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Protein

Vesicle-fusing ATPase

Gene

NSF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi550 – 5501MagnesiumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi505 – 5106ATPBy similarity
Nucleotide bindingi545 – 5528ATPBy similarity

GO - Molecular functioni

  1. ATPase activity Source: ParkinsonsUK-UCL
  2. ATPase activity, coupled Source: Ensembl
  3. ATP binding Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW
  5. PDZ domain binding Source: UniProtKB
  6. protein complex binding Source: UniProtKB
  7. protein kinase binding Source: ParkinsonsUK-UCL
  8. Rab GTPase binding Source: ParkinsonsUK-UCL
  9. syntaxin-1 binding Source: ParkinsonsUK-UCL

GO - Biological processi

  1. exocytosis Source: UniProtKB
  2. Golgi to plasma membrane protein transport Source: GO_Central
  3. Golgi vesicle docking Source: GO_Central
  4. intra-Golgi vesicle-mediated transport Source: GO_Central
  5. metabolic process Source: GOC
  6. plasma membrane fusion Source: UniProtKB
  7. positive regulation of receptor recycling Source: UniProtKB
  8. potassium ion transport Source: Ensembl
  9. regulation of exocytosis Source: ParkinsonsUK-UCL
  10. synaptic transmission Source: Reactome
  11. vesicle-mediated transport Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_18422. Trafficking of GluR2-containing AMPA receptors.

Protein family/group databases

TCDBi1.F.1.1.1. the synaptosomal vesicle fusion pore (svf-pore) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-fusing ATPase (EC:3.6.4.6)
Alternative name(s):
N-ethylmaleimide-sensitive fusion protein
Short name:
NEM-sensitive fusion protein
Vesicular-fusion protein NSF
Gene namesi
Name:NSF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componentsi: Chromosome 17, Unplaced

Organism-specific databases

HGNCiHGNC:8016. NSF.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: HPA
  2. cytosol Source: Reactome
  3. dendritic shaft Source: ParkinsonsUK-UCL
  4. extracellular vesicular exosome Source: UniProtKB
  5. Golgi stack Source: ParkinsonsUK-UCL
  6. lysosomal membrane Source: UniProtKB
  7. plasma membrane Source: UniProtKB
  8. postsynaptic density Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA31793.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 744744Vesicle-fusing ATPasePRO_0000084563Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei105 – 1051N6-acetyllysineBy similarity
Modified residuei259 – 2591PhosphotyrosineBy similarity
Modified residuei569 – 5691Phosphoserine; by CDK16By similarity

Post-translational modificationi

Phosphorylation at Ser-569 interferes with homohexamerization.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP46459.
PaxDbiP46459.
PRIDEiP46459.

2D gel databases

UCD-2DPAGEP46459.

PTM databases

PhosphoSiteiP46459.

Miscellaneous databases

PMAP-CutDBP46459.

Expressioni

Gene expression databases

BgeeiP46459.
CleanExiHS_NSF.
ExpressionAtlasiP46459. baseline and differential.
GenevestigatoriP46459.

Organism-specific databases

HPAiCAB009324.
CAB013645.
HPA003154.

Interactioni

Subunit structurei

Homohexamer. Interacts with GABARAP and GABARAPL2. Interacts with GRIA2. Interacts with PLK2, leading to disrupt the interaction with GRIA2. Interacts with MUSK; may regulate MUSK endocytosis and activity (By similarity). Interacts with CDK16 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
GABBR1Q9UBS53EBI-712251,EBI-724156
GABBR2O758994EBI-712251,EBI-715469
GRIA2P422622EBI-712251,EBI-3909876
NAPAP549202EBI-712251,EBI-749652
PTPN9P433782EBI-712251,EBI-742898

Protein-protein interaction databases

BioGridi110960. 41 interactions.
DIPiDIP-389N.
IntActiP46459. 38 interactions.
MINTiMINT-1369243.
STRINGi9606.ENSP00000381293.

Structurei

3D structure databases

ProteinModelPortaliP46459.
SMRiP46459. Positions 1-201, 220-742.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0464.
GeneTreeiENSGT00530000064085.
HOGENOMiHOG000198544.
HOVERGENiHBG000324.
InParanoidiP46459.
KOiK06027.
OMAiFKDKERS.
OrthoDBiEOG7P8P7F.
PhylomeDBiP46459.
TreeFamiTF300371.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
SM01073. CDC48_N. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P46459-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGRSMQAAR CPTDELSLTN CAVVNEKDFQ SGQHVIVRTS PNHRYTFTLK
60 70 80 90 100
THPSVVPGSI AFSLPQRKWA GLSIGQEIEV SLYTFDKAKQ CIGTMTIEID
110 120 130 140 150
FLQKKSIDSN PYDTDKMAAE FIQQFNNQAF SVGQQLVFSF NEKLFGLLVK
160 170 180 190 200
DIEAMDPSIL KGEPATGKRQ KIEVGLVVGN SQVAFEKAEN SSLNLIGKAK
210 220 230 240 250
TKENRQSIIN PDWNFEKMGI GGLDKEFSDI FRRAFASRVF PPEIVEQMGC
260 270 280 290 300
KHVKGILLYG PPGCGKTLLA RQIGKMLNAR EPKVVNGPEI LNKYVGESEA
310 320 330 340 350
NIRKLFADAE EEQRRLGANS GLHIIIFDEI DAICKQRGSM AGSTGVHDTV
360 370 380 390 400
VNQLLSKIDG VEQLNNILVI GMTNRPDLID EALLRPGRLE VKMEIGLPDE
410 420 430 440 450
KGRLQILHIH TARMRGHQLL SADVDIKELA VETKNFSGAE LEGLVRAAQS
460 470 480 490 500
TAMNRHIKAS TKVEVDMEKA ESLQVTRGDF LASLENDIKP AFGTNQEDYA
510 520 530 540 550
SYIMNGIIKW GDPVTRVLDD GELLVQQTKN SDRTPLVSVL LEGPPHSGKT
560 570 580 590 600
ALAAKIAEES NFPFIKICSP DKMIGFSETA KCQAMKKIFD DAYKSQLSCV
610 620 630 640 650
VVDDIERLLD YVPIGPRFSN LVLQALLVLL KKAPPQGRKL LIIGTTSRKD
660 670 680 690 700
VLQEMEMLNA FSTTIHVPNI ATGEQLLEAL ELLGNFKDKE RTTIAQQVKG
710 720 730 740
KKVWIGIKKL LMLIEMSLQM DPEYRVRKFL ALLREEGASP LDFD
Length:744
Mass (Da):82,594
Last modified:August 31, 2009 - v3
Checksum:iBF545A2A0C7EC2F7
GO
Isoform 2 (identifier: P46459-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-94: Missing.
     739-744: Missing.

Note: No experimental confirmation available.

Show »
Length:644
Mass (Da):71,584
Checksum:iACC3C42259713D42
GO

Sequence cautioni

The sequence AAA17411.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti22 – 221A → S in AAA17411 (Ref. 1) Curated
Sequence conflicti22 – 221A → S in AAF70545 (Ref. 2) Curated
Sequence conflicti25 – 251N → S in BAF82893 (PubMed:14702039).Curated
Sequence conflicti107 – 1071I → N in AAA17411 (Ref. 1) Curated
Sequence conflicti107 – 1071I → N in AAF70545 (Ref. 2) Curated
Sequence conflicti107 – 1071I → N in AAF04745 (Ref. 3) Curated
Sequence conflicti130 – 1301F → Y in AAA17411 (Ref. 1) Curated
Sequence conflicti130 – 1301F → Y in AAF70545 (Ref. 2) Curated
Sequence conflicti130 – 1301F → Y in AAF04745 (Ref. 3) Curated
Sequence conflicti154 – 1541A → S in AAA17411 (Ref. 1) Curated
Sequence conflicti154 – 1541A → S in AAF70545 (Ref. 2) Curated
Sequence conflicti154 – 1541A → S in AAF04745 (Ref. 3) Curated
Sequence conflicti237 – 2371S → F in AAA17411 (Ref. 1) Curated
Sequence conflicti237 – 2371S → F in AAF70545 (Ref. 2) Curated
Sequence conflicti237 – 2371S → F in AAF04745 (Ref. 3) Curated
Sequence conflicti251 – 2511K → I in AAA17411 (Ref. 1) Curated
Sequence conflicti251 – 2511K → I in AAF70545 (Ref. 2) Curated
Sequence conflicti251 – 2511K → I in AAF04745 (Ref. 3) Curated
Sequence conflicti427 – 4271K → R in BAF82893 (PubMed:14702039).Curated
Sequence conflicti571 – 5711D → E in AAF04745 (Ref. 3) Curated
Sequence conflicti639 – 6391K → M in BAF82893 (PubMed:14702039).Curated
Sequence conflicti686 – 6861F → L in AAA17411 (Ref. 1) Curated
Sequence conflicti686 – 6861F → L in AAF70545 (Ref. 2) Curated
Sequence conflicti686 – 6861F → L in AAF04745 (Ref. 3) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti476 – 4761T → M.
Corresponds to variant rs155733 [ dbSNP | Ensembl ].
VAR_029580

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 9494Missing in isoform 2. 1 PublicationVSP_056420Add
BLAST
Alternative sequencei739 – 7446Missing in isoform 2. 1 PublicationVSP_056421

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03985 mRNA. Translation: AAA17411.1. Different initiation.
AF135168 mRNA. Translation: AAF70545.1.
AF102846 mRNA. Translation: AAF04745.2.
AK290204 mRNA. Translation: BAF82893.1.
AK294001 mRNA. Translation: BAG57363.1.
AC004098 Genomic DNA. No translation available.
AC138645 Genomic DNA. No translation available.
AC138688 Genomic DNA. No translation available.
AC217769 Genomic DNA. No translation available.
AC217778 Genomic DNA. No translation available.
AC217780 Genomic DNA. No translation available.
BC030613 mRNA. Translation: AAH30613.1.
CCDSiCCDS42354.1. [P46459-1]
PIRiG01234.
RefSeqiNP_006169.2. NM_006178.3. [P46459-1]
UniGeneiHs.431279.

Genome annotation databases

EnsembliENST00000398238; ENSP00000381293; ENSG00000073969. [P46459-1]
ENST00000611398; ENSP00000478614; ENSG00000278174. [P46459-1]
ENST00000620290; ENSP00000481714; ENSG00000276262. [P46459-1]
GeneIDi4905.
KEGGihsa:4905.
UCSCiuc002iku.3. human. [P46459-1]

Polymorphism databases

DMDMi257051048.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03985 mRNA. Translation: AAA17411.1. Different initiation.
AF135168 mRNA. Translation: AAF70545.1.
AF102846 mRNA. Translation: AAF04745.2.
AK290204 mRNA. Translation: BAF82893.1.
AK294001 mRNA. Translation: BAG57363.1.
AC004098 Genomic DNA. No translation available.
AC138645 Genomic DNA. No translation available.
AC138688 Genomic DNA. No translation available.
AC217769 Genomic DNA. No translation available.
AC217778 Genomic DNA. No translation available.
AC217780 Genomic DNA. No translation available.
BC030613 mRNA. Translation: AAH30613.1.
CCDSiCCDS42354.1. [P46459-1]
PIRiG01234.
RefSeqiNP_006169.2. NM_006178.3. [P46459-1]
UniGeneiHs.431279.

3D structure databases

ProteinModelPortaliP46459.
SMRiP46459. Positions 1-201, 220-742.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110960. 41 interactions.
DIPiDIP-389N.
IntActiP46459. 38 interactions.
MINTiMINT-1369243.
STRINGi9606.ENSP00000381293.

Chemistry

ChEMBLiCHEMBL2311231.

Protein family/group databases

TCDBi1.F.1.1.1. the synaptosomal vesicle fusion pore (svf-pore) family.

PTM databases

PhosphoSiteiP46459.

Polymorphism databases

DMDMi257051048.

2D gel databases

UCD-2DPAGEP46459.

Proteomic databases

MaxQBiP46459.
PaxDbiP46459.
PRIDEiP46459.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000398238; ENSP00000381293; ENSG00000073969. [P46459-1]
ENST00000611398; ENSP00000478614; ENSG00000278174. [P46459-1]
ENST00000620290; ENSP00000481714; ENSG00000276262. [P46459-1]
GeneIDi4905.
KEGGihsa:4905.
UCSCiuc002iku.3. human. [P46459-1]

Organism-specific databases

CTDi4905.
GeneCardsiGC17P044668.
H-InvDBHIX0013914.
HIX0013915.
HGNCiHGNC:8016. NSF.
HPAiCAB009324.
CAB013645.
HPA003154.
MIMi601633. gene.
neXtProtiNX_P46459.
PharmGKBiPA31793.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0464.
GeneTreeiENSGT00530000064085.
HOGENOMiHOG000198544.
HOVERGENiHBG000324.
InParanoidiP46459.
KOiK06027.
OMAiFKDKERS.
OrthoDBiEOG7P8P7F.
PhylomeDBiP46459.
TreeFamiTF300371.

Enzyme and pathway databases

ReactomeiREACT_18422. Trafficking of GluR2-containing AMPA receptors.

Miscellaneous databases

ChiTaRSiNSF. human.
GeneWikiiN-ethylmaleimide_sensitive_fusion_protein.
GenomeRNAii4905.
NextBioi18875.
PMAP-CutDBP46459.
PROiP46459.
SOURCEiSearch...

Gene expression databases

BgeeiP46459.
CleanExiHS_NSF.
ExpressionAtlasiP46459. baseline and differential.
GenevestigatoriP46459.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
SM01073. CDC48_N. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and localization of a brain N-ethylmaleimide-sensitive factor involved in vesicular transport."
    Hong R., Moriyama Y., Mori H., Futai M., Yamamoto A., Tashiro Y., Fukui T., Tagaya M.
    Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. Bui T.D., Lu L., Hong W.
    Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "The regulation of hNSF gene expression."
    Zhang R., Liu Z., Wu M.
    Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Brain.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Cerebellum and Thalamus.
  5. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiNSF_HUMAN
AccessioniPrimary (citable) accession number: P46459
Secondary accession number(s): A8K2D9
, B4DFA2, Q8N6D7, Q9UKZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1995
Last sequence update: August 31, 2009
Last modified: March 31, 2015
This is version 151 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.