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P46481 (AAEB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
p-hydroxybenzoic acid efflux pump subunit AaeB
Short name=pHBA efflux pump protein B
Gene names
Name:aaeB
Synonyms:yhcP
Ordered Locus Names:b3240, JW3209
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length655 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Efflux pump whose substrates are p-hydroxybenzoic acid (pHBA), 6-hydroxy-2-naphthoic and 2-hydroxycinnamate. Could function as a metabolic relief valve, allowing to eliminate certain compounds when they accumulate to high levels in the cell. Ref.3

Subcellular location

Cell inner membrane; Multi-pass membrane protein HAMAP MF_01545.

Induction

Positively coregulated with aaeA and aaeX by AaeR. HAMAP MF_01545

Miscellaneous

Both AaeB and AaeA are required for the function of the efflux system. HAMAP MF_01545

Sequence similarities

Belongs to the aromatic acid exporter ArAE (TC 2.A.85) family. [View classification]

Ontologies

Keywords
   Biological processTransport
   Cellular componentCell inner membrane
Cell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processcarboxylic acid transport

Inferred from mutant phenotype Ref.3. Source: EcoliWiki

response to chemical stimulus

Inferred from genetic interaction Ref.3. Source: EcoliWiki

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 655655p-hydroxybenzoic acid efflux pump subunit AaeB HAMAP MF_01545
PRO_0000210077

Regions

Topological domain1 – 1212Periplasmic Potential
Transmembrane13 – 3321Helical; Potential
Topological domain34 – 374Cytoplasmic Potential
Transmembrane38 – 5821Helical; Potential
Topological domain59 – 6810Periplasmic Potential
Transmembrane69 – 8921Helical; Potential
Topological domain90 – 923Cytoplasmic Potential
Transmembrane93 – 11321Helical; Potential
Topological domain114 – 1207Periplasmic Potential
Transmembrane121 – 14121Helical; Potential
Topological domain142 – 15110Cytoplasmic Potential
Transmembrane152 – 17221Helical; Potential
Topological domain173 – 369197Periplasmic Potential
Transmembrane370 – 39021Helical; Potential
Topological domain391 – 40616Cytoplasmic Potential
Transmembrane407 – 42721Helical; Potential
Topological domain428 – 4303Periplasmic Potential
Transmembrane431 – 45121Helical; Potential
Topological domain452 – 4587Cytoplasmic Potential
Transmembrane459 – 47921Helical; Potential
Topological domain480 – 4812Periplasmic Potential
Transmembrane482 – 50221Helical; Potential
Topological domain503 – 655153Cytoplasmic Potential

Sequences

Sequence LengthMass (Da)Tools
P46481 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 6C559FC19C276656

FASTA65573,591
        10         20         30         40         50         60 
MGIFSIANQH IRFAVKLATA IVLALFVGFH FQLETPRWAV LTAAIVAAGT AFAAGGEPYS 

        70         80         90        100        110        120 
GAIRYRGFLR IIGTFIGCIA GLVIIIAMIR APLLMILVCC IWAGFCTWIS SLVRIENSYA 

       130        140        150        160        170        180 
WGLAGYTALI IVITIQPEPL LTPQFAVERC SEIVIGIVCA IMADLLFSPR SIKQEVDREL 

       190        200        210        220        230        240 
ESLLVAQYQL MQLCIKHGDG EVVDKAWGDL VRRTTALQGM RSNLNMESSR WARANRRLKA 

       250        260        270        280        290        300 
INTLSLTLIT QSCETYLIQN TRPELITDTF REFFDTPVET AQDVHKQLKR LRRVIAWTGE 

       310        320        330        340        350        360 
RETPVTIYSW VAAATRYQLL KRGVISNTKI NATEEEILQG EPEVKVESAE RHHAMVNFWR 

       370        380        390        400        410        420 
TTLSCILGTL FWLWTGWTSG SGAMVMIAVV TSLAMRLPNP RMVAIDFIYG TLAALPLGLL 

       430        440        450        460        470        480 
YFLVIIPNTQ QSMLLLCISL AVLGFFLGIE VQKRRLGSMG ALASTINIIV LDNPMTFHFS 

       490        500        510        520        530        540 
QFLDSALGQI VGCVLAFTVI LLVRDKSRDR TGRVLLNQFV SAAVSAMTTN VARRKENHLP 

       550        560        570        580        590        600 
ALYQQLFLLM NKFPGDLPKF RLALTMIIAH QRLRDAPIPV NEDLSAFHRQ MRRTADHVIS 

       610        620        630        640        650 
ARSDDKRRRY FGQLLEELEI YQEKLRIWQA PPQVTEPVNR LAGMLHKYQH ALTDS

« Hide

References

« Hide 'large scale' references
[1]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"Characterization of the Escherichia coli AaeAB efflux pump: a metabolic relief valve?"
Van Dyk T.K., Templeton L.J., Cantera K.A., Sharpe P.L., Sariaslani F.S.
J. Bacteriol. 186:7196-7204(2004) [PubMed: 15489430] [Abstract]
Cited for: FUNCTION.
Strain: K12 / MG1655 / ATCC 47076.
[4]"Global topology analysis of the Escherichia coli inner membrane proteome."
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U18997 Genomic DNA. Translation: AAA58042.1.
U00096 Genomic DNA. Translation: AAC76272.1.
AP009048 Genomic DNA. Translation: BAE77283.1.
PIRB65116.
RefSeqNP_417707.1. NC_000913.2.

3D structure databases

ProteinModelPortalP46481.
ModBaseSearch...

Protein-protein interaction databases

IntActP46481. 2 interactions.

Protein family/group databases

TCDB2.A.85.1.2. aromatic acid exporter (ArAE) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000002073; EBESCP00000002073; EBESCG00000001693.
EBESCT00000017564; EBESCP00000016855; EBESCG00000016620.
GeneID947747.
GenomeReviewsGene locus JW3209 in contig AP009048_GR.
Gene locus b3240 in contig U00096_GR.
KEGGecj:JW3209.
eco:b3240.
PATRIC32121906. VBIEscCol129921_3337.

Organism-specific databases

EchoBASEEB2673.
EcoGeneEG12822. aaeB.

Phylogenomic databases

eggNOGCOG1289.
GeneTreeEBGT00050000010059.
HOGENOMHBG417090.
OMALCCIWAG.
PhylomeDBP46481.
ProtClustDBPRK10631.

Enzyme and pathway databases

BioCycEcoCyc:G7685-MONOMER.

Gene expression databases

GenevestigatorP46481.

Family and domain databases

HAMAPMF_01545. AaeB.
[Tree]
InterProIPR006726. PHBA_efflux_AaeB/fusaric-R.
IPR023706. PHBA_efflux_pump_AaeB.
[Graphical view]
KOK03468.
PfamPF04632. FUSC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAAEB_ECOLI
AccessionPrimary (citable) accession number: P46481
Secondary accession number(s): Q2M8X3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 25, 2012
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents