P46434 (GST1_ONCVO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase 1
EC=2.5.1.18

Gene names

Name:	GST1
Synonyms:	GSTA

Organism

Onchocerca volvulus

Taxonomic identifier

6282 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Spirurida › Filarioidea › Onchocercidae › Onchocerca

Protein attributes

Sequence length	235 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer By similarity. Ref.2
Sequence similarities	Belongs to the GST superfamily. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Molecular function	Transferase
Technical term	3D-structure
Gene Ontology (GO)
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

‹1 – 235

›235

Glutathione S-transferase 1

PRO_0000185969

Regions

Domain

36 – 113

78

GST N-terminal

Domain

115 – 235

121

GST C-terminal

Region

97 – 98

2

Glutathione binding

Sites

Binding site

42

1

Glutathione

Binding site

73

1

Glutathione

Binding site

77

1

Glutathione

Binding site

85

1

Glutathione; via amide nitrogen and carbonyl oxygen

Experimental info

Non-terminal residue

1

1

Secondary structure

1

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235

Helix Strand Turn

Details...

Beta strand

38 – 46

9

Helix

47 – 49

3

Helix

50 – 59

10

Beta strand

64 – 68

5

Helix

70 – 76

7

Helix

77 – 79

3

Beta strand

80 – 83

4

Beta strand

87 – 90

4

Beta strand

93 – 96

4

Helix

98 – 108

11

Helix

116 – 141

26

Helix

145 – 156

12

Turn

157 – 159

3

Helix

160 – 172

13

Beta strand

175 – 177

3

Helix

186 – 197

12

Helix

199 – 201

3

Helix

204 – 206

3

Helix

208 – 219

12

Helix

223 – 229

7

Sequences

	Sequence		Length	Mass (Da)
	P46434 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: F28928E44102F63A	FASTA	235	26,838
	10 20 30 40 50 60 FITMNFVVEA ASSNANQAIT SENSIKPKGK LQPQMEKYTL TYFNGRGRAE VIRLLFALAN 70 80 90 100 110 120 VSYEDNRITR DEWKYLKPRT PFGHVPMLNV SGNVLGESHA IELLLGGRFG LLGTNDWEEA 130 140 150 160 170 180 KIMAVVLNID ELFQKLIPWT HEKNTTKKAE LFRNLSESDV MPFLGRYEKF LKESTTGHIV 190 200 210 220 230 GNKVSVADLT VFNMLMTLDD EVKLEEYPQL ASFVNKIGQM PGIKEWIKKR PKTYF

References

[1]	"Isolation, sequence and expression of an Onchocerca volvulus glutathione S-transferase cDNA." Liebau E., Walter R.D., Henkle-Duehrsen K. Mol. Biochem. Parasitol. 63:305-309(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structure of the extracellular glutathione S-transferase OvGST1 from the human pathogenic parasite Onchocerca volvulus." Perbandt M., Hoppner J., Burmeister C., Luersen K., Betzel C., Liebau E. J. Mol. Biol. 377:501-511(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 11-235 IN COMPLEX WITH GLUTATHIONE, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X75029 mRNA. Translation: CAA52937.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2HNL	X-ray	2.00	A/B	11-235	[»]

ProteinModelPortal

P46434.

SMR

P46434. Positions 34-235.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.

InterPro

IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR017933. Glutathione_S_Trfase/Cl_chnl_C.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]

Pfam

PF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]

SUPFAM

SSF47616. GST_C_like. 1 hit.
SSF52833. Thiordxn-like_fd. 1 hit.

PROSITE

PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P46434.

Entry information

Entry name

GST1_ONCVO

Accession

Primary (citable) accession number: P46434

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)