P46418 (GSTA5_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase alpha-5
EC=2.5.1.18

Alternative name(s):
GST A5-5
Glutathione S-transferase Yc-2
Short name=GST Yc2

Gene names

Name:	Gsta5
Synonyms:	Gstyc2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has substantial activity toward aflatoxin B1-8,9-epoxide.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Heterodimer of YC1 and YC2.
Subcellular location	Cytoplasm.
Tissue specificity	Liver, nasal mucosa and epididymis.
Developmental stage	Liver from adult female rats contains about 10-fold greater levels of YC2 than is found in liver from adult male rats.
Induction	By ethoxyquin, oltipraz, butylated hydroxyanisole, and phenobarbitol.
Sequence similarities	Belongs to the GST superfamily. Alpha family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	aging Inferred from expression pattern PubMed 15967433. Source: RGD response to drug Non-traceable author statement PubMed 10215608. Source: RGD response to nutrient levels Inferred from expression pattern PubMed 19191009. Source: RGD xenobiotic catabolic process Inferred from direct assay PubMed 17197701. Source: RGD
Cellular_component	cytosol Inferred from direct assay PubMed 17197701. Source: RGD nuclear outer membrane Inferred from direct assay PubMed 17197701. Source: RGD
Molecular_function	drug binding Inferred from direct assay PubMed 17197701. Source: RGD glutathione binding Inferred from direct assay PubMed 17197701. Source: RGD glutathione transferase activity Inferred from direct assay PubMed 17197701. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 221	221	Glutathione S-transferase alpha-5		PRO_0000185796
Regions
	Domain	3 – 83	81	GST N-terminal
	Domain	85 – 207	123	GST C-terminal
	Region	54 – 55	2	Glutathione binding By similarity
	Region	67 – 68	2	Glutathione binding By similarity
Sites
	Binding site	9	1	Glutathione By similarity
	Binding site	45	1	Glutathione By similarity

Sequences

	Sequence		Length	Mass (Da)
	P46418 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: EEDE9873765BFDB5	FASTA	221	25,347
	10 20 30 40 50 60 MPGKPVLHYF DGRGRMEPIR WLLAAAGVEF EENFLKTRDD LARLRSDGSL MFEQVPMVEI 70 80 90 100 110 120 DGMKLVQTKA ILNYIATKYN LYGKDMKERA LIDMYAEGVA DLELMVLYYP YMPPGEKEAS 130 140 150 160 170 180 LAKIKDKARN RYFPAYEKVL KSHGQDYLVG NKLSRADVSL VELLYHVEEM DPGIVDNFPL 190 200 210 220 LKALRTRVSN LPTVKKFLQP GSQRKPFDDE KCVESAKKIF S

References

[1]	"Cloning of cDNAs from fetal rat liver encoding glutathione S-transferase Yc polypeptides. The Yc2 subunit is expressed in adult rat liver resistant to the hepatocarcinogen aflatoxin B1." Hayes J.D., Nguyen T., Judah D.J., Petersson D.G., Neal G.E. J. Biol. Chem. 269:20707-20717(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Fischer 344. Tissue: Liver.
[2]	"Characterization of the rat glutathione S-transferase Yc2 subunit gene, GSTA5: identification of a putative antioxidant-responsive element in the 5'-flanking region of rat GSTA5 that may mediate chemoprotection against aflatoxin B1." Pulford D.J., Hayes J.D. Biochem. J. 318:75-84(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Ethoxyquin-induced resistance to aflatoxin B1 in the rat is associated with the expression of a novel alpha-class glutathione S-transferase subunit, Yc2, which possesses high catalytic activity for aflatoxin B1-8,9-epoxide." Hayes J.D., Judah D.J., McLellan L.I., Kerr L.A., Peacock S.D., Neal G.E. Biochem. J. 279:385-398(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE. Strain: Fischer 344. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78847 mRNA. Translation: CAA55404.1. S72506 mRNA. Translation: AAP21065.1. S82820 mRNA. Translation: AAB46796.1.
IPI	IPI00211897.
PIR	A54858.
RefSeq	NP_001009920.1. NM_001009920.1. NP_001153211.1. NM_001159739.1.
UniGene	Rn.120929.
3D structure databases
ProteinModelPortal	P46418.
SMR	P46418. Positions 4-220.
ModBase	Search...
Proteomic databases
PaxDb	P46418.
PRIDE	P46418.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000040950; ENSRNOP00000042770; ENSRNOG00000029711.
GeneID	494500.
KEGG	rno:494500.
UCSC	RGD:2753. rat.
Organism-specific databases
RGD	2753. Gsta5.
Phylogenomic databases
eggNOG	NOG266414.
GeneTree	ENSGT00670000097856.
HOGENOM	HOG000115734.
HOVERGEN	HBG053749.
InParanoid	P46418.
KO	K00799.
OMA	LEIMILY.
OrthoDB	EOG4THVV1.
Gene expression databases
ArrayExpress	P46418.
Genevestigator	P46418.
GermOnline	ENSRNOG00000029711. Rattus norvegicus.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR003080. GST_alpha. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
PANTHER	PTHR11571:SF4. PTHR11571:SF4. 1 hit.
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PRINTS	PR01266. GSTRNSFRASEA.
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	697639.

Entry information

Entry name

GSTA5_RAT

Accession

Primary (citable) accession number: P46418
Secondary accession number(s): Q6LD91

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program