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P46418

- GSTA5_RAT

UniProt

P46418 - GSTA5_RAT

Protein

Glutathione S-transferase alpha-5

Gene

Gsta5

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has substantial activity toward aflatoxin B1-8,9-epoxide.

    Catalytic activityi

    RX + glutathione = HX + R-S-glutathione.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei9 – 91GlutathioneBy similarity
    Binding sitei45 – 451GlutathioneBy similarity

    GO - Molecular functioni

    1. drug binding Source: RGD
    2. glutathione binding Source: RGD
    3. glutathione transferase activity Source: RGD

    GO - Biological processi

    1. aging Source: RGD
    2. response to drug Source: RGD
    3. response to nutrient levels Source: RGD
    4. xenobiotic catabolic process Source: RGD

    Keywords - Molecular functioni

    Transferase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase alpha-5 (EC:2.5.1.18)
    Alternative name(s):
    GST A5-5
    Glutathione S-transferase Yc-2
    Short name:
    GST Yc2
    Gene namesi
    Name:Gsta5
    Synonyms:Gstyc2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 9

    Organism-specific databases

    RGDi2753. Gsta5.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: RGD
    2. nuclear outer membrane Source: RGD
    3. nucleus Source: RGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 221220Glutathione S-transferase alpha-5PRO_0000185796Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei4 – 41N6-succinyllysineBy similarity

    Proteomic databases

    PaxDbiP46418.
    PRIDEiP46418.

    Expressioni

    Tissue specificityi

    Liver, nasal mucosa and epididymis.

    Developmental stagei

    Liver from adult female rats contains about 10-fold greater levels of YC2 than is found in liver from adult male rats.

    Inductioni

    By ethoxyquin, oltipraz, butylated hydroxyanisole, and phenobarbitol.

    Gene expression databases

    GenevestigatoriP46418.

    Interactioni

    Subunit structurei

    Heterodimer of YC1 and YC2.

    Structurei

    3D structure databases

    ProteinModelPortaliP46418.
    SMRiP46418. Positions 4-220.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 8381GST N-terminalAdd
    BLAST
    Domaini85 – 207123GST C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni54 – 552Glutathione bindingBy similarity
    Regioni67 – 682Glutathione bindingBy similarity

    Sequence similaritiesi

    Belongs to the GST superfamily. Alpha family.Curated
    Contains 1 GST C-terminal domain.Curated
    Contains 1 GST N-terminal domain.Curated

    Phylogenomic databases

    eggNOGiNOG266414.
    GeneTreeiENSGT00670000097856.
    HOGENOMiHOG000115734.
    HOVERGENiHBG053749.
    InParanoidiP46418.
    KOiK00799.
    OMAiKDIPRLM.
    OrthoDBiEOG79CZ0K.
    PhylomeDBiP46418.
    TreeFamiTF105321.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR003080. GST_alpha.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view]
    PRINTSiPR01266. GSTRNSFRASEA.
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P46418-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPGKPVLHYF DGRGRMEPIR WLLAAAGVEF EENFLKTRDD LARLRSDGSL    50
    MFEQVPMVEI DGMKLVQTKA ILNYIATKYN LYGKDMKERA LIDMYAEGVA 100
    DLELMVLYYP YMPPGEKEAS LAKIKDKARN RYFPAYEKVL KSHGQDYLVG 150
    NKLSRADVSL VELLYHVEEM DPGIVDNFPL LKALRTRVSN LPTVKKFLQP 200
    GSQRKPFDDE KCVESAKKIF S 221
    Length:221
    Mass (Da):25,347
    Last modified:January 23, 2007 - v2
    Checksum:iEEDE9873765BFDB5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78847 mRNA. Translation: CAA55404.1.
    S72506 mRNA. Translation: AAP21065.1.
    S82820 mRNA. Translation: AAB46796.1.
    PIRiA54858.
    RefSeqiNP_001009920.1. NM_001009920.2.
    NP_001153211.1. NM_001159739.2.
    UniGeneiRn.120929.

    Genome annotation databases

    EnsembliENSRNOT00000040950; ENSRNOP00000042770; ENSRNOG00000029711.
    GeneIDi494500.
    KEGGirno:494500.
    UCSCiRGD:2753. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78847 mRNA. Translation: CAA55404.1 .
    S72506 mRNA. Translation: AAP21065.1 .
    S82820 mRNA. Translation: AAB46796.1 .
    PIRi A54858.
    RefSeqi NP_001009920.1. NM_001009920.2.
    NP_001153211.1. NM_001159739.2.
    UniGenei Rn.120929.

    3D structure databases

    ProteinModelPortali P46418.
    SMRi P46418. Positions 4-220.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P46418.
    PRIDEi P46418.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000040950 ; ENSRNOP00000042770 ; ENSRNOG00000029711 .
    GeneIDi 494500.
    KEGGi rno:494500.
    UCSCi RGD:2753. rat.

    Organism-specific databases

    CTDi 2940.
    RGDi 2753. Gsta5.

    Phylogenomic databases

    eggNOGi NOG266414.
    GeneTreei ENSGT00670000097856.
    HOGENOMi HOG000115734.
    HOVERGENi HBG053749.
    InParanoidi P46418.
    KOi K00799.
    OMAi KDIPRLM.
    OrthoDBi EOG79CZ0K.
    PhylomeDBi P46418.
    TreeFami TF105321.

    Miscellaneous databases

    NextBioi 697639.
    PROi P46418.

    Gene expression databases

    Genevestigatori P46418.

    Family and domain databases

    Gene3Di 1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProi IPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR003080. GST_alpha.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    Pfami PF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view ]
    PRINTSi PR01266. GSTRNSFRASEA.
    SUPFAMi SSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEi PS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of cDNAs from fetal rat liver encoding glutathione S-transferase Yc polypeptides. The Yc2 subunit is expressed in adult rat liver resistant to the hepatocarcinogen aflatoxin B1."
      Hayes J.D., Nguyen T., Judah D.J., Petersson D.G., Neal G.E.
      J. Biol. Chem. 269:20707-20717(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Fischer 344.
      Tissue: Liver.
    2. "Characterization of the rat glutathione S-transferase Yc2 subunit gene, GSTA5: identification of a putative antioxidant-responsive element in the 5'-flanking region of rat GSTA5 that may mediate chemoprotection against aflatoxin B1."
      Pulford D.J., Hayes J.D.
      Biochem. J. 318:75-84(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Ethoxyquin-induced resistance to aflatoxin B1 in the rat is associated with the expression of a novel alpha-class glutathione S-transferase subunit, Yc2, which possesses high catalytic activity for aflatoxin B1-8,9-epoxide."
      Hayes J.D., Judah D.J., McLellan L.I., Kerr L.A., Peacock S.D., Neal G.E.
      Biochem. J. 279:385-398(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE.
      Strain: Fischer 344.
      Tissue: Liver.

    Entry informationi

    Entry nameiGSTA5_RAT
    AccessioniPrimary (citable) accession number: P46418
    Secondary accession number(s): Q6LD91
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 110 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3