Reviewed, UniProtKB/Swiss-Prot P46417 (GSTX3_SOYBN)

Names and origin

Protein names

Recommended name:
    Glutathione S-transferase 3
    EC=2.5.1.18

Gene names

Name:

GST3

Organism

Glycine max (Soybean)

Taxonomic identifier

3847 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the detoxification of certain herbicides.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer. degradation; (R)-lactate from methylglyoxal: step 1/2.
Sequence similarities	Belongs to the GST superfamily. HSP26 family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.
Caution	Was originally (Ref.1) thought to be a glyoxalase I.

Ontologies

Keywords
Molecular function	Transferase
Gene Ontology (GO)
Molecular function	lactoylglutathione lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 219	219	Glutathione S-transferase 3		PRO_0000185873
Regions
	Domain	3 – 82	80	GST N-terminal
	Domain	88 – 216	129	GST C-terminal

Sequences

	Sequence		Length	Mass (Da)
	P46417-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 6A2E46B759476A8C	FASTA	219	25,901
	10 20 30 40 50 60 MSDEVVLLDT WASMYGMRAR IALAEKGVRY EYKEENLMNR SPLLLQMNPI HKKIPVLIHN 70 80 90 100 110 120 GKPICESAII VQYIDEVWND KSPLMPSDPY KRSQARFWVD YIDKKIYDTW KKMWLSKGEE 130 140 150 160 170 180 HEEGKKELIS IFKQLEETLT DKPFYGDDTF GFVDLCLITF SSWFYTYETY GNFKMEEECP 190 200 210 KLMAWVKRCM ERETVSNTLP DAKKVYGLIV ELQKTLESK

References

[1]	Koellner B., Finkelnburg B., Mayerbacher R., Paulus C., Springer B. Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.
[2]	"Cloning and characterization of glyoxalase I from soybean." Skipsey M., Andrews C.J., Townson J.K., Jepson I., Edwards R. Arch. Biochem. Biophys. 374:261-268(2000) [PubMed: 10666306] [Abstract] Cited for: CATALYTIC ACTIVITY, SUBUNIT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X68819 mRNA. Translation: CAA48717.1.
PIR	S47177.
UniGene	Gma.1560
3D structure databases
SMR	P46417. Positions 3-215.
ModBase	Search...
Enzyme and pathway databases
BRENDA	4.4.1.5. 299.
Gene expression databases
Genevestigator	P46417.
Family and domain databases
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:1.20.1050.10. GST_C_like. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTX3_SOYBN

Accession

Primary (citable) accession number: P46417

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	February 9, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)