P46450 (LCFA_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Long-chain-fatty-acid--CoA ligase
EC=6.2.1.3

Alternative name(s):
Long-chain acyl-CoA synthetase

Gene names

Name:	fadD
Ordered Locus Names:	HI_0390.1

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › Haemophilus influenzae

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids By similarity.
Catalytic activity	ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.
Subunit structure	Homodimer Probable.
Subcellular location	Membrane; Peripheral membrane protein Potential. Note: Partially membrane-associated Potential.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	long-chain fatty acid metabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW long-chain fatty acid-CoA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 562	562	Long-chain-fatty-acid--CoA ligase		PRO_0000193131

Sequences

	Sequence		Length	Mass (Da)
	P46450 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 74BE7C8E9D711F12	FASTA	562	63,479
	10 20 30 40 50 60 MEKIWFQNYP KGSEKFLDTS KYESILDMFD KAVREHPDRP AYINMGQVLT FRKLEERSRA 70 80 90 100 110 120 FAAYLQNEFK LQRGDRVALM MPNLLQYPIA LFGILRAGLI AVNVNPLYTP RELELQLQDS 130 140 150 160 170 180 GAVAIVVVSN FASTLEKVVF NTNVKHVILT RMGDQLSFGK RTLVNFVVKY VKKLVPKYKL 190 200 210 220 230 240 PHAVTFREVL SIGKYRQYVR PEISREDLAF LQYTGGTTGV AKGAMLTHGN IITNVFQAKW 250 260 270 280 290 300 IAEPFIGDHS RTRSAILALP LYHVFALTVN CLLFLELGVT AILITNPRDI EGFVKELKKY 310 320 330 340 350 360 RFEAITGVNT LFNALLNNEN FKEVDFSALK LSVGGGMAIQ QSVATRWHEL TGCNIIEGYG 370 380 390 400 410 420 MTECSPLIAA CPINVVKHNG TIGVPVPNTD IKIIKDDGSD AKIGEAGELW VKGDQVMRGY 430 440 450 460 470 480 WQRPEATSEV LKDGWMATGD IVIMDESYSL RIVDRKKDII LVSGFNVYPN EIEDVVMLNY 490 500 510 520 530 540 KVSEAVAIGV PHAVSGETIK IFVVKKDDSL TRDELRNHCR QYLTGYKVPK EIEFRDELPK 550 560 TNVGKILRRV LRDEEIAKRP KH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]	Koonin E.V., Rudd K.E. Submitted (SEP-1995) to UniProtKB Cited for: IDENTIFICATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC22049.1.
RefSeq	NP_438551.1. NC_000907.1.
3D structure databases
ProteinModelPortal	P46450.
ModBase	Search...
Protein-protein interaction databases
STRING	71421.HI0390.1.
Proteomic databases
PRIDE	P46450.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC22049; AAC22049; HI_0390.1.
GeneID	950680.
KEGG	hin:HI0390.1.
PATRIC	20189329. VBIHaeInf48452_0408.
Phylogenomic databases
eggNOG	COG0318.
KO	K01897.
OMA	DQVMRGY.
ProtClustDB	PRK08974.
Family and domain databases
InterPro	IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. IPR025110. DUF4009. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. PF13193. DUF4009. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LCFA_HAEIN

Accession

Primary (citable) accession number: P46450

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program