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Protein

Glutathione S-transferase F2

Gene

GSTF2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds auxin, endogenous flavonoids and the phytoalexin camalexin and may be involved in regulating the binding and transport of small bioactive natural products and defense-related compounds during plant stress. Binds a series of heterocyclic compounds, including lumichrome, harmane, norharmane and indole-3-aldehyde. In vitro, possesses glutathione S-transferase activity toward 1-chloro-2,4-dinitrobenzene (CDNB) and benzyl isothiocyanate (BITC). Acts as glutathione peroxidase on cumene hydroperoxide, linoleic acid-13-hydroperoxide and trans-stilbene oxide, but not trans-cinnamic acid or IAA-CoA.2 Publications

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

GO - Molecular functioni

  • camalexin binding Source: TAIR
  • glutathione binding Source: TAIR
  • glutathione transferase activity Source: TAIR
  • peroxidase activity Source: UniProtKB-KW
  • quercitrin binding Source: TAIR

GO - Biological processi

  • auxin-activated signaling pathway Source: UniProtKB-KW
  • defense response to bacterium Source: TAIR
  • defense response to fungus Source: TAIR
  • glutathione metabolic process Source: GO_Central
  • response to cadmium ion Source: TAIR
  • response to cold Source: TAIR
  • response to salt stress Source: TAIR
  • response to zinc ion Source: TAIR
  • toxin catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase, Transferase

Keywords - Biological processi

Auxin signaling pathway, Detoxification, Plant defense, Stress response

Enzyme and pathway databases

BioCyciARA:AT4G02520-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase F2 (EC:2.5.1.18)
Short name:
AtGSTF2
Alternative name(s):
24 kDa auxin-binding protein
Short name:
AtPM24
GST class-phi member 2
Gene namesi
Name:GSTF2
Synonyms:PM24.1
Ordered Locus Names:At4g02520
ORF Names:T10P11.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G02520.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
  • cytosol Source: TAIR
  • endoplasmic reticulum Source: UniProtKB-SubCell
  • intracellular membrane-bounded organelle Source: TAIR
  • membrane Source: TAIR
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001858481 – 212Glutathione S-transferase F2Add BLAST212

Proteomic databases

PaxDbiP46422.
PRIDEiP46422.

2D gel databases

SWISS-2DPAGEP46422.

PTM databases

iPTMnetiP46422.

Expressioni

Tissue specificityi

Expressed in the root-shoot transition zone and root tips.1 Publication

Inductioni

By ethylene, auxin, glutathione, salicylic acid, copper, paraquat, acetochlor, metolachlor and the pathogens P.syringae and Hyaloperonospora parasitica.6 Publications

Gene expression databases

GenevisibleiP46422. AT.

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

BioGridi13222. 7 interactors.
STRINGi3702.AT4G02520.1.

Structurei

Secondary structure

1212
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 8Combined sources5
Helixi13 – 24Combined sources12
Beta strandi30 – 33Combined sources4
Helixi36 – 38Combined sources3
Helixi40 – 42Combined sources3
Helixi46 – 49Combined sources4
Beta strandi57 – 60Combined sources4
Beta strandi63 – 67Combined sources5
Helixi68 – 78Combined sources11
Turni79 – 81Combined sources3
Beta strandi82 – 84Combined sources3
Helixi94 – 109Combined sources16
Helixi112 – 122Combined sources11
Helixi124 – 127Combined sources4
Helixi134 – 157Combined sources24
Beta strandi158 – 160Combined sources3
Beta strandi163 – 165Combined sources3
Helixi168 – 171Combined sources4
Helixi174 – 180Combined sources7
Helixi186 – 190Combined sources5
Helixi193 – 204Combined sources12
Helixi206 – 211Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BX9X-ray2.60A2-212[»]
1GNWX-ray2.20A/B2-212[»]
5A4UX-ray2.00A/B/C/D/E/F1-212[»]
5A4VX-ray2.38A/B/C/D/E/F1-212[»]
5A4WX-ray2.25A/B/C/D/E/F1-212[»]
5A5KX-ray2.77A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X1-212[»]
ProteinModelPortaliP46422.
SMRiP46422.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP46422.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 83GST N-terminalAdd BLAST82
Domaini93 – 212GST C-terminalAdd BLAST120

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni12 – 13Glutathione binding2
Regioni41 – 42Glutathione binding2
Regioni54 – 55Glutathione binding2
Regioni67 – 68Glutathione binding2

