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Protein

Excitatory amino acid transporter 1

Gene

SLC1A3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:7723632). Functions as a symporter that transports one amino acid molecule together with two or three Na+ ions and one proton, in parallel with the counter-transport of one K+ ion (By similarity). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi394Sodium 1; via carbonyl oxygenBy similarity1
Metal bindingi396Sodium 2; via carbonyl oxygenBy similarity1
Metal bindingi398Sodium 1By similarity1
Binding sitei402AspartateBy similarity1
Binding sitei476AspartateBy similarity1
Metal bindingi483Sodium 1; via carbonyl oxygenBy similarity1
Binding sitei483AspartateBy similarity1
Metal bindingi487Sodium 1By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAmino-acid transport, Symport, Transport
LigandChloride, Metal-binding, Potassium, Sodium

Enzyme and pathway databases

ReactomeiR-BTA-210455. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
R-BTA-210500. Glutamate Neurotransmitter Release Cycle.
R-BTA-425393. Transport of inorganic cations/anions and amino acids/oligopeptides.

Names & Taxonomyi

Protein namesi
Recommended name:
Excitatory amino acid transporter 1
Alternative name(s):
Sodium-dependent glutamate/aspartate transporter 1
Short name:
GLAST-1
Solute carrier family 1 member 3
Gene namesi
Name:SLC1A3
Synonyms:EAAT1, GLAST1 Publication, GLAST1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 20

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 47CytoplasmicBy similarityAdd BLAST47
Transmembranei48 – 68Helical; Name=1By similarityAdd BLAST21
Topological domaini69 – 86ExtracellularBy similarityAdd BLAST18
Transmembranei87 – 108Helical; Name=2By similarityAdd BLAST22
Topological domaini109 – 122CytoplasmicBy similarityAdd BLAST14
Transmembranei123 – 145Helical; Name=3By similarityAdd BLAST23
Topological domaini146 – 236ExtracellularBy similarityAdd BLAST91
Transmembranei237 – 260Helical; Name=4By similarityAdd BLAST24
Topological domaini261 – 269CytoplasmicBy similarity9
Transmembranei270 – 297Helical; Name=5By similarityAdd BLAST28
Topological domaini298 – 318ExtracellularBy similarityAdd BLAST21
Transmembranei319 – 340Helical; Name=6By similarityAdd BLAST22
Topological domaini341 – 345CytoplasmicBy similarity5
Intramembranei346 – 376Discontinuously helicalBy similarityAdd BLAST31
Topological domaini377 – 385CytoplasmicBy similarity9
Transmembranei386 – 412Helical; Name=7By similarityAdd BLAST27
Topological domaini413 – 425ExtracellularBy similarityAdd BLAST13
Intramembranei426 – 459Discontinuously helicalBy similarityAdd BLAST34
Topological domaini460 – 472ExtracellularBy similarityAdd BLAST13
Transmembranei473 – 494Helical; Name=8By similarityAdd BLAST22
Topological domaini495 – 542CytoplasmicBy similarityAdd BLAST48

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002020561 – 542Excitatory amino acid transporter 1Add BLAST542

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei512PhosphoserineBy similarity1

Post-translational modificationi

Glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP46411.
PRIDEiP46411.

Expressioni

Gene expression databases

BgeeiENSBTAG00000018245.

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000024287.

Structurei

3D structure databases

ProteinModelPortaliP46411.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni363 – 365Aspartate bindingBy similarity3
Regioni443 – 447Aspartate bindingBy similarity5

Domaini

Contains eight transmembrane regions plus two helical hairpins that dip into the membrane. These helical hairpin structures play an important role in the transport process. The first enters the membrane from the cytoplasmic side, the second one from the extracellular side. During the transport cycle, the regions involved in amino acid transport, and especially the helical hairpins, move vertically by about 15-18 Angstroms, alternating between exposure to the aqueous phase and reinsertion in the lipid bilayer. In contrast, the regions involved in trimerization do not move.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3787. Eukaryota.
COG1301. LUCA.
GeneTreeiENSGT00760000119117.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiP46411.
KOiK05614.
OMAiTFAFSSR.
OrthoDBiEOG091G0UCE.
TreeFamiTF315206.

Family and domain databases

Gene3Di1.10.3860.10. 1 hit.
InterProiView protein in InterPro
IPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
PfamiView protein in Pfam
PF00375. SDF. 1 hit.
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiView protein in PROSITE
PS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P46411-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKSNGEEAR LGGRMERFQQ GVRKRTLLAK KKVQNITKED VKSYLFRNAF
60 70 80 90 100
VLLTVTAVIV GTILGFTLRP YRMSYREVKY FSFPGELLMR MLQMLVLPLI
110 120 130 140 150
ISSLVTGMAA LDSKASGKMG MRAVVYYMTT TIIAVVIGII IVIIIHPGKG
160 170 180 190 200
TKENMHREGK IVQVTAADAF LDLIRNMFPP NLVEACFKQF KTNYEKRSFK
210 220 230 240 250
VPIQPNETLV GAVINNVSEA METLTRITEE LVPVPGSVNG VNALGLVVFS
260 270 280 290 300
MCFGFVIGNM KEQGQALREF FDSLNEAIMR LVAVIMWYAP LGILFLIAGK
310 320 330 340 350
IVEMEDMGVI GGQLAMYTVT VIVGLLIHAV IVLPLLYFLV TRKNPWVFIG
360 370 380 390 400
GLLQALITAL GTSSSSATLP ITFKCLEENN GVDKRVTRFV LPVGATINMD
410 420 430 440 450
GTALYEALAA IFIAQVNNFE LNFGQIITIS ITATAASIGA AGIPQAGLVT
460 470 480 490 500
MVIVLTSVGL PTDDITLIIA VDWFLDRLRT TTNVLGDSLG AGIVEHLSRH
510 520 530 540
ELKNRDVEMG NSVIEENEMK KPYQLISQES EIEKSMDSET KM
Length:542
Mass (Da):59,591
Last modified:November 1, 1995 - v1
Checksum:iF23810DB5DC016D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D82056 mRNA. Translation: BAA11527.1.
BC120125 mRNA. Translation: AAI20126.1.
RefSeqiNP_777025.1. NM_174600.2.
UniGeneiBt.361.

Genome annotation databases

EnsembliENSBTAT00000024287; ENSBTAP00000024287; ENSBTAG00000018245.
GeneIDi282354.
KEGGibta:282354.

Similar proteinsi

Entry informationi

Entry nameiEAA1_BOVIN
AccessioniPrimary (citable) accession number: P46411
Secondary accession number(s): Q0VCK3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: August 30, 2017
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families