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P46461 (NSF1_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 135. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vesicle-fusing ATPase 1

EC=3.6.4.6
Alternative name(s):
N-ethylmaleimide-sensitive fusion protein 1
Short name=NEM-sensitive fusion protein 1
Protein comatose
Vesicular-fusion protein NSF1
dNsf-1
Short name=NSF-1
Gene names
Name:comt
Synonyms:Nsf, Nsf1
ORF Names:CG1618
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length745 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.

Catalytic activity

ATP + H2O = ADP + phosphate.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Subunit structure

Homohexamer By similarity.

Subcellular location

Cytoplasm.

Tissue specificity

Nervous system.

Sequence similarities

Belongs to the AAA ATPase family.

Ontologies

Keywords
   Biological processER-Golgi transport
Protein transport
Transport
   Cellular componentCytoplasm
   DomainRepeat
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processATP catabolic process

Inferred from direct assay PubMed 12070132. Source: GOC

Golgi organization

Inferred from mutant phenotype PubMed 11514618. Source: FlyBase

neuromuscular synaptic transmission

Inferred from expression pattern PubMed 21316453. Source: FlyBase

neurotransmitter secretion

Non-traceable author statement PubMed 10798391. Source: FlyBase

phagocytosis

Inferred from mutant phenotype PubMed 16336044. Source: FlyBase

positive regulation of SNARE complex disassembly

Inferred from direct assay PubMed 12070132. Source: FlyBase

protein hexamerization

Inferred from direct assay PubMed 12070132. Source: FlyBase

protein homooligomerization

Inferred from direct assay PubMed 12070132. Source: FlyBase

protein transport

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of short-term neuronal synaptic plasticity

Inferred from direct assay PubMed 19706552. Source: FlyBase

synaptic vesicle fusion to presynaptic membrane

Inferred from mutant phenotype PubMed 21316453. Source: FlyBase

synaptic vesicle priming

Non-traceable author statement PubMed 10798391. Source: FlyBase

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 21316453. Source: FlyBase

terminal bouton

Inferred from direct assay PubMed 21316453. Source: FlyBase

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ATPase activity

Inferred from direct assay PubMed 12070132. Source: FlyBase

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein complex binding

Inferred from physical interaction PubMed 21316453. Source: FlyBase

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 745745Vesicle-fusing ATPase 1
PRO_0000084566

Regions

Nucleotide binding505 – 5106ATP By similarity
Nucleotide binding545 – 5528ATP By similarity

Sites

Metal binding5501Magnesium

Sequences

Sequence LengthMass (Da)Tools
P46461 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: A34E7B037E91769F

FASTA74582,555
        10         20         30         40         50         60 
MAYILKATKC PTDELSLTNR AIVNVGDFPE EIKYADISPA PGQHFIFALE KTVEVPSGYV 

        70         80         90        100        110        120 
GFSLVQRKWA MVSINQELEV RPYRFDASSD VITCVSFETD FLQKKTVSQE PYDSDQMAKE 

       130        140        150        160        170        180 
FIMQFAGMAL TVGQSLVFNF KDKKLLGLAV KSLEAIDPKS LGEGKDTAMR NVRFGRILGN 

       190        200        210        220        230        240 
TVVQFEKAEN SSLNLQGKSK GKVVRQSIIN PDWDFGKMGI GGLDKEFNSI FRRAFASRVF 

       250        260        270        280        290        300 
PPELVEQLGC KHVKGILLYG PPGTGKTLMA RQIGTMLNAR EPKIVNGPQI LDKYVGESEA 

       310        320        330        340        350        360 
NVRRLFAEAE EEEKRLGPNS GLHIIIFDEI DAICKQRGSV AGNSGVHDTV VNQLLTKIDG 

       370        380        390        400        410        420 
VDQLNNILVI GMTNRRDMID EALLRPGRLE VQMEISLPNE QGRVQILNIH TKRMREFNKI 

       430        440        450        460        470        480 
NDDVDNKEIA ALTKNFSGAE LEGLVRAAQS SAMNRLIKAD AKVTVDPEAM EKLKVNRDDF 

       490        500        510        520        530        540 
LHSLEHDIKP AFGTAQEILD NMLARGVINW GAPVSNLLED GMLYVQQAKA PESSGLVSVL 

       550        560        570        580        590        600 
VAGAPNSGKT ALAAQLAKMS DFPFVKVCSP EDMVGYTESA KCLHIRKIFD DAYRSMLSCI 

       610        620        630        640        650        660 
VVDNVERLLD YGSIGPRYSN MTLQALLVLL KKQPPKGRKL LILCTSSRRE VLEEMEMLTA 

       670        680        690        700        710        720 
FTSVLHVPNL SKPDHVLAVL ENTDIFSKGE IQAIGKKMAG KRVFIGIKKL LGLIDMARQT 

       730        740 
EQSQRAIKFL SKMEEEGGLD MVARQ 

« Hide

References

« Hide 'large scale' references
[1]"Neurally expressed Drosophila genes encoding homologs of the NSF and SNAP secretory proteins."
Ordway R.W., Pallanck L., Ganetzky B.
Proc. Natl. Acad. Sci. U.S.A. 91:5715-5719(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Head.
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: Berkeley.
[4]Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. expand/collapse author list , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.
Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Berkeley.
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U09373 mRNA. Translation: AAA83413.1.
AE014298 Genomic DNA. Translation: AAF48244.2.
BT010259 mRNA. Translation: AAQ23577.1.
RefSeqNP_001259506.1. NM_001272577.1.
NP_524877.1. NM_080138.3.
UniGeneDm.1772.

3D structure databases

ProteinModelPortalP46461.
SMRP46461. Positions 4-738.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid70577. 11 interactions.
DIPDIP-20666N.
IntActP46461. 1 interaction.
MINTMINT-288692.
STRING7227.FBpp0073585.

Proteomic databases

PaxDbP46461.
PRIDEP46461.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0073754; FBpp0073585; FBgn0000346.
FBtr0333329; FBpp0305521; FBgn0000346.
GeneID47091.
KEGGdme:Dmel_CG1618.

Organism-specific databases

CTD1312.
FlyBaseFBgn0000346. comt.

Phylogenomic databases

eggNOGCOG0464.
GeneTreeENSGT00530000064085.
InParanoidP46461.
KOK06027.
OMAVQQAKAP.
OrthoDBEOG7P8P7F.
PhylomeDBP46461.

Gene expression databases

BgeeP46461.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
SM01073. CDC48_N. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi47091.
NextBio838904.

Entry information

Entry nameNSF1_DROME
AccessionPrimary (citable) accession number: P46461
Secondary accession number(s): Q9VYF4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase