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P46421 (GSTU5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutathione S-transferase U5

Short name=AtGSTU5
EC=2.5.1.18
Alternative name(s):
AtGSTU1
GST class-tau member 5
Glutathione S-transferase 103-1A
Gene names
Name:GSTU5
Synonyms:GSTU1
Ordered Locus Names:At2g29450
ORF Names:F16P2.17
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length224 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

In vitro, possesses glutathione S-transferase activity toward 1-chloro-2,4-dinitrobenzene (CDNB) and benzyl isothiocyanate (BITC). May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides. Ref.8

Catalytic activity

RX + glutathione = HX + R-S-glutathione.

Subcellular location

Cytoplasmcytosol Probable.

Induction

By auxin.

Sequence similarities

Belongs to the GST superfamily. Tau family.

Contains 1 GST C-terminal domain.

Contains 1 GST N-terminal domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 224224Glutathione S-transferase U5
PRO_0000185860

Regions

Domain5 – 8480GST N-terminal
Domain89 – 217129GST C-terminal
Region15 – 162Glutathione binding By similarity
Region41 – 422Glutathione binding By similarity
Region55 – 562Glutathione binding By similarity
Region68 – 692Glutathione binding By similarity

Amino acid modifications

Modified residue1511Phosphothreonine By similarity

Sequences

Sequence LengthMass (Da)Tools
P46421 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: BA2F5C06B94FFCC4

FASTA22426,000
        10         20         30         40         50         60 
MAEKEEVKLL GIWASPFSRR VEMALKLKGI PYEYVEEILE NKSPLLLALN PIHKKVPVLV 

        70         80         90        100        110        120 
HNGKTILESH VILEYIDETW PQNPILPQDP YERSKARFFA KLVDEQIMNV GFISMARADE 

       130        140        150        160        170        180 
KGREVLAEQV RELIMYLEKE LVGKDYFGGK TVGFLDFVAG SLIPFCLERG WEGIGLEVIT 

       190        200        210        220 
EEKFPEFKRW VRNLEKVEIV KDCVPPREEH VEHMNYMAER VRSS 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of an auxin-inducible glutathione S-transferase gene of Arabidopsis thaliana."
van der Kop D.A.M., Schuyer M., Scheres B., van der Zaal B.J., Hooykaas P.J.J.
Plant Mol. Biol. 30:839-844(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Landsberg erecta.
[2]"Auxins and their related substances competitively inhibit enzymatic activity of an Arabidopsis glutathione S-transferase isozyme expressed in Escherichia coli."
Watahiki M., Yamamoto K.
Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
Tissue: Cotyledon and Hypocotyl.
[3]"Nucleotide sequence of glutathione S-transferase cDNA from Arabidopsis thaliana."
Sopory S.K.
Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[5]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[6]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[7]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]"Probing the diversity of the Arabidopsis glutathione S-transferase gene family."
Wagner U., Edwards R., Dixon D.P., Mauch F.
Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, GENE FAMILY, NOMENCLATURE.
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X89216 Genomic DNA. Translation: CAA61504.1.
U30489 Genomic DNA. Translation: AAA74019.1.
D44465 mRNA. Translation: BAA07917.1.
AF144382 mRNA. Translation: AAD34992.1.
AC004561 Genomic DNA. Translation: AAC95193.1.
CP002685 Genomic DNA. Translation: AEC08255.1.
AY062676 mRNA. Translation: AAL32754.1.
BT001228 mRNA. Translation: AAN65115.1.
AY088413 mRNA. Translation: AAM65950.1.
PIRS66354.
RefSeqNP_180506.1. NM_128499.4.
UniGeneAt.19941.

3D structure databases

ProteinModelPortalP46421.
SMRP46421. Positions 5-209.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT2G29450.1-P.

Proteomic databases

PaxDbP46421.
PRIDEP46421.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G29450.1; AT2G29450.1; AT2G29450.
GeneID817494.
KEGGath:AT2G29450.

Organism-specific databases

TAIRAT2G29450.

Phylogenomic databases

eggNOGCOG0625.
HOGENOMHOG000125749.
InParanoidP46421.
KOK00799.
OMAERSKARF.
PhylomeDBP46421.

Enzyme and pathway databases

BioCycARA:AT2G29450-MONOMER.

Gene expression databases

ArrayExpressP46421.
GenevestigatorP46421.

Family and domain databases

Gene3D1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamPF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSTU5_ARATH
AccessionPrimary (citable) accession number: P46421
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names