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P46489 (MDHP_FLABI) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase [NADP], chloroplastic

EC=1.1.1.82
Alternative name(s):
NADP-MDH
OrganismFlaveria bidentis (Coastal plain yellowtops) (Ethulia bidentis)
Taxonomic identifier4224 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsAsteralesAsteraceaeAsteroideaeHeliantheae allianceTageteaeFlaveria

Protein attributes

Sequence length453 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The chloroplastic, NADP-dependent form is essential for the photosynthesis C4 cycle, which allows plants to circumvent the problem of photorespiration. In C4 plants, NADP-MDH activity acts to convert oxaloacetate to malate in chloroplasts of mesophyll cells for transport to the bundle sheath cells.

Catalytic activity

(S)-malate + NADP+ = oxaloacetate + NADPH.

Enzyme regulation

Chloroplast NADP-MDH is activated upon illumination. In order to be enzymatically active, disulfide bridges on the protein must be reduced by thioredoxin which receives electrons from ferredoxin and the electron transport system of photosynthesis.

Subunit structure

Homodimer. Ref.3

Subcellular location

Plastidchloroplast.

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandNADP
   Molecular functionOxidoreductase
   PTMDisulfide bond
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmalate dehydrogenase (NADP+) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 6868Chloroplast
Chain69 – 453385Malate dehydrogenase [NADP], chloroplastic
PRO_0000018642

Regions

Nucleotide binding117 – 1237NADP
Nucleotide binding235 – 2373NADP

Sites

Active site2931Proton acceptor By similarity
Binding site1981Substrate By similarity
Binding site2041Substrate By similarity
Binding site2111NADP By similarity
Binding site2181NADP
Binding site2371Substrate By similarity
Binding site2681Substrate By similarity
Site881Activation of NADP-MDH By similarity
Site931Activation of NADP-MDH By similarity

Amino acid modifications

Disulfide bond88 ↔ 93In oxidized inactive NAD-MDH
Disulfide bond429 ↔ 441In oxidized inactive NAD-MDH

Secondary structure

............................................................. 453
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P46489 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 7CD3A0F9F2A7B539

FASTA45349,515
        10         20         30         40         50         60 
MAVVKLSPWA NYSSSKSEIK SSSSSSSSKS SLSAYVINVS SSPRLSFYNP YPRRLHHQRL 

        70         80         90        100        110        120 
SSPASIRCSV TSSDQIQAPL PAKQKPECFG VFCLTYDLKA EEETKSWKKI INVAVSGAAG 

       130        140        150        160        170        180 
MISNHLLFKL ASGEVFGPDQ PISLKLLGSE RSFAALEGVA MELEDSLYPL LRQVSIGIDP 

       190        200        210        220        230        240 
YEIFQDAEWA LLIGAKPRGP GMERADLLDI NGQIFAEQGK ALNAVASPNV KVMVVGNPCN 

       250        260        270        280        290        300 
TNALICLKNA PNIPPKNFHA LTRLDENRAK CQLALKAGVF YDKVSNVTIW GNHSTTQVPD 

       310        320        330        340        350        360 
FLNAKIHGIP VTEVIRDRKW LEDEFTNMVQ TRGGVLIKKW GRSSAASTAV SIVDAIRSLV 

       370        380        390        400        410        420 
TPTPEGDWFS TGVYTNGNPY GIAEDIVFSM PCRSKGDGDY EFVKDVIFDD YLSKKIKKSE 

       430        440        450 
DELLAEKKCV AHLTGEGIAV CDLPEDTMLP GEM 

« Hide

References

[1]Trevanion S.J., Ashton A.R.
Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Leaf.
[2]"Crystallization and preliminary crystallographic studies of chloroplast NADP-dependent malate dehydrogenase from Flaveria bidentis."
Macpherson K.H., Ashton A.R., Carr P.D., Trevanion S.J., Verger D., Ollis D.L.
Acta Crystallogr. D 54:654-656(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: CRYSTALLIZATION.
[3]"Chloroplast NADP-malate dehydrogenase: structural basis of light-dependent regulation of activity by thiol oxidation and reduction."
Carr P.D., Verger D., Ashton A.R., Ollis D.L.
Structure 7:461-475(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH NADP, SUBUNIT.
Tissue: Leaf.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L40958 mRNA. Translation: AAA63907.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1CIVX-ray2.80A69-453[»]
ProteinModelPortalP46489.
SMRP46489. Positions 80-453.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProIPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011273. Malate_DH_NADP-dep_pln.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR23382. PTHR23382. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
SUPFAMSSF56327. SSF56327. 1 hit.
TIGRFAMsTIGR01757. Malate-DH_plant. 1 hit.
TIGR01759. MalateDH-SF1. 1 hit.
PROSITEPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP46489.

Entry information

Entry nameMDHP_FLABI
AccessionPrimary (citable) accession number: P46489
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 19, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references