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P46413

- GSHB_RAT

UniProt

P46413 - GSHB_RAT

Protein

Glutathione synthetase

Gene

Gss

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei125 – 1251SubstrateBy similarity
    Metal bindingi144 – 1441MagnesiumBy similarity
    Binding sitei144 – 1441ATPBy similarity
    Metal bindingi146 – 1461MagnesiumBy similarity
    Binding sitei220 – 2201SubstrateBy similarity
    Binding sitei305 – 3051ATPBy similarity
    Metal bindingi368 – 3681MagnesiumBy similarity
    Binding sitei375 – 3751ATPBy similarity
    Binding sitei425 – 4251ATPBy similarity
    Binding sitei450 – 4501SubstrateBy similarity
    Binding sitei452 – 4521ATPBy similarity
    Binding sitei458 – 4581ATP; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi364 – 37310ATPBy similarity
    Nucleotide bindingi398 – 4014ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. glutathione binding Source: UniProtKB
    3. glutathione synthase activity Source: RGD
    4. glycine binding Source: RGD
    5. magnesium ion binding Source: UniProtKB
    6. peptide binding Source: RGD
    7. protein homodimerization activity Source: UniProtKB

    GO - Biological processi

    1. aging Source: RGD
    2. glutathione biosynthetic process Source: RGD
    3. response to amino acid Source: RGD
    4. response to cadmium ion Source: Ensembl
    5. response to nutrient levels Source: RGD
    6. response to tumor necrosis factor Source: RGD
    7. response to xenobiotic stimulus Source: RGD

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Glutathione biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00142; UER00210.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione synthetase (EC:6.3.2.3)
    Short name:
    GSH synthetase
    Short name:
    GSH-S
    Alternative name(s):
    Glutathione synthase
    Gene namesi
    Name:Gss
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 3

    Organism-specific databases

    RGDi2752. Gss.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 474473Glutathione synthetasePRO_0000211263Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP46413.
    PRIDEiP46413.

    PTM databases

    PhosphoSiteiP46413.

    Expressioni

    Gene expression databases

    GenevestigatoriP46413.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000025657.

    Structurei

    3D structure databases

    ProteinModelPortaliP46413.
    SMRiP46413. Positions 3-474.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni148 – 1514Substrate bindingBy similarity
    Regioni214 – 2163Substrate bindingBy similarity
    Regioni267 – 2704Substrate bindingBy similarity
    Regioni461 – 4622Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the eukaryotic GSH synthase family.Curated

    Phylogenomic databases

    eggNOGiNOG329040.
    GeneTreeiENSGT00390000013764.
    HOGENOMiHOG000172641.
    HOVERGENiHBG002458.
    InParanoidiP46413.
    KOiK01920.
    OMAiGWLLRSK.
    OrthoDBiEOG757CXD.
    PhylomeDBiP46413.
    TreeFamiTF105187.

    Family and domain databases

    Gene3Di1.10.1080.10. 2 hits.
    3.30.1490.50. 1 hit.
    3.30.1490.80. 2 hits.
    3.40.50.1760. 1 hit.
    InterProiIPR004887. Glutathione_synth_subst-bd_euk.
    IPR014042. Glutathione_synthase_a-hlx_euk.
    IPR014709. Glutathione_synthase_dom.
    IPR005615. Glutathione_synthase_euk.
    IPR014049. Glutathione_synthase_N_euk.
    IPR016185. PreATP-grasp_dom.
    [Graphical view]
    PANTHERiPTHR11130. PTHR11130. 1 hit.
    PfamiPF03917. GSH_synth_ATP. 1 hit.
    PF03199. GSH_synthase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001558. GSHase. 1 hit.
    SUPFAMiSSF52440. SSF52440. 1 hit.
    TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P46413-1 [UniParc]FASTAAdd to Basket

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    MATSWGSILQ DEKQLEELAQ QAIDRALAEG VLLRSAKNPS SSDVVTYAPF    50
    TLFPSPVPST LLEQAYAVQM DFNILVDAVS QNSAFLEQTL SSTIKKDEYT 100
    ARLFDIYKQV LKEGIAQTVF LGLNRSDYMF QCSADGSKAL KQIEINTISA 150
    SFGGLASRTP AVHRHVLNVL NKTNEASKIL SNNPSKGLAL GIAKAWELYG 200
    SANAVVLLIA QEKERNIFDQ RAIENELLDR KIHVIRRRFE DVSERGSLDQ 250
    NRRLFMEDQE VAVVYFRDGY MPSQYNAQNW EARLLLERSC AAKCPDIATQ 300
    LAGTKKVQQE LSRVGLLEAL LPGQPEAVAR LRATFAGLYS LDMGEEGDQA 350
    VAEALAAPSH FVLKPQREGG GNNFYGEEMV HALEQLKDSE ERASYILMEK 400
    IEPEPFRNCL LRPGSPAQVV QCISELGIFG VYVRQGTTLV MNKHVGHLLR 450
    TKAIEHADGG VAAGVAVLDN PYPV 474
    Length:474
    Mass (Da):52,345
    Last modified:November 1, 1995 - v1
    Checksum:i962B742FD19851A4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L38615 mRNA. Translation: AAA64618.1.
    BC078700 mRNA. Translation: AAH78700.1.
    PIRiI59346.
    RefSeqiNP_037094.1. NM_012962.1.
    XP_006235370.1. XM_006235308.1.
    UniGeneiRn.1692.

    Genome annotation databases

    EnsembliENSRNOT00000025657; ENSRNOP00000025657; ENSRNOG00000018964.
    GeneIDi25458.
    KEGGirno:25458.
    UCSCiRGD:2752. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L38615 mRNA. Translation: AAA64618.1 .
    BC078700 mRNA. Translation: AAH78700.1 .
    PIRi I59346.
    RefSeqi NP_037094.1. NM_012962.1.
    XP_006235370.1. XM_006235308.1.
    UniGenei Rn.1692.

    3D structure databases

    ProteinModelPortali P46413.
    SMRi P46413. Positions 3-474.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000025657.

    PTM databases

    PhosphoSitei P46413.

    Proteomic databases

    PaxDbi P46413.
    PRIDEi P46413.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000025657 ; ENSRNOP00000025657 ; ENSRNOG00000018964 .
    GeneIDi 25458.
    KEGGi rno:25458.
    UCSCi RGD:2752. rat.

    Organism-specific databases

    CTDi 2937.
    RGDi 2752. Gss.

    Phylogenomic databases

    eggNOGi NOG329040.
    GeneTreei ENSGT00390000013764.
    HOGENOMi HOG000172641.
    HOVERGENi HBG002458.
    InParanoidi P46413.
    KOi K01920.
    OMAi GWLLRSK.
    OrthoDBi EOG757CXD.
    PhylomeDBi P46413.
    TreeFami TF105187.

    Enzyme and pathway databases

    UniPathwayi UPA00142 ; UER00210 .

    Miscellaneous databases

    NextBioi 606725.
    PROi P46413.

    Gene expression databases

    Genevestigatori P46413.

    Family and domain databases

    Gene3Di 1.10.1080.10. 2 hits.
    3.30.1490.50. 1 hit.
    3.30.1490.80. 2 hits.
    3.40.50.1760. 1 hit.
    InterProi IPR004887. Glutathione_synth_subst-bd_euk.
    IPR014042. Glutathione_synthase_a-hlx_euk.
    IPR014709. Glutathione_synthase_dom.
    IPR005615. Glutathione_synthase_euk.
    IPR014049. Glutathione_synthase_N_euk.
    IPR016185. PreATP-grasp_dom.
    [Graphical view ]
    PANTHERi PTHR11130. PTHR11130. 1 hit.
    Pfami PF03917. GSH_synth_ATP. 1 hit.
    PF03199. GSH_synthase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001558. GSHase. 1 hit.
    SUPFAMi SSF52440. SSF52440. 1 hit.
    TIGRFAMsi TIGR01986. glut_syn_euk. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Amino acid sequence of rat kidney glutathione synthetase."
      Huang C.-S., He W., Meister A., Anderson M.E.
      Proc. Natl. Acad. Sci. U.S.A. 92:1232-1236(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Tissue: Kidney.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.

    Entry informationi

    Entry nameiGSHB_RAT
    AccessioniPrimary (citable) accession number: P46413
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3