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P46459

- NSF_HUMAN

UniProt

P46459 - NSF_HUMAN

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Protein
Vesicle-fusing ATPase
Gene
NSF
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling By similarity.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Cofactori

Binds 1 magnesium ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi550 – 5501Magnesium By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi505 – 5106ATP By similarity
Nucleotide bindingi545 – 5528ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATPase activity, coupled Source: Ensembl
  3. PDZ domain binding Source: UniProtKB
  4. metal ion binding Source: UniProtKB-KW
  5. protein binding Source: UniProtKB
  6. protein complex binding Source: UniProtKB
  7. syntaxin-1 binding Source: ParkinsonsUK-UCL

GO - Biological processi

  1. exocytosis Source: UniProtKB
  2. plasma membrane fusion Source: UniProtKB
  3. positive regulation of receptor recycling Source: UniProtKB
  4. potassium ion transport Source: Ensembl
  5. protein transport Source: UniProtKB-KW
  6. synaptic transmission Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_18422. Trafficking of GluR2-containing AMPA receptors.

Protein family/group databases

TCDBi1.F.1.1.1. the synaptosomal vesicle fusion pore (svf-pore) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-fusing ATPase (EC:3.6.4.6)
Alternative name(s):
N-ethylmaleimide-sensitive fusion protein
Short name:
NEM-sensitive fusion protein
Vesicular-fusion protein NSF
Gene namesi
Name:NSF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:8016. NSF.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: HPA
  2. cytosol Source: Reactome
  3. extracellular vesicular exosome Source: UniProt
  4. lysosomal membrane Source: UniProtKB
  5. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA31793.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 744744Vesicle-fusing ATPase
PRO_0000084563Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei105 – 1051N6-acetyllysine By similarity
Modified residuei259 – 2591Phosphotyrosine By similarity
Modified residuei569 – 5691Phosphoserine; by CDK16 By similarity

Post-translational modificationi

Phosphorylation at Ser-569 interferes with homohexamerization By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP46459.
PaxDbiP46459.
PRIDEiP46459.

2D gel databases

UCD-2DPAGEP46459.

PTM databases

PhosphoSiteiP46459.

Miscellaneous databases

PMAP-CutDBP46459.

Expressioni

Gene expression databases

ArrayExpressiP46459.
BgeeiP46459.
CleanExiHS_NSF.
GenevestigatoriP46459.

Organism-specific databases

HPAiCAB009324.
CAB013645.
HPA003154.

Interactioni

Subunit structurei

Homohexamer. Interacts with GABARAP and GABARAPL2. Interacts with GRIA2. Interacts with PLK2, leading to disrupt the interaction with GRIA2. Interacts with MUSK; may regulate MUSK endocytosis and activity By similarity. Interacts with CDK16 By similarity.

Binary interactionsi

WithEntry#Exp.IntActNotes
GABBR1Q9UBS53EBI-712251,EBI-724156
GABBR2O758994EBI-712251,EBI-715469
GRIA2P422622EBI-712251,EBI-3909876
NAPAP549202EBI-712251,EBI-749652
PTPN9P433782EBI-712251,EBI-742898

Protein-protein interaction databases

BioGridi110960. 38 interactions.
DIPiDIP-389N.
IntActiP46459. 37 interactions.
MINTiMINT-1369243.
STRINGi9606.ENSP00000381293.

Structurei

3D structure databases

ProteinModelPortaliP46459.
SMRiP46459. Positions 1-201, 220-742.

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0464.
HOGENOMiHOG000198544.
HOVERGENiHBG000324.
InParanoidiP46459.
KOiK06027.
OMAiFKDKERS.
OrthoDBiEOG7P8P7F.
PhylomeDBiP46459.
TreeFamiTF300371.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
SM01073. CDC48_N. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46459-1 [UniParc]FASTAAdd to Basket

« Hide

MAGRSMQAAR CPTDELSLTN CAVVNEKDFQ SGQHVIVRTS PNHRYTFTLK    50
THPSVVPGSI AFSLPQRKWA GLSIGQEIEV SLYTFDKAKQ CIGTMTIEID 100
FLQKKSIDSN PYDTDKMAAE FIQQFNNQAF SVGQQLVFSF NEKLFGLLVK 150
DIEAMDPSIL KGEPATGKRQ KIEVGLVVGN SQVAFEKAEN SSLNLIGKAK 200
TKENRQSIIN PDWNFEKMGI GGLDKEFSDI FRRAFASRVF PPEIVEQMGC 250
KHVKGILLYG PPGCGKTLLA RQIGKMLNAR EPKVVNGPEI LNKYVGESEA 300
NIRKLFADAE EEQRRLGANS GLHIIIFDEI DAICKQRGSM AGSTGVHDTV 350
VNQLLSKIDG VEQLNNILVI GMTNRPDLID EALLRPGRLE VKMEIGLPDE 400
KGRLQILHIH TARMRGHQLL SADVDIKELA VETKNFSGAE LEGLVRAAQS 450
TAMNRHIKAS TKVEVDMEKA ESLQVTRGDF LASLENDIKP AFGTNQEDYA 500
SYIMNGIIKW GDPVTRVLDD GELLVQQTKN SDRTPLVSVL LEGPPHSGKT 550
ALAAKIAEES NFPFIKICSP DKMIGFSETA KCQAMKKIFD DAYKSQLSCV 600
VVDDIERLLD YVPIGPRFSN LVLQALLVLL KKAPPQGRKL LIIGTTSRKD 650
VLQEMEMLNA FSTTIHVPNI ATGEQLLEAL ELLGNFKDKE RTTIAQQVKG 700
KKVWIGIKKL LMLIEMSLQM DPEYRVRKFL ALLREEGASP LDFD 744
Length:744
Mass (Da):82,594
Last modified:September 1, 2009 - v3
Checksum:iBF545A2A0C7EC2F7
GO

Sequence cautioni

The sequence AAA17411.1 differs from that shown. Reason: Erroneous initiation.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti476 – 4761T → M.
Corresponds to variant rs155733 [ dbSNP | Ensembl ].
VAR_029580

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti22 – 221A → S in AAA17411. 1 Publication
Sequence conflicti22 – 221A → S in AAF70545. 1 Publication
Sequence conflicti25 – 251N → S in BAF82893. 1 Publication
Sequence conflicti107 – 1071I → N in AAA17411. 1 Publication
Sequence conflicti107 – 1071I → N in AAF70545. 1 Publication
Sequence conflicti107 – 1071I → N in AAF04745. 1 Publication
Sequence conflicti130 – 1301F → Y in AAA17411. 1 Publication
Sequence conflicti130 – 1301F → Y in AAF70545. 1 Publication
Sequence conflicti130 – 1301F → Y in AAF04745. 1 Publication
Sequence conflicti154 – 1541A → S in AAA17411. 1 Publication
Sequence conflicti154 – 1541A → S in AAF70545. 1 Publication
Sequence conflicti154 – 1541A → S in AAF04745. 1 Publication
Sequence conflicti237 – 2371S → F in AAA17411. 1 Publication
Sequence conflicti237 – 2371S → F in AAF70545. 1 Publication
Sequence conflicti237 – 2371S → F in AAF04745. 1 Publication
Sequence conflicti251 – 2511K → I in AAA17411. 1 Publication
Sequence conflicti251 – 2511K → I in AAF70545. 1 Publication
Sequence conflicti251 – 2511K → I in AAF04745. 1 Publication
Sequence conflicti427 – 4271K → R in BAF82893. 1 Publication
Sequence conflicti571 – 5711D → E in AAF04745. 1 Publication
Sequence conflicti639 – 6391K → M in BAF82893. 1 Publication
Sequence conflicti686 – 6861F → L in AAA17411. 1 Publication
Sequence conflicti686 – 6861F → L in AAF70545. 1 Publication
Sequence conflicti686 – 6861F → L in AAF04745. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U03985 mRNA. Translation: AAA17411.1. Different initiation.
AF135168 mRNA. Translation: AAF70545.1.
AF102846 mRNA. Translation: AAF04745.2.
AK290204 mRNA. Translation: BAF82893.1.
AC004098 Genomic DNA. No translation available.
AC138645 Genomic DNA. No translation available.
AC138688 Genomic DNA. No translation available.
AC217769 Genomic DNA. No translation available.
AC217778 Genomic DNA. No translation available.
AC217780 Genomic DNA. No translation available.
BC030613 mRNA. Translation: AAH30613.1.
CCDSiCCDS42354.1.
PIRiG01234.
RefSeqiNP_006169.2. NM_006178.3.
UniGeneiHs.431279.

