Reviewed, UniProtKB/Swiss-Prot P46418 (GSTA5_RAT)

Names and origin

Protein names

Recommended name:
    Glutathione S-transferase alpha-5
    EC=2.5.1.18
Alternative name(s):
    Glutathione S-transferase Yc-2
      Short name=GST Yc2
    GST A5-5

Gene names

Name:	Gsta5
Synonyms:	Gstyc2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has substantial activity toward aflatoxin B1-8,9-epoxide.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Heterodimer of YC1 and YC2.
Subcellular location	Cytoplasm.
Tissue specificity	Liver, nasal mucosa and epididymis.
Developmental stage	Liver from adult female rats contains about 10-fold greater levels of YC2 than is found in liver from adult male rats.
Induction	By ethoxyquin, oltipraz, butylated hydroxyanisole, and phenobarbitol.
Sequence similarities	Belongs to the GST superfamily. Alpha family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	response to drug Non-traceable author statement. Source: RGD xenobiotic catabolic process Inferred from direct assay. Source: RGD
Cellular component	cytosol Inferred from direct assay. Source: RGD nuclear outer membrane Inferred from direct assay. Source: RGD
Molecular function	drug binding Inferred from direct assay. Source: RGD glutathione binding Inferred from direct assay. Source: RGD glutathione transferase activity Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 221	220	Glutathione S-transferase alpha-5		PRO_0000185796
Regions
	Domain	3 – 83	81	GST N-terminal
	Domain	85 – 207	123	GST C-terminal

Sequences

	Sequence		Length	Mass (Da)
	P46418-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: EEDE9873765BFDB5	FASTA	221	25,347
	10 20 30 40 50 60 MPGKPVLHYF DGRGRMEPIR WLLAAAGVEF EENFLKTRDD LARLRSDGSL MFEQVPMVEI 70 80 90 100 110 120 DGMKLVQTKA ILNYIATKYN LYGKDMKERA LIDMYAEGVA DLELMVLYYP YMPPGEKEAS 130 140 150 160 170 180 LAKIKDKARN RYFPAYEKVL KSHGQDYLVG NKLSRADVSL VELLYHVEEM DPGIVDNFPL 190 200 210 220 LKALRTRVSN LPTVKKFLQP GSQRKPFDDE KCVESAKKIF S

References

[1]	"Cloning of cDNAs from fetal rat liver encoding glutathione S-transferase Yc polypeptides. The Yc2 subunit is expressed in adult rat liver resistant to the hepatocarcinogen aflatoxin B1." Hayes J.D., Nguyen T., Judah D.J., Petersson D.G., Neal G.E. J. Biol. Chem. 269:20707-20717(1994) [PubMed: 8051171] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Fischer 344. Tissue: Liver.
[2]	"Characterization of the rat glutathione S-transferase Yc2 subunit gene, GSTA5: identification of a putative antioxidant-responsive element in the 5'-flanking region of rat GSTA5 that may mediate chemoprotection against aflatoxin B1." Pulford D.J., Hayes J.D. Biochem. J. 318:75-84(1996) [PubMed: 8761455] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Ethoxyquin-induced resistance to aflatoxin B1 in the rat is associated with the expression of a novel alpha-class glutathione S-transferase subunit, Yc2, which possesses high catalytic activity for aflatoxin B1-8,9-epoxide." Hayes J.D., Judah D.J., McLellan L.I., Kerr L.A., Peacock S.D., Neal G.E. Biochem. J. 279:385-398(1991) [PubMed: 1953636] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE. Strain: Fischer 344. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X78847 mRNA. Translation: CAA55404.1. S72506 mRNA. Translation: AAP21065.1. S82820 mRNA. Translation: AAB46796.1.
IPI	IPI00211897.
PIR	A54858.
RefSeq	NP_001009920.1.
UniGene	Rn.120929
3D structure databases
HSSP	HSSP built from PDB template 1K3O based on UniProtKB P08263.
SMR	P46418. Positions 4-220.
ModBase	Search...
Proteomic databases
PRIDE	P46418.
Genome annotation databases
Ensembl	ENSRNOG00000029711. Rattus norvegicus. [Contig view]
GeneID	494500.
KEGG	rno:494500.
NMPDR	fig|10116.3.peg.29792.
Organism-specific databases
RGD	2753. Gsta5.
Phylogenomic databases
HOVERGEN	P46418.
OMA	P46418. KCVESAK.
Enzyme and pathway databases
BRENDA	2.5.1.18. 248.
Gene expression databases
ArrayExpress	P46418.
GermOnline	ENSRNOG00000029711. Rattus norvegicus.
Family and domain databases
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR003080. GST_alpha. IPR004046. GST_C. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:1.20.1050.10. GST_C_like. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PANTHER	PTHR11571:SF4. GST_alpha. 1 hit.
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PRINTS	PR01266. GSTRNSFRASEA.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	697639.

Entry information

Entry name

GSTA5_RAT

Accession

Primary (citable) accession number: P46418
Secondary accession number(s): Q6LD91

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)