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P46421

- GSTU5_ARATH

UniProt

P46421 - GSTU5_ARATH

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Protein

Glutathione S-transferase U5

Gene

GSTU5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

In vitro, possesses glutathione S-transferase activity toward 1-chloro-2,4-dinitrobenzene (CDNB) and benzyl isothiocyanate (BITC). May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides.1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

GO - Molecular functioni

  1. glutathione binding Source: TAIR
  2. glutathione transferase activity Source: UniProtKB-EC

GO - Biological processi

  1. response to oxidative stress Source: TAIR
  2. response to toxic substance Source: UniProtKB-KW
  3. toxin catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Detoxification, Stress response

Enzyme and pathway databases

BioCyciARA:AT2G29450-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase U5 (EC:2.5.1.18)
Short name:
AtGSTU5
Alternative name(s):
AtGSTU1
GST class-tau member 5
Glutathione S-transferase 103-1A
Gene namesi
Name:GSTU5
Synonyms:GSTU1
Ordered Locus Names:At2g29450
ORF Names:F16P2.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G29450.

Subcellular locationi

Cytoplasmcytosol Curated

GO - Cellular componenti

  1. cytoplasm Source: TAIR
  2. cytosol Source: TAIR
  3. plasma membrane Source: TAIR
  4. plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 224224Glutathione S-transferase U5PRO_0000185860Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei151 – 1511PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP46421.
PRIDEiP46421.

Expressioni

Inductioni

By auxin.

Gene expression databases

ExpressionAtlasiP46421. baseline and differential.
GenevestigatoriP46421.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G29450.1-P.

Structurei

3D structure databases

ProteinModelPortaliP46421.
SMRiP46421. Positions 5-209.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 8480GST N-terminalAdd
BLAST
Domaini89 – 217129GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni15 – 162Glutathione bindingBy similarity
Regioni41 – 422Glutathione bindingBy similarity
Regioni55 – 562Glutathione bindingBy similarity
Regioni68 – 692Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the GST superfamily. Tau family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiCOG0625.
HOGENOMiHOG000125749.
InParanoidiP46421.
KOiK00799.
OMAiERSKARF.
PhylomeDBiP46421.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46421-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAEKEEVKLL GIWASPFSRR VEMALKLKGI PYEYVEEILE NKSPLLLALN
60 70 80 90 100
PIHKKVPVLV HNGKTILESH VILEYIDETW PQNPILPQDP YERSKARFFA
110 120 130 140 150
KLVDEQIMNV GFISMARADE KGREVLAEQV RELIMYLEKE LVGKDYFGGK
160 170 180 190 200
TVGFLDFVAG SLIPFCLERG WEGIGLEVIT EEKFPEFKRW VRNLEKVEIV
210 220
KDCVPPREEH VEHMNYMAER VRSS
Length:224
Mass (Da):26,000
Last modified:November 1, 1995 - v1
Checksum:iBA2F5C06B94FFCC4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X89216 Genomic DNA. Translation: CAA61504.1.
U30489 Genomic DNA. Translation: AAA74019.1.
D44465 mRNA. Translation: BAA07917.1.
AF144382 mRNA. Translation: AAD34992.1.
AC004561 Genomic DNA. Translation: AAC95193.1.
CP002685 Genomic DNA. Translation: AEC08255.1.
AY062676 mRNA. Translation: AAL32754.1.
BT001228 mRNA. Translation: AAN65115.1.
AY088413 mRNA. Translation: AAM65950.1.
PIRiS66354.
RefSeqiNP_180506.1. NM_128499.4.
UniGeneiAt.19941.

Genome annotation databases

EnsemblPlantsiAT2G29450.1; AT2G29450.1; AT2G29450.
GeneIDi817494.
KEGGiath:AT2G29450.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X89216 Genomic DNA. Translation: CAA61504.1 .
U30489 Genomic DNA. Translation: AAA74019.1 .
D44465 mRNA. Translation: BAA07917.1 .
AF144382 mRNA. Translation: AAD34992.1 .
AC004561 Genomic DNA. Translation: AAC95193.1 .
CP002685 Genomic DNA. Translation: AEC08255.1 .
AY062676 mRNA. Translation: AAL32754.1 .
BT001228 mRNA. Translation: AAN65115.1 .
AY088413 mRNA. Translation: AAM65950.1 .
PIRi S66354.
RefSeqi NP_180506.1. NM_128499.4.
UniGenei At.19941.

3D structure databases

ProteinModelPortali P46421.
SMRi P46421. Positions 5-209.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT2G29450.1-P.

Proteomic databases

PaxDbi P46421.
PRIDEi P46421.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G29450.1 ; AT2G29450.1 ; AT2G29450 .
GeneIDi 817494.
KEGGi ath:AT2G29450.

Organism-specific databases

TAIRi AT2G29450.

Phylogenomic databases

eggNOGi COG0625.
HOGENOMi HOG000125749.
InParanoidi P46421.
KOi K00799.
OMAi ERSKARF.
PhylomeDBi P46421.

Enzyme and pathway databases

BioCyci ARA:AT2G29450-MONOMER.

Gene expression databases

ExpressionAtlasi P46421. baseline and differential.
Genevestigatori P46421.

Family and domain databases

Gene3Di 1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProi IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR012336. Thioredoxin-like_fold.
[Graphical view ]
Pfami PF13417. GST_N_3. 1 hit.
[Graphical view ]
SUPFAMi SSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEi PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of an auxin-inducible glutathione S-transferase gene of Arabidopsis thaliana."
    van der Kop D.A.M., Schuyer M., Scheres B., van der Zaal B.J., Hooykaas P.J.J.
    Plant Mol. Biol. 30:839-844(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Landsberg erecta.
  2. "Auxins and their related substances competitively inhibit enzymatic activity of an Arabidopsis glutathione S-transferase isozyme expressed in Escherichia coli."
    Watahiki M., Yamamoto K.
    Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
    Tissue: Cotyledon and Hypocotyl.
  3. "Nucleotide sequence of glutathione S-transferase cDNA from Arabidopsis thaliana."
    Sopory S.K.
    Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  5. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  7. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  8. "Probing the diversity of the Arabidopsis glutathione S-transferase gene family."
    Wagner U., Edwards R., Dixon D.P., Mauch F.
    Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, GENE FAMILY, NOMENCLATURE.
    Strain: cv. Columbia.

Entry informationi

Entry nameiGSTU5_ARATH
AccessioniPrimary (citable) accession number: P46421
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3