Genome annotation databases

EnsembliENST00000398238; ENSP00000381293; ENSG00000073969.
GeneIDi4905.
KEGGihsa:4905.
UCSCiuc002iku.3. human.

Polymorphism databases

DMDMi257051048.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U03985 mRNA. Translation: AAA17411.1 . Different initiation.
AF135168 mRNA. Translation: AAF70545.1 .
AF102846 mRNA. Translation: AAF04745.2 .
AK290204 mRNA. Translation: BAF82893.1 .
AC004098 Genomic DNA. No translation available.
AC138645 Genomic DNA. No translation available.
AC138688 Genomic DNA. No translation available.
AC217769 Genomic DNA. No translation available.
AC217778 Genomic DNA. No translation available.
AC217780 Genomic DNA. No translation available.
BC030613 mRNA. Translation: AAH30613.1 .
CCDSi CCDS42354.1.
PIRi G01234.
RefSeqi NP_006169.2. NM_006178.3.
UniGenei Hs.431279.

3D structure databases

ProteinModelPortali P46459.
SMRi P46459. Positions 1-201, 220-742.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 110960. 38 interactions.
DIPi DIP-389N.
IntActi P46459. 37 interactions.
MINTi MINT-1369243.
STRINGi 9606.ENSP00000381293.

Chemistry

ChEMBLi CHEMBL2311231.

Protein family/group databases

TCDBi 1.F.1.1.1. the synaptosomal vesicle fusion pore (svf-pore) family.

PTM databases

PhosphoSitei P46459.

Polymorphism databases

DMDMi 257051048.

2D gel databases

UCD-2DPAGE P46459.

Proteomic databases

MaxQBi P46459.
PaxDbi P46459.
PRIDEi P46459.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000398238 ; ENSP00000381293 ; ENSG00000073969 .
GeneIDi 4905.
KEGGi hsa:4905.
UCSCi uc002iku.3. human.

Organism-specific databases

CTDi 4905.
GeneCardsi GC17P044668.
H-InvDB HIX0013914.
HIX0013915.
HGNCi HGNC:8016. NSF.
HPAi CAB009324.
CAB013645.
HPA003154.
MIMi 601633. gene.
neXtProti NX_P46459.
PharmGKBi PA31793.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0464.
HOGENOMi HOG000198544.
HOVERGENi HBG000324.
InParanoidi P46459.
KOi K06027.
OMAi FKDKERS.
OrthoDBi EOG7P8P7F.
PhylomeDBi P46459.
TreeFami TF300371.

Enzyme and pathway databases

Reactomei REACT_18422. Trafficking of GluR2-containing AMPA receptors.

Miscellaneous databases

ChiTaRSi NSF. human.
GeneWikii N-ethylmaleimide_sensitive_fusion_protein.
GenomeRNAii 4905.
NextBioi 18875.
PMAP-CutDB P46459.
PROi P46459.
SOURCEi Search...

Gene expression databases

ArrayExpressi P46459.
Bgeei P46459.
CleanExi HS_NSF.
Genevestigatori P46459.

Family and domain databases

Gene3Di 3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
SM01073. CDC48_N. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEi PS00674. AAA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and localization of a brain N-ethylmaleimide-sensitive factor involved in vesicular transport."
    Hong R., Moriyama Y., Mori H., Futai M., Yamamoto A., Tashiro Y., Fukui T., Tagaya M.
    Submitted (DEC-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. Bui T.D., Lu L., Hong W.
    Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The regulation of hNSF gene expression."
    Zhang R., Liu Z., Wu M.
    Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Thalamus.
  5. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNSF_HUMAN
AccessioniPrimary (citable) accession number: P46459
Secondary accession number(s): A8K2D9, Q8N6D7, Q9UKZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: September 1, 2009
Last modified: September 3, 2014
This is version 144 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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