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Protein

Formate dehydrogenase major subunit

Gene

fdxG

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Allows to use formate as major electron donor during anaerobic respiration. Subunit alpha possibly forms the active site.

Catalytic activityi

Formate + NAD+ = CO2 + NADH.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi50 – 501Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi53 – 531Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi57 – 571Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi100 – 1001Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. electron carrier activity Source: InterPro
  3. formate dehydrogenase (NAD+) activity Source: UniProtKB-EC
  4. molybdenum ion binding Source: InterPro

GO - Biological processi

  1. cellular respiration Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Formate dehydrogenase major subunit (EC:1.2.1.2)
Alternative name(s):
Formate dehydrogenase subunit alpha
Short name:
FDH subunit alpha
Gene namesi
Name:fdxG
Ordered Locus Names:HI_0006
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579: Chromosome

Subcellular locationi

Periplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: InterPro
  2. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Tat-type signalPROSITE-ProRule annotationAdd
BLAST
Chaini34 – 1028995Formate dehydrogenase major subunitPRO_0000063224Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Formate dehydrogenase is a membrane-bound complex, formed by subunits alpha, beta and gamma.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 114724Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

OMAiQNIRSMA.

Family and domain databases

InterProiIPR009010. Asp_de-COase-like_dom.
IPR006443. Formate_DH_asu_anaerob.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 2 hits.
TIGRFAMsiTIGR01553. formate-DH-alph. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46448-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQVSRRKFFK ICAGGMAGTS AAMLGFAPAN VLAAPREYKL LRAFESRNTC
60 70 80 90 100
TYCAVSCGML LYSTGKPYNS LSSHTGTNTR SKLFHIEGDP DHPVSRGALC
110 120 130 140 150
PKGAGSLDYV NSESRSLYPQ YRAPGSDKWE RISWKDAIKR IARLMKDDRD
160 170 180 190 200
ANFVEKDSNG KTVNRWATTG IMTASAMSNE AALLTQKWIR MLGMVPVCNQ
210 220 230 240 250
ANTUHGPTVA SLAPSFGRGA MTNNWVDIKN ANLIIVQGGN PAEAHPVGFR
260 270 280 290 300
WAIEAKKNGA KIIVIDPRFN RTASVADLHA PIRSGSDITF LMGVIRYLLE
310 320 330 340 350
TNQIQHEYVK HYTNASFLID EGFKFEDGLF VGYNEEKRNY DKSKWNYQFD
360 370 380 390 400
ENGHAKRDMT LQHPRCVINI LKEHVSRYTP EMVERITGVK QKLFLQICEE
410 420 430 440 450
IGKTSVPNKT MTHLYALGFT EHSIGTQNIR SMAIIQLLLG NMGMPGGGIN
460 470 480 490 500
ALRGHSNVQG TTDMGLLPMS LPGYMRLPND KDTSYDQYIN AITPKDIVPN
510 520 530 540 550
QVNYYRHTSK FFVSMMKTFY GDNATKENGW GFDFLPKADR LYDPITHVKL
560 570 580 590 600
MNEGKLHGWI LQGFNVLNSL PNKNKTLSGM SKLKYLVVMD PLQTESSEFW
610 620 630 640 650
RNFGESNNVN PAEIQTEVFR LPTTCFAEEE GSIVNSGRWT QWHWKGCDQP
660 670 680 690 700
GEALPDVDIL SMLREEMHEL YKKEGGQGIE SFEAMTWNYA QPHSPSAVEL
710 720 730 740 750
AKELNGYALE DLYDPNGNLM YKKGQLLNGF AHLRDDGTTT SGNWLYVGQW
760 770 780 790 800
TEKGNQTANR DNSDPSGLGC TIGWGFAWPA NRRVLYSRAS LDINGNPWDK
810 820 830 840 850
NRQLIKWNGK NWNWFDIADY GTQPPGSDTG PFIMSAEGVG RLFAVDKIAN
860 870 880 890 900
GPMPEHYEPV ESPIDTNPFH PNVVTDPTLR IYKEDREFIG SNKEYPFVAT
910 920 930 940 950
TYRLTEHFHS WTAQSALNII AQPQQFVEIG EKLAAEKGIQ KGDMVKITSR
960 970 980 990 1000
RGYIKAVAVV TKRLKDLEID GRVVHHIGLP IHWNMKALNG KGNRGFSTNT
1010 1020
LTPSWGEAIT QTPEYKTFLV NIEKVGEA
Length:1,028
Mass (Da):115,451
Last modified:February 26, 2008 - v2
Checksum:i4A2E55B78B301300
GO

Non-standard residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-standard residuei204 – 2041SelenocysteineCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. No translation available.

Keywords - Coding sequence diversityi

Selenocysteine

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. No translation available.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

OMAiQNIRSMA.

Family and domain databases

InterProiIPR009010. Asp_de-COase-like_dom.
IPR006443. Formate_DH_asu_anaerob.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 2 hits.
TIGRFAMsiTIGR01553. formate-DH-alph. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiFDXG_HAEIN
AccessioniPrimary (citable) accession number: P46448
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: February 26, 2008
Last modified: January 7, 2015
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.