Sequence similaritiesi

Belongs to the GST superfamily. Phi family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiKOG0867. Eukaryota.
COG0625. LUCA.
HOGENOMiHOG000125746.
InParanoidiP46422.
KOiK00799.
OMAiQYAIMAI.
OrthoDBiEOG09360M18.
PhylomeDBiP46422.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46422-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGIKVFGHP ASIATRRVLI ALHEKNLDFE LVHVELKDGE HKKEPFLSRN
60 70 80 90 100
PFGQVPAFED GDLKLFESRA ITQYIAHRYE NQGTNLLQTD SKNISQYAIM
110 120 130 140 150
AIGMQVEDHQ FDPVASKLAF EQIFKSIYGL TTDEAVVAEE EAKLAKVLDV
160 170 180 190 200
YEARLKEFKY LAGETFTLTD LHHIPAIQYL LGTPTKKLFT ERPRVNEWVA
210
EITKRPASEK VQ
Length:212
Mass (Da):24,129
Last modified:January 23, 2007 - v3
Checksum:i90A1CBEEEBAC815B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75303 mRNA. Translation: CAA53051.1.
L07589 mRNA. Translation: AAA32800.1.
L11601 mRNA. Translation: AAA32801.1.
AC002330 Genomic DNA. Translation: AAC78264.1.
AL161494 Genomic DNA. Translation: CAB80745.1.
CP002687 Genomic DNA. Translation: AEE82183.1.
AF324681 mRNA. Translation: AAG40032.1.
AF326903 mRNA. Translation: AAG41485.1.
AF349527 mRNA. Translation: AAK15574.1.
AY039580 mRNA. Translation: AAK62635.1.
AY056082 mRNA. Translation: AAL06970.1.
PIRiS35268.
RefSeqiNP_192161.1. NM_116486.3.
UniGeneiAt.22195.
At.24972.

Genome annotation databases

EnsemblPlantsiAT4G02520.1; AT4G02520.1; AT4G02520.
GeneIDi827931.
GrameneiAT4G02520.1; AT4G02520.1; AT4G02520.
KEGGiath:AT4G02520.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75303 mRNA. Translation: CAA53051.1.
L07589 mRNA. Translation: AAA32800.1.
L11601 mRNA. Translation: AAA32801.1.
AC002330 Genomic DNA. Translation: AAC78264.1.
AL161494 Genomic DNA. Translation: CAB80745.1.
CP002687 Genomic DNA. Translation: AEE82183.1.
AF324681 mRNA. Translation: AAG40032.1.
AF326903 mRNA. Translation: AAG41485.1.
AF349527 mRNA. Translation: AAK15574.1.
AY039580 mRNA. Translation: AAK62635.1.
AY056082 mRNA. Translation: AAL06970.1.
PIRiS35268.
RefSeqiNP_192161.1. NM_116486.3.
UniGeneiAt.22195.
At.24972.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BX9X-ray2.60A2-212[»]
1GNWX-ray2.20A/B2-212[»]
5A4UX-ray2.00A/B/C/D/E/F1-212[»]
5A4VX-ray2.38A/B/C/D/E/F1-212[»]
5A4WX-ray2.25A/B/C/D/E/F1-212[»]
5A5KX-ray2.77A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X1-212[»]
ProteinModelPortaliP46422.
SMRiP46422.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi13222. 7 interactors.
STRINGi3702.AT4G02520.1.

PTM databases

iPTMnetiP46422.

2D gel databases

SWISS-2DPAGEP46422.

Proteomic databases

PaxDbiP46422.
PRIDEiP46422.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G02520.1; AT4G02520.1; AT4G02520.
GeneIDi827931.
GrameneiAT4G02520.1; AT4G02520.1; AT4G02520.
KEGGiath:AT4G02520.

Organism-specific databases

TAIRiAT4G02520.

Phylogenomic databases

eggNOGiKOG0867. Eukaryota.
COG0625. LUCA.
HOGENOMiHOG000125746.
InParanoidiP46422.
KOiK00799.
OMAiQYAIMAI.
OrthoDBiEOG09360M18.
PhylomeDBiP46422.

Enzyme and pathway databases

BioCyciARA:AT4G02520-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP46422.
PROiP46422.

Gene expression databases

GenevisibleiP46422. AT.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSTF2_ARATH
AccessioniPrimary (citable) accession number: P46422
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 142 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.