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P46461

- NSF1_DROME

UniProt

P46461 - NSF1_DROME

Protein

Vesicle-fusing ATPase 1

Gene

comt

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi550 – 5501Magnesium

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi505 – 5106ATPBy similarity
    Nucleotide bindingi545 – 5528ATPBy similarity

    GO - Molecular functioni

    1. ATPase activity Source: FlyBase
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW
    4. protein complex binding Source: FlyBase

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. Golgi organization Source: FlyBase
    3. neuromuscular synaptic transmission Source: FlyBase
    4. neurotransmitter secretion Source: FlyBase
    5. phagocytosis Source: FlyBase
    6. positive regulation of SNARE complex disassembly Source: FlyBase
    7. protein hexamerization Source: FlyBase
    8. protein homooligomerization Source: FlyBase
    9. protein transport Source: UniProtKB-KW
    10. regulation of short-term neuronal synaptic plasticity Source: FlyBase
    11. synaptic vesicle fusion to presynaptic membrane Source: FlyBase
    12. synaptic vesicle priming Source: FlyBase

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    ER-Golgi transport, Protein transport, Transport

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vesicle-fusing ATPase 1 (EC:3.6.4.6)
    Alternative name(s):
    N-ethylmaleimide-sensitive fusion protein 1
    Short name:
    NEM-sensitive fusion protein 1
    Protein comatose
    Vesicular-fusion protein NSF1
    dNsf-1
    Short name:
    NSF-1
    Gene namesi
    Name:comt
    Synonyms:Nsf, Nsf1
    ORF Names:CG1618
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome X

    Organism-specific databases

    FlyBaseiFBgn0000346. comt.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: FlyBase
    2. terminal bouton Source: FlyBase

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 745745Vesicle-fusing ATPase 1PRO_0000084566Add
    BLAST

    Proteomic databases

    PaxDbiP46461.
    PRIDEiP46461.

    Expressioni

    Tissue specificityi

    Nervous system.

    Gene expression databases

    BgeeiP46461.

    Interactioni

    Subunit structurei

    Homohexamer.By similarity

    Protein-protein interaction databases

    BioGridi70577. 11 interactions.
    DIPiDIP-20666N.
    IntActiP46461. 1 interaction.
    MINTiMINT-288692.
    STRINGi7227.FBpp0073585.

    Structurei

    3D structure databases

    ProteinModelPortaliP46461.
    SMRiP46461. Positions 5-200, 214-738.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AAA ATPase family.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0464.
    GeneTreeiENSGT00530000064085.
    InParanoidiP46461.
    KOiK06027.
    OMAiESARQCE.
    OrthoDBiEOG7P8P7F.
    PhylomeDBiP46461.

    Family and domain databases

    Gene3Di3.10.330.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR009010. Asp_de-COase-like_dom.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR004201. Cdc48_dom2.
    IPR029067. CDC48_domain_2-like.
    IPR003338. CDC4_N-term_subdom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00004. AAA. 2 hits.
    PF02933. CDC48_2. 1 hit.
    PF02359. CDC48_N. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 2 hits.
    SM01072. CDC48_2. 1 hit.
    SM01073. CDC48_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF54585. SSF54585. 1 hit.
    PROSITEiPS00674. AAA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P46461-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAYILKATKC PTDELSLTNR AIVNVGDFPE EIKYADISPA PGQHFIFALE    50
    KTVEVPSGYV GFSLVQRKWA MVSINQELEV RPYRFDASSD VITCVSFETD 100
    FLQKKTVSQE PYDSDQMAKE FIMQFAGMAL TVGQSLVFNF KDKKLLGLAV 150
    KSLEAIDPKS LGEGKDTAMR NVRFGRILGN TVVQFEKAEN SSLNLQGKSK 200
    GKVVRQSIIN PDWDFGKMGI GGLDKEFNSI FRRAFASRVF PPELVEQLGC 250
    KHVKGILLYG PPGTGKTLMA RQIGTMLNAR EPKIVNGPQI LDKYVGESEA 300
    NVRRLFAEAE EEEKRLGPNS GLHIIIFDEI DAICKQRGSV AGNSGVHDTV 350
    VNQLLTKIDG VDQLNNILVI GMTNRRDMID EALLRPGRLE VQMEISLPNE 400
    QGRVQILNIH TKRMREFNKI NDDVDNKEIA ALTKNFSGAE LEGLVRAAQS 450
    SAMNRLIKAD AKVTVDPEAM EKLKVNRDDF LHSLEHDIKP AFGTAQEILD 500
    NMLARGVINW GAPVSNLLED GMLYVQQAKA PESSGLVSVL VAGAPNSGKT 550
    ALAAQLAKMS DFPFVKVCSP EDMVGYTESA KCLHIRKIFD DAYRSMLSCI 600
    VVDNVERLLD YGSIGPRYSN MTLQALLVLL KKQPPKGRKL LILCTSSRRE 650
    VLEEMEMLTA FTSVLHVPNL SKPDHVLAVL ENTDIFSKGE IQAIGKKMAG 700
    KRVFIGIKKL LGLIDMARQT EQSQRAIKFL SKMEEEGGLD MVARQ 745
    Length:745
    Mass (Da):82,555
    Last modified:November 1, 1995 - v1
    Checksum:iA34E7B037E91769F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09373 mRNA. Translation: AAA83413.1.
    AE014298 Genomic DNA. Translation: AAF48244.2.
    BT010259 mRNA. Translation: AAQ23577.1.
    RefSeqiNP_001259506.1. NM_001272577.1.
    NP_524877.1. NM_080138.3.
    UniGeneiDm.1772.

