P46428 (GST_ANOGA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase
EC=2.5.1.18

Alternative name(s):
GST class-sigma

Gene names

Name:	GstS1
ORF Names:	AGAP010404

Organism

Anopheles gambiae (African malaria mosquito) [Reference proteome]

Taxonomic identifier

7165 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicimorpha › Culicoidea › Culicidae › Anophelinae › Anopheles › Cellia › Pyretophorus › gambiae species complex

Protein attributes

Sequence length	203 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the GST superfamily. Sigma family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.
Sequence caution	The sequence AAA29358.1 differs from that shown. Reason: Erroneous initiation. The sequence EAA45010.4 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: P46428-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform B (identifier: P46428-2)

The sequence of this isoform differs from the canonical sequence as follows:
     60-95: KVHQSVAMSRYLANQVGLAGADDWENLMIDTVVDTV → RVHQSLAMCRYVAKQINLAGDNPLEALQIDAIVDTI

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 203	203	Glutathione S-transferase		PRO_0000185915
Regions
	Domain	2 – 79	78	GST N-terminal
	Domain	81 – 203	123	GST C-terminal
	Region	50 – 51	2	Glutathione binding By similarity
	Region	63 – 64	2	Glutathione binding By similarity
Sites
	Binding site	8	1	Glutathione By similarity
	Binding site	39	1	Glutathione By similarity
	Binding site	43	1	Glutathione By similarity
Natural variations
	Alternative sequence	60 – 95	36	KVHQS…VVDTV → RVHQSLAMCRYVAKQINLAG DNPLEALQIDAIVDTI in isoform B.		VSP_029900
Experimental info
	Sequence conflict	49	1	G → R in AAA29358. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified December 4, 2007. Version 4. Checksum: 99EF5E78AA1A7595	FASTA	203	23,246
	10 20 30 40 50 60 MPDYKVYYFN VKALGEPLRF LLSYGNLPFD DVRITREEWP ALKPTMPMGQ MPVLEVDGKK 70 80 90 100 110 120 VHQSVAMSRY LANQVGLAGA DDWENLMIDT VVDTVNDFRL KIAIVAYEPD DMVKEKKMVT 130 140 150 160 170 180 LNNEVIPFYL TKLNVIAKEN NGHLVLGKPT WADVYFAGIL DYLNYLTKTN LLENFPNLQE 190 200 VVQKVLDNEN VKAYIAKRPI TEV
	Isoform B [UniParc]. Checksum: 403F5B0F0057BC28	FASTA	203	23,279
	10 20 30 40 50 60 MPDYKVYYFN VKALGEPLRF LLSYGNLPFD DVRITREEWP ALKPTMPMGQ MPVLEVDGKR 70 80 90 100 110 120 VHQSLAMCRY VAKQINLAGD NPLEALQIDA IVDTINDFRL KIAIVAYEPD DMVKEKKMVT 130 140 150 160 170 180 LNNEVIPFYL TKLNVIAKEN NGHLVLGKPT WADVYFAGIL DYLNYLTKTN LLENFPNLQE 190 200 VVQKVLDNEN VKAYIAKRPI TEV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A glutathione S-transferase gene of the vector mosquito, Anopheles gambiae." Reiss R.A., James A.A. Insect Mol. Biol. 2:25-32(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B). Strain: G3.
[2]	"The genome sequence of the malaria mosquito Anopheles gambiae." Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R., Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R., Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z., Kraft C.L., Abril J.F. , Anthouard V., Arensburger P., Atkinson P.W., Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C., Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K., Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V., Dana A., Delcher A., Dew I., Evans C.A., Flanigan M., Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R., Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J., Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I., Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A., McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D., O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H., Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J., Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B., Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M., Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I., Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J., Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M., Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C., Collins F.H., Hoffman S.L. Science 298:129-149(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PEST.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L07880 mRNA. Translation: AAA29358.1. Different initiation. AAAB01008849 Genomic DNA. Translation: EAA45010.4. Different initiation.
RefSeq	XP_311546.4. XM_311546.4.
3D structure databases
ProteinModelPortal	P46428.
SMR	P46428. Positions 4-203.
ModBase	Search...
Protein-protein interaction databases
STRING	7165.AGAP010404-PA.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1272645.
KEGG	aga:AgaP_AGAP010404.
VectorBase	AGAP010404. Anopheles gambiae.
Organism-specific databases
CTD	1272645.
Phylogenomic databases
eggNOG	NOG122057.
HOGENOM	HOG000115733.
InParanoid	P46428.
OrthoDB	EOG4N02WZ.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GST_ANOGA

Accession

Primary (citable) accession number: P46428
Secondary accession number(s): Q7PGA2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	December 4, 2007
Last modified:	April 3, 2013
This is version 78 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)