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P46489

- MDHP_FLABI

UniProt

P46489 - MDHP_FLABI

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Protein
Malate dehydrogenase [NADP], chloroplastic
Gene
N/A
Organism
Flaveria bidentis (Coastal plain yellowtops) (Ethulia bidentis)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

The chloroplastic, NADP-dependent form is essential for the photosynthesis C4 cycle, which allows plants to circumvent the problem of photorespiration. In C4 plants, NADP-MDH activity acts to convert oxaloacetate to malate in chloroplasts of mesophyll cells for transport to the bundle sheath cells.

Catalytic activityi

(S)-malate + NADP+ = oxaloacetate + NADPH.

Enzyme regulationi

Chloroplast NADP-MDH is activated upon illumination. In order to be enzymatically active, disulfide bridges on the protein must be reduced by thioredoxin which receives electrons from ferredoxin and the electron transport system of photosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei88 – 881Activation of NADP-MDH By similarity
Sitei93 – 931Activation of NADP-MDH By similarity
Binding sitei198 – 1981Substrate By similarity
Binding sitei204 – 2041Substrate By similarity
Binding sitei211 – 2111NADP By similarity
Binding sitei218 – 2181NADP
Binding sitei237 – 2371Substrate By similarity
Binding sitei268 – 2681Substrate By similarity
Active sitei293 – 2931Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi117 – 1237NADP
Nucleotide bindingi235 – 2373NADP

GO - Molecular functioni

  1. malate dehydrogenase (NADP+) activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase [NADP], chloroplastic (EC:1.1.1.82)
Alternative name(s):
NADP-MDH
OrganismiFlaveria bidentis (Coastal plain yellowtops) (Ethulia bidentis)
Taxonomic identifieri4224 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsAsteralesAsteraceaeAsteroideaeHeliantheae allianceTageteaeFlaveria

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6868Chloroplast
Add
BLAST
Chaini69 – 453385Malate dehydrogenase [NADP], chloroplastic
PRO_0000018642Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi88 ↔ 93In oxidized inactive NAD-MDH
Disulfide bondi429 ↔ 441In oxidized inactive NAD-MDH

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni90 – 934
Beta strandi101 – 1033
Beta strandi111 – 1166
Turni117 – 1193
Helixi121 – 13111
Turni132 – 1365
Beta strandi142 – 1476
Helixi150 – 1523
Helixi153 – 16412
Turni165 – 1673
Beta strandi171 – 1788
Helixi180 – 1834
Turni184 – 1863
Beta strandi188 – 1925
Helixi204 – 22522
Beta strandi231 – 2344
Beta strandi236 – 2383
Helixi239 – 24810
Helixi255 – 2573
Beta strandi258 – 2603
Helixi263 – 27715
Beta strandi283 – 2853
Beta strandi288 – 2914
Beta strandi298 – 3003
Helixi311 – 3133
Helixi318 – 3236
Helixi325 – 3295
Helixi332 – 3387
Helixi345 – 36016
Beta strandi369 – 3746
Beta strandi384 – 39310
Beta strandi395 – 3984
Helixi410 – 42920
Turni430 – 4356

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CIVX-ray2.80A69-453[»]
ProteinModelPortaliP46489.
SMRiP46489. Positions 80-453.

Miscellaneous databases

EvolutionaryTraceiP46489.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011273. Malate_DH_NADP-dep_pln.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01757. Malate-DH_plant. 1 hit.
TIGR01759. MalateDH-SF1. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46489-1 [UniParc]FASTAAdd to Basket

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MAVVKLSPWA NYSSSKSEIK SSSSSSSSKS SLSAYVINVS SSPRLSFYNP    50
YPRRLHHQRL SSPASIRCSV TSSDQIQAPL PAKQKPECFG VFCLTYDLKA 100
EEETKSWKKI INVAVSGAAG MISNHLLFKL ASGEVFGPDQ PISLKLLGSE 150
RSFAALEGVA MELEDSLYPL LRQVSIGIDP YEIFQDAEWA LLIGAKPRGP 200
GMERADLLDI NGQIFAEQGK ALNAVASPNV KVMVVGNPCN TNALICLKNA 250
PNIPPKNFHA LTRLDENRAK CQLALKAGVF YDKVSNVTIW GNHSTTQVPD 300
FLNAKIHGIP VTEVIRDRKW LEDEFTNMVQ TRGGVLIKKW GRSSAASTAV 350
SIVDAIRSLV TPTPEGDWFS TGVYTNGNPY GIAEDIVFSM PCRSKGDGDY 400
EFVKDVIFDD YLSKKIKKSE DELLAEKKCV AHLTGEGIAV CDLPEDTMLP 450
GEM 453
Length:453
Mass (Da):49,515
Last modified:November 1, 1995 - v1
Checksum:i7CD3A0F9F2A7B539
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L40958 mRNA. Translation: AAA63907.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L40958 mRNA. Translation: AAA63907.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1CIV X-ray 2.80 A 69-453 [» ]
ProteinModelPortali P46489.
SMRi P46489. Positions 80-453.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P46489.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProi IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011273. Malate_DH_NADP-dep_pln.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR23382. PTHR23382. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01757. Malate-DH_plant. 1 hit.
TIGR01759. MalateDH-SF1. 1 hit.
PROSITEi PS00068. MDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Trevanion S.J., Ashton A.R.
    Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Leaf.
  2. "Crystallization and preliminary crystallographic studies of chloroplast NADP-dependent malate dehydrogenase from Flaveria bidentis."
    Macpherson K.H., Ashton A.R., Carr P.D., Trevanion S.J., Verger D., Ollis D.L.
    Acta Crystallogr. D 54:654-656(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CRYSTALLIZATION.
  3. "Chloroplast NADP-malate dehydrogenase: structural basis of light-dependent regulation of activity by thiol oxidation and reduction."
    Carr P.D., Verger D., Ashton A.R., Ollis D.L.
    Structure 7:461-475(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH NADP, SUBUNIT.
    Tissue: Leaf.

Entry informationi

Entry nameiMDHP_FLABI
AccessioniPrimary (citable) accession number: P46489
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 19, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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