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Protein

Glutathione S-transferase 4

Gene

GST4

Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the detoxification of certain herbicides. Most active with substrates possessing a chloroacetamide structure. Trans-cinnamic acid and 1-chloro-2,4-dinitrobenzene are not effective substrates. May play an important role in the benoxacor-mediated protection of maize from metolachlor injury.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

pH dependencei

Optimum pH is 7.5-8. Active from pH 6 to 9.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei14GlutathioneBy similarity1

GO - Molecular functioni

  • glutathione binding Source: AgBase
  • glutathione transferase activity Source: AgBase
  • protein homodimerization activity Source: AgBase

GO - Biological processi

  • response to herbicide Source: AgBase
  • response to xenobiotic stimulus Source: AgBase
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase 4 (EC:2.5.1.18)
Alternative name(s):
GST class-phi member 4
GST-27
GST-IV
Gene namesi
Name:GST4
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeTripsacinaeZea
Proteomesi
  • UP000007305 Componenti: Unplaced

Organism-specific databases

MaizeGDBi113242.

Subcellular locationi

GO - Cellular componenti

  • protein complex Source: AgBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00001858432 – 223Glutathione S-transferase 4Add BLAST222

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2Blocked amino end (Ala)1

Proteomic databases

PaxDbiP46420.
PRIDEiP46420.

Expressioni

Tissue specificityi

Seedling roots.

Inductioni

By herbicides.

Gene expression databases

ExpressionAtlasiP46420. baseline and differential.
GenevisibleiP46420. ZM.

Interactioni

Subunit structurei

Homodimer or heterodimer of GST-I and GST-IV (=GST-II).

GO - Molecular functioni

  • protein homodimerization activity Source: AgBase

Protein-protein interaction databases

STRINGi4577.GRMZM2G132093_P01.

Structurei

3D structure databases

ProteinModelPortaliP46420.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 85GST N-terminalAdd BLAST82
Domaini90 – 223GST C-terminalAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni43 – 44Glutathione bindingBy similarity2
Regioni56 – 57Glutathione bindingBy similarity2
Regioni69 – 70Glutathione bindingBy similarity2

Sequence similaritiesi

Belongs to the GST superfamily. Phi family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiKOG0867. Eukaryota.
COG0625. LUCA.
HOGENOMiHOG000125746.
KOiK00799.
OMAiTPAVKVY.
OrthoDBiEOG09360M18.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46420-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATPAVKVYG WAISPFVSRA LLALEEAGVD YELVPMSRQD GDHRRPEHLA
60 70 80 90 100
RNPFGKVPVL EDGDLTLFES RAIARHVLRK HKPELLGGGR LEQTAMVDVW
110 120 130 140 150
LEVEAHQLSP PAIAIVVECV FAPFLGRERN QAVVDENVEK LKKVLEVYEA
160 170 180 190 200
RLATCTYLAG DFLSLADLSP FTIMHCLMAT EYAALVHALP HVSAWWQGLA
210 220
ARPAANKVAQ FMPVGAGAPK EQE
Length:223
Mass (Da):24,570
Last modified:January 23, 2007 - v2
Checksum:i24235245AFB4A403
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79515 mRNA. Translation: CAA56047.1.
U12679 mRNA. Translation: AAA20585.1.
PIRiS52037.
RefSeqiNP_001105366.1. NM_001111896.2.
UniGeneiZm.17839.

Genome annotation databases

EnsemblPlantsiZm00001d024839_T001; Zm00001d024839_T001; Zm00001d024839.
GeneIDi542311.
GrameneiZm00001d024839_T001; Zm00001d024839_T001; Zm00001d024839.
KEGGizma:542311.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79515 mRNA. Translation: CAA56047.1.
U12679 mRNA. Translation: AAA20585.1.
PIRiS52037.
RefSeqiNP_001105366.1. NM_001111896.2.
UniGeneiZm.17839.

3D structure databases

ProteinModelPortaliP46420.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4577.GRMZM2G132093_P01.

Proteomic databases

PaxDbiP46420.
PRIDEiP46420.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiZm00001d024839_T001; Zm00001d024839_T001; Zm00001d024839.
GeneIDi542311.
GrameneiZm00001d024839_T001; Zm00001d024839_T001; Zm00001d024839.
KEGGizma:542311.

Organism-specific databases

MaizeGDBi113242.

Phylogenomic databases

eggNOGiKOG0867. Eukaryota.
COG0625. LUCA.
HOGENOMiHOG000125746.
KOiK00799.
OMAiTPAVKVY.
OrthoDBiEOG09360M18.

Gene expression databases

ExpressionAtlasiP46420. baseline and differential.
GenevisibleiP46420. ZM.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSTF4_MAIZE
AccessioniPrimary (citable) accession number: P46420
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.