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P46460

- NSF_MOUSE

UniProt

P46460 - NSF_MOUSE

Protein

Vesicle-fusing ATPase

Gene

Nsf

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 2 (01 May 2007)
      Previous versions | rss
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    Functioni

    Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack GRIA2 leads to influence GRIA2 membrane cycling By similarity.By similarity

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi550 – 5501Magnesium

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi505 – 5106ATPBy similarity
    Nucleotide bindingi545 – 5528ATPBy similarity

    GO - Molecular functioni

    1. ATPase activity, coupled Source: MGI
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW
    4. protein binding Source: IntAct
    5. protein kinase binding Source: ParkinsonsUK-UCL
    6. syntaxin-1 binding Source: ParkinsonsUK-UCL
    7. syntaxin binding Source: MGI

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. positive regulation of receptor recycling Source: Ensembl
    3. potassium ion transport Source: MGI
    4. protein transport Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Protein transport, Transport

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vesicle-fusing ATPase (EC:3.6.4.6)
    Alternative name(s):
    N-ethylmaleimide-sensitive fusion protein
    Short name:
    NEM-sensitive fusion protein
    Suppressor of K(+) transport growth defect 2
    Short name:
    Protein SKD2
    Vesicular-fusion protein NSF
    Gene namesi
    Name:Nsf
    Synonyms:Skd2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:104560. Nsf.

    Subcellular locationi

    GO - Cellular componenti

    1. lysosomal membrane Source: Ensembl
    2. plasma membrane Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi569 – 5691S → A: Abolishes phosphorylation by CDK16. 1 Publication
    Mutagenesisi569 – 5691S → E: Interferes with oligomerization. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 744744Vesicle-fusing ATPasePRO_0000084564Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei105 – 1051N6-acetyllysine1 Publication
    Modified residuei259 – 2591Phosphotyrosine1 Publication
    Modified residuei569 – 5691Phosphoserine; by CDK161 Publication

    Post-translational modificationi

    Phosphorylation at Ser-569 interferes with homohexamerization.2 Publications

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP46460.
    PaxDbiP46460.
    PRIDEiP46460.

    2D gel databases

    REPRODUCTION-2DPAGEP46460.

    PTM databases

    PhosphoSiteiP46460.

    Expressioni

    Gene expression databases

    ArrayExpressiP46460.
    BgeeiP46460.
    CleanExiMM_NSF.
    GenevestigatoriP46460.

    Interactioni

    Subunit structurei

    Homohexamer. Interacts with GABARAP and GABARAPL2. Interacts with GRIA2. Interacts with PLK2, leading to disrupt the interaction with GRIA2. Interacts with MUSK; may regulate MUSK endocytosis and activity By similarity. Interacts with CDK16.By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Dlg4Q621084EBI-398006,EBI-300895
    Lrrk2Q5S0063EBI-398006,EBI-2693710
    MuskQ61006-35EBI-398006,EBI-6308424
    Ptpn9O352393EBI-398006,EBI-7297868

    Protein-protein interaction databases

    BioGridi201857. 5 interactions.
    IntActiP46460. 19 interactions.
    MINTiMINT-89339.

    Structurei

    3D structure databases

    ProteinModelPortaliP46460.
    SMRiP46460. Positions 1-201, 220-742.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AAA ATPase family.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0464.
    GeneTreeiENSGT00530000064085.
    HOVERGENiHBG000324.
    InParanoidiP46460.
    KOiK06027.
    OMAiFKDKERS.
    OrthoDBiEOG7P8P7F.
    PhylomeDBiP46460.
    TreeFamiTF300371.

