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Protein

D-glycero-beta-D-manno-heptose-1,7-bisphosphate 7-phosphatase

Gene

gmhB

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts the D-glycero-beta-D-manno-heptose 1,7-bisphosphate intermediate into D-glycero-beta-D-manno-heptose 1-phosphate by removing the phosphate group at the C-7 position in vitro. Also catalyzes the dephosphorylation of D-glycero-alpha-D-manno-heptose 1,7-bisphosphate, phospho-serine and fructose-1,6-biphosphate in vitro.1 Publication

Catalytic activityi

D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O = D-glycero-beta-D-manno-heptose 1-phosphate + phosphate.1 Publication

Cofactori

Protein has several cofactor binding sites:

Pathwayi: ADP-L-glycero-beta-D-manno-heptose biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Bifunctional protein HldE (hldE)
  2. D-glycero-beta-D-manno-heptose-1,7-bisphosphate 7-phosphatase (gmhB)
  3. Bifunctional protein HldE (hldE)
  4. ADP-L-glycero-D-manno-heptose-6-epimerase (hldD)
This subpathway is part of the pathway ADP-L-glycero-beta-D-manno-heptose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate, the pathway ADP-L-glycero-beta-D-manno-heptose biosynthesis and in Nucleotide-sugar biosynthesis.

Pathwayi: LOS core biosynthesis

This protein is involved in the pathway LOS core biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LOS core biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei8NucleophileBy similarity1
Metal bindingi8MagnesiumBy similarity1
Active sitei10Proton donorBy similarity1
Metal bindingi10Magnesium; via carbonyl oxygenBy similarity1
Sitei50Stabilizes the phosphoryl groupBy similarity1
Metal bindingi89ZincBy similarity1
Metal bindingi91Zinc; via pros nitrogenBy similarity1
Metal bindingi103ZincBy similarity1
Metal bindingi105ZincBy similarity1
Sitei106Contributes to substrate recognitionBy similarity1
Sitei107Stabilizes the phosphoryl groupBy similarity1
Metal bindingi132MagnesiumBy similarity1
Binding sitei133SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processCarbohydrate metabolism
LigandMagnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciHINF71421:G1GJ1-644-MONOMER
BRENDAi3.1.3.82 2529
UniPathwayiUPA00356; UER00438
UPA00976

Names & Taxonomyi

Protein namesi
Recommended name:
D-glycero-beta-D-manno-heptose-1,7-bisphosphate 7-phosphatase (EC:3.1.3.821 Publication)
Alternative name(s):
D,D-heptose 1,7-bisphosphate phosphatase
Short name:
HBP phosphatase
Gene namesi
Name:gmhB
Ordered Locus Names:HI_0621.1
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002093921 – 184D-glycero-beta-D-manno-heptose-1,7-bisphosphate 7-phosphataseAdd BLAST184

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi71421.HI0621.1

Structurei

3D structure databases

ProteinModelPortaliP46452
SMRiP46452
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni8 – 10Substrate bindingBy similarity3
Regioni16 – 19Substrate bindingBy similarity4
Regioni50 – 53Substrate bindingBy similarity4
Regioni106 – 107Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the GmhB family.Curated

Phylogenomic databases

eggNOGiENOG4108ZI0 Bacteria
COG0241 LUCA
KOiK03273
OMAiEHQICLE
PhylomeDBiP46452

Family and domain databases

Gene3Di3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR036412 HAD-like_sf
IPR006549 HAD-SF_hydro_IIIA
IPR023214 HAD_sf
IPR004446 Heptose_bisP_phosphatase
IPR006543 Histidinol-phos
PANTHERiPTHR42891 PTHR42891, 1 hit
PIRSFiPIRSF004682 GmhB, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR00213 GmhB_yaeD, 1 hit
TIGR01662 HAD-SF-IIIA, 1 hit
TIGR01656 Histidinol-ppas, 1 hit

Sequencei

Sequence statusi: Complete.

P46452-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKAIFLDRD GTLNIDYGYV HEIDNFKFID GVIDALRELK KMGYMLVLVT
60 70 80 90 100
NQSGIARGYF SEDQFLQLTE WMDWSLAEQD VDLDGIYYCP HHSEGKGEYK
110 120 130 140 150
EDCDCRKPKS GMLLQAIKEL KIDPTQSIMV GDKVEDLKAG IGAKVKMNVL
160 170 180
VRTGKPVTGE GEGIADYVLD SIVDLPRILK RLKK
Length:184
Mass (Da):20,810
Last modified:November 1, 1995 - v1
Checksum:i49D1D56DA6DF3DBD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA Translation: AAC22281.1
RefSeqiNP_438781.1, NC_000907.1
WP_005694551.1, NC_000907.1

Genome annotation databases

EnsemblBacteriaiAAC22281; AAC22281; HI_0621.1
GeneIDi949688
KEGGihin:HI0621.1
PATRICifig|71421.8.peg.647

Similar proteinsi

Entry informationi

Entry nameiGMHBB_HAEIN
AccessioniPrimary (citable) accession number: P46452
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 25, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health