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P46420

- GSTF4_MAIZE

UniProt

P46420 - GSTF4_MAIZE

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Protein
Glutathione S-transferase 4
Gene
GST4
Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the detoxification of certain herbicides. Most active with substrates possessing a chloroacetamide structure. Trans-cinnamic acid and 1-chloro-2,4-dinitrobenzene are not effective substrates. May play an important role in the benoxacor-mediated protection of maize from metolachlor injury.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

pH dependencei

Optimum pH is 7.5-8. Active from pH 6 to 9.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei14 – 141Glutathione By similarity

GO - Molecular functioni

  1. glutathione transferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase 4 (EC:2.5.1.18)
Alternative name(s):
GST class-phi member 4
GST-27
GST-IV
Gene namesi
Name:GST4
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Organism-specific databases

GrameneiP46420.
MaizeGDBi113242.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 223222Glutathione S-transferase 4
PRO_0000185843Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21Blocked amino end (Ala)

Proteomic databases

PRIDEiP46420.

Expressioni

Tissue specificityi

Seedling roots.

Inductioni

By herbicides.

Interactioni

Subunit structurei

Homodimer or heterodimer of GST-I and GST-IV (=GST-II).

Structurei

3D structure databases

ProteinModelPortaliP46420.
SMRiP46420. Positions 6-212.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 8582GST N-terminal
Add
BLAST
Domaini90 – 223134GST C-terminal
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni43 – 442Glutathione binding By similarity
Regioni56 – 572Glutathione binding By similarity
Regioni69 – 702Glutathione binding By similarity

Sequence similaritiesi

Belongs to the GST superfamily. Phi family.

Phylogenomic databases

HOGENOMiHOG000125746.
KOiK00799.
OMAiDAIYEAY.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46420-1 [UniParc]FASTAAdd to Basket

« Hide

MATPAVKVYG WAISPFVSRA LLALEEAGVD YELVPMSRQD GDHRRPEHLA    50
RNPFGKVPVL EDGDLTLFES RAIARHVLRK HKPELLGGGR LEQTAMVDVW 100
LEVEAHQLSP PAIAIVVECV FAPFLGRERN QAVVDENVEK LKKVLEVYEA 150
RLATCTYLAG DFLSLADLSP FTIMHCLMAT EYAALVHALP HVSAWWQGLA 200
ARPAANKVAQ FMPVGAGAPK EQE 223
Length:223
Mass (Da):24,570
Last modified:January 23, 2007 - v2
Checksum:i24235245AFB4A403
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X79515 mRNA. Translation: CAA56047.1.
U12679 mRNA. Translation: AAA20585.1.
PIRiS52037.
RefSeqiNP_001105366.1. NM_001111896.1.
UniGeneiZm.17839.

Genome annotation databases

GeneIDi542311.
KEGGizma:542311.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X79515 mRNA. Translation: CAA56047.1 .
U12679 mRNA. Translation: AAA20585.1 .
PIRi S52037.
RefSeqi NP_001105366.1. NM_001111896.1.
UniGenei Zm.17839.

3D structure databases

ProteinModelPortali P46420.
SMRi P46420. Positions 6-212.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P46420.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 542311.
KEGGi zma:542311.

Organism-specific databases

Gramenei P46420.
MaizeGDBi 113242.

Phylogenomic databases

HOGENOMi HOG000125746.
KOi K00799.
OMAi DAIYEAY.

Family and domain databases

Gene3Di 1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProi IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view ]
Pfami PF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view ]
SUPFAMi SSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEi PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of maize herbicide safener-induced cDNAs encoding subunits of glutathione S-transferase isoforms I, II and IV."
    Jepson I., Lay V.J., Holt D.C., Bright S.W.J., Greenland A.J.
    Plant Mol. Biol. 26:1855-1866(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. UE95.
    Tissue: Seedling root.
  2. "A cDNA clone encoding the 27-kilodalton subunits of glutathione S-transferase IV from Zea mays."
    Irzyk G.P., Potter S., Ward E., Fuerst E.P.
    Plant Physiol. 107:311-312(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Pioneer hybrid 3906.
  3. "Purification and characterization of a glutathione S-transferase from benoxacor-treated maize (Zea mays)."
    Irzyk G.P., Fuerst E.P.
    Plant Physiol. 102:803-810(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.

Entry informationi

Entry nameiGSTF4_MAIZE
AccessioniPrimary (citable) accession number: P46420
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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