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P46488

- MDHG_CUCSA

UniProt

P46488 - MDHG_CUCSA

Protein

Malate dehydrogenase, glyoxysomal

Gene

MDHG

Organism
Cucumis sativus (Cucumber)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei77 – 771NADBy similarity
    Binding sitei124 – 1241SubstratePROSITE-ProRule annotation
    Binding sitei130 – 1301SubstratePROSITE-ProRule annotation
    Binding sitei137 – 1371NADBy similarity
    Binding sitei162 – 1621SubstratePROSITE-ProRule annotation
    Binding sitei196 – 1961SubstratePROSITE-ProRule annotation
    Active sitei220 – 2201Proton acceptorBy similarity
    Binding sitei271 – 2711NADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi51 – 577NADBy similarity
    Nucleotide bindingi160 – 1623NADBy similarity

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-KW
    2. malate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenase, glyoxysomal (EC:1.1.1.37)
    Gene namesi
    Name:MDHG
    OrganismiCucumis sativus (Cucumber)
    Taxonomic identifieri3659 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeBenincaseaeCucumis

    Subcellular locationi

    GO - Cellular componenti

    1. glyoxysome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Glyoxysome, Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3636GlyoxysomeSequence AnalysisAdd
    BLAST
    Chaini37 – 356320Malate dehydrogenase, glyoxysomalPRO_0000018639Add
    BLAST

    Proteomic databases

    PRIDEiP46488.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP46488.
    SMRiP46488. Positions 44-355.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010097. Malate_DH_type1.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11540. PTHR11540. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
    PROSITEiPS00068. MDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P46488-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQPIPDVNQR IARISAHLHP PKYQMEESSV LRRANCRAKG GAPGFKVAIL    50
    GAAGGIGQPL AMLMKMNPLV SVLHLYDVVN APGVTADISH MDTGAVVRGF 100
    LGQQQLERAL TGMDLVVIPA GVPRKPGMTR DDLFKINAGI VKTLCEGIAK 150
    CCPTAIVNLI SNPVNSTVPI AAEVFKKAGT YDPKRLLGVT MLDVVRANTF 200
    VAEVLGLDPR DVNVPVVGGH AGVTILPLLS QVKPPSSFTQ EEINYLTDRI 250
    QNGGTEVVEA KAGAGSATLS MAYAAVKFAD ACLRGLRGDA GVVECAFVSS 300
    QVTELPFFAT KVRLGRNGID EVYSLGPLNE YERIGLEKAK KELAGSIEKG 350
    VSFIRG 356
    Length:356
    Mass (Da):37,739
    Last modified:November 1, 1995 - v1
    Checksum:i78695445C8D9E7B1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L31900 mRNA. Translation: AAC41647.1.
    PIRiS52039.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L31900 mRNA. Translation: AAC41647.1 .
    PIRi S52039.

    3D structure databases

    ProteinModelPortali P46488.
    SMRi P46488. Positions 44-355.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P46488.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010097. Malate_DH_type1.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11540. PTHR11540. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01772. MDH_euk_gproteo. 1 hit.
    PROSITEi PS00068. MDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression of a single gene encoding microbody NAD-malate dehydrogenase during glyoxysome and peroxisome development in cucumber."
      Kim D.J., Smith S.M.
      Plant Mol. Biol. 26:1833-1841(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Masterpiece.
      Tissue: Cotyledon.

    Entry informationi

    Entry nameiMDHG_CUCSA
    AccessioniPrimary (citable) accession number: P46488
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3