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Protein

Glutathione S-transferase F2

Gene

GSTF2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds auxin, endogenous flavonoids and the phytoalexin camalexin and may be involved in regulating the binding and transport of small bioactive natural products and defense-related compounds during plant stress. Binds a series of heterocyclic compounds, including lumichrome, harmane, norharmane and indole-3-aldehyde. In vitro, possesses glutathione S-transferase activity toward 1-chloro-2,4-dinitrobenzene (CDNB) and benzyl isothiocyanate (BITC). Acts as glutathione peroxidase on cumene hydroperoxide, linoleic acid-13-hydroperoxide and trans-stilbene oxide, but not trans-cinnamic acid or IAA-CoA.2 Publications

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

GO - Molecular functioni

  • camalexin binding Source: TAIR
  • glutathione binding Source: TAIR
  • glutathione transferase activity Source: TAIR
  • peroxidase activity Source: UniProtKB-KW
  • protein domain specific binding Source: CAFA
  • quercitrin binding Source: TAIR

GO - Biological processi

  • auxin-activated signaling pathway Source: UniProtKB-KW
  • defense response to bacterium Source: TAIR
  • glutathione metabolic process Source: GO_Central
  • response to cadmium ion Source: TAIR
  • response to cold Source: TAIR
  • response to oomycetes Source: TAIR
  • response to salt stress Source: TAIR
  • response to zinc ion Source: TAIR
  • toxin catabolic process Source: TAIR

Keywordsi

Molecular functionOxidoreductase, Peroxidase, Transferase
Biological processAuxin signaling pathway, Detoxification, Plant defense, Stress response

Enzyme and pathway databases

BioCyciARA:AT4G02520-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase F2 (EC:2.5.1.18)
Short name:
AtGSTF2
Alternative name(s):
24 kDa auxin-binding protein
Short name:
AtPM24
GST class-phi member 2
Gene namesi
Name:GSTF2
Synonyms:PM24.1
Ordered Locus Names:At4g02520
ORF Names:T10P11.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G02520
TAIRilocus:2132308 AT4G02520

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001858481 – 212Glutathione S-transferase F2Add BLAST212

Proteomic databases

PaxDbiP46422
PRIDEiP46422

2D gel databases

SWISS-2DPAGEiP46422

PTM databases

iPTMnetiP46422

Expressioni

Tissue specificityi

Expressed in the root-shoot transition zone and root tips.1 Publication

Inductioni

By ethylene, auxin, glutathione, salicylic acid, copper, paraquat, acetochlor, metolachlor and the pathogens P.syringae and Hyaloperonospora parasitica.6 Publications

Gene expression databases

ExpressionAtlasiP46422 baseline and differential
GenevisibleiP46422 AT

Interactioni

Subunit structurei

Homodimer.2 Publications

GO - Molecular functioni

  • protein domain specific binding Source: CAFA

Protein-protein interaction databases

BioGridi13222, 7 interactors
IntActiP46422, 1 interactor
STRINGi3702.AT4G02520.1

Structurei

Secondary structure

1212
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 8Combined sources5
Helixi13 – 24Combined sources12
Beta strandi30 – 33Combined sources4
Helixi36 – 38Combined sources3
Helixi40 – 42Combined sources3
Helixi46 – 49Combined sources4
Beta strandi57 – 60Combined sources4
Beta strandi63 – 67Combined sources5
Helixi68 – 78Combined sources11
Turni79 – 81Combined sources3
Beta strandi82 – 84Combined sources3
Helixi94 – 109Combined sources16
Helixi112 – 122Combined sources11
Helixi124 – 127Combined sources4
Helixi134 – 157Combined sources24
Beta strandi158 – 160Combined sources3
Beta strandi163 – 165Combined sources3
Helixi168 – 171Combined sources4
Helixi174 – 180Combined sources7
Helixi186 – 190Combined sources5
Helixi193 – 204Combined sources12
Helixi206 – 211Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BX9X-ray2.60A2-212[»]
1GNWX-ray2.20A/B2-212[»]
5A4UX-ray2.00A/B/C/D/E/F1-212[»]
5A4VX-ray2.38A/B/C/D/E/F1-212[»]
5A4WX-ray2.25A/B/C/D/E/F1-212[»]
5A5KX-ray2.77A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X1-212[»]
ProteinModelPortaliP46422
SMRiP46422
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP46422

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 83GST N-terminalAdd BLAST82
Domaini93 – 212GST C-terminalAdd BLAST120

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni12 – 13Glutathione binding2
Regioni41 – 42Glutathione binding2
Regioni54 – 55Glutathione binding2
Regioni67 – 68Glutathione binding2

Sequence similaritiesi

Belongs to the GST superfamily. Phi family.Curated

Phylogenomic databases

eggNOGiKOG0867 Eukaryota
COG0625 LUCA
HOGENOMiHOG000125746
InParanoidiP46422
KOiK00799
OMAiPHWLENP
OrthoDBiEOG09360M18
PhylomeDBiP46422

Family and domain databases

CDDicd03187 GST_C_Phi, 1 hit
InterProiView protein in InterPro
IPR010987 Glutathione-S-Trfase_C-like
IPR036282 Glutathione-S-Trfase_C_sf
IPR004045 Glutathione_S-Trfase_N
IPR004046 GST_C
IPR034347 GST_Phi_C
IPR036249 Thioredoxin-like_sf
PfamiView protein in Pfam
PF00043 GST_C, 1 hit
PF02798 GST_N, 1 hit
SUPFAMiSSF47616 SSF47616, 1 hit
SSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS50405 GST_CTER, 1 hit
PS50404 GST_NTER, 1 hit

Sequencei

Sequence statusi: Complete.

P46422-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGIKVFGHP ASIATRRVLI ALHEKNLDFE LVHVELKDGE HKKEPFLSRN
60 70 80 90 100
PFGQVPAFED GDLKLFESRA ITQYIAHRYE NQGTNLLQTD SKNISQYAIM
110 120 130 140 150
AIGMQVEDHQ FDPVASKLAF EQIFKSIYGL TTDEAVVAEE EAKLAKVLDV
160 170 180 190 200
YEARLKEFKY LAGETFTLTD LHHIPAIQYL LGTPTKKLFT ERPRVNEWVA
210
EITKRPASEK VQ
Length:212
Mass (Da):24,129
Last modified:January 23, 2007 - v3
Checksum:i90A1CBEEEBAC815B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75303 mRNA Translation: CAA53051.1
L07589 mRNA Translation: AAA32800.1
L11601 mRNA Translation: AAA32801.1
AC002330 Genomic DNA Translation: AAC78264.1
AL161494 Genomic DNA Translation: CAB80745.1
CP002687 Genomic DNA Translation: AEE82183.1
AF324681 mRNA Translation: AAG40032.1
AF326903 mRNA Translation: AAG41485.1
AF349527 mRNA Translation: AAK15574.1
AY039580 mRNA Translation: AAK62635.1
AY056082 mRNA Translation: AAL06970.1
PIRiS35268
RefSeqiNP_192161.1, NM_116486.3
UniGeneiAt.22195
At.24972

Genome annotation databases

EnsemblPlantsiAT4G02520.1; AT4G02520.1; AT4G02520
GeneIDi827931
GrameneiAT4G02520.1; AT4G02520.1; AT4G02520
KEGGiath:AT4G02520

Similar proteinsi

Entry informationi

Entry nameiGSTF2_ARATH
AccessioniPrimary (citable) accession number: P46422
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 152 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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