Reviewed, UniProtKB/Swiss-Prot P46420 (GSTF4_MAIZE)

Names and origin

Protein names

Recommended name:
    Glutathione S-transferase 4
    EC=2.5.1.18
Alternative name(s):
    GST-IV
    GST-27
    GST class-phi member 4

Gene names

Name:

GST4

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	223 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the detoxification of certain herbicides. Most active with substrates possessing a chloroacetamide structure. Trans-cinnamic acid and 1-chloro-2,4-dinitrobenzene are not effective substrates. May play an important role in the benoxacor-mediated protection of maize from metolachlor injury.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer or heterodimer of GST-I and GST-IV (=GST-II).
Tissue specificity	Seedling roots.
Induction	By herbicides.
Sequence similarities	Belongs to the GST superfamily. Phi family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.
Biophysicochemical properties	pH dependence: Optimum pH is 7.5-8. Active from pH 6 to 9.

Ontologies

Keywords
Molecular function	Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 223	222	Glutathione S-transferase 4		PRO_0000185843
Regions
	Domain	4 – 85	82	GST N-terminal
	Domain	90 – 223	134	GST C-terminal
Amino acid modifications
	Modified residue	2	1	Blocked amino end (Ala)

Sequences

	Sequence		Length	Mass (Da)
	P46420-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 24235245AFB4A403	FASTA	223	24,570
	10 20 30 40 50 60 MATPAVKVYG WAISPFVSRA LLALEEAGVD YELVPMSRQD GDHRRPEHLA RNPFGKVPVL 70 80 90 100 110 120 EDGDLTLFES RAIARHVLRK HKPELLGGGR LEQTAMVDVW LEVEAHQLSP PAIAIVVECV 130 140 150 160 170 180 FAPFLGRERN QAVVDENVEK LKKVLEVYEA RLATCTYLAG DFLSLADLSP FTIMHCLMAT 190 200 210 220 EYAALVHALP HVSAWWQGLA ARPAANKVAQ FMPVGAGAPK EQE

References

[1]	"Cloning and characterization of maize herbicide safener-induced cDNAs encoding subunits of glutathione S-transferase isoforms I, II and IV." Jepson I., Lay V.J., Holt D.C., Bright S.W.J., Greenland A.J. Plant Mol. Biol. 26:1855-1866(1994) [PubMed: 7858222] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. UE95. Tissue: Seedling root.
[2]	"A cDNA clone encoding the 27-kilodalton subunits of glutathione S-transferase IV from Zea mays." Irzyk G.P., Potter S., Ward E., Fuerst E.P. Plant Physiol. 107:311-312(1995) [PubMed: 7870838] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Pioneer hybrid 3906.
[3]	"Purification and characterization of a glutathione S-transferase from benoxacor-treated maize (Zea mays)." Irzyk G.P., Fuerst E.P. Plant Physiol. 102:803-810(1993) [PubMed: 8278534] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X79515 mRNA. Translation: CAA56047.1. U12679 mRNA. Translation: AAA20585.1.
PIR	S52037.
RefSeq	NP_001105366.1.
UniGene	Zm.94411
3D structure databases
HSSP	HSSP built from PDB template 1AXD based on UniProtKB P12653.
ModBase	Search...
Genome annotation databases
GeneID	542311.
Organism-specific databases
Gramene	P46420.
MaizeGDB	113242.
Enzyme and pathway databases
BRENDA	2.5.1.18. 289.
Family and domain databases
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:1.20.1050.10. GST_C_like. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTF4_MAIZE

Accession

Primary (citable) accession number: P46420

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	March 3, 2009
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)