Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P46488 (MDHG_CUCSA)

Last modified October 13, 2009. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Malate dehydrogenase, glyoxysomal
    EC=1.1.1.37
Gene names
Name: MDHG
OrganismCucumis sativus (Cucumber)
Taxonomic identifier3659 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Cucurbitales › Cucurbitaceae › Cucumis

Hide | Top

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

Hide | Top

General annotation (Comments)

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH.

Subunit structure

Homodimer By similarity.

Subcellular location

Glyoxysome.

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 1 family.

Hide | Top

Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Cellular componentGlyoxysome
Peroxisome
   DomainTransit peptide
   LigandNAD
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological processglycolysis

Inferred from electronic annotation. Source: InterPro

glyoxylate cycle

Inferred from electronic annotation. Source: UniProtKB-KW

malate metabolic process

Inferred from electronic annotation. Source: InterPro

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentglyoxysome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: EC

binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3636Glyoxysome Potential
Chain37 – 356320Malate dehydrogenase, glyoxysomal
PRO_0000018639

Regions

Nucleotide binding51 – 577NAD By similarity
Nucleotide binding160 – 1623NAD By similarity

Sites

Active site2201Proton acceptor By similarity
Binding site771NAD By similarity
Binding site1241Substrate By similarity
Binding site1301Substrate By similarity
Binding site1371NAD By similarity
Binding site1621Substrate By similarity
Binding site1961Substrate By similarity
Binding site2711NAD By similarity

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
P46488-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 78695445C8D9E7B1

FASTA35637,739
        10         20         30         40         50         60 
MQPIPDVNQR IARISAHLHP PKYQMEESSV LRRANCRAKG GAPGFKVAIL GAAGGIGQPL 

        70         80         90        100        110        120 
AMLMKMNPLV SVLHLYDVVN APGVTADISH MDTGAVVRGF LGQQQLERAL TGMDLVVIPA 

       130        140        150        160        170        180 
GVPRKPGMTR DDLFKINAGI VKTLCEGIAK CCPTAIVNLI SNPVNSTVPI AAEVFKKAGT 

       190        200        210        220        230        240 
YDPKRLLGVT MLDVVRANTF VAEVLGLDPR DVNVPVVGGH AGVTILPLLS QVKPPSSFTQ 

       250        260        270        280        290        300 
EEINYLTDRI QNGGTEVVEA KAGAGSATLS MAYAAVKFAD ACLRGLRGDA GVVECAFVSS 

       310        320        330        340        350 
QVTELPFFAT KVRLGRNGID EVYSLGPLNE YERIGLEKAK KELAGSIEKG VSFIRG

« Hide

Hide | Top

References

[1]"Expression of a single gene encoding microbody NAD-malate dehydrogenase during glyoxysome and peroxisome development in cucumber."
Kim D.J., Smith S.M.
Plant Mol. Biol. 26:1833-1841(1994) [PubMed: 7858221] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Masterpiece.
Tissue: Cotyledon.

Hide | Top

Cross-references

Sequence databases

L31900 mRNA. Translation: AAC41647.1.
PIRS52039.

3D structure databases

HSSPHSSP built from PDB template 1MLD based on UniProtKB P00346.
SMRP46488. Positions 44-355.
ModBaseSearch...

Enzyme and pathway databases

BRENDA1.1.1.37. 1241.

Family and domain databases

InterProIPR001557. L-lactate/malate_DH.
IPR001236. Lactate/malate_DH.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_NAD-dep_euk/g-bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.90.110.10. lact_mal_DH. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHERPTHR11540:SF1. MDH_euk_g_bac. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
TIGRFAMsTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Hide | Top

Entry information

Entry nameMDHG_CUCSA
AccessionPrimary (citable) accession number: P46488
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 13, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Hide | Top

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents