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Protein

Vesicle-fusing ATPase

Gene

Nsf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack GRIA2 leads to influence GRIA2 membrane cycling (By similarity).By similarity1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi550MagnesiumBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi505 – 510ATPBy similarity6
Nucleotide bindingi545 – 552ATPBy similarity8

GO - Molecular functioni

  • ATPase activity Source: ParkinsonsUK-UCL
  • ATPase activity, coupled Source: MGI
  • ATP binding Source: UniProtKB-KW
  • ionotropic glutamate receptor binding Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • PDZ domain binding Source: MGI
  • protein-containing complex binding Source: ParkinsonsUK-UCL
  • protein kinase binding Source: ParkinsonsUK-UCL
  • SNARE binding Source: ParkinsonsUK-UCL
  • syntaxin-1 binding Source: ParkinsonsUK-UCL
  • syntaxin binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processProtein transport, Transport
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-204005 COPII-mediated vesicle transport
R-MMU-416993 Trafficking of GluR2-containing AMPA receptors
R-MMU-6807878 COPI-mediated anterograde transport
R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-MMU-6811438 Intra-Golgi traffic
R-MMU-6811440 Retrograde transport at the Trans-Golgi-Network

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-fusing ATPase (EC:3.6.4.6)
Alternative name(s):
N-ethylmaleimide-sensitive fusion protein
Short name:
NEM-sensitive fusion protein
Suppressor of K(+) transport growth defect 2
Short name:
Protein SKD2
Vesicular-fusion protein NSF
Gene namesi
Name:Nsf
Synonyms:Skd2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:104560 Nsf

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi569S → A: Abolishes phosphorylation by CDK16. 1 Publication1
Mutagenesisi569S → E: Interferes with oligomerization. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000845641 – 744Vesicle-fusing ATPaseAdd BLAST744

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei105N6-acetyllysineCombined sources1
Modified residuei207PhosphoserineCombined sources1
Modified residuei259PhosphotyrosineCombined sources1
Modified residuei569Phosphoserine; by CDK161 Publication1

Post-translational modificationi

Phosphorylation at Ser-569 interferes with homohexamerization.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP46460
MaxQBiP46460
PaxDbiP46460
PeptideAtlasiP46460
PRIDEiP46460

2D gel databases

REPRODUCTION-2DPAGEiP46460

PTM databases

iPTMnetiP46460
PhosphoSitePlusiP46460
SwissPalmiP46460

Expressioni

Gene expression databases

BgeeiENSMUSG00000034187
CleanExiMM_NSF
ExpressionAtlasiP46460 baseline and differential
GenevisibleiP46460 MM

Interactioni

Subunit structurei

Homohexamer. Interacts with GABARAP and GABARAPL2. Interacts with GRIA2. Interacts with PLK2, leading to disrupt the interaction with GRIA2. Interacts with MUSK; may regulate MUSK endocytosis and activity (By similarity). Interacts with CDK16.By similarity2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • ionotropic glutamate receptor binding Source: MGI
  • PDZ domain binding Source: MGI
  • protein kinase binding Source: ParkinsonsUK-UCL
  • SNARE binding Source: ParkinsonsUK-UCL
  • syntaxin-1 binding Source: ParkinsonsUK-UCL
  • syntaxin binding Source: MGI

Protein-protein interaction databases

BioGridi201857, 13 interactors
IntActiP46460, 26 interactors
MINTiP46460
STRINGi10090.ENSMUSP00000099364

Structurei

3D structure databases

ProteinModelPortaliP46460
SMRiP46460
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0741 Eukaryota
COG0464 LUCA
GeneTreeiENSGT00530000064085
HOVERGENiHBG000324
InParanoidiP46460
KOiK06027
OMAiIDWVPIG
OrthoDBiEOG091G02LU
PhylomeDBiP46460
TreeFamiTF300371

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR009010 Asp_de-COase-like_dom_sf
IPR003959 ATPase_AAA_core
IPR003960 ATPase_AAA_CS
IPR004201 Cdc48_dom2
IPR029067 CDC48_domain_2-like_sf
IPR003338 CDC4_N-term_subdom
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00004 AAA, 2 hits
PF02933 CDC48_2, 1 hit
PF02359 CDC48_N, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SM01072 CDC48_2, 1 hit
SM01073 CDC48_N, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
SSF52540 SSF52540, 2 hits
SSF54585 SSF54585, 1 hit
PROSITEiView protein in PROSITE
PS00674 AAA, 1 hit

Sequencei

Sequence statusi: Complete.

