ID CDNB_HUMAN STANDARD; PRT; 198 AA. AC P46527; Q16307; Q9BUS6; DT 01-NOV-1995 (Rel. 32, Created) DT 01-NOV-1995 (Rel. 32, Last sequence update) DT 15-MAR-2004 (Rel. 43, Last annotation update) DE Cyclin-dependent kinase inhibitor 1B (Cyclin-dependent kinase DE inhibitor p27) (p27Kip1). GN CDKN1B OR KIP1. OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP SEQUENCE FROM N.A., AND SEQUENCE OF 28-79 AND 104-152. RC TISSUE=Kidney; RX MEDLINE=94306518; PubMed=8033212; RA Polyak K., Lee M.-H., Erdjument-Bromage H., Koff A., Roberts J.M., RA Tempst P., Massague J.; RT "Cloning of p27Kip1, a cyclin-dependent kinase inhibitor and a RT potential mediator of extracellular antimitogenic signals."; RL Cell 78:59-66(1994). RN [2] RP SEQUENCE FROM N.A. RX MEDLINE=95188144; PubMed=7882309; RA Pietenpol J.A., Bohlander S.K., Sato Y., Papadopoulos N., Liu B., RA Friedman C., Trask B.J., Roberts J.M., Kinzler K.W., Rowley J.D.; RT "Assignment of the human p27Kip1 gene to 12p13 and its analysis in RT leukemias."; RL Cancer Res. 55:1206-1210(1995). RN [3] RP SEQUENCE FROM N.A., AND VARIANTS TRP-15 AND GLY-109. RA Rieder M.J., Braun A.C., Montoya M.A., Chung M.-W., Nguyen C.P., RA Nguyen D.A., Livingston R.J., Poel C.L., Robertson P.D., RA Schackwitz W.S., Sherwood J.K., Witrak L.A., Nickerson D.A.; RL Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases. RN [4] RP SEQUENCE FROM N.A., AND VARIANT GLY-109. RC TISSUE=Cervix; RX MEDLINE=22388257; PubMed=12477932; RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., RA Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., RA Altschul S.F., Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., RA Hopkins R.F., Jordan H., Moore T., Max S.I., Wang J., Hsieh F., RA Diatchenko L., Marusina K., Farmer A.A., Rubin G.M., Hong L., RA Stapleton M., Soares M.B., Bonaldo M.F., Casavant T.L., Scheetz T.E., RA Brownstein M.J., Usdin T.B., Toshiyuki S., Carninci P., Prange C., RA Raha S.S., Loquellano N.A., Peters G.J., Abramson R.D., Mullahy S.J., RA Bosak S.A., McEwan P.J., McKernan K.J., Malek J.A., Gunaratne P.H., RA Richards S., Worley K.C., Hale S., Garcia A.M., Gay L.J., Hulyk S.W., RA Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., Gibbs R.A., RA Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., RA Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G., RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., RA Rodriguez A.C., Grimwood J., Schmutz J., Myers R.M., RA Butterfield Y.S.N., Krzywinski M.I., Skalska U., Smailus D.E., RA Schnerch A., Schein J.E., Jones S.J.M., Marra M.A.; RT "Generation and initial analysis of more than 15,000 full-length RT human and mouse cDNA sequences."; RL Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002). RN [5] RP X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 23-106 OF COMPLEX WITH CDK2 RP AND CG2A. RX MEDLINE=96300318; PubMed=8684460; RA Russo A.A., Jeffrey P.D., Patten A.K., Massague J., Pavletich N.P.; RT "Crystal structure of the p27Kip1 cyclin-dependent-kinase inhibitor RT bound to the cyclin A-Cdk2 complex."; RL Nature 382:325-331(1996). CC -!- FUNCTION: Involved in G1 arrest. May mediate TGF beta-induced G1 CC arrest. Binds to and inhibits complexes formed by cyclin E-CDK2, CC cyclin A-CDK2, and cyclin D1-CDK4. Interaction with nucleoporin CC NUP50 is required for nuclear import and for degradation of CC phosphorylated p27Kip1 after nuclear import (By similarity). CC -!- SUBUNIT: Interacts with NUP50 (By similarity). CC -!- SUBCELLULAR LOCATION: Nuclear. CC -!- TISSUE SPECIFICITY: Expressed in all tissues tested. Highest CC levels in skeletal muscle, lowest in liver and kidney. CC -!- DOMAIN: A peptide sequence containing only AA 28-79 retains CC substantial Kip1 cyclin A/CDK2 inhibitory activity. CC -!- SIMILARITY: Belongs to the CDI family. CC -!- DATABASE: NAME=Atlas Genet. Cytogenet. Oncol. Haematol.; CC WWW="http://www.infobiogen.fr/services/chromcancer/Genes/CDKN1BID116.html". CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U10906; AAA20240.1; -. DR EMBL; S76988; AAD14244.1; -. DR EMBL; S76986; AAD14244.1; JOINED. DR EMBL; AF480891; AAL78041.1; -. DR EMBL; BC001971; AAH01971.1; -. DR PDB; 1JSU; 29-JUL-97. DR SWISS-2DPAGE; P46527; HUMAN. DR Genew; HGNC:1785; CDKN1B. DR MIM; 600778; -. DR GO; GO:0004861; F:cyclin-dependent protein kinase inhibitor a...; TAS. DR GO; GO:0005072; F:TGFbeta receptor, cytoplasmic mediator acti...; TAS. DR GO; GO:0007050; P:cell cycle arrest; TAS. DR GO; GO:0008285; P:negative regulation of cell proliferation; TAS. DR GO; GO:0000079; P:regulation of CDK activity; TAS. DR InterPro; IPR003175; CDI. DR Pfam; PF02234; CDI; 1. KW Protein kinase inhibitor; Cell cycle; Nuclear protein; 3D-structure; KW Polymorphism. FT DOMAIN 153 169 NUCLEAR LOCALIZATION SIGNAL (POTENTIAL). FT VARIANT 15 15 R -> W (in dbSNP:2066828). FT /FTId=VAR_011871. FT VARIANT 109 109 V -> G (in dbSNP:2066827). FT /FTId=VAR_011872. FT CONFLICT 22 22 E -> D (in Ref. 2). FT TURN 27 28 FT HELIX 38 49 FT TURN 50 53 FT HELIX 54 60 FT TURN 61 61 FT STRAND 62 63 FT TURN 64 67 FT STRAND 68 69 FT STRAND 77 80 FT TURN 81 82 FT HELIX 86 89 SQ SEQUENCE 198 AA; 22073 MW; 1118D58901CDF3FC CRC64; MSNVRVSNGS PSLERMDARQ AEHPKPSACR NLFGPVDHEE LTRDLEKHCR DMEEASQRKW NFDFQNHKPL EGKYEWQEVE KGSLPEFYYR PPRPPKGACK VPAQESQDVS GSRPAAPLIG APANSEDTHL VDPKTDPSDS QTGLAEQCAG IRKRPATDDS STQNKRANRT EENVSDGSPN AGSVEQTPKK PGLRRRQT //