P46417 (GSTX3_SOYBN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase 3
EC=2.5.1.18

Gene names

Name:

GST3

Organism

Glycine max (Soybean) (Glycine hispida) [Reference proteome]

Taxonomic identifier

3847 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the detoxification of certain herbicides.
Catalytic activity	RX + glutathione = HX + R-S-glutathione. Ref.2
Subunit structure	Homodimer. degradation; (R)-lactate from methylglyoxal: step 1/2. Ref.2
Sequence similarities	Belongs to the GST superfamily. HSP26 family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.
Caution	Was originally (Ref.1) thought to be a glyoxalase I.

Ontologies

Keywords
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 219	219	Glutathione S-transferase 3		PRO_0000185873
Regions
	Domain	3 – 82	80	GST N-terminal
	Domain	88 – 216	129	GST C-terminal
	Region	66 – 67	2	Glutathione binding By similarity
Sites
	Binding site	13	1	Glutathione By similarity
	Binding site	54	1	Glutathione; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P46417 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 6A2E46B759476A8C	FASTA	219	25,901
	10 20 30 40 50 60 MSDEVVLLDT WASMYGMRAR IALAEKGVRY EYKEENLMNR SPLLLQMNPI HKKIPVLIHN 70 80 90 100 110 120 GKPICESAII VQYIDEVWND KSPLMPSDPY KRSQARFWVD YIDKKIYDTW KKMWLSKGEE 130 140 150 160 170 180 HEEGKKELIS IFKQLEETLT DKPFYGDDTF GFVDLCLITF SSWFYTYETY GNFKMEEECP 190 200 210 KLMAWVKRCM ERETVSNTLP DAKKVYGLIV ELQKTLESK

References

[1]	Koellner B., Finkelnburg B., Mayerbacher R., Paulus C., Springer B. Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.
[2]	"Cloning and characterization of glyoxalase I from soybean." Skipsey M., Andrews C.J., Townson J.K., Jepson I., Edwards R. Arch. Biochem. Biophys. 374:261-268(2000) [PubMed] [Europe PMC] [Abstract] Cited for: CATALYTIC ACTIVITY, SUBUNIT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X68819 mRNA. Translation: CAA48717.1.
PIR	S47177.
RefSeq	NP_001236181.1. NM_001249252.2.
UniGene	Gma.1560.
3D structure databases
ProteinModelPortal	P46417.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	GLYMA15G40290.1; GLYMA15G40290.1; GLYMA15G40290.
GeneID	547925.
KEGG	gmx:547925.
Gene expression databases
Genevestigator	P46417.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTX3_SOYBN

Accession

Primary (citable) accession number: P46417

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	March 6, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program