P46534 (PYRE_BACCL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Orotate phosphoribosyltransferase
Short name=OPRT
Short name=OPRTase
EC=2.4.2.10

Gene names

Name:

pyrE

Organism

Bacillus caldolyticus

Taxonomic identifier

1394 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	206 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Induction	Repressed by uracil. Ref.1
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 206	206	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_0000110666
Regions
	Region	119 – 127	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	93	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	97	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	99	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	123	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P46534 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 72FDD9B0419335E0	FASTA	206	22,402
	10 20 30 40 50 60 MKHDIAAKLL QIGAVALQPN EPFTWSSGLK SPIYCDNRLT LAYPGVRRLI ADALAELIRT 70 80 90 100 110 120 HFPKADLIAG TAAGIPHAAW VSERLELPMC YVRSQAKRHG KGKQIEGQAR PGQRVVVIED 130 140 150 160 170 180 LISTGGTSLA AVRALKEAGC EVLGVAAIFT YGLEKAKQAF AAENLPAYTL TDYNTLIETA 190 200 VRLGAVSEHD LATLRQWREN PEEWGS

References

[1]	"Molecular characterization of pyrimidine biosynthesis genes from the thermophile Bacillus caldolyticus." Ghim S.Y., Nielsen P., Neuhard J. Microbiology 140:479-491(1994) [PubMed: 7516791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION. Strain: DSM 405 / NBRC 15313 / YP-T.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X73308 Genomic DNA. Translation: CAA51743.1.
PIR	I40173.
3D structure databases
ProteinModelPortal	P46534.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_01208. PyrE. [Tree]
InterPro	IPR004467. Or_phspho_trans_clade-1. IPR023031. Orotate_PribosylTferase. IPR000836. PRibTrfase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR00336. PyrE. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_BACCL

Accession

Primary (citable) accession number: P46534

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	September 21, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program