    Family and domain databases

    Gene3Di3.10.330.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR009010. Asp_de-COase-like_dom.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR004201. Cdc48_dom2.
    IPR029067. CDC48_domain_2-like.
    IPR003338. CDC4_N-term_subdom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00004. AAA. 2 hits.
    PF02933. CDC48_2. 1 hit.
    PF02359. CDC48_N. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 2 hits.
    SM01072. CDC48_2. 1 hit.
    SM01073. CDC48_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF54585. SSF54585. 1 hit.
    PROSITEiPS00674. AAA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P46460-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGRTMQAAR CPTDELSLSN CAVVNEKDFQ SGQHVMVRTS PNHKYIFTLR    50
    THPSVVPGCI AFSLPQRKWA GLSIGQDIEV ALYSFDKAKQ CIGTMTIEID 100
    FLQKKNIDSN PYDTDKMAAE FIQQFNNQAF SVGQQLVFSF NDKLFGLLVK 150
    DIEAMDPSIL KGEPASGKRQ KIEVGLVVGN SQVAFEKAEN SSLNLIGKAK 200
    TKENRQSIIN PDWNFEKMGI GGLDKEFSDI FRRAFASRVF PPEIVEQMGC 250
    KHVKGILLYG PPGCGKTLLA RQIGKMLNAR EPKVVNGPEI LNKYVGESEA 300
    NIRKLFADAE EEQRRLGANS GLHIIIFDEI DAICKQRGSM AGSTGVHDTV 350
    VNQLLSKIDG VEQLNNILVI GMTNRPDLID EALLRPGRLE VKMEIGLPDE 400
    KGRLQILHIH TARMRGHQLL SADVDIKELA VETKNFSGAE LEGLVRAAQS 450
    TAMNRHIKAS TKVEVDMEKA ESLQVTRGDF LASLENDIKP AFGTNQEDYA 500
    SYIMNGIIKW GDPVTRVLDD GELLVQQTKN SDRTPLVSVL LEGPPHSGKT 550
    ALAAKIAEES NFPFIKICSP DKMIGFSETA KCQAMKKIFD DAYKSQLSCV 600
    VVDDIERLLD YVPIGPRFSN LVLQALLVLL KKAPPQGRKL LIIGTTSRKD 650
    VLQEMEMLNA FSTTIHVPNI ATGEQLLEAL ELLGNFKDKE RTTIAQQVKG 700
    KKVWIGIKKL LMLIEMSLQM DPEYRVRKFL ALMREEGASP LDFD 744
    Length:744
    Mass (Da):82,613
    Last modified:May 1, 2007 - v2
    Checksum:iBE55AD0056AD5585
    GO

    Sequence cautioni

    The sequence CAM20943.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAM23742.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti300 – 3001A → G in BAC39361. (PubMed:16141072)Curated
    Sequence conflicti316 – 32611LGANSGLHIII → VCGHKTNLLKM in BAC34637. (PubMed:16141072)CuratedAdd
    BLAST
    Sequence conflicti492 – 4921F → I in AAA50498. (PubMed:8082782)Curated
    Sequence conflicti552 – 5521L → V in AAA50498. (PubMed:8082782)Curated
    Sequence conflicti657 – 6571M → I in BAC25937. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10120 mRNA. Translation: AAA50498.1.
    AK028415 mRNA. Translation: BAC25937.1.
    AK032120 mRNA. Translation: BAC27713.1.
    AK049281 mRNA. Translation: BAC33656.1.
    AK051430 mRNA. Translation: BAC34637.1.
    AK085086 mRNA. Translation: BAC39361.1.
    AK153905 mRNA. Translation: BAE32247.1.
    AL596108, AL603829 Genomic DNA. Translation: CAM20943.1. Sequence problems.
    AL596108, AL603829 Genomic DNA. Translation: CAM20945.1.
    AL603829, AL596108 Genomic DNA. Translation: CAM23742.1. Sequence problems.
    AL603829, AL596108 Genomic DNA. Translation: CAM23743.1.
    BC006627 mRNA. Translation: AAH06627.1.
    BC019167 mRNA. Translation: AAH19167.1.
    CCDSiCCDS25524.1.
    RefSeqiNP_032766.2. NM_008740.4.
    UniGeneiMm.260117.
    Mm.474887.

    Genome annotation databases

    EnsembliENSMUST00000103075; ENSMUSP00000099364; ENSMUSG00000034187.
    GeneIDi18195.
    KEGGimmu:18195.
    UCSCiuc007lvt.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10120 mRNA. Translation: AAA50498.1 .
    AK028415 mRNA. Translation: BAC25937.1 .
    AK032120 mRNA. Translation: BAC27713.1 .
    AK049281 mRNA. Translation: BAC33656.1 .
    AK051430 mRNA. Translation: BAC34637.1 .
    AK085086 mRNA. Translation: BAC39361.1 .
    AK153905 mRNA. Translation: BAE32247.1 .
    AL596108 , AL603829 Genomic DNA. Translation: CAM20943.1 . Sequence problems.
    AL596108 , AL603829 Genomic DNA. Translation: CAM20945.1 .
    AL603829 , AL596108 Genomic DNA. Translation: CAM23742.1 . Sequence problems.
    AL603829 , AL596108 Genomic DNA. Translation: CAM23743.1 .
    BC006627 mRNA. Translation: AAH06627.1 .
    BC019167 mRNA. Translation: AAH19167.1 .
    CCDSi CCDS25524.1.
    RefSeqi NP_032766.2. NM_008740.4.
    UniGenei Mm.260117.
    Mm.474887.

