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P46421

- GSTU5_ARATH

UniProt

P46421 - GSTU5_ARATH

Protein

Glutathione S-transferase U5

Gene

GSTU5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    In vitro, possesses glutathione S-transferase activity toward 1-chloro-2,4-dinitrobenzene (CDNB) and benzyl isothiocyanate (BITC). May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides.1 Publication

    Catalytic activityi

    RX + glutathione = HX + R-S-glutathione.

    GO - Molecular functioni

    1. glutathione binding Source: TAIR
    2. glutathione transferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. response to oxidative stress Source: TAIR
    2. response to toxic substance Source: UniProtKB-KW
    3. toxin catabolic process Source: TAIR

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Detoxification, Stress response

    Enzyme and pathway databases

    BioCyciARA:AT2G29450-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase U5 (EC:2.5.1.18)
    Short name:
    AtGSTU5
    Alternative name(s):
    AtGSTU1
    GST class-tau member 5
    Glutathione S-transferase 103-1A
    Gene namesi
    Name:GSTU5
    Synonyms:GSTU1
    Ordered Locus Names:At2g29450
    ORF Names:F16P2.17
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G29450.

    Subcellular locationi

    Cytoplasmcytosol Curated

    GO - Cellular componenti

    1. cytoplasm Source: TAIR
    2. cytosol Source: TAIR
    3. plasma membrane Source: TAIR
    4. plasmodesma Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 224224Glutathione S-transferase U5PRO_0000185860Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei151 – 1511PhosphothreonineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP46421.
    PRIDEiP46421.

    Expressioni

    Inductioni

    By auxin.

    Gene expression databases

    ArrayExpressiP46421.
    GenevestigatoriP46421.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT2G29450.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliP46421.
    SMRiP46421. Positions 5-209.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 8480GST N-terminalAdd
    BLAST
    Domaini89 – 217129GST C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni15 – 162Glutathione bindingBy similarity
    Regioni41 – 422Glutathione bindingBy similarity
    Regioni55 – 562Glutathione bindingBy similarity
    Regioni68 – 692Glutathione bindingBy similarity

    Sequence similaritiesi

    Belongs to the GST superfamily. Tau family.Curated
    Contains 1 GST C-terminal domain.Curated
    Contains 1 GST N-terminal domain.Curated

    Phylogenomic databases

    eggNOGiCOG0625.
    HOGENOMiHOG000125749.
    InParanoidiP46421.
    KOiK00799.
    OMAiERSKARF.
    PhylomeDBiP46421.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF13417. GST_N_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P46421-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEKEEVKLL GIWASPFSRR VEMALKLKGI PYEYVEEILE NKSPLLLALN    50
    PIHKKVPVLV HNGKTILESH VILEYIDETW PQNPILPQDP YERSKARFFA 100
    KLVDEQIMNV GFISMARADE KGREVLAEQV RELIMYLEKE LVGKDYFGGK 150
    TVGFLDFVAG SLIPFCLERG WEGIGLEVIT EEKFPEFKRW VRNLEKVEIV 200
    KDCVPPREEH VEHMNYMAER VRSS 224
    Length:224
    Mass (Da):26,000
    Last modified:November 1, 1995 - v1
    Checksum:iBA2F5C06B94FFCC4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X89216 Genomic DNA. Translation: CAA61504.1.
    U30489 Genomic DNA. Translation: AAA74019.1.
    D44465 mRNA. Translation: BAA07917.1.
    AF144382 mRNA. Translation: AAD34992.1.
    AC004561 Genomic DNA. Translation: AAC95193.1.
    CP002685 Genomic DNA. Translation: AEC08255.1.
    AY062676 mRNA. Translation: AAL32754.1.
    BT001228 mRNA. Translation: AAN65115.1.
    AY088413 mRNA. Translation: AAM65950.1.
    PIRiS66354.
    RefSeqiNP_180506.1. NM_128499.4.
    UniGeneiAt.19941.

    Genome annotation databases

    EnsemblPlantsiAT2G29450.1; AT2G29450.1; AT2G29450.
    GeneIDi817494.
    KEGGiath:AT2G29450.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X89216 Genomic DNA. Translation: CAA61504.1 .
    U30489 Genomic DNA. Translation: AAA74019.1 .
    D44465 mRNA. Translation: BAA07917.1 .
    AF144382 mRNA. Translation: AAD34992.1 .
    AC004561 Genomic DNA. Translation: AAC95193.1 .
    CP002685 Genomic DNA. Translation: AEC08255.1 .
    AY062676 mRNA. Translation: AAL32754.1 .
    BT001228 mRNA. Translation: AAN65115.1 .
    AY088413 mRNA. Translation: AAM65950.1 .
    PIRi S66354.
    RefSeqi NP_180506.1. NM_128499.4.
    UniGenei At.19941.

    3D structure databases

    ProteinModelPortali P46421.
    SMRi P46421. Positions 5-209.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT2G29450.1-P.

    Proteomic databases

    PaxDbi P46421.
    PRIDEi P46421.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G29450.1 ; AT2G29450.1 ; AT2G29450 .
    GeneIDi 817494.
    KEGGi ath:AT2G29450.

    Organism-specific databases

    TAIRi AT2G29450.

    Phylogenomic databases

    eggNOGi COG0625.
    HOGENOMi HOG000125749.
    InParanoidi P46421.
    KOi K00799.
    OMAi ERSKARF.
    PhylomeDBi P46421.

    Enzyme and pathway databases

    BioCyci ARA:AT2G29450-MONOMER.

    Gene expression databases

    ArrayExpressi P46421.
    Genevestigatori P46421.

    Family and domain databases

    Gene3Di 1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProi IPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    Pfami PF13417. GST_N_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEi PS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of an auxin-inducible glutathione S-transferase gene of Arabidopsis thaliana."
      van der Kop D.A.M., Schuyer M., Scheres B., van der Zaal B.J., Hooykaas P.J.J.
      Plant Mol. Biol. 30:839-844(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Landsberg erecta.
    2. "Auxins and their related substances competitively inhibit enzymatic activity of an Arabidopsis glutathione S-transferase isozyme expressed in Escherichia coli."
      Watahiki M., Yamamoto K.
      Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
      Tissue: Cotyledon and Hypocotyl.
    3. "Nucleotide sequence of glutathione S-transferase cDNA from Arabidopsis thaliana."
      Sopory S.K.
      Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    5. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    7. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    8. "Probing the diversity of the Arabidopsis glutathione S-transferase gene family."
      Wagner U., Edwards R., Dixon D.P., Mauch F.
      Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, GENE FAMILY, NOMENCLATURE.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiGSTU5_ARATH
    AccessioniPrimary (citable) accession number: P46421
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3