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P46420 (GSTF4_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione S-transferase 4

EC=2.5.1.18
Alternative name(s):
GST class-phi member 4
GST-27
GST-IV
Gene names
Name:GST4
OrganismZea mays (Maize)
Taxonomic identifier4577 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Protein attributes

Sequence length223 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the detoxification of certain herbicides. Most active with substrates possessing a chloroacetamide structure. Trans-cinnamic acid and 1-chloro-2,4-dinitrobenzene are not effective substrates. May play an important role in the benoxacor-mediated protection of maize from metolachlor injury.

Catalytic activity

RX + glutathione = HX + R-S-glutathione.

Subunit structure

Homodimer or heterodimer of GST-I and GST-IV (=GST-II).

Tissue specificity

Seedling roots.

Induction

By herbicides.

Sequence similarities

Belongs to the GST superfamily. Phi family.

Contains 1 GST C-terminal domain.

Contains 1 GST N-terminal domain.

Biophysicochemical properties

pH dependence:

Optimum pH is 7.5-8. Active from pH 6 to 9.

Ontologies

Keywords
   Molecular functionTransferase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Molecular_functionglutathione transferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 223222Glutathione S-transferase 4
PRO_0000185843

Regions

Domain4 – 8582GST N-terminal
Domain90 – 223134GST C-terminal
Region43 – 442Glutathione binding By similarity
Region56 – 572Glutathione binding By similarity
Region69 – 702Glutathione binding By similarity

Sites

Binding site141Glutathione By similarity

Amino acid modifications

Modified residue21Blocked amino end (Ala)

Sequences

Sequence LengthMass (Da)Tools
P46420 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 24235245AFB4A403

FASTA22324,570
        10         20         30         40         50         60 
MATPAVKVYG WAISPFVSRA LLALEEAGVD YELVPMSRQD GDHRRPEHLA RNPFGKVPVL 

        70         80         90        100        110        120 
EDGDLTLFES RAIARHVLRK HKPELLGGGR LEQTAMVDVW LEVEAHQLSP PAIAIVVECV 

       130        140        150        160        170        180 
FAPFLGRERN QAVVDENVEK LKKVLEVYEA RLATCTYLAG DFLSLADLSP FTIMHCLMAT 

       190        200        210        220 
EYAALVHALP HVSAWWQGLA ARPAANKVAQ FMPVGAGAPK EQE 

« Hide

References

[1]"Cloning and characterization of maize herbicide safener-induced cDNAs encoding subunits of glutathione S-transferase isoforms I, II and IV."
Jepson I., Lay V.J., Holt D.C., Bright S.W.J., Greenland A.J.
Plant Mol. Biol. 26:1855-1866(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. UE95.
Tissue: Seedling root.
[2]"A cDNA clone encoding the 27-kilodalton subunits of glutathione S-transferase IV from Zea mays."
Irzyk G.P., Potter S., Ward E., Fuerst E.P.
Plant Physiol. 107:311-312(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Pioneer hybrid 3906.
[3]"Purification and characterization of a glutathione S-transferase from benoxacor-treated maize (Zea mays)."
Irzyk G.P., Fuerst E.P.
Plant Physiol. 102:803-810(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X79515 mRNA. Translation: CAA56047.1.
U12679 mRNA. Translation: AAA20585.1.
PIRS52037.
RefSeqNP_001105366.1. NM_001111896.1.
UniGeneZm.17839.

3D structure databases

ProteinModelPortalP46420.
SMRP46420. Positions 6-212.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP46420.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID542311.
KEGGzma:542311.

Organism-specific databases

GrameneP46420.
MaizeGDB113242.

Phylogenomic databases

HOGENOMHOG000125746.
OMADAIYEAY.

Family and domain databases

Gene3D1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSTF4_MAIZE
AccessionPrimary (citable) accession number: P46420
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families