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Protein

Vesicle-fusing ATPase 1

Gene

comt

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi550 – 5501Magnesium

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi505 – 5106ATPBy similarity
Nucleotide bindingi545 – 5528ATPBy similarity

GO - Molecular functioni

  1. ATPase activity Source: FlyBase
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
  4. protein complex binding Source: FlyBase

GO - Biological processi

  1. Golgi organization Source: FlyBase
  2. Golgi to plasma membrane protein transport Source: GO_Central
  3. Golgi vesicle docking Source: GO_Central
  4. intra-Golgi vesicle-mediated transport Source: GO_Central
  5. metabolic process Source: GOC
  6. neuromuscular synaptic transmission Source: FlyBase
  7. neurotransmitter secretion Source: FlyBase
  8. phagocytosis Source: FlyBase
  9. positive regulation of SNARE complex disassembly Source: FlyBase
  10. protein hexamerization Source: FlyBase
  11. protein homooligomerization Source: FlyBase
  12. regulation of short-term neuronal synaptic plasticity Source: FlyBase
  13. synaptic vesicle fusion to presynaptic membrane Source: FlyBase
  14. synaptic vesicle priming Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_263519. Trafficking of GluR2-containing AMPA receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-fusing ATPase 1 (EC:3.6.4.6)
Alternative name(s):
N-ethylmaleimide-sensitive fusion protein 1
Short name:
NEM-sensitive fusion protein 1
Protein comatose
Vesicular-fusion protein NSF1
dNsf-1
Short name:
NSF-1
Gene namesi
Name:comt
Synonyms:Nsf, Nsf1
ORF Names:CG1618
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome X

Organism-specific databases

FlyBaseiFBgn0000346. comt.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: FlyBase
  2. Golgi stack Source: GO_Central
  3. terminal bouton Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 745745Vesicle-fusing ATPase 1PRO_0000084566Add
BLAST

Proteomic databases

PaxDbiP46461.
PRIDEiP46461.

Expressioni

Tissue specificityi

Nervous system.

Gene expression databases

BgeeiP46461.
ExpressionAtlasiP46461. differential.

Interactioni

Subunit structurei

Homohexamer.By similarity

Protein-protein interaction databases

BioGridi70577. 11 interactions.
DIPiDIP-20666N.
IntActiP46461. 8 interactions.
MINTiMINT-288692.
STRINGi7227.FBpp0073585.

Structurei

3D structure databases

ProteinModelPortaliP46461.
SMRiP46461. Positions 5-200, 214-738.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0464.
GeneTreeiENSGT00530000064085.
InParanoidiP46461.
KOiK06027.
OMAiNDDVDNK.
OrthoDBiEOG7P8P7F.
PhylomeDBiP46461.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
SM01073. CDC48_N. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46461-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYILKATKC PTDELSLTNR AIVNVGDFPE EIKYADISPA PGQHFIFALE
60 70 80 90 100
KTVEVPSGYV GFSLVQRKWA MVSINQELEV RPYRFDASSD VITCVSFETD
110 120 130 140 150
FLQKKTVSQE PYDSDQMAKE FIMQFAGMAL TVGQSLVFNF KDKKLLGLAV
160 170 180 190 200
KSLEAIDPKS LGEGKDTAMR NVRFGRILGN TVVQFEKAEN SSLNLQGKSK
210 220 230 240 250
GKVVRQSIIN PDWDFGKMGI GGLDKEFNSI FRRAFASRVF PPELVEQLGC
260 270 280 290 300
KHVKGILLYG PPGTGKTLMA RQIGTMLNAR EPKIVNGPQI LDKYVGESEA
310 320 330 340 350
NVRRLFAEAE EEEKRLGPNS GLHIIIFDEI DAICKQRGSV AGNSGVHDTV
360 370 380 390 400
VNQLLTKIDG VDQLNNILVI GMTNRRDMID EALLRPGRLE VQMEISLPNE
410 420 430 440 450
QGRVQILNIH TKRMREFNKI NDDVDNKEIA ALTKNFSGAE LEGLVRAAQS
460 470 480 490 500
SAMNRLIKAD AKVTVDPEAM EKLKVNRDDF LHSLEHDIKP AFGTAQEILD
510 520 530 540 550
NMLARGVINW GAPVSNLLED GMLYVQQAKA PESSGLVSVL VAGAPNSGKT
560 570 580 590 600
ALAAQLAKMS DFPFVKVCSP EDMVGYTESA KCLHIRKIFD DAYRSMLSCI
610 620 630 640 650
VVDNVERLLD YGSIGPRYSN MTLQALLVLL KKQPPKGRKL LILCTSSRRE
660 670 680 690 700
VLEEMEMLTA FTSVLHVPNL SKPDHVLAVL ENTDIFSKGE IQAIGKKMAG
710 720 730 740
KRVFIGIKKL LGLIDMARQT EQSQRAIKFL SKMEEEGGLD MVARQ
Length:745
Mass (Da):82,555
Last modified:November 1, 1995 - v1
Checksum:iA34E7B037E91769F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09373 mRNA. Translation: AAA83413.1.
AE014298 Genomic DNA. Translation: AAF48244.2.
BT010259 mRNA. Translation: AAQ23577.1.
RefSeqiNP_001259506.1. NM_001272577.2.
NP_524877.1. NM_080138.4.
UniGeneiDm.1772.

Genome annotation databases

EnsemblMetazoaiFBtr0073754; FBpp0073585; FBgn0000346.
FBtr0333329; FBpp0305521; FBgn0000346.
GeneIDi47091.
KEGGidme:Dmel_CG1618.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09373 mRNA. Translation: AAA83413.1.
AE014298 Genomic DNA. Translation: AAF48244.2.
BT010259 mRNA. Translation: AAQ23577.1.
RefSeqiNP_001259506.1. NM_001272577.2.
NP_524877.1. NM_080138.4.
UniGeneiDm.1772.

3D structure databases

ProteinModelPortaliP46461.
SMRiP46461. Positions 5-200, 214-738.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi70577. 11 interactions.
DIPiDIP-20666N.
IntActiP46461. 8 interactions.
MINTiMINT-288692.
STRINGi7227.FBpp0073585.

Proteomic databases

PaxDbiP46461.
PRIDEiP46461.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0073754; FBpp0073585; FBgn0000346.
FBtr0333329; FBpp0305521; FBgn0000346.
GeneIDi47091.
KEGGidme:Dmel_CG1618.

Organism-specific databases

CTDi1312.
FlyBaseiFBgn0000346. comt.

Phylogenomic databases

eggNOGiCOG0464.
GeneTreeiENSGT00530000064085.
InParanoidiP46461.
KOiK06027.
OMAiNDDVDNK.
OrthoDBiEOG7P8P7F.
PhylomeDBiP46461.

Enzyme and pathway databases

ReactomeiREACT_263519. Trafficking of GluR2-containing AMPA receptors.

Miscellaneous databases

GenomeRNAii47091.
NextBioi838904.

Gene expression databases

BgeeiP46461.
ExpressionAtlasiP46461. differential.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
SM01073. CDC48_N. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Neurally expressed Drosophila genes encoding homologs of the NSF and SNAP secretory proteins."
    Ordway R.W., Pallanck L., Ganetzky B.
    Proc. Natl. Acad. Sci. U.S.A. 91:5715-5719(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Head.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.

Entry informationi

Entry nameiNSF1_DROME
AccessioniPrimary (citable) accession number: P46461
Secondary accession number(s): Q9VYF4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 4, 2015
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.