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P46487 (MDHM_EUCGU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase, mitochondrial

EC=1.1.1.37
Gene names
Name:MDH
OrganismEucalyptus gunnii (Cider gum)
Taxonomic identifier3933 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsMyrtalesMyrtaceaeEucalyptus

Protein attributes

Sequence length347 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH.

Subunit structure

Homodimer By similarity.

Subcellular location

Mitochondrion matrix.

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 1 family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   Cellular componentMitochondrion
   DomainTransit peptide
   LigandNAD
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentmitochondrial matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2727Mitochondrion Potential
Chain28 – 347320Malate dehydrogenase, mitochondrial
PRO_0000018626

Regions

Nucleotide binding41 – 477NAD By similarity
Nucleotide binding150 – 1523NAD By similarity

Sites

Active site2101Proton acceptor By similarity
Binding site671NAD By similarity
Binding site1141Substrate By similarity
Binding site1201Substrate By similarity
Binding site1271NAD By similarity
Binding site1521Substrate By similarity
Binding site1861Substrate By similarity
Binding site2611NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
P46487 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 450F8254CCC84789

FASTA34736,462
        10         20         30         40         50         60 
MRASMLRLIR SRSSSAAPRP HLLRRAYGSE SVPERKVAVL GAAGGIGQPL ALLMKLNPLV 

        70         80         90        100        110        120 
SQLALYDIAG TPGVAADVGH INTRSEVAGY VGEEQLGQAL EGSDVVIIPA GVPRKPGMTR 

       130        140        150        160        170        180 
DDLFNINAGI VKSLCTAIAK YCPNAVVNMI SNPVNSTVPI AAEIFKKAGT YNEKKLLGVT 

       190        200        210        220        230        240 
TLDVVRAKTF YAGKAKVPVE EVNVPVVGGH AGITILPLFS QAVPKANLAD EDIKALTKRT 

       250        260        270        280        290        300 
QDGGTEVVEA KAGKGSATLS MAYAGALFAD ACLKGLNGVP DVVECSFVQS SIITELPFFA 

       310        320        330        340 
SKVKLGKNGV EEVLELGPMS DYEKQGLEIL IPELKASIEK GIKFANQ 

« Hide

References

[1]"Nucleotide sequence of a cDNA encoding mitochondrial malate dehydrogenase from Eucalyptus."
Poeydomenge O., Boudet A.M., Grima-Pettenati J., Marolda M.
Plant Physiol. 107:1455-1456(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X78800 mRNA. Translation: CAA55383.1.
PIRS44167.

3D structure databases

ProteinModelPortalP46487.
SMRP46487. Positions 36-344.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP46487.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11540. PTHR11540. 1 hit.
PTHR11540:SF1. PTHR11540:SF1. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMSSF56327. SSF56327. 1 hit.
TIGRFAMsTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMDHM_EUCGU
AccessionPrimary (citable) accession number: P46487
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 19, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families