    Genome annotation databases

    EnsemblMetazoaiFBtr0073754; FBpp0073585; FBgn0000346.
    FBtr0333329; FBpp0305521; FBgn0000346.
    GeneIDi47091.
    KEGGidme:Dmel_CG1618.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09373 mRNA. Translation: AAA83413.1 .
    AE014298 Genomic DNA. Translation: AAF48244.2 .
    BT010259 mRNA. Translation: AAQ23577.1 .
    RefSeqi NP_001259506.1. NM_001272577.1.
    NP_524877.1. NM_080138.3.
    UniGenei Dm.1772.

    3D structure databases

    ProteinModelPortali P46461.
    SMRi P46461. Positions 5-200, 214-738.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 70577. 11 interactions.
    DIPi DIP-20666N.
    IntActi P46461. 1 interaction.
    MINTi MINT-288692.
    STRINGi 7227.FBpp0073585.

    Proteomic databases

    PaxDbi P46461.
    PRIDEi P46461.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0073754 ; FBpp0073585 ; FBgn0000346 .
    FBtr0333329 ; FBpp0305521 ; FBgn0000346 .
    GeneIDi 47091.
    KEGGi dme:Dmel_CG1618.

    Organism-specific databases

    CTDi 1312.
    FlyBasei FBgn0000346. comt.

    Phylogenomic databases

    eggNOGi COG0464.
    GeneTreei ENSGT00530000064085.
    InParanoidi P46461.
    KOi K06027.
    OMAi ESARQCE.
    OrthoDBi EOG7P8P7F.
    PhylomeDBi P46461.

    Miscellaneous databases

    GenomeRNAii 47091.
    NextBioi 838904.

    Gene expression databases

    Bgeei P46461.

    Family and domain databases

    Gene3Di 3.10.330.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR009010. Asp_de-COase-like_dom.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR004201. Cdc48_dom2.
    IPR029067. CDC48_domain_2-like.
    IPR003338. CDC4_N-term_subdom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00004. AAA. 2 hits.
    PF02933. CDC48_2. 1 hit.
    PF02359. CDC48_N. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 2 hits.
    SM01072. CDC48_2. 1 hit.
    SM01073. CDC48_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF54585. SSF54585. 1 hit.
    PROSITEi PS00674. AAA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Neurally expressed Drosophila genes encoding homologs of the NSF and SNAP secretory proteins."
      Ordway R.W., Pallanck L., Ganetzky B.
      Proc. Natl. Acad. Sci. U.S.A. 91:5715-5719(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Head.
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    3. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Embryo.

    Entry informationi

    Entry nameiNSF1_DROME
    AccessioniPrimary (citable) accession number: P46461
    Secondary accession number(s): Q9VYF4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 139 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3