D,D-heptose 1,7-bisphosphate phosphatase - Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

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P46452 (GMHB_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 87. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
D,D-heptose 1,7-bisphosphate phosphatase
EC=3.1.3.-

Alternative name(s):
D-glycero-D-manno-heptose 1,7-bisphosphate phosphatase
HBP phosphatase

Gene names

Name:	gmhB
Ordered Locus Names:	HI_0621.1

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus ›

Protein attributes

Sequence length	184 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts the D-glycero-beta-D-manno-heptose 1,7-bisphosphate intermediate into D-glycero-beta-D-manno-heptose 1-phosphate by removing the phosphate group at the C-7 position By similarity.
Catalytic activity	D-glycero-D-manno-heptose 1,7-bisphosphate + H₂O = D-glycero-beta-D-manno-heptose 1-phosphate + phosphate.
Cofactor	Magnesium By similarity. Zinc By similarity.
Pathway	Nucleotide-sugar biosynthesis; ADP-L-glycero-beta-D-manno-heptose biosynthesis; ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate: step 2/4. Bacterial outer membrane biogenesis; LOS core biosynthesis.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	This enzyme is insensitive to the anomeric configuration of the D-glycero-D-manno-heptose 1,7-bisphosphate By similarity.
Sequence similarities	Belongs to the GmhB family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ADP-L-glycero-beta-D-manno-heptose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	D,D-heptose 1,7-bisphosphate phosphatase activity Inferred from sequence or structural similarity. Source: UniProtKB magnesium ion binding Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 184

184

D,D-heptose 1,7-bisphosphate phosphatase

PRO_0000209392

Regions

Region

8 – 10

Substrate binding By similarity

Region

16 – 19

Substrate binding By similarity

Region

50 – 53

Substrate binding By similarity

Region

106 – 107

Substrate binding By similarity

Sites

Active site

Nucleophile By similarity

Active site

Proton donor By similarity

Metal binding

Magnesium By similarity

Metal binding

Magnesium; via carbonyl oxygen By similarity

Metal binding

Zinc By similarity

Metal binding

Zinc; via amide nitrogen By similarity

Metal binding

103

Zinc By similarity

Metal binding

105

Zinc By similarity

Metal binding

132

Magnesium By similarity

Metal binding

133

Magnesium By similarity

Binding site

133

Substrate By similarity

Site

Stabilize the phosphoryl group By similarity

Site

106

Contributes to substrate recognition By similarity

Site

107

Stabilize the phosphoryl group By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P46452 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 49D1D56DA6DF3DBD
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FASTA

184

20,810

        10         20         30         40         50         60 
MNKAIFLDRD GTLNIDYGYV HEIDNFKFID GVIDALRELK KMGYMLVLVT NQSGIARGYF 

        70         80         90        100        110        120 
SEDQFLQLTE WMDWSLAEQD VDLDGIYYCP HHSEGKGEYK EDCDCRKPKS GMLLQAIKEL 

       130        140        150        160        170        180 
KIDPTQSIMV GDKVEDLKAG IGAKVKMNVL VRTGKPVTGE GEGIADYVLD SIVDLPRILK 


RLKK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. Venter J.C. Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]	Koonin E.V., Rudd K.E. Submitted (SEP-1995) to UniProtKB Cited for: IDENTIFICATION.
[3]	"Novel pathways for biosynthesis of nucleotide-activated glycero-manno-heptose precursors of bacterial glycoproteins and cell surface polysaccharides." Valvano M.A., Messner P., Kosma P. Microbiology 148:1979-1989(2002) [PubMed] [Europe PMC] [Abstract] Cited for: BIOSYNTHESIS OF NUCLEOTIDE-ACTIVATED GLYCERO-MANNO-HEPTOSE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC22281.1.
RefSeq	NP_438781.1. NC_000907.1.
3D structure databases
ProteinModelPortal	P46452.
SMR	P46452. Positions 4-181.
ModBase	Search...
Protein-protein interaction databases
STRING	71421.HI0621.1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC22281; AAC22281; HI_0621.1.
GeneID	949688.
KEGG	hin:HI0621.1.
PATRIC	20189829. VBIHaeInf48452_0647.
Phylogenomic databases
eggNOG	COG0241.
KO	K03273.
OMA	INIDKGY.
ProtClustDB	PRK08942.
Enzyme and pathway databases
UniPathway	UPA00356; UER00438. UPA00976.
Family and domain databases
Gene3D	3.40.50.1000. 1 hit.
InterPro	IPR023214. HAD-like_dom. IPR006549. HAD-SF_hydro_IIIA. IPR004446. Heptose_bisP_phosphatase. IPR006543. Histidinol-phos. [Graphical view]
PIRSF	PIRSF004682. GmhB. 1 hit.
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR00213. GmhB_yaeD. 1 hit. TIGR01662. HAD-SF-IIIA. 1 hit. TIGR01656. Histidinol-ppas. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GMHB_HAEIN

Accession

Primary (citable) accession number: P46452

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents