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P46416

- GSHB_ARATH

UniProt

P46416 - GSHB_ARATH

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Protein
Glutathione synthetase, chloroplastic
Gene
GSH2, GSHII, At5g27380, F21A20_90
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Cofactori

Binds 1 magnesium ion per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei193 – 1931Substrate By similarity
Metal bindingi209 – 2091Magnesium By similarity
Binding sitei209 – 2091ATP By similarity
Metal bindingi211 – 2111Magnesium By similarity
Binding sitei287 – 2871Substrate By similarity
Binding sitei374 – 3741ATP By similarity
Metal bindingi432 – 4321Magnesium By similarity
Binding sitei439 – 4391ATP By similarity
Binding sitei490 – 4901ATP By similarity
Binding sitei515 – 5151Substrate By similarity
Binding sitei517 – 5171ATP By similarity
Binding sitei523 – 5231ATP; via carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi428 – 43710ATP By similarity
Nucleotide bindingi464 – 4674ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. glutathione binding Source: UniProtKB
  3. glutathione synthase activity Source: TAIR
  4. magnesium ion binding Source: UniProtKB
  5. protein homodimerization activity Source: UniProtKB

GO - Biological processi

  1. glutathione biosynthetic process Source: TAIR
  2. response to jasmonic acid Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G27380-MONOMER.
UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetase, chloroplastic (EC:6.3.2.3)
Short name:
GSH synthetase
Short name:
GSH-S
Short name:
Glutathione synthase
Gene namesi
Name:GSH2
Synonyms:GSHII
Ordered Locus Names:At5g27380
ORF Names:F21A20_90
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G27380.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. cytosol Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6161Chloroplast Reviewed prediction
Add
BLAST
Chaini62 – 539478Glutathione synthetase, chloroplastic
PRO_0000013058Add
BLAST

Proteomic databases

PaxDbiP46416.
PRIDEiP46416.

Expressioni

Gene expression databases

GenevestigatoriP46416.

Interactioni

Subunit structurei

Homodimer By similarity.

Protein-protein interaction databases

BioGridi18071. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP46416.
SMRiP46416. Positions 67-538.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni213 – 2164Substrate binding By similarity
Regioni281 – 2833Substrate binding By similarity
Regioni335 – 3384Substrate binding By similarity
Regioni526 – 5272Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiNOG329040.
HOGENOMiHOG000172641.
InParanoidiP46416.
KOiK01920.
OMAiRIPANNA.
PhylomeDBiP46416.

Family and domain databases

Gene3Di1.10.1080.10. 1 hit.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.40.50.1760. 1 hit.
InterProiIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46416-1 [UniParc]FASTAAdd to Basket

« Hide

MGSGCSSLSY SSSSTCNATV FSISSSPSSS SSLKLNPSSF LFQNPKTLRN    50
QSPLRCGRSF KMESQKPIFD LEKLDDEFVQ KLVYDALVWS SLHGLVVGDK 100
SYQKSGNVPG VGLMHAPIAL LPTAFPEAYW KQACNVTPLF NELIDRVSLD 150
GKFLQDSLSR TKKVDVFTSR LLDIHSKMLE RNKKEDIRLG LHRFDYMLDE 200
ETNSLLQIEM NTISCSFPGL SRLVSQLHQS LLRSYGDQIG IDSERVPINT 250
STIQFADALA KAWLEYSNPR AVVMVIVQPE ERNMYDQHLL SSILREKHNI 300
VVIRKTLAEV EKEGSVQEDE TLIVGGQAVA VVYFRSGYTP NDHPSESEWN 350
ARLLIEESSA VKCPSIAYHL TGSKKIQQEL AKPGVLERFL DNKEDIAKLR 400
KCFAGLWSLD DSEIVKQAIE KPGLFVMKPQ REGGGNNIYG DDVRENLLRL 450
QKEGEEGNAA YILMQRIFPK VSNMFLVREG VYHKHQAISE LGVYGAYLRS 500
KDEVIVNEQS GYLMRTKIAS SDEGGVAAGF GVLDSIYLI 539
Length:539
Mass (Da):60,271
Last modified:April 30, 2003 - v3
Checksum:i2BFBF9A1D53E4A96
GO

Sequence cautioni

The sequence AAA99146.1 differs from that shown. Reason: Frameshift at positions 40 and 44.
The sequence AAA64781.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAL11580.1 differs from that shown. Reason: Erroneous initiation.
The sequence CAA58318.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti17 – 171N → S in CAB51026. 1 Publication
Sequence conflicti17 – 171N → S in CAB51027. 1 Publication
Sequence conflicti26 – 261S → P in CAB51026. 1 Publication
Sequence conflicti26 – 261S → P in CAB51027. 1 Publication
Sequence conflicti30 – 345SSSLK → ELRQG in CAA90515. 1 Publication
Sequence conflicti52 – 521S → P1 Publication
Sequence conflicti52 – 521S → P1 Publication
Sequence conflicti96 – 961V → G in CAA58318. 1 Publication
Sequence conflicti101 – 1011S → T1 Publication
Sequence conflicti101 – 1011S → T1 Publication
Sequence conflicti124 – 1241A → G1 Publication
Sequence conflicti124 – 1241A → G1 Publication
Sequence conflicti135 – 1351N → D1 Publication
Sequence conflicti135 – 1351N → D1 Publication
Sequence conflicti151 – 1511G → S in CAB51027. 1 Publication
Sequence conflicti199 – 1991D → G in CAB51027. 1 Publication
Sequence conflicti219 – 2191G → A in CAA58318. 1 Publication
Sequence conflicti438 – 4381I → T in CAB51027. 1 Publication
Sequence conflicti488 – 4881I → M in CAB51027. 1 Publication
Sequence conflicti503 – 5031E → K1 Publication
Sequence conflicti503 – 5031E → K1 Publication
Sequence conflicti539 – 5391I → N1 Publication
Sequence conflicti539 – 5391I → N1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U22359 mRNA. Translation: AAA64781.1. Different initiation.
U53856 Genomic DNA. Translation: AAA99146.1. Frameshift.
AJ243812 Genomic DNA. Translation: CAB51026.1.
AJ243813 mRNA. Translation: CAB51027.1.
AC007123 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED93678.1.
Z50153 mRNA. Translation: CAA90515.1.
X83411 mRNA. Translation: CAA58318.1. Different initiation.
AF424586 mRNA. Translation: AAL11580.1. Different initiation.
BT000624 mRNA. Translation: AAN18190.1.
PIRiS62654.
S68223.
T52565.
RefSeqiNP_568495.2. NM_122620.3.
UniGeneiAt.21515.
At.55036.

Genome annotation databases

EnsemblPlantsiAT5G27380.1; AT5G27380.1; AT5G27380.
GeneIDi832797.
KEGGiath:AT5G27380.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U22359 mRNA. Translation: AAA64781.1 . Different initiation.
U53856 Genomic DNA. Translation: AAA99146.1 . Frameshift.
AJ243812 Genomic DNA. Translation: CAB51026.1 .
AJ243813 mRNA. Translation: CAB51027.1 .
AC007123 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED93678.1 .
Z50153 mRNA. Translation: CAA90515.1 .
X83411 mRNA. Translation: CAA58318.1 . Different initiation.
AF424586 mRNA. Translation: AAL11580.1 . Different initiation.
BT000624 mRNA. Translation: AAN18190.1 .
PIRi S62654.
S68223.
T52565.
RefSeqi NP_568495.2. NM_122620.3.
UniGenei At.21515.
At.55036.

3D structure databases

ProteinModelPortali P46416.
SMRi P46416. Positions 67-538.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 18071. 1 interaction.

Proteomic databases

PaxDbi P46416.
PRIDEi P46416.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G27380.1 ; AT5G27380.1 ; AT5G27380 .
GeneIDi 832797.
KEGGi ath:AT5G27380.

Organism-specific databases

TAIRi AT5G27380.

Phylogenomic databases

eggNOGi NOG329040.
HOGENOMi HOG000172641.
InParanoidi P46416.
KOi K01920.
OMAi RIPANNA.
PhylomeDBi P46416.

Enzyme and pathway databases

UniPathwayi UPA00142 ; UER00210 .
BioCyci ARA:AT5G27380-MONOMER.

Miscellaneous databases

PROi P46416.

Gene expression databases

Genevestigatori P46416.

Family and domain databases

Gene3Di 1.10.1080.10. 1 hit.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.40.50.1760. 1 hit.
InterProi IPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view ]
PANTHERi PTHR11130. PTHR11130. 1 hit.
Pfami PF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view ]
PIRSFi PIRSF001558. GSHase. 1 hit.
SUPFAMi SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR01986. glut_syn_euk. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the cDNA and genomic clones for glutathione synthetase from Arabidopsis thaliana and complementation of a gsh2 mutant in fission yeast."
    Wang C.L., Oliver D.J.
    Plant Mol. Biol. 31:1093-1104(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: cv. RLD.
  2. "Isolation of cDNA and genomic clones of glutathione synthetase containing plastidic targeting sequences from Arabidopsis thaliana."
    Skipsey M., Andrews C.J., Townson J.K., Jepson I., Edwards R.
    Plant Gene Register PGR99-137
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: cv. Columbia.
  3. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Characterisation of an Arabidopsis thaliana cDNA encoding glutathione synthetase."
    Rawlins M.R., Leaver C.J., May M.J.
    FEBS Lett. 376:81-86(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 30-539.
    Strain: cv. C24.
  6. "Cloning of Arabidopsis thaliana glutathione synthetase (GSH2) by functional complementation of a yeast gsh2 mutant."
    Ullmann P., Gondet L., Potier S., Bach T.J.
    Eur. J. Biochem. 236:662-669(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 41-539.
    Strain: cv. Landsberg erecta.
  7. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 20-539.
    Strain: cv. Columbia.

Entry informationi

Entry nameiGSHB_ARATH
AccessioniPrimary (citable) accession number: P46416
Secondary accession number(s): Q8H171, Q9SMW4, Q9SMW5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: April 30, 2003
Last modified: May 14, 2014
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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