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P46420

- GSTF4_MAIZE

UniProt

P46420 - GSTF4_MAIZE

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Protein

Glutathione S-transferase 4

Gene

GST4

Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the detoxification of certain herbicides. Most active with substrates possessing a chloroacetamide structure. Trans-cinnamic acid and 1-chloro-2,4-dinitrobenzene are not effective substrates. May play an important role in the benoxacor-mediated protection of maize from metolachlor injury.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

pH dependencei

Optimum pH is 7.5-8. Active from pH 6 to 9.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei14 – 141GlutathioneBy similarity

GO - Molecular functioni

  1. glutathione transferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase 4 (EC:2.5.1.18)
Alternative name(s):
GST class-phi member 4
GST-27
GST-IV
Gene namesi
Name:GST4
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Organism-specific databases

GrameneiP46420.
MaizeGDBi113242.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 223222Glutathione S-transferase 4PRO_0000185843Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21Blocked amino end (Ala)

Proteomic databases

PRIDEiP46420.

Expressioni

Tissue specificityi

Seedling roots.

Inductioni

By herbicides.

Interactioni

Subunit structurei

Homodimer or heterodimer of GST-I and GST-IV (=GST-II).

Structurei

3D structure databases

ProteinModelPortaliP46420.
SMRiP46420. Positions 6-212.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 8582GST N-terminalAdd
BLAST
Domaini90 – 223134GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni43 – 442Glutathione bindingBy similarity
Regioni56 – 572Glutathione bindingBy similarity
Regioni69 – 702Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the GST superfamily. Phi family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

HOGENOMiHOG000125746.
KOiK00799.
OMAiDAIYEAY.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46420-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATPAVKVYG WAISPFVSRA LLALEEAGVD YELVPMSRQD GDHRRPEHLA
60 70 80 90 100
RNPFGKVPVL EDGDLTLFES RAIARHVLRK HKPELLGGGR LEQTAMVDVW
110 120 130 140 150
LEVEAHQLSP PAIAIVVECV FAPFLGRERN QAVVDENVEK LKKVLEVYEA
160 170 180 190 200
RLATCTYLAG DFLSLADLSP FTIMHCLMAT EYAALVHALP HVSAWWQGLA
210 220
ARPAANKVAQ FMPVGAGAPK EQE
Length:223
Mass (Da):24,570
Last modified:January 23, 2007 - v2
Checksum:i24235245AFB4A403
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79515 mRNA. Translation: CAA56047.1.
U12679 mRNA. Translation: AAA20585.1.
PIRiS52037.
RefSeqiNP_001105366.1. NM_001111896.1.
UniGeneiZm.17839.

Genome annotation databases

GeneIDi542311.
KEGGizma:542311.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79515 mRNA. Translation: CAA56047.1 .
U12679 mRNA. Translation: AAA20585.1 .
PIRi S52037.
RefSeqi NP_001105366.1. NM_001111896.1.
UniGenei Zm.17839.

3D structure databases

ProteinModelPortali P46420.
SMRi P46420. Positions 6-212.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P46420.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 542311.
KEGGi zma:542311.

Organism-specific databases

Gramenei P46420.
MaizeGDBi 113242.

Phylogenomic databases

HOGENOMi HOG000125746.
KOi K00799.
OMAi DAIYEAY.

Family and domain databases

Gene3Di 1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProi IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view ]
Pfami PF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view ]
SUPFAMi SSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEi PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of maize herbicide safener-induced cDNAs encoding subunits of glutathione S-transferase isoforms I, II and IV."
    Jepson I., Lay V.J., Holt D.C., Bright S.W.J., Greenland A.J.
    Plant Mol. Biol. 26:1855-1866(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. UE95.
    Tissue: Seedling root.
  2. "A cDNA clone encoding the 27-kilodalton subunits of glutathione S-transferase IV from Zea mays."
    Irzyk G.P., Potter S., Ward E., Fuerst E.P.
    Plant Physiol. 107:311-312(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Pioneer hybrid 3906.
  3. "Purification and characterization of a glutathione S-transferase from benoxacor-treated maize (Zea mays)."
    Irzyk G.P., Fuerst E.P.
    Plant Physiol. 102:803-810(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.

Entry informationi

Entry nameiGSTF4_MAIZE
AccessioniPrimary (citable) accession number: P46420
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: October 1, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3