P46460-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGRTMQAAR CPTDELSLSN CAVVNEKDFQ SGQHVMVRTS PNHKYIFTLR
60 70 80 90 100
THPSVVPGCI AFSLPQRKWA GLSIGQDIEV ALYSFDKAKQ CIGTMTIEID
110 120 130 140 150
FLQKKNIDSN PYDTDKMAAE FIQQFNNQAF SVGQQLVFSF NDKLFGLLVK
160 170 180 190 200
DIEAMDPSIL KGEPASGKRQ KIEVGLVVGN SQVAFEKAEN SSLNLIGKAK
210 220 230 240 250
TKENRQSIIN PDWNFEKMGI GGLDKEFSDI FRRAFASRVF PPEIVEQMGC
260 270 280 290 300
KHVKGILLYG PPGCGKTLLA RQIGKMLNAR EPKVVNGPEI LNKYVGESEA
310 320 330 340 350
NIRKLFADAE EEQRRLGANS GLHIIIFDEI DAICKQRGSM AGSTGVHDTV
360 370 380 390 400
VNQLLSKIDG VEQLNNILVI GMTNRPDLID EALLRPGRLE VKMEIGLPDE
410 420 430 440 450
KGRLQILHIH TARMRGHQLL SADVDIKELA VETKNFSGAE LEGLVRAAQS
460 470 480 490 500
TAMNRHIKAS TKVEVDMEKA ESLQVTRGDF LASLENDIKP AFGTNQEDYA
510 520 530 540 550
SYIMNGIIKW GDPVTRVLDD GELLVQQTKN SDRTPLVSVL LEGPPHSGKT
560 570 580 590 600
ALAAKIAEES NFPFIKICSP DKMIGFSETA KCQAMKKIFD DAYKSQLSCV
610 620 630 640 650
VVDDIERLLD YVPIGPRFSN LVLQALLVLL KKAPPQGRKL LIIGTTSRKD
660 670 680 690 700
VLQEMEMLNA FSTTIHVPNI ATGEQLLEAL ELLGNFKDKE RTTIAQQVKG
710 720 730 740
KKVWIGIKKL LMLIEMSLQM DPEYRVRKFL ALMREEGASP LDFD
Length:744
Mass (Da):82,613
Last modified:May 1, 2007 - v2
Checksum:iBE55AD0056AD5585
GO

Sequence cautioni

The sequence CAM20943 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAM23742 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti300A → G in BAC39361 (PubMed:16141072).Curated1
Sequence conflicti316 – 326LGANSGLHIII → VCGHKTNLLKM in BAC34637 (PubMed:16141072).CuratedAdd BLAST11
Sequence conflicti492F → I in AAA50498 (PubMed:8082782).Curated1
Sequence conflicti552L → V in AAA50498 (PubMed:8082782).Curated1
Sequence conflicti657M → I in BAC25937 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10120 mRNA Translation: AAA50498.1
AK028415 mRNA Translation: BAC25937.1
AK032120 mRNA Translation: BAC27713.1
AK049281 mRNA Translation: BAC33656.1
AK051430 mRNA Translation: BAC34637.1
AK085086 mRNA Translation: BAC39361.1
AK153905 mRNA Translation: BAE32247.1
AL596108, AL603829 Genomic DNA Translation: CAM20943.1 Sequence problems.
AL596108, AL603829 Genomic DNA Translation: CAM20945.1
AL603829, AL596108 Genomic DNA Translation: CAM23742.1 Sequence problems.
AL603829, AL596108 Genomic DNA Translation: CAM23743.1
BC006627 mRNA Translation: AAH06627.1
BC019167 mRNA Translation: AAH19167.1
CCDSiCCDS25524.1
RefSeqiNP_032766.2, NM_008740.4
UniGeneiMm.260117
Mm.474887

Genome annotation databases

EnsembliENSMUST00000103075; ENSMUSP00000099364; ENSMUSG00000034187
GeneIDi18195
KEGGimmu:18195
UCSCiuc007lvt.1 mouse

Similar proteinsi

Entry informationi

Entry nameiNSF_MOUSE
AccessioniPrimary (citable) accession number: P46460
Secondary accession number(s): A2A646
, Q8BQ65, Q8C3R2, Q8CCT9, Q8CEF0, Q923C6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: May 1, 2007
Last modified: May 23, 2018
This is version 170 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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