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P46493

- ILVA_HAEIN

UniProt

P46493 - ILVA_HAEIN

Protein

L-threonine dehydratase biosynthetic IlvA

Gene

ilvA

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2-ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA By similarity.By similarity

    Catalytic activityi

    L-threonine = 2-oxobutanoate + NH3.

    Cofactori

    Pyridoxal phosphate.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei90 – 901Pyridoxal phosphateBy similarity
    Binding sitei316 – 3161Pyridoxal phosphateBy similarity

    GO - Molecular functioni

    1. L-threonine ammonia-lyase activity Source: UniProtKB-EC
    2. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. isoleucine biosynthetic process Source: UniProtKB-UniPathway
    2. threonine metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Isoleucine biosynthesis

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    UniPathwayiUPA00047; UER00054.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-threonine dehydratase biosynthetic IlvA (EC:4.3.1.19)
    Alternative name(s):
    Threonine deaminase
    Gene namesi
    Name:ilvA
    Ordered Locus Names:HI_0738.1
    OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
    Taxonomic identifieri71421 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000000579: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 513513L-threonine dehydratase biosynthetic IlvAPRO_0000185574Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei63 – 631N6-(pyridoxal phosphate)lysineBy similarity

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi71421.HI0738.1.

    Structurei

    3D structure databases

    ProteinModelPortaliP46493.
    SMRiP46493. Positions 10-512.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini340 – 41172ACT-like 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini433 – 50472ACT-like 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni190 – 1934Pyridoxal phosphate bindingBy similarity

    Sequence similaritiesi

    Contains 2 ACT-like domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1171.
    KOiK01754.
    OMAiGMKKYIQ.
    OrthoDBiEOG6ZSP7D.
    PhylomeDBiP46493.

    Family and domain databases

    InterProiIPR001721. ACT-like_dom.
    IPR000634. Ser/Thr_deHydtase_PyrdxlP-BS.
    IPR005787. Thr_deHydtase_biosynth.
    IPR001926. Trp_syn_b_sub_like_PLP_eny_SF.
    [Graphical view]
    PfamiPF00291. PALP. 1 hit.
    PF00585. Thr_dehydrat_C. 2 hits.
    [Graphical view]
    SUPFAMiSSF53686. SSF53686. 1 hit.
    TIGRFAMsiTIGR01124. ilvA_2Cterm. 1 hit.
    PROSITEiPS51672. ACT_LIKE. 2 hits.
    PS00165. DEHYDRATASE_SER_THR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P46493-1 [UniParc]FASTAAdd to Basket

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    MKNLLTNPQP SQSDYINAIV KLGSRVYEAA QVTPLQKMGK LSERLHNNIW    50
    IKREDRQPVN SFKLRGAYAM ISSLSAEQKA AGVIAASAGN HAQGVALSAK 100
    QLGLKALIVM PQNTPSIKVD AVRGFGGEVL LHGANFDEAK AKAIELSKEK 150
    NMTFIPPFDH PLVIAGQGTL AMEMLQQVAD LDYVFVQVGG GGLAAGVAIL 200
    LKQFMPEIKI IGVESKDSAC LKAALDKGEP TDLTHIGLFA DGVAVKRIGD 250
    ETFRLCQQYL DDMVLVDSDE VCAAMKDLFE NVRAVAEPSG ALGLAGLKKY 300
    VKQNHIEGKN MAAILSGANL NFHTLRYVSE RCEIGENREA LLAVTMPEQP 350
    GSFLKFAYVL GNRAVTEFSY RYADDKRACV FVGVRTTNEQ EKADIIADLT 400
    KNGFDVEDMS DDDIAKTHVR YLMGGRAAND NERLYTFEFP EQKGALLKFL 450
    ETLQNRWNIS LFHYRAHGAD YGNILAGFQI EQREQAEFEQ GLAQLNYVFE 500
    DVTKSKSYRY FLR 513
    Length:513
    Mass (Da):56,663
    Last modified:November 1, 1995 - v1
    Checksum:iDF42CA8B6FDE4CD7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC22398.1.
    RefSeqiNP_438898.1. NC_000907.1.
    WP_010869046.1. NC_000907.1.

    Genome annotation databases

    EnsemblBacteriaiAAC22398; AAC22398; HI_0738.1.
    GeneIDi950764.
    KEGGihin:HI0738.1.
    PATRICi20190119. VBIHaeInf48452_0775.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC22398.1 .
    RefSeqi NP_438898.1. NC_000907.1.
    WP_010869046.1. NC_000907.1.

    3D structure databases

    ProteinModelPortali P46493.
    SMRi P46493. Positions 10-512.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 71421.HI0738.1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC22398 ; AAC22398 ; HI_0738.1 .
    GeneIDi 950764.
    KEGGi hin:HI0738.1.
    PATRICi 20190119. VBIHaeInf48452_0775.

    Phylogenomic databases

    eggNOGi COG1171.
    KOi K01754.
    OMAi GMKKYIQ.
    OrthoDBi EOG6ZSP7D.
    PhylomeDBi P46493.

    Enzyme and pathway databases

    UniPathwayi UPA00047 ; UER00054 .

    Family and domain databases

    InterProi IPR001721. ACT-like_dom.
    IPR000634. Ser/Thr_deHydtase_PyrdxlP-BS.
    IPR005787. Thr_deHydtase_biosynth.
    IPR001926. Trp_syn_b_sub_like_PLP_eny_SF.
    [Graphical view ]
    Pfami PF00291. PALP. 1 hit.
    PF00585. Thr_dehydrat_C. 2 hits.
    [Graphical view ]
    SUPFAMi SSF53686. SSF53686. 1 hit.
    TIGRFAMsi TIGR01124. ilvA_2Cterm. 1 hit.
    PROSITEi PS51672. ACT_LIKE. 2 hits.
    PS00165. DEHYDRATASE_SER_THR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
    2. Koonin E.V., Rudd K.E.
      Submitted (SEP-1995) to UniProtKB
      Cited for: IDENTIFICATION.

    Entry informationi

    Entry nameiILVA_HAEIN
    AccessioniPrimary (citable) accession number: P46493
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Haemophilus influenzae
      Haemophilus influenzae (strain Rd): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3