90 100 110 120 DQLGCGNSSI PDDQAETAYT AQTWVKELEN VREQLGLDQI HLLGQSWGGM LALIYLCDYQ 130 140 150 160 170 180 PKGVKSLILS STLASAKLWS QELHRLIKYL PKGEQAAIKE AETTGNYDSP AYQAANAHFM 190 200 210 220 230 240 DQHAINVTPD LPEPVLRKKK GGNLAYLTGW GPNEYTPIGN LHGYEYTDRL KDLDLPALIT 250 260 270 280 290 SGTDDLCTPL VAKSMYDHLP NARWELFAGC GHMPFVQENA KYQELLSDWL ISQD

References

[1]	"Cloning, heterologous expression, and sequencing of a novel proline iminopeptidase gene, pepI, from Lactobacillus delbrueckii subsp. lactis DSM 7290." Klein J.R., Schmidt U., Plapp R. Microbiology 140:1133-1139(1994) [PubMed: 8025678] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION. Strain: DSM 7290.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z26948 Genomic DNA. Translation: CAA81556.1.
PIR	A59087.
3D structure databases
ProteinModelPortal	P46542.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR005945. Pept_S33_TRI_F1. IPR002410. Peptidase_S33. [Graphical view]
PIRSF	PIRSF005539. Pept_S33_TRI_F1. 1 hit.
PRINTS	PR00793. PROAMNOPTASE.
TIGRFAMs	TIGR01250. Pro_imino_pep_2. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PIP_LACDL

Accession

Primary (citable) accession number: P46542

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 16, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program