Reviewed, UniProtKB/Swiss-Prot P46482 (AAEA_ECOLI)

Names and origin

Protein names

Recommended name:
    p-hydroxybenzoic acid efflux pump subunit aaeA
      Short name=pHBA efflux pump protein A

Gene names

Name:	aaeA
Synonyms:	yhcQ
Ordered Locus Names:	b3241, JW3210

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Subcellular location	Cell inner membrane; Single-pass membrane protein Potential.
Induction	Positively coregulated with aaeB and aaeX by aaeR. HAMAP MF_01544
Miscellaneous	Both aaeA and aaeB are required for the function of the efflux system. HAMAP MF_01544
Sequence similarities	Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein secretion Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	p-hydroxybenzoic acid efflux pump subunit aaeA HAMAP MF_01544		PRO_0000201850
Regions
	Transmembrane	12 – 32	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	P46482-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 6E0232D459DC2757	FASTA	310	34,775
	10 20 30 40 50 60 MKTLIRKFSR TAITVVLVIL AFIAIFNAWV YYTESPWTRD ARFSADVVAI APDVSGLITQ 70 80 90 100 110 120 VNVHDNQLVK KGQILFTIDQ PRYQKALEEA QADVAYYQVL AQEKRQEAGR RNRLGVQAMS 130 140 150 160 170 180 REEIDQANNV LQTVLHQLAK AQATRDLAKL DLERTVIRAP ADGWVTNLNV YTGEFITRGS 190 200 210 220 230 240 TAVALVKQNS FYVLAYMEET KLEGVRPGYR AEITPLGSNK VLKGTVDSVA AGVTNASSTR 250 260 270 280 290 300 DDKGMATIDS NLEWVRLAQR VPVRIRLDNQ QENIWPAGTT ATVVVTGKQD RDESQDSFFR 310 KMAHRLREFG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Characterization of the Escherichia coli AaeAB efflux pump: a metabolic relief valve?" Van Dyk T.K., Templeton L.J., Cantera K.A., Sharpe P.L., Sariaslani F.S. J. Bacteriol. 186:7196-7204(2004) [PubMed: 15489430] [Abstract] Cited for: INVOLVEMENT IN THE AAEAB EFFLUX SYSTEM. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U18997 Genomic DNA. Translation: AAA58043.1. U00096 Genomic DNA. Translation: AAC76273.1. AP009048 Genomic DNA. Translation: BAE77284.1.
PIR	C65116.
RefSeq	AP_003783.1. NP_417708.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:12289N.
Protein family/group databases
TCDB	8.A.1.7.1. membrane fusion protein (MFP) family.
Genome annotation databases
GeneID	947748.
GenomeReviews	Gene locus JW3210 in contig AP009048_GR. Gene locus b3241 in contig U00096_GR.
KEGG	ecj:JW3210. eco:b3241.
Organism-specific databases
EchoBASE	EB2674.
EcoGene	EG12823. aaeA.
CMR	Search...
Phylogenomic databases
HOGENOM	P46482.
OMA	P46482. FRIDRER.
Enzyme and pathway databases
BioCyc	EcoCyc:G7686-MON.
Family and domain databases
HAMAP	MF_01544. [Tree]
InterPro	IPR006143. Secretion_HlyD. [Graphical view]
Pfam	PF00529. HlyD. 1 hit. [Graphical view]
TIGRFAMs	TIGR01730. RND_mfp. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AAEA_ECOLI

Accession

Primary (citable) accession number: P46482
Secondary accession number(s): Q2M8X2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)