    3D structure databases

    ProteinModelPortali P46460.
    SMRi P46460. Positions 1-201, 220-742.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201857. 5 interactions.
    IntActi P46460. 19 interactions.
    MINTi MINT-89339.

    PTM databases

    PhosphoSitei P46460.

    2D gel databases

    REPRODUCTION-2DPAGE P46460.

    Proteomic databases

    MaxQBi P46460.
    PaxDbi P46460.
    PRIDEi P46460.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000103075 ; ENSMUSP00000099364 ; ENSMUSG00000034187 .
    GeneIDi 18195.
    KEGGi mmu:18195.
    UCSCi uc007lvt.1. mouse.

    Organism-specific databases

    CTDi 4905.
    MGIi MGI:104560. Nsf.

    Phylogenomic databases

    eggNOGi COG0464.
    GeneTreei ENSGT00530000064085.
    HOVERGENi HBG000324.
    InParanoidi P46460.
    KOi K06027.
    OMAi FKDKERS.
    OrthoDBi EOG7P8P7F.
    PhylomeDBi P46460.
    TreeFami TF300371.

    Miscellaneous databases

    ChiTaRSi NSF. mouse.
    NextBioi 293548.
    PROi P46460.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P46460.
    Bgeei P46460.
    CleanExi MM_NSF.
    Genevestigatori P46460.

    Family and domain databases

    Gene3Di 3.10.330.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR009010. Asp_de-COase-like_dom.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR004201. Cdc48_dom2.
    IPR029067. CDC48_domain_2-like.
    IPR003338. CDC4_N-term_subdom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00004. AAA. 2 hits.
    PF02933. CDC48_2. 1 hit.
    PF02359. CDC48_N. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 2 hits.
    SM01072. CDC48_2. 1 hit.
    SM01073. CDC48_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF54585. SSF54585. 1 hit.
    PROSITEi PS00674. AAA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a novel mammalian member of the NSF/CDC48p/Pas1p/TBP-1 family through heterologous expression in yeast."
      Perier F., Coulter K.L., Liang H., Radeke C.M., Gaber R.F., Vandenberg C.A.
      FEBS Lett. 351:286-290(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Head, Lung, Medulla oblongata, Spinal ganglion and Thymus.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Eye and Mammary tumor.
    5. Lubec G., Kang S.U., Klug S., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 45-50; 69-87; 106-116; 151-161; 170-198; 218-232; 255-266; 294-303; 305-314; 316-335; 338-357; 404-413; 416-427; 435-446; 517-529; 534-559; 573-581; 595-631; 640-648 AND 710-725, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6 and OF1.
      Tissue: Brain and Hippocampus.
    6. "Pctaire1 phosphorylates N-ethylmaleimide-sensitive fusion protein: implications in the regulation of its hexamerization and exocytosis."
      Liu Y., Cheng K., Gong K., Fu A.K., Ip N.Y.
      J. Biol. Chem. 281:9852-9858(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CDK16, SUBUNIT, MUTAGENESIS OF SER-569, PHOSPHORYLATION AT SER-569.
    7. "Muscle-specific receptor tyrosine kinase endocytosis in acetylcholine receptor clustering in response to agrin."
      Zhu D., Yang Z., Luo Z., Luo S., Xiong W.C., Mei L.
      J. Neurosci. 28:1688-1696(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MUSK.
    8. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
      Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
      J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-259, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    9. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-105, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiNSF_MOUSE
    AccessioniPrimary (citable) accession number: P46460
    Secondary accession number(s): A2A646
    , Q8BQ65, Q8C3R2, Q8CCT9, Q8CEF0, Q923C6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: May 1, 2007
    Last modified: October 1, 2014
    This is version 136 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3