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Protein

Glutathione S-transferase alpha-5

Gene

Gsta5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has substantial activity toward aflatoxin B1-8,9-epoxide.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9GlutathioneBy similarity1
Binding sitei45GlutathioneBy similarity1

GO - Molecular functioni

  • drug binding Source: RGD
  • glutathione binding Source: RGD
  • glutathione transferase activity Source: RGD

GO - Biological processi

  • aging Source: RGD
  • response to drug Source: RGD
  • response to nutrient levels Source: RGD
  • xenobiotic catabolic process Source: RGD

Keywordsi

Molecular functionTransferase

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase alpha-5 (EC:2.5.1.18)
Alternative name(s):
GST A5-5
Glutathione S-transferase Yc-2
Short name:
GST Yc2
Gene namesi
Name:Gsta5
Synonyms:Gstyc2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2753. Gsta5.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001857961 – 221Glutathione S-transferase alpha-5Add BLAST221

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4N6-succinyllysineBy similarity1

Proteomic databases

PaxDbiP46418.
PRIDEiP46418.

PTM databases

iPTMnetiP46418.
PhosphoSitePlusiP46418.

Expressioni

Tissue specificityi

Liver, nasal mucosa and epididymis.

Developmental stagei

Liver from adult female rats contains about 10-fold greater levels of YC2 than is found in liver from adult male rats.

Inductioni

By ethoxyquin, oltipraz, butylated hydroxyanisole, and phenobarbitol.

Interactioni

Subunit structurei

Heterodimer of YC1 and YC2.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000042770.

Structurei

3D structure databases

ProteinModelPortaliP46418.
SMRiP46418.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 83GST N-terminalAdd BLAST81
Domaini85 – 207GST C-terminalAdd BLAST123

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni54 – 55Glutathione bindingBy similarity2
Regioni67 – 68Glutathione bindingBy similarity2

Sequence similaritiesi

Belongs to the GST superfamily. Alpha family.Curated

Phylogenomic databases

eggNOGiKOG1695. Eukaryota.
ENOG4111VAU. LUCA.
HOGENOMiHOG000115734.
HOVERGENiHBG053749.
KOiK00799.
PhylomeDBiP46418.
TreeFamiTF105321.

Family and domain databases

InterProiView protein in InterPro
IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
PfamiView protein in Pfam
PF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
PRINTSiPR01266. GSTRNSFRASEA.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiView protein in PROSITE
PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.

Sequencei

Sequence statusi: Complete.

P46418-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGKPVLHYF DGRGRMEPIR WLLAAAGVEF EENFLKTRDD LARLRSDGSL
60 70 80 90 100
MFEQVPMVEI DGMKLVQTKA ILNYIATKYN LYGKDMKERA LIDMYAEGVA
110 120 130 140 150
DLELMVLYYP YMPPGEKEAS LAKIKDKARN RYFPAYEKVL KSHGQDYLVG
160 170 180 190 200
NKLSRADVSL VELLYHVEEM DPGIVDNFPL LKALRTRVSN LPTVKKFLQP
210 220
GSQRKPFDDE KCVESAKKIF S
Length:221
Mass (Da):25,347
Last modified:January 23, 2007 - v2
Checksum:iEEDE9873765BFDB5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78847 mRNA. Translation: CAA55404.1.
S72506 mRNA. Translation: AAP21065.1.
S82820 mRNA. Translation: AAB46796.1.
PIRiA54858.
RefSeqiNP_001009920.1. NM_001009920.2.
NP_001153211.1. NM_001159739.2.
UniGeneiRn.120929.

Genome annotation databases

GeneIDi494500.
KEGGirno:494500.
UCSCiRGD:2753. rat.

Similar proteinsi

Entry informationi

Entry nameiGSTA5_RAT
AccessioniPrimary (citable) accession number: P46418
Secondary accession number(s): Q6LD91
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: August 30, 2017
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families