Skip Header

 
Contribute Send feedback

Reviewed, UniProtKB/Swiss-Prot P46487 (MDHM_EUCGU)

Last modified November 25, 2008. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Malate dehydrogenase, mitochondrial
    EC=1.1.1.37
Gene names
Name: MDH
OrganismEucalyptus gunnii (Cider tree)
Taxonomic identifier3933 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › Myrtales › Myrtaceae › Eucalyptus

Hide | Top

Protein attributes

Sequence length347 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

Hide | Top

General annotation (Comments)

Catalytic activity

(S)-malate + NAD(+) = oxaloacetate + NADH.

Subunit structure

Homodimer By similarity.

Subcellular location

Mitochondrion matrix.

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 1 family.

Hide | Top

Ontologies

Keywords

   Biological processTricarboxylic acid cycle
   Cellular componentMitochondrion
   DomainTransit peptide
   LigandNAD
   Molecular functionOxidoreductase

Gene Ontology (GO)

   Biological processglycolysis

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentmitochondrial matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: InterPro

binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2727Mitochondrion Potential
Chain28 – 347320Malate dehydrogenase, mitochondrial
PRO_0000018626

Regions

Nucleotide binding41 – 477NAD By similarity
Nucleotide binding150 – 1523NAD By similarity

Sites

Active site2101Proton acceptor By similarity
Binding site671NAD By similarity
Binding site1141Substrate By similarity
Binding site1201Substrate By similarity
Binding site1271NAD By similarity
Binding site1521Substrate By similarity
Binding site1861Substrate By similarity
Binding site2611NAD By similarity

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
P46487-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 450F8254CCC84789

FASTA34736,462
        10         20         30         40         50         60 
MRASMLRLIR SRSSSAAPRP HLLRRAYGSE SVPERKVAVL GAAGGIGQPL ALLMKLNPLV 

        70         80         90        100        110        120 
SQLALYDIAG TPGVAADVGH INTRSEVAGY VGEEQLGQAL EGSDVVIIPA GVPRKPGMTR 

       130        140        150        160        170        180 
DDLFNINAGI VKSLCTAIAK YCPNAVVNMI SNPVNSTVPI AAEIFKKAGT YNEKKLLGVT 

       190        200        210        220        230        240 
TLDVVRAKTF YAGKAKVPVE EVNVPVVGGH AGITILPLFS QAVPKANLAD EDIKALTKRT 

       250        260        270        280        290        300 
QDGGTEVVEA KAGKGSATLS MAYAGALFAD ACLKGLNGVP DVVECSFVQS SIITELPFFA 

       310        320        330        340 
SKVKLGKNGV EEVLELGPMS DYEKQGLEIL IPELKASIEK GIKFANQ

« Hide

Hide | Top

References

[1]"Nucleotide sequence of a cDNA encoding mitochondrial malate dehydrogenase from Eucalyptus."
Poeydomenge O., Boudet A.M., Grima-Pettenati J., Marolda M.
Plant Physiol. 107:1455-1456(1995) [PubMed: 7770532] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Hide | Top

Cross-references

Sequence databases

X78800 mRNA. Translation: CAA55383.1.
PIRS44167.

3D structure databases

HSSPHSSP built from PDB template 1MLD based on UniProtKB P00346.
ModBaseSearch...

Family and domain databases

InterProIPR001557. L-lactate/malate_DHase.
IPR001236. Lactate/malate_DHase.
IPR015955. Lactate_DHase/Glyco_Ohase_4_C.
IPR001252. Malate_DHase_AS.
IPR010097. Malate_DHase_NAD-dep_euk_g_bac.
IPR016040. NAD(P)-bd.
[Graphical view]
Gene3DG3DSA:3.90.110.10. lact_mal_DH. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHERPTHR11540:SF1. MDH_euk_g_bac. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
TIGRFAMsTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Hide | Top

Entry information

Entry nameMDHM_EUCGU
AccessionPrimary (citable) accession number: P46487
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 25, 2008
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Hide | Top

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents