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Protein

D-glycero-beta-D-manno-heptose-1,7-bisphosphate 7-phosphatase

Gene

gmhB

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Converts the D-glycero-beta-D-manno-heptose 1,7-bisphosphate intermediate into D-glycero-beta-D-manno-heptose 1-phosphate by removing the phosphate group at the C-7 position in vitro. Also catalyzes the dephosphorylation of D-glycero-alpha-D-manno-heptose 1,7-bisphosphate, phospho-serine and fructose-1,6-biphosphate in vitro.1 Publication

Catalytic activityi

D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O = D-glycero-beta-D-manno-heptose 1-phosphate + phosphate.1 Publication

Cofactori

Protein has several cofactor binding sites:

Pathwayi: ADP-L-glycero-beta-D-manno-heptose biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Bifunctional protein HldE (hldE)
  2. D-glycero-beta-D-manno-heptose-1,7-bisphosphate 7-phosphatase (gmhB)
  3. Bifunctional protein HldE (hldE)
  4. ADP-L-glycero-D-manno-heptose-6-epimerase (hldD)
This subpathway is part of the pathway ADP-L-glycero-beta-D-manno-heptose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate, the pathway ADP-L-glycero-beta-D-manno-heptose biosynthesis and in Nucleotide-sugar biosynthesis.

Pathwayi: LOS core biosynthesis

This protein is involved in the pathway LOS core biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LOS core biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei8NucleophileBy similarity1
Metal bindingi8MagnesiumBy similarity1
Active sitei10Proton donorBy similarity1
Metal bindingi10Magnesium; via carbonyl oxygenBy similarity1
Sitei50Stabilizes the phosphoryl groupBy similarity1
Metal bindingi89ZincBy similarity1
Metal bindingi91Zinc; via pros nitrogenBy similarity1
Metal bindingi103ZincBy similarity1
Metal bindingi105ZincBy similarity1
Sitei106Contributes to substrate recognitionBy similarity1
Sitei107Stabilizes the phosphoryl groupBy similarity1
Metal bindingi132MagnesiumBy similarity1
Binding sitei133SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.1.3.82. 2529.
UniPathwayiUPA00356; UER00438.
UPA00976.

Names & Taxonomyi

Protein namesi
Recommended name:
D-glycero-beta-D-manno-heptose-1,7-bisphosphate 7-phosphatase (EC:3.1.3.821 Publication)
Alternative name(s):
D,D-heptose 1,7-bisphosphate phosphatase
Short name:
HBP phosphatase
Gene namesi
Name:gmhB
Ordered Locus Names:HI_0621.1
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002093921 – 184D-glycero-beta-D-manno-heptose-1,7-bisphosphate 7-phosphataseAdd BLAST184

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi71421.HI0621.1.

Structurei

3D structure databases

ProteinModelPortaliP46452.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni8 – 10Substrate bindingBy similarity3
Regioni16 – 19Substrate bindingBy similarity4
Regioni50 – 53Substrate bindingBy similarity4
Regioni106 – 107Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the GmhB family.Curated

Phylogenomic databases

eggNOGiENOG4108ZI0. Bacteria.
COG0241. LUCA.
KOiK03273.
OMAiSFMIGDK.
PhylomeDBiP46452.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR006549. HAD-SF_hydro_IIIA.
IPR004446. Heptose_bisP_phosphatase.
IPR006543. Histidinol-phos.
[Graphical view]
PIRSFiPIRSF004682. GmhB. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR00213. GmhB_yaeD. 1 hit.
TIGR01662. HAD-SF-IIIA. 1 hit.
TIGR01656. Histidinol-ppas. 1 hit.

Sequencei

Sequence statusi: Complete.

P46452-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKAIFLDRD GTLNIDYGYV HEIDNFKFID GVIDALRELK KMGYMLVLVT
60 70 80 90 100
NQSGIARGYF SEDQFLQLTE WMDWSLAEQD VDLDGIYYCP HHSEGKGEYK
110 120 130 140 150
EDCDCRKPKS GMLLQAIKEL KIDPTQSIMV GDKVEDLKAG IGAKVKMNVL
160 170 180
VRTGKPVTGE GEGIADYVLD SIVDLPRILK RLKK
Length:184
Mass (Da):20,810
Last modified:November 1, 1995 - v1
Checksum:i49D1D56DA6DF3DBD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22281.1.
RefSeqiNP_438781.1. NC_000907.1.
WP_005694551.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22281; AAC22281; HI_0621.1.
GeneIDi949688.
KEGGihin:HI0621.1.
PATRICi20189829. VBIHaeInf48452_0647.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22281.1.
RefSeqiNP_438781.1. NC_000907.1.
WP_005694551.1. NC_000907.1.

3D structure databases

ProteinModelPortaliP46452.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0621.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC22281; AAC22281; HI_0621.1.
GeneIDi949688.
KEGGihin:HI0621.1.
PATRICi20189829. VBIHaeInf48452_0647.

Phylogenomic databases

eggNOGiENOG4108ZI0. Bacteria.
COG0241. LUCA.
KOiK03273.
OMAiSFMIGDK.
PhylomeDBiP46452.

Enzyme and pathway databases

UniPathwayiUPA00356; UER00438.
UPA00976.
BRENDAi3.1.3.82. 2529.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR006549. HAD-SF_hydro_IIIA.
IPR004446. Heptose_bisP_phosphatase.
IPR006543. Histidinol-phos.
[Graphical view]
PIRSFiPIRSF004682. GmhB. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR00213. GmhB_yaeD. 1 hit.
TIGR01662. HAD-SF-IIIA. 1 hit.
TIGR01656. Histidinol-ppas. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGMHBB_HAEIN
AccessioniPrimary (citable) accession number: P46452
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 5, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.