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Protein

26S protease regulatory subunit 7

Gene

Psmc2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC2 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi216 – 223ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
26S protease regulatory subunit 7
Alternative name(s):
26S proteasome AAA-ATPase subunit RPT1
Proteasome 26S subunit ATPase 2
Protein MSS1
Gene namesi
Name:Psmc2
Synonyms:Mss1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:109555. Psmc2.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000847102 – 43326S protease regulatory subunit 7Add BLAST432

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei116N6-acetyllysineBy similarity1
Modified residuei422N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP46471.
MaxQBiP46471.
PaxDbiP46471.
PeptideAtlasiP46471.
PRIDEiP46471.

2D gel databases

REPRODUCTION-2DPAGEP46471.
SWISS-2DPAGEP46471.

PTM databases

iPTMnetiP46471.
PhosphoSitePlusiP46471.
SwissPalmiP46471.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028932.

Interactioni

Subunit structurei

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases including PSMC2 and few additional components. Interacts with NDC80 and SQSTM1. Interacts with PAAF1. Interacts with HIV-1 Tat. Interacts with TRIM5.By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

IntActiP46471. 5 interactors.
MINTiMINT-8223516.
STRINGi10090.ENSMUSP00000030769.

Structurei

3D structure databases

ProteinModelPortaliP46471.
SMRiP46471.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Phylogenomic databases

eggNOGiKOG0729. Eukaryota.
COG1222. LUCA.
HOVERGENiHBG000109.
InParanoidiP46471.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiView protein in InterPro
IPR005937. 26S_Psome_P45.
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00004. AAA. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
PROSITEiView protein in PROSITE
PS00674. AAA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46471-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPDYLGADQR KTKEEEKDDK PIRALDEGDI ALLKTYGQST YSRQIKQVED
60 70 80 90 100
DIQQLLKKIN ELTGIKESDT GLAPPALWDL AADKQTLQSE QPLQVARCTK
110 120 130 140 150
IINADSEDPK YIINVKQFAK FVVDLSDQVA PTDIEEGMRV GVDRNKYQIH
160 170 180 190 200
IPLPPKIDPT VTMMQVEEKP DVTYSDVGGC KEQIEKLREV VETPLLHPER
210 220 230 240 250
FVNLGIEPPK GVLLFGPPGT GKTLCARAVA NRTDACFIRV IGSELVQKYV
260 270 280 290 300
GEGARMVREL FEMARTKKAC LIFFDEIDAI GGARFDDGAG GDNEVQRTML
310 320 330 340 350
ELINQLDGFD PRGNIKVLMA TNRPDTLDPA LMRPGRLDRK IEFSLPDLEG
360 370 380 390 400
RTHIFKIHAR SMSVERDIRF ELLARLCPNS TGAEIRSVCT EAGMFAIRAR
410 420 430
RKIATEKDFL EAVNKVIKSY AKFSATPRYM TYN
Length:433
Mass (Da):48,648
Last modified:January 23, 2007 - v5
Checksum:i302E4FA62A48104C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8D → E in AAB51069 (PubMed:9353061).Curated1
Sequence conflicti37 – 40GQST → VQRC in AAB51069 (PubMed:9353061).Curated4
Sequence conflicti37G → A no nucleotide entry (PubMed:8247131).Curated1
Sequence conflicti114N → D in AAB51069 (PubMed:9353061).Curated1
Sequence conflicti122 – 123VV → EE no nucleotide entry (PubMed:8247131).Curated2
Sequence conflicti138M → R in AAB51069 (PubMed:9353061).Curated1
Sequence conflicti148Q → P in AAB51069 (PubMed:9353061).Curated1
Sequence conflicti170P → L no nucleotide entry (PubMed:8247131).Curated1
Sequence conflicti172V → F no nucleotide entry (PubMed:8247131).Curated1
Sequence conflicti188R → L no nucleotide entry (PubMed:8247131).Curated1
Sequence conflicti201F → L no nucleotide entry (PubMed:8247131).Curated1
Sequence conflicti277I → M no nucleotide entry (PubMed:8247131).Curated1
Sequence conflicti369R → G no nucleotide entry (PubMed:8247131).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005083 mRNA. Translation: BAB23807.1.
AK168548 mRNA. Translation: BAE40423.1.
BC005462 mRNA. Translation: AAH05462.1.
U61283 mRNA. Translation: AAB51069.1.
PIRiS39349.
UniGeneiMm.2462.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005083 mRNA. Translation: BAB23807.1.
AK168548 mRNA. Translation: BAE40423.1.
BC005462 mRNA. Translation: AAH05462.1.
U61283 mRNA. Translation: AAB51069.1.
PIRiS39349.
UniGeneiMm.2462.

3D structure databases

ProteinModelPortaliP46471.
SMRiP46471.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP46471. 5 interactors.
MINTiMINT-8223516.
STRINGi10090.ENSMUSP00000030769.

PTM databases

iPTMnetiP46471.
PhosphoSitePlusiP46471.
SwissPalmiP46471.

2D gel databases

REPRODUCTION-2DPAGEP46471.
SWISS-2DPAGEP46471.

Proteomic databases

EPDiP46471.
MaxQBiP46471.
PaxDbiP46471.
PeptideAtlasiP46471.
PRIDEiP46471.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:109555. Psmc2.

Phylogenomic databases

eggNOGiKOG0729. Eukaryota.
COG1222. LUCA.
HOVERGENiHBG000109.
InParanoidiP46471.

Miscellaneous databases

PROiP46471.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028932.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiView protein in InterPro
IPR005937. 26S_Psome_P45.
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00004. AAA. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
PROSITEiView protein in PROSITE
PS00674. AAA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPRS7_MOUSE
AccessioniPrimary (citable) accession number: P46471
Secondary accession number(s): O08531, Q3TGX5, Q9DBA1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: February 15, 2017
This is version 142 of the entry and version 5 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.