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Protein

Malate dehydrogenase, glyoxysomal

Gene

MDHG

Organism
Cucumis sativus (Cucumber)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei77 – 771NADBy similarity
Binding sitei124 – 1241SubstratePROSITE-ProRule annotation
Binding sitei130 – 1301SubstratePROSITE-ProRule annotation
Binding sitei137 – 1371NADBy similarity
Binding sitei162 – 1621SubstratePROSITE-ProRule annotation
Binding sitei196 – 1961SubstratePROSITE-ProRule annotation
Active sitei220 – 2201Proton acceptorBy similarity
Binding sitei271 – 2711NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi51 – 577NADBy similarity
Nucleotide bindingi160 – 1623NADBy similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-KW
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, glyoxysomal (EC:1.1.1.37)
Gene namesi
Name:MDHG
OrganismiCucumis sativus (Cucumber)
Taxonomic identifieri3659 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeBenincaseaeCucumis

Subcellular locationi

GO - Cellular componenti

  1. glyoxysome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3636GlyoxysomeSequence AnalysisAdd
BLAST
Chaini37 – 356320Malate dehydrogenase, glyoxysomalPRO_0000018639Add
BLAST

Proteomic databases

PRIDEiP46488.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP46488.
SMRiP46488. Positions 44-355.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46488-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQPIPDVNQR IARISAHLHP PKYQMEESSV LRRANCRAKG GAPGFKVAIL
60 70 80 90 100
GAAGGIGQPL AMLMKMNPLV SVLHLYDVVN APGVTADISH MDTGAVVRGF
110 120 130 140 150
LGQQQLERAL TGMDLVVIPA GVPRKPGMTR DDLFKINAGI VKTLCEGIAK
160 170 180 190 200
CCPTAIVNLI SNPVNSTVPI AAEVFKKAGT YDPKRLLGVT MLDVVRANTF
210 220 230 240 250
VAEVLGLDPR DVNVPVVGGH AGVTILPLLS QVKPPSSFTQ EEINYLTDRI
260 270 280 290 300
QNGGTEVVEA KAGAGSATLS MAYAAVKFAD ACLRGLRGDA GVVECAFVSS
310 320 330 340 350
QVTELPFFAT KVRLGRNGID EVYSLGPLNE YERIGLEKAK KELAGSIEKG

VSFIRG
Length:356
Mass (Da):37,739
Last modified:November 1, 1995 - v1
Checksum:i78695445C8D9E7B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31900 mRNA. Translation: AAC41647.1.
PIRiS52039.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31900 mRNA. Translation: AAC41647.1.
PIRiS52039.

3D structure databases

ProteinModelPortaliP46488.
SMRiP46488. Positions 44-355.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP46488.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Expression of a single gene encoding microbody NAD-malate dehydrogenase during glyoxysome and peroxisome development in cucumber."
    Kim D.J., Smith S.M.
    Plant Mol. Biol. 26:1833-1841(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Masterpiece.
    Tissue: Cotyledon.

Entry informationi

Entry nameiMDHG_CUCSA
AccessioniPrimary (citable) accession number: P46488
